##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294985_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3981049
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.126543782806994	33.0	33.0	33.0	27.0	33.0
2	31.16275584651181	33.0	33.0	33.0	27.0	33.0
3	31.104918577992887	33.0	33.0	33.0	27.0	33.0
4	34.62439673563425	37.0	37.0	37.0	27.0	37.0
5	34.94482534628436	37.0	37.0	37.0	33.0	37.0
6	34.92206576708802	37.0	37.0	37.0	33.0	37.0
7	34.80385998765652	37.0	37.0	37.0	33.0	37.0
8	34.68868481648932	37.0	37.0	37.0	27.0	37.0
9	34.5998979163532	37.0	37.0	37.0	27.0	37.0
10	34.524048058690056	37.0	37.0	37.0	27.0	37.0
11	34.70649293691186	37.0	37.0	37.0	27.0	37.0
12	34.60641855953042	37.0	37.0	37.0	27.0	37.0
13	34.53547921665872	37.0	37.0	37.0	27.0	37.0
14	35.912807905655015	40.0	37.0	40.0	27.0	40.0
15	36.07935747588136	40.0	37.0	40.0	27.0	40.0
16	36.16563473597034	40.0	37.0	40.0	27.0	40.0
17	36.082774163292136	40.0	37.0	40.0	27.0	40.0
18	36.02022230824087	40.0	37.0	40.0	27.0	40.0
19	36.11877120829209	40.0	37.0	40.0	27.0	40.0
20	36.12511501365595	40.0	37.0	40.0	27.0	40.0
21	36.129673862341306	40.0	37.0	40.0	27.0	40.0
22	36.02996345937968	40.0	37.0	40.0	27.0	40.0
23	35.928245796522475	40.0	37.0	40.0	27.0	40.0
24	35.99163411452609	40.0	37.0	40.0	27.0	40.0
25	34.83654785459812	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.3272586100831312
1101	2	0.36278112230777637
1101	3	0.3695288389569207
1101	4	0.4389468728787165
1101	5	0.33869798990838973
1101	6	0.2797653792500583
1101	7	0.28525443237054304
1101	8	0.42769501658690956
1101	9	0.472470784255151
1101	10	0.6652143967798736
1101	11	0.5463429472937804
1101	12	0.5346429335398497
1101	13	0.5408748532958043
1101	14	0.8900637079865419
1101	15	0.7460407775510447
1101	16	0.6743123554138393
1101	17	0.7123150067900568
1101	18	0.7467840360482398
1101	19	0.6157414762757156
1101	20	0.6100784085577402
1101	21	0.682293560213374
1101	22	0.6823714236591059
1101	23	0.7614045966246721
1101	24	0.655085911824159
1101	25	0.6342824620010319
1102	1	0.3783970576375424
1102	2	0.3404188888634252
1102	3	0.40350944677545186
1102	4	0.40180095966734086
1102	5	0.3518880891561764
1102	6	0.16455384989040311
1102	7	0.1727896221311056
1102	8	0.29185856050155934
1102	9	0.3814509940404065
1102	10	0.5573504170549697
1102	11	0.3377836396111604
1102	12	0.39060380759198665
1102	13	0.36008176836418926
1102	14	0.7019536134283086
1102	15	0.6351442184402103
1102	16	0.5693783348579586
1102	17	0.6102329308800449
1102	18	0.6429086392814227
1102	19	0.5718951431754107
1102	20	0.5662827342387047
1102	21	0.5593185002550882
1102	22	0.5721133099315878
1102	23	0.6889185386449697
1102	24	0.6207647322561485
1102	25	0.6147663836275541
1103	1	0.34301692174632237
1103	2	0.3291143195311328
1103	3	0.2885709829039662
1103	4	0.37303167655798575
1103	5	0.22867778832235075
1103	6	0.19896978061689197
1103	7	0.24279671600940844
1103	8	0.4818030305990675
1103	9	0.423521696550182
1103	10	0.5065663986710902
1103	11	0.4054358101164155
1103	12	0.47464959576502963
1103	13	0.4286034643293064
1103	14	0.9037964882835468
1103	15	0.7048252438208422
1103	16	0.6472766157993917
1103	17	0.6104635529205922
