FastQCFastQC Report
Wed 25 May 2016
SRR1294986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294986_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2612667
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC2159818.266686875901138No Hit
GTACATGGGAAGCAGTGGTATCAAC1929707.385939348566044No Hit
GAGTACATGGGAAGCAGTGGTATCA1065564.078437856795374No Hit
CATGTACTCTGCGTTGATACCACTG946503.6227349294801057No Hit
ACGCAGAGTACATGGGAAGCAGTGG661422.531589368258565No Hit
GCGTTGATACCACTGCTTCCCATGT654932.506748850886852No Hit
ACTCTGCGTTGATACCACTGCTTCC524712.008330950710519No Hit
TATCAACGCAGAGTACATGGGAAGC522371.999374585433199No Hit
GTATCAACGCAGAGTACATGGGAAG467621.7898186029830823No Hit
GGTATCAACGCAGAGTACATGGGAA441031.6880452043831076No Hit
GCTTCCCATGTACTCTGCGTTGATA353361.3524877070059063No Hit
CAGTGGTATCAACGCAGAGTACATG275631.0549756245246715No Hit
GTGGTATCAACGCAGAGTACATGGG245000.937739099548469No Hit
ACATGGGAAGCAGTGGTATCAACGC237660.9096452016273026No Hit
CATGGGAAGCAGTGGTATCAACGCA235410.9010333119375719No Hit
ATACCACTGCTTCCCATGTACTCTG233810.8949093014915411No Hit
GTATCAACGCAGAGTACTTTTTTTT226890.8684229563124578No Hit
GCAGTGGTATCAACGCAGAGTACAT225110.8616099946912484No Hit
GGGAAGCAGTGGTATCAACGCAGAG220860.8453430919439792No Hit
GTACTCTGCGTTGATACCACTGCTT218610.8367312022542481No Hit
GCAGAGTACATGGGAAGCAGTGGTA212160.8120437851436865No Hit
AAGCAGTGGTATCAACGCAGAGTAC202330.7744193959658847No Hit
GTTGATACCACTGCTTCCCATGTAC197410.7555880638443399No Hit
TATCAACGCAGAGTACTTTTTTTTT194280.7436079684092921No Hit
TACCACTGCTTCCCATGTACTCTGC193260.7397039117499474No Hit
GATACCACTGCTTCCCATGTACTCT192690.737522233028549No Hit
CTGCTTCCCATGTACTCTGCGTTGA191770.7340009270220813No Hit
GGTATCAACGCAGAGTACTTTTTTT188150.7201453533879365No Hit
CCACTGCTTCCCATGTACTCTGCGT185010.7081269828876011No Hit
GTACTTTTTTTTTTTTTTTTTTTTT177890.6808751364027639No Hit
CTTCCCATGTACTCTGCGTTGATAC172370.6597473003639576No Hit
ATGGGAAGCAGTGGTATCAACGCAG150540.5761928328409246No Hit
CCATGTACTCTGCGTTGATACCACT146590.561074182052286No Hit
GTACATGGTAAGCAGTGGTATCAAC138430.5298417287775289No Hit
ACGCAGAGTACTTTTTTTTTTTTTT130980.5013268051381979No Hit
TTCCCATGTACTCTGCGTTGATACC122430.4686016243172207No Hit
ACCATGTACTCTGCGTTGATACCAC121850.4663816705305345No Hit
TGATACCACTGCTTCCCATGTACTC105450.40361056345871865No Hit
GAGTACTTTTTTTTTTTTTTTTTTT96950.3710767579641799No Hit
ATCAACGCAGAGTACATGGGAAGCA89240.3415666826273689No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA83470.31948196995637024No Hit
GGAAGCAGTGGTATCAACGCAGAGT68170.26092112006620055No Hit
GGTAAGCAGTGGTATCAACGCAGAG65150.2493620503493174No Hit
CCCCATGTACTCTGCGTTGATACCA64070.24522834329824658No Hit
GTACATGGGTAAGCAGTGGTATCAA58130.22249295451735718No Hit
TCCATGTACTCTGCGTTGATACCAC57730.22096195190584944No Hit
GAGTACATGGTAAGCAGTGGTATCA57700.2208471267099864No Hit
CTGCGTTGATACCACTGCTTCCCAT49690.19018879941454458No Hit
CTCTGCGTTGATACCACTGCTTCCC49230.18842814641131073No Hit
AAAAAGTACTCTGCGTTGATACCAC48820.18685886873451535No Hit
GCTTACCATGTACTCTGCGTTGATA46930.17962488139514143No Hit
GTACATGGAAAGCAGTGGTATCAAC45240.1731563953615214No Hit
AGTGGTATCAACGCAGAGTACATGG43290.16569275763042132No Hit
GCAGAGTACTTTTTTTTTTTTTTTT40860.15639191676551203No Hit
TGGGAAGCAGTGGTATCAACGCAGA40250.15405713778296276No Hit
ACTCTGCGTTGATACCACTGCTTAC39650.1517606338657012No Hit
TCAACGCAGAGTACATGGGAAGCAG39480.15110995775581043No Hit
GAGTACATGGAAAGCAGTGGTATCA39040.14942585488315197No Hit
ACGCAGAGTACATGGTAAGCAGTGG38760.14835415305509658No Hit
GCGTTGATACCACTGCTTACCATGT36880.14115844078101036No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA35970.13767540983983034No Hit
AACGCAGAGTACATGGGAAGCAGTG35940.13756058464396725No Hit
ACTGCTTCCCATGTACTCTGCGTTG35310.13514925553084262No Hit
AAAGTACTCTGCGTTGATACCACTG35170.13461340461681492No Hit
TCCCATGTACTCTGCGTTGATACCA35070.134230653963938No Hit
GTGGTATCAACGCAGAGTACTTTTT34430.13178104978552568No Hit
CTGCTTACCATGTACTCTGCGTTGA32680.12508291336017946No Hit
ATGGTAAGCAGTGGTATCAACGCAG30860.1181168514778194No Hit
TATCAACGCAGAGTACATGGTAAGC29490.11287316753340551No Hit
GCTTTCCATGTACTCTGCGTTGATA28580.10939013659222549No Hit
ATCAACGCAGAGTACTTTTTTTTTT26860.10280682536274235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA6100.016.3709281
GGACGCA1550.015.3219798
CGCAAAA3050.014.32675652
TTGTGCG602.5705333E-514.2494417
TCTTAGG400.00528309514.2488952
CAAGACG400.00528309514.2488954
ACGCAGT2150.013.69713710
CTAAGAC500.001500901213.2989693
TACAAGA1153.783498E-1013.2163662
TGCGCTA655.457273E-513.15332910
TACAAAA7750.013.115112
GTACATA4350.012.89961051
ACGCAAA4800.012.8791131
CGCAGTG2300.012.80384411
GAACAAA7850.012.7213581
CTCTAAT604.0548848E-412.6809731
TGTCCCT751.4800935E-512.66616910
TTAGATA604.0963627E-412.6656854
CTAGTCC604.0963627E-412.6656853
GACGCAG1900.012.499519
Produced by FastQC (version 0.11.5)