FastQCFastQC Report
Wed 25 May 2016
SRR1294983_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294983_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528941
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC2115198.3639357343647No Hit
CCCATGTACTCTGCGTTGATACCAC2094148.280699312479017No Hit
GAGTACATGGGAAGCAGTGGTATCA884893.49905355640958No Hit
CATGTACTCTGCGTTGATACCACTG718922.8427709464159108No Hit
GCGTTGATACCACTGCTTCCCATGT614772.4309384837368686No Hit
GTATCAACGCAGAGTACATGGGAAG521962.0639469248195192No Hit
ACGCAGAGTACATGGGAAGCAGTGG498331.9705086041944038No Hit
TATCAACGCAGAGTACATGGGAAGC497281.9663566686609137No Hit
ACTCTGCGTTGATACCACTGCTTCC447511.769554924373483No Hit
GGTATCAACGCAGAGTACATGGGAA414761.6400540779717678No Hit
GCTTCCCATGTACTCTGCGTTGATA303231.1990394398287663No Hit
GTACTCTGCGTTGATACCACTGCTT244790.9679545707076598No Hit
ATACCACTGCTTCCCATGTACTCTG241030.9530866872734477No Hit
GTGGTATCAACGCAGAGTACATGGG237740.940077289268512No Hit
GGGAAGCAGTGGTATCAACGCAGAG211800.8375047104697183No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA211410.8359625629858506No Hit
CAGTGGTATCAACGCAGAGTACATG209580.828726332484625No Hit
TACCACTGCTTCCCATGTACTCTGC195160.7717064178246943No Hit
CTGCTTCCCATGTACTCTGCGTTGA193130.7636793424599467No Hit
GATACCACTGCTTCCCATGTACTCT188340.7447386079785966No Hit
GCAGTGGTATCAACGCAGAGTACAT188020.7434732561969615No Hit
CATGGGAAGCAGTGGTATCAACGCA183460.7254419933086617No Hit
ACATGGGAAGCAGTGGTATCAACGC176150.6965366135469353No Hit
GCAGAGTACATGGGAAGCAGTGGTA175260.6930173539042627No Hit
GTTGATACCACTGCTTCCCATGTAC174970.6918706288521559No Hit
CCATGTACTCTGCGTTGATACCACT159640.6312523700632003No Hit
CCACTGCTTCCCATGTACTCTGCGT153870.6084364957505929No Hit
CTTCCCATGTACTCTGCGTTGATAC153130.6055103697555617No Hit
AAGCAGTGGTATCAACGCAGAGTAC136990.5416891892693424No Hit
ATGGGAAGCAGTGGTATCAACGCAG127870.5056266634927425No Hit
TTCCCATGTACTCTGCGTTGATACC117180.46335600553749573No Hit
GTACATGGTAAGCAGTGGTATCAAC116230.4595994924357666No Hit
TGATACCACTGCTTCCCATGTACTC115210.4555661836318048No Hit
ATCAACGCAGAGTACATGGGAAGCA106960.4229438330115254No Hit
ACCATGTACTCTGCGTTGATACCAC94540.3738323669868138No Hit
GTATCAACGCAGAGTACTTTTTTTT73240.2896073890217289No Hit
TCCATGTACTCTGCGTTGATACCAC67940.26865000013839785No Hit
GGAAGCAGTGGTATCAACGCAGAGT64620.2555219754039339No Hit
CCCCATGTACTCTGCGTTGATACCA62580.24745535779601024No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA62390.24670405517566446No Hit
AAAAAGTACTCTGCGTTGATACCAC60060.237490712515634No Hit
GTACATGGGTAAGCAGTGGTATCAA58930.23302243903673514No Hit
GGTAAGCAGTGGTATCAACGCAGAG54750.2164937813891269No Hit
GGTATCAACGCAGAGTACTTTTTTT50840.20103276430727327No Hit
TATCAACGCAGAGTACTTTTTTTTT50510.19972787028246208No Hit
CTGCGTTGATACCACTGCTTCCCAT47660.18845833097727469No Hit
GTACATGGAAAGCAGTGGTATCAAC47130.18636259208894157No Hit
CTCTGCGTTGATACCACTGCTTCCC46700.18466227563236945No Hit
TCCCATGTACTCTGCGTTGATACCA45440.1796799529921813No Hit
GAACAAAAAAAAAAAAAAAAAAAAA44620.17643748905174142No Hit
AAAGTACTCTGCGTTGATACCACTG43660.17264143370683618No Hit
TCAACGCAGAGTACATGGGAAGCAG42810.169280343036868No Hit
TGGGAAGCAGTGGTATCAACGCAGA42740.16900354733463532No Hit
GAGTACATGGTAAGCAGTGGTATCA40100.15856439513614592No Hit
GTACTTTTTTTTTTTTTTTTTTTTT38120.15073503098727886No Hit
AGTGGTATCAACGCAGAGTACATGG36260.14338017375652498No Hit
GCTTACCATGTACTCTGCGTTGATA34540.13657890793023642No Hit
TTGATACCACTGCTTCCCATGTACT31890.12610021348857092No Hit
AAACAAAAAAAAAAAAAAAAAAAAA31500.12455806600470316No Hit
GAGTACATGGAAAGCAGTGGTATCA31170.12325317197989197No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN30800.12179010898237642No Hit
GTACATGGAAGCAGTGGTATCAACG30510.12064338393026962No Hit
ACTGCTTCCCATGTACTCTGCGTTG30350.12001070803945209No Hit
AACGCAGAGTACATGGGAAGCAGTG30140.11918032093275407No Hit
ACTCTGCGTTGATACCACTGCTTAC29300.11585877250596198No Hit
GCGTTGATACCACTGCTTACCATGT28990.114632962967503No Hit
ATGTACTCTGCGTTGATACCACTGC28120.11119278781118262No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26740.10573595825288135No Hit
CTGCTTACCATGTACTCTGCGTTGA26680.10549870479382477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCT250.0060152719.0097374
GTACATA11650.016.6536251
TACATAA9600.016.3335782
GTACAAA8800.015.5626781
ACGTTCG551.1316581E-515.54140212
GTTCGGT551.1318707E-515.54109314
CGTTCGG551.1322958E-515.54047713
CAGCGTT551.9655871E-413.81430511
AGAACAA10550.013.6917742
GTCTTGG655.3987285E-513.1684211
TTCGGTT655.4715303E-513.14963515
CCCTCGG701.080195E-412.227821
TATGCAG701.0864138E-412.2203035
CTCTATG550.00304400512.1043051
TAAAGGT550.003057602812.0971054
TTAGAAC550.003061692212.0949463
ACATAAA18100.011.9183433
TCGGTTG802.8762664E-511.87166916
GAGAACA17000.011.7482971
TACATAG4000.011.6413862
Produced by FastQC (version 0.11.5)