1103	18	0.6838002188081163
1103	19	0.6172544461237734
1103	20	0.6376815118652104
1103	21	0.6830221067737341
1103	22	0.6885984551005109
1103	23	0.6862074162502978
1103	24	0.6179139189591893
1103	25	0.7648330626758479
1104	1	0.24213438433325152
1104	2	0.2684023539190683
1104	3	0.3088462016462792
1104	4	0.2586288695882786
1104	5	0.10825223784786431
1104	6	0.1777809445419436
1104	7	0.18966720804758808
1104	8	0.2942544401945142
1104	9	0.36299394561290654
1104	10	0.4312551678098515
1104	11	0.3309031469373025
1104	12	0.439092968084303
1104	13	0.42567643554046697
1104	14	0.7259561121492482
1104	15	0.6077185466363559
1104	16	0.44983425099871965
1104	17	0.4881143437879416
1104	18	0.5271402696796201
1104	19	0.43361596075185105
1104	20	0.5589495277352583
1104	21	0.4705529596484368
1104	22	0.6195745170594265
1104	23	0.5361996014220338
1104	24	0.5058518044168991
1104	25	0.690142533238614
1105	1	0.27186556080622637
1105	2	0.1859691204804328
1105	3	0.18139310416716725
1105	4	0.2658613786664432
1105	5	0.20343725256172007
1105	6	0.12227236247441198
1105	7	0.13093131079538978
1105	8	0.19653403991280527
1105	9	0.20331731696209943
1105	10	0.36359132270656147
1105	11	0.24574207180540952
1105	12	0.25778814596851163
1105	13	0.2988884015465345
1105	14	0.5375995218702556
1105	15	0.39408706570308993
1105	16	0.35249622842711403
1105	17	0.1905959858278834
1105	18	0.32225064498540235
1105	19	0.34184356431093477
1105	20	0.2990282088909382
1105	21	0.38637917261203825
1105	22	0.25598648378642963
1105	23	0.4008841333340456
1105	24	0.38994708827870284
1105	25	0.22077319005188656
1106	1	0.052287462853300326
1106	2	-0.03110324331355585
1106	3	-0.17396578672162022
1106	4	-0.12285491132076487
1106	5	-0.488758621714517
1106	6	-0.3662059730741163
1106	7	-0.7322308924612813
1106	8	-0.25635901909951286
1106	9	0.07406238922159503
1106	10	-0.3063291263041563
1106	11	-0.32339186207163095
1106	12	-0.784242736675715
1106	13	-0.19426684990961718
1106	14	-0.34388280486341216
1106	15	-0.08760501115808239
1106	16	-0.08988193325463811
1106	17	-0.3329683692813816
1106	18	-0.44308290747557066
1106	19	-0.6670367269278827
1106	20	-0.15098891942695758
1106	21	0.06954069647733263
1106	22	0.14996135874492467
1106	23	-0.6599423271421259
1106	24	0.05474028537014419
1106	25	0.1261425828003908
1107	1	-1.1006117637874056
1107	2	-1.030664597353148
1107	3	-0.8330348885393803
1107	4	-0.9299390868289663
1107	5	-1.0271739628711671
1107	6	-1.0661693683080244
1107	7	-1.0878486169180448
1107	8	-0.9714486361933581
1107	9	-0.9902141499834514
1107	10	-0.8333745012216127
1107	11	-0.9118325636191571
1107	12	-0.9625938661068929
1107	13	-0.8398334753123038
1107	14	-1.1751906514075543
1107	15	-1.0200143221004652
1107	16	-1.036181911042668
1107	17	-1.0387265965312409
1107	18	-1.2988074823286269
1107	19	-1.2027684105326202
1107	20	-1.2624263865155356
1107	21	-1.2089061312364677
1107	22	-1.2188711591117212
1107	23	-0.8417172823318495
1107	24	-0.7835751711364978
1107	25	-0.815131966553885
1108	1	-0.08888590039995847
1108	2	-0.12475013822657388
1108	3	-0.07398291099408993
1108	4	-0.22464109730578485
1108	5	-0.4697915625570488
1108	6	-0.06733649019338372
1108	7	0.006693057198418728
