FastQCFastQC Report
Wed 25 May 2016
SRR1294984_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294984_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3046951
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC142060.46623657551434206No Hit
CCCATGTACTCTGCGTTGATACCAC125250.41106666959855936No Hit
GAGTACATGGGAAGCAGTGGTATCA70370.23095218794132233No Hit
GTATCAACGCAGAGTACTTTTTTTT62430.20489335076277893No Hit
GGTATCAACGCAGAGTACTTTTTTT53610.17594638049643727No Hit
CATGTACTCTGCGTTGATACCACTG49480.16239184680029314No Hit
GCTTCCCATGTACTCTGCGTTGATA47520.15595918674110612No Hit
GCGTTGATACCACTGCTTCCCATGT42160.13836783066088035No Hit
GTATCAACGCAGAGTACATGGGAAG39880.13088494038794848No Hit
TATCAACGCAGAGTACTTTTTTTTT39360.12917831629061313No Hit
GGTATCAACGCAGAGTACATGGGAA38980.12793116791179115No Hit
ACGCAGAGTACATGGGAAGCAGTGG37730.12382870613935044No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN35880.1177570627161382No Hit
TATCAACGCAGAGTACATGGGAAGC34190.11221053439979835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGACG752.0645717E-411.406225
GTCTAGT2700.010.5649671
TAATACG909.4621915E-510.5620114
GTCTTAG3100.010.4286451
GTGTAGG3600.010.3008421
GTCTTGC5400.09.8606361
GTGTTAG4350.09.6177631
CCGTGCA800.0045199029.4993999
TAGACTG4450.08.9711855
TCCACGG850.0074109618.9451722
TATACTG7000.08.8262425
ATAGGAC2800.08.8256613
GTAGATC2054.109097E-98.8091043
TTAGACA4250.08.7229794
CTAGTAC2304.0017767E-108.6780753
CTAACAC5500.08.6405083
GTTGTAG3100.08.5882961
CAGGACT4550.08.5656754
GGGTTAG1451.6712478E-58.5248361
GTATTAG4800.08.5180041
Produced by FastQC (version 0.11.5)