FastQCFastQC Report
Wed 25 May 2016
SRR1294985_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294985_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3981049
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC228690.57444658430479No Hit
CCCATGTACTCTGCGTTGATACCAC203920.5122268025337041No Hit
GAGTACATGGGAAGCAGTGGTATCA108470.2724658752002299No Hit
CATGTACTCTGCGTTGATACCACTG80080.20115301268585237No Hit
GTATCAACGCAGAGTACTTTTTTTT76000.19090445759396582No Hit
GCGTTGATACCACTGCTTCCCATGT66550.16716699543261085No Hit
GGTATCAACGCAGAGTACTTTTTTT65240.16387640543987275No Hit
GCTTCCCATGTACTCTGCGTTGATA65110.16354985834135677No Hit
GTATCAACGCAGAGTACATGGGAAG62650.157370582477131No Hit
ACGCAGAGTACATGGGAAGCAGTGG60420.15176904378720282No Hit
GGTATCAACGCAGAGTACATGGGAA56410.1416963217483633No Hit
TATCAACGCAGAGTACATGGGAAGC54220.13619525908874772No Hit
TATCAACGCAGAGTACTTTTTTTTT50210.1261225370499082No Hit
ACTCTGCGTTGATACCACTGCTTCC47140.11841100172341511No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN46270.11622564806411577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC550.003045761712.1036561
CAGTCGA550.003072314212.0896369
TAAACCG600.005858235511.0884485
CGCGATC1502.1445885E-910.76404117
GCGCGAT1606.208211E-910.091289516
TAGCACC2750.010.0231834
GACCGTG1252.5758145E-69.8790837
GGCGCGA1951.6552804E-109.74109215
TATACCG800.00450089859.5043845
AACTGCG900.00111526269.4991187
CCCGTCC900.00111539119.4989998
ATACCGT900.00111551969.4988796
CTAGGAC4650.09.4014763
TCTGTCG3250.09.352868
GTCTCGC2357.2759576E-129.3076731
TGTCGCC3250.09.06024210
CCGTGGC1054.5001012E-49.0466669
TAGACAG5700.09.0041535
AGTACGG950.00181836459.0034723
CCGCGCC1804.2413376E-88.97003519
Produced by FastQC (version 0.11.5)