1108	8	0.15306019175683616
1108	9	-0.22442656677628747
1108	10	-0.5097448024585631
1108	11	-0.10763055901072249
1108	12	0.003916732112806187
1108	13	0.08034274987987544
1108	14	-0.2567384345847543
1108	15	0.03746723098711868
1108	16	-0.7663430872674013
1108	17	-0.6605589092997874
1108	18	-0.5904185327489984
1108	19	-0.07004544978354943
1108	20	-0.9622584746089302
1108	21	-0.3160773231869385
1108	22	-0.29702061625600606
1108	23	-0.19285604545701318
1108	24	-0.379406898286355
1108	25	-1.0292672804429088
1109	1	0.2413395561900913
1109	2	0.17951786721319607
1109	3	0.24571850896345282
1109	4	0.2121387288135992
1109	5	0.10957280452105067
1109	6	0.10991265837543551
1109	7	0.19846399790326785
1109	8	0.3032635689127332
1109	9	0.270740306603372
1109	10	0.31782974014900844
1109	11	0.24809040821489958
1109	12	0.4132492480048384
1109	13	0.2910193980079967
1109	14	0.6462533886743742
1109	15	0.51464784504779
1109	16	0.40368636171901073
1109	17	0.44559543207675745
1109	18	0.47529782373921137
1109	19	0.420128340197536
1109	20	0.381819476719933
1109	21	0.5063791473458252
1109	22	0.49076195981237447
1109	23	0.5393988191087047
1109	24	0.5593417161768954
1109	25	0.583682413187482
1110	1	-0.032283295677551394
1110	2	-0.045972409946877235
1110	3	0.01887836845351032
1110	4	-1.0154743467488814
1110	5	-0.25820470655218486
1110	6	-0.0799240921760287
1110	7	-0.0049025701454255
1110	8	0.0763999309436798
1110	9	0.09418866916128366
1110	10	-0.1046438719125149
1110	11	-0.12248850593394423
1110	12	0.06789215619438238
1110	13	0.0980937288874486
1110	14	0.33334886629403826
1110	15	0.10600484714952785
1110	16	0.12083450575541832
1110	17	0.14141338057441288
1110	18	0.23263012558436458
1110	19	0.11077665309456108
1110	20	0.03446009003961592
1110	21	0.09582443898529647
1110	22	-0.504678560140178
1110	23	0.044313971875752145
1110	24	0.1326744157761368
1110	25	0.0940906686475742
1111	1	-0.10214219326276464
1111	2	-0.3100003593270735
1111	3	-0.177396499566413
1111	4	-0.12860457852713836
1111	5	-0.1613641244648747
1111	6	-0.053561052486244876
1111	7	-0.11640195091654704
1111	8	0.021127456853584192
1111	9	-0.08981750925596543
1111	10	-0.0740501690406532
1111	11	-0.1709621170981208
1111	12	-0.09152792239621732
1111	13	-0.1844222604563157
1111	14	-0.3492215626250754
1111	15	-0.17443211987741591
1111	16	-0.14127421131770035
1111	17	-0.029521094083683863
1111	18	-0.16175047883850624
1111	19	-0.19990720482909552
1111	20	-0.10478763144391934
1111	21	-0.21951189756955358
1111	22	-0.24493819578683684
1111	23	-0.060683274508711804
1111	24	-0.01017558968729304
1111	25	-0.1220055830525979
1112	1	-0.10588747761386941
1112	2	-0.3189313444185622
1112	3	-0.09417916615238298
1112	4	-0.16725978641916583
1112	5	-0.06060861194697509
1112	6	-0.11953978919668629
1112	7	-0.02172685156009635
1112	8	0.03742292377847889
1112	9	-0.030953098031204718
1112	10	0.2465393122884052
1112	11	-0.03192864859966704
1112	12	0.06103113669313842
1112	13	-0.002059291665339913
1112	14	-0.14584344323594678
1112	15	-0.09261082027484235
1112	16	-0.08810388308836536
1112	17	-0.1609873330636944
1112	18	-0.13691842654775854
1112	19	0.004523992088330431
1112	20	-0.8099279409115283
