FastQCFastQC Report
Wed 25 May 2016
SRR1294985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294985_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3981049
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC229620.5767826520095584No Hit
GTACATGGGAAGCAGTGGTATCAAC224760.5645748143265757No Hit
GTATCAACGCAGAGTACTTTTTTTT172660.43370478484439656No Hit
GGTATCAACGCAGAGTACTTTTTTT156920.3941674669163831No Hit
TATCAACGCAGAGTACTTTTTTTTT119130.29924273727854145No Hit
GAGTACATGGGAAGCAGTGGTATCA119070.2990920232330725No Hit
CATGTACTCTGCGTTGATACCACTG86950.21840977089204378No Hit
ACGCAGAGTACATGGGAAGCAGTGG74280.18658398829052344No Hit
GCGTTGATACCACTGCTTCCCATGT71960.18075637853239185No Hit
GCTTCCCATGTACTCTGCGTTGATA71930.1806810215096574No Hit
ACGCAGAGTACTTTTTTTTTTTTTT67490.16952818214495727No Hit
GTATCAACGCAGAGTACATGGGAAG66610.16731770947807978No Hit
GGTATCAACGCAGAGTACATGGGAA62590.1572198684316621No Hit
TATCAACGCAGAGTACATGGGAAGC59130.14852869180962103No Hit
ACTCTGCGTTGATACCACTGCTTCC55150.13853132679351599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATGC400.005283151314.2491339
TACACCG707.272991E-613.5707735
ATACCGT907.474744E-611.6102596
ACACCGT1053.4731002E-610.8563466
CTACGTG803.7755846E-410.6868519
TATTCCG1909.094947E-1210.4994935
TCCGTAC1201.7031814E-59.4994238
TAGACAG5550.09.413965
CCGTACC1252.7583672E-59.1194459
GTATACG1151.0979654E-49.0971441
AAGATCG950.00182556238.9995655
TTACACT6950.08.8844634
GTCCTAA3050.08.731091
TTCCGTA1651.0520198E-68.63583857
AAGCGTT1107.152834E-48.63583857
CACCGTC1107.152834E-48.63583857
CTTACAC5650.08.5748083
GTCTTAG3450.08.5458021
TATACTG8600.08.50540355
GCACCGT1804.0430132E-78.4438256
Produced by FastQC (version 0.11.5)