FastQCFastQC Report
Wed 25 May 2016
SRR1294984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294984_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3046951
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC146630.48123517575438524No Hit
CCCATGTACTCTGCGTTGATACCAC141460.46426739386357047No Hit
GTATCAACGCAGAGTACTTTTTTTT140050.4596398169842574No Hit
GGTATCAACGCAGAGTACTTTTTTT131130.43036464977612043No Hit
TATCAACGCAGAGTACTTTTTTTTT96900.318022836599604No Hit
GAGTACATGGGAAGCAGTGGTATCA81350.2669882121504415No Hit
CATGTACTCTGCGTTGATACCACTG55690.18277287688577862No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54730.17962218624454415No Hit
GCTTCCCATGTACTCTGCGTTGATA49990.16406565120344896No Hit
ACGCAGAGTACATGGGAAGCAGTGG46140.15143006894433156No Hit
GGTATCAACGCAGAGTACATGGGAA44590.1463430163465051No Hit
GCGTTGATACCACTGCTTCCCATGT44280.14532560582693976No Hit
GTATCAACGCAGAGTACATGGGAAG43840.14388153928304065No Hit
TATCAACGCAGAGTACATGGGAAGC37850.12422254246950476No Hit
ACTCTGCGTTGATACCACTGCTTCC31850.10453072596178935No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT400.005282670314.2491764
GACAGCG658.0312026E-411.6916317
CACGACA750.002650092610.1327474
ACAGCGT1054.1103413E-59.9518058
TATTCCG800.0045197079.4994515
ACACCGT900.00111475039.499456
GTGTTAG4800.09.3130061
TAGACAG4900.09.3055845
TCTGTCG1951.6862032E-99.2558758
GGCGTGC1054.4972025E-49.0470958
TAGGACT4200.09.0470954
TACTAGG2850.08.9996272
TACACTG7250.08.9098295
TCTATAC4200.08.8209183
TATACTG7600.08.7494935
GGCTAGA2751.8189894E-128.6465061
CTGTCGC2751.8189894E-128.6357229
GTGTAGA4200.08.6053321
TTAGACT3550.08.5628844
TAGACTG4450.08.5388325
Produced by FastQC (version 0.11.5)