1112	21	-1.2156122828910227
1112	22	-1.5588924817316823
1112	23	-0.46321610752934816
1112	24	-0.31835610573562434
1112	25	-1.2685901419502912
1113	1	-0.13795495101452104
1113	2	-0.16684412046300068
1113	3	-0.20615593619214323
1113	4	-0.4749467451835585
1113	5	-0.21506129084909276
1113	6	-0.09866176701627438
1113	7	-0.12256765965877747
1113	8	-0.0071220082279737085
1113	9	-0.11790794469479238
1113	10	0.001961028993569869
1113	11	-0.07870249332520274
1113	12	-0.07745202878505353
1113	13	-0.12447565105777869
1113	14	-0.43198802851621565
1113	15	-0.4201383983352187
1113	16	-0.3385748055933817
1113	17	-0.39742693340336643
1113	18	-0.7984014038015133
1113	19	-1.020633389273165
1113	20	-1.180884042982953
1113	21	-1.0662347969044959
1113	22	-0.41815785129778504
1113	23	-1.9770986716723868
1113	24	-1.0342780137011047
1113	25	-0.4701119408554675
1114	1	-0.4187518883386794
1114	2	-0.5363043239167062
1114	3	-0.46972882273272276
1114	4	-0.6550355331355746
1114	5	-0.7621854493519464
1114	6	-0.5392280064481199
1114	7	-0.47716775212294493
1114	8	-0.2170809524107753
1114	9	-1.254794309596143
1114	10	-0.44067154488742233
1114	11	-0.5712913210593058
1114	12	-0.4925818516907796
1114	13	-0.4486531644420637
1114	14	-0.8638912507659455
1114	15	-1.4274015812512602
1114	16	-1.354603625785515
1114	17	-1.6953750053726537
1114	18	-1.9551691919268777
1114	19	-1.8347115931400424
1114	20	-1.0365945329560589
1114	21	-0.7920896360784369
1114	22	-0.5110720363580938
1114	23	-0.4346267183947319
1114	24	-0.983947065666122
1114	25	-0.6018332829565622
1115	1	-0.5890535955633887
1115	2	-0.487109578720883
1115	3	-0.430576400412221
1115	4	-0.5180809191062323
1115	5	-0.2773563146403859
1115	6	-0.2587496441223891
1115	7	-0.32390346133200154
1115	8	-0.11338795082198772
1115	9	-0.461834405778184
1115	10	-0.189707108096556
1115	11	-0.4518085632660984
1115	12	-0.47919389734332896
1115	13	-0.4485581314633649
1115	14	-0.3739715262576766
1115	15	-1.4494875576965924
1115	16	-0.7690375766072179
1115	17	-0.6529917879497305
1115	18	-2.0965912878795763
1115	19	-1.0541439291699461
1115	20	-0.8787277943075651
1115	21	-0.7999047997073347
1115	22	-0.6695642825849788
1115	23	-0.32360683490483666
1115	24	-0.345026464170509
1115	25	-0.5129963892298051
1116	1	-1.506188114365191
1116	2	-0.9303267812920168
1116	3	-1.6874997878596005
1116	4	-1.0087220687825749
1116	5	-1.0727783521625511
1116	6	-0.4949773646578137
1116	7	-1.239438924925203
1116	8	-2.9965630315353273
1116	9	-1.4784988634707545
1116	10	-0.8448567322370124
1116	11	-0.6563918107374391
1116	12	-0.7734884389468917
1116	13	-1.67731847787217
1116	14	-1.8385235582595527
1116	15	-1.6152124538395753
1116	16	-0.9793553177697376
1116	17	-1.6974496588308128
1116	18	-2.542840881854204
1116	19	-1.43784842836304
1116	20	-1.0997834933596309
1116	21	-0.8440458467169378
1116	22	-0.6752452829506055
1116	23	-0.5354422423759999
1116	24	-0.601879905877766
1116	25	-0.43136745864226356
1201	1	0.31605115424380514
1201	2	0.29389725856468374
1201	3	0.2931676064315454
1201	4	0.2917610909909172
1201	5	0.27681134467485435
1201	6	0.09256037979191234
1201	7	0.2534989145437194
1201	8	0.34122548655042095
1201	9	0.2956710154439719
1201	10	0.4611678700306072
1201	11	0.3390373807005602
1201	12	0.47192400856784644
1201	13	0.39408151442357564
1201	14	0.8486067849651917
1201	15	0.6791395024002327
1201	16	0.6147271948459831
1201	17	0.5537061854917908
1201	18	0.4768031813720839
1201	19	0.5179210088408226
1201	20	0.5390116889103069
1201	21	0.5278170945124074
1201	22	0.5410951169004434
1201	23	0.7456976284492569
1201	24	0.6704091065646125
1201	25	0.8059459377803861
1202	1	0.315263328074721
1202	2	0.3486571785004564
1202	3	0.3571045722008179
1202	4	0.4203065739373102
1202	5	0.25054963927778573
1202	6	-0.03217326135035137
1202	7	0.13418049214317307
1202	8	0.1571437658234487
1202	9	0.14497598967012237
1202	10	0.3060029646073801
1202	11	0.17037838382830017
1202	12	0.2258769944476242
1202	13	0.14946220092708273
1202	14	0.5380693883602916
1202	15	0.37426359105210594
1202	16	0.2748692616218733
1202	17	0.3197558442016799
1202	18	0.3874016650922343
1202	19	0.3423712284362139
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2210	22	0.42609948772486206
2210	23	0.6411742058180963
2210	24	0.6748358722857972
2210	25	0.6916686988813652
2211	1	0.1030812298835393
2211	2	-0.0255227522505308
2211	3	0.11175417820917843
2211	4	0.11286979723770685
2211	5	0.16949681557058227
2211	6	0.08878552316024724
2211	7	0.1339607909164826
2211	8	0.20597248399349155
2211	9	0.24655448485761156
2211	10	0.2717231177457293
2211	11	0.11668473798314238
2211	12	0.21545313286936363
2211	13	0.12649203856086189
2211	14	0.05394573508836231
2211	15	0.15472999302370738
2211	16	0.14161079778077834
2211	17	0.1574377278613852
2211	18	0.17261355463046613
2211	19	0.07671312308152523
2211	20	0.08866032799595303
2211	21	0.07988617835701461
2211	22	0.14048436444198842
2211	23	0.21136552128518105
2211	24	0.25934797390467423
2211	25	0.18766502262640472
2212	1	0.15257493150101809
2212	2	0.09846669951357967
2212	3	0.07315898479614447
2212	4	0.12483701429966487
2212	5	0.14226855593718568
2212	6	0.21352210515151882
2212	7	0.23666300310907928
2212	8	0.2962924913710552
2212	9	0.0519252762484399
2212	10	0.27403488646721286
2212	11	0.08054397276685421
2212	12	0.1305530910993511
2212	13	0.056254992569407136
2212	14	0.1773130621094694
2212	15	0.0955976881138696
2212	16	0.05902799699364891
2212	17	0.13052433739003533
2212	18	0.25137219640186714
2212	19	0.20497869506750277
2212	20	0.20896580580573954
2212	21	0.2739896691934476
2212	22	-0.20773266335110918
2212	23	0.1682595190138585
2212	24	0.12074425328084004
2212	25	0.2329970932473202
2213	1	0.03151577691567198
2213	2	-0.7460903333056734
2213	3	-0.07558854292938477
2213	4	-0.040753769160190245
2213	5	0.011784069958345356
2213	6	-0.053509409501572236
2213	7	0.011942955681043088
2213	8	0.1453510866842862
2213	9	-0.13908981914433127
2213	10	-1.3962359476574093
2213	11	-0.3655406270480839
2213	12	-0.052864628468618946
2213	13	-0.04994340480774184
2213	14	0.09214984658107994
2213	15	0.06839895330188028
2213	16	-0.050386627861925604
2213	17	-0.10860687522309576
2213	18	0.04730117985324256
2213	19	0.055073677747131455
2213	20	0.06777409382206656
2213	21	0.036755633081867245
2213	22	0.21223403904071603
2213	23	-0.758417622995573
2213	24	-1.3843087372010743
2213	25	-1.3369975177035158
2214	1	0.014937849962851857
2214	2	-0.2231172249010953
2214	3	-0.0098217552705826
2214	4	0.04313714841209304
2214	5	-0.04350049844229886
2214	6	-0.0849230859860981
2214	7	-0.03399506135989583
2214	8	0.13619267445096028
2214	9	-0.20193703377417904
2214	10	0.11742331735180755
2214	11	-0.07308322939940837
2214	12	-0.054410649212208284
2214	13	-0.02681440969844573
2214	14	0.0016557467698561368
2214	15	0.005566316037764807
2214	16	-0.1285278679584252
2214	17	-0.061880587130616505
2214	18	0.12553043079530823
2214	19	-0.6433304651402025
2214	20	-0.2206672395695719
2214	21	-0.17727544586185928
2214	22	-0.3013763006847938
2214	23	-1.6175228278439988
2214	24	-1.1030750000747105
2214	25	-1.4199563949548093
2215	1	-0.4099693343298725
2215	2	-0.3086685412777115
2215	3	-1.2049884219372515
2215	4	-0.7488441099547103
2215	5	-0.2965881825119112
2215	6	-0.2049431663073804
2215	7	-0.10042701138220878
2215	8	-0.05233164504926435
2215	9	-0.2123605054386175
2215	10	-1.1959700338306973
2215	11	-0.408310137029936
2215	12	-0.4262225635361716
2215	13	-0.45425260693182423
2215	14	-0.7321969975407185
2215	15	-0.9752560846762037
2215	16	-0.6988330888419583
2215	17	-0.5279707254617207
2215	18	-0.4262701046799293
2215	19	-0.9441310006154424
2215	20	-1.1913082480479318
2215	21	-0.6440536698689954
2215	22	-0.33492694771736353
2215	23	-0.637662497634274
2215	24	-1.646054250409378
2215	25	-0.6758381441739019
2216	1	-0.5446360009965403
2216	2	-0.47948335609252624
2216	3	-1.408840273789103
2216	4	-0.717140406251005
2216	5	-0.561921515845242
2216	6	-0.4951653885295997
2216	7	-0.356427011382209
2216	8	-0.5544057191233378
2216	9	-0.27458272766083525
2216	10	-0.3648589227195913
2216	11	-0.40712495184475017
2216	12	-0.2397040450176533
2216	13	-1.1379563106355306
2216	14	-0.7366414419851637
2216	15	-1.171700529120649
2216	16	-0.8954256814345527
2216	17	-1.8207114662024608
2216	18	-0.8544182528280757
2216	19	-0.5093161858006283
2216	20	-0.5490860258257086
2216	21	-0.37146107727640754
2216	22	-0.4115195403099605
2216	23	-0.2963291643009427
2216	24	-0.3900542504093849
2216	25	-0.31183814417390465
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5145.0
3	560.0
4	617.0
5	576.0
6	406.0
7	310.0
8	552.0
9	1195.0
10	2276.0
11	4181.0
12	7191.0
13	10404.0
14	14043.0
15	17937.0
16	20298.0
17	21256.0
18	22173.0
19	22894.0
20	23191.0
21	25567.0
22	26507.0
23	28392.0
24	29557.0
25	31686.0
26	32606.0
27	36912.0
28	41011.0
29	48475.0
30	58299.0
31	74261.0
32	97822.0
33	137369.0
34	199388.0
35	316238.0
36	580236.0
37	2041518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.04696030906756	21.081444179799593	13.930238072021531	22.941357439111318
2	14.706182843050907	20.94473942474647	40.99167065099776	23.35740708120486
3	18.225113291578104	26.602536271552456	29.97539738570481	25.196953051164627
4	12.050928841904296	18.551412432791945	38.3675874780924	31.03007124721136
5	12.311912906519547	39.28339500398478	35.41809931849629	12.986592770999392
6	30.15666598041826	39.11633521204321	17.685879776326647	13.041119031211887
7	25.493601030036352	33.59061591784675	23.06616513802105	17.849617914095855
8	23.972785327759432	37.80330230131026	21.0145440110075	17.2093683599228
9	23.764311293316503	18.03647117401818	22.211786751219993	35.987430781445326
10	14.389448565128415	31.224899219256052	33.86761666033522	20.518035555280314
11	31.335463959975495	26.443093492695752	23.128205567046532	19.093236980282224
12	20.53842459609094	28.584664594641122	31.51932934030452	19.357581468963414
13	26.359174970704586	24.272687109892725	26.55902569311498	22.80911222628771
14	19.87546684829639	24.590414400699938	28.362427817538645	27.171690933465026
15	23.065725175623516	31.013255573743752	24.13753702238357	21.78348222824916
16	20.748208659995374	31.959106430349976	26.726146025954765	20.56653888369988
17	20.016963805933052	31.115798110574318	28.603770545648118	20.263467537844516
18	20.173466506048598	30.378435765945312	29.621221922251927	19.82687580575416
19	20.52199389131986	29.83812123477392	29.99573374007528	19.64415113383094
20	21.586829775961725	29.271255824957432	29.2345348402612	19.907379558819642
21	21.41719556864954	29.60965196412275	28.796781336341393	20.176371130886313
22	20.60013873130324	29.350555277578383	29.13636743458839	20.912938556529987
23	20.26172234556904	29.800334206815073	29.10672544021642	20.831218007399464
24	20.62603024294853	29.710167547586273	29.0308239627587	20.632978246706497
25	21.11210289591093	28.963237507790794	28.094846847282255	21.82981274901602
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9483.0
1	9483.0
2	8317.5
3	7152.0
4	7152.0
5	7152.0
6	9595.0
7	12038.0
8	12038.0
9	12038.0
10	16680.0
11	21322.0
12	21322.0
13	21322.0
14	33354.0
15	45386.0
16	45386.0
17	45386.0
18	69156.0
19	92926.0
20	92926.0
21	92926.0
22	129981.5
23	167037.0
24	167037.0
25	167037.0
26	211962.0
27	256887.0
28	256887.0
29	256887.0
30	302145.5
31	347404.0
32	347404.0
33	347404.0
34	381614.5
35	415825.0
36	415825.0
37	415825.0
38	433859.0
39	451893.0
40	451893.0
41	451893.0
42	450273.0
43	448653.0
44	448653.0
45	448653.0
46	474128.0
47	499603.0
48	499603.0
49	499603.0
50	457364.0
51	415125.0
52	415125.0
53	415125.0
54	362325.5
55	309526.0
56	309526.0
57	309526.0
58	264172.0
59	218818.0
60	218818.0
61	218818.0
62	178830.5
63	138843.0
64	138843.0
65	138843.0
66	105919.0
67	72995.0
68	72995.0
69	72995.0
70	52520.0
71	32045.0
72	32045.0
73	32045.0
74	22154.5
75	12264.0
76	12264.0
77	12264.0
78	8190.0
79	4116.0
80	4116.0
81	4116.0
82	2688.0
83	1260.0
84	1260.0
85	1260.0
86	796.0
87	332.0
88	332.0
89	332.0
90	208.0
91	84.0
92	84.0
93	84.0
94	51.5
95	19.0
96	19.0
97	19.0
98	16.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30665284451409663
2	0.1795757851762186
3	0.1982894458219429
4	0.21810834280110591
5	0.24325246938683748
6	0.19479790376857958
7	0.19082910057123134
8	0.21315989830820972
9	0.2131850173157879
10	0.22134869477868774
11	0.19801313673858323
12	0.20409193657249633
13	0.21562156105086874
14	0.2042426506179653
15	0.19535052193529898
16	0.19859087391288074
17	0.19803825574616138
18	0.2025345581026508
19	0.20130372673132133
20	0.19703349544303522
21	0.19399409552607869
22	0.19846527887499
23	0.20245920107991636
24	0.21815858081626224
25	0.21132621075500452
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3981049.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.34205361943086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.50857058694724	71.0179380690818
2	8.283990719598735	13.145376717145407
3	1.2844597034854675	3.057350119978266
4	0.3530016575635146	1.1203150576860936
5	0.15701573646034894	0.6228975490665715
6	0.0900791895517738	0.42882407324470273
7	0.05337347585361839	0.2964332828123232
8	0.038696812446693096	0.2456227654437266
9	0.030825580627590114	0.2201188383903909
>10	0.17528447848475268	2.5162295327915736
>50	0.013420313075337923	0.7466478437696407
>100	0.008989269234219386	1.3491250487055624
>500	7.004790640239912E-4	0.3643606810168217
>1k	0.0011780782289687345	1.892917019076577
>5k	3.183995213428987E-4	1.6089415725072411
>10k+	9.55198564028696E-5	1.366901829283264
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	22869	0.57444658430479	No Hit
CCCATGTACTCTGCGTTGATACCAC	20392	0.5122268025337041	No Hit
GAGTACATGGGAAGCAGTGGTATCA	10847	0.2724658752002299	No Hit
CATGTACTCTGCGTTGATACCACTG	8008	0.20115301268585237	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7600	0.19090445759396582	No Hit
GCGTTGATACCACTGCTTCCCATGT	6655	0.16716699543261085	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6524	0.16387640543987275	No Hit
GCTTCCCATGTACTCTGCGTTGATA	6511	0.16354985834135677	No Hit
GTATCAACGCAGAGTACATGGGAAG	6265	0.157370582477131	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	6042	0.15176904378720282	No Hit
GGTATCAACGCAGAGTACATGGGAA	5641	0.1416963217483633	No Hit
TATCAACGCAGAGTACATGGGAAGC	5422	0.13619525908874772	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5021	0.1261225370499082	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4714	0.11841100172341511	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	4627	0.11622564806411577	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.5119007578153397E-5	0.0	0.0	2.5119007578153397E-5	0.0
6	5.0238015156306795E-5	0.0	0.0	2.5119007578153397E-5	0.0
7	5.0238015156306795E-5	0.0	0.0	2.5119007578153397E-5	0.0
8	5.0238015156306795E-5	0.0	0.0	2.5119007578153397E-5	0.0
9	5.0238015156306795E-5	0.0	0.0	1.0047603031261359E-4	0.0
10	5.0238015156306795E-5	0.0	0.0	1.7583305304707378E-4	0.0
11	5.0238015156306795E-5	0.0	0.0	2.0095206062522718E-4	0.0
12	5.0238015156306795E-5	0.0	0.0	2.0095206062522718E-4	0.0
13	5.0238015156306795E-5	0.0	0.0	2.2607106820338058E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAC	55	0.0030457617	12.103656	1
CAGTCGA	55	0.0030723142	12.089636	9
TAAACCG	60	0.0058582355	11.088448	5
CGCGATC	150	2.1445885E-9	10.764041	17
GCGCGAT	160	6.208211E-9	10.0912895	16
TAGCACC	275	0.0	10.023183	4
GACCGTG	125	2.5758145E-6	9.879083	7
GGCGCGA	195	1.6552804E-10	9.741092	15
TATACCG	80	0.0045008985	9.504384	5
AACTGCG	90	0.0011152626	9.499118	7
CCCGTCC	90	0.0011153911	9.498999	8
ATACCGT	90	0.0011155196	9.498879	6
CTAGGAC	465	0.0	9.401476	3
TCTGTCG	325	0.0	9.35286	8
GTCTCGC	235	7.2759576E-12	9.307673	1
TGTCGCC	325	0.0	9.060242	10
CCGTGGC	105	4.5001012E-4	9.046666	9
TAGACAG	570	0.0	9.004153	5
AGTACGG	95	0.0018183645	9.003472	3
CCGCGCC	180	4.2413376E-8	8.970035	19
>>END_MODULE