##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294983_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2528941
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.312717853046	33.0	33.0	33.0	22.0	33.0
2	30.256644975110135	33.0	33.0	33.0	22.0	33.0
3	30.503624639720737	33.0	33.0	33.0	27.0	33.0
4	33.572349453783225	37.0	33.0	37.0	22.0	37.0
5	34.46917622831059	37.0	37.0	37.0	27.0	37.0
6	34.036121839141366	37.0	37.0	37.0	27.0	37.0
7	34.19803981192128	37.0	37.0	37.0	27.0	37.0
8	33.940289235691935	37.0	37.0	37.0	27.0	37.0
9	33.760861166788786	37.0	37.0	37.0	22.0	37.0
10	33.96009396818668	37.0	37.0	37.0	27.0	37.0
11	34.1129670482625	37.0	37.0	37.0	27.0	37.0
12	34.12706860302395	37.0	37.0	37.0	27.0	37.0
13	33.77418927527372	37.0	37.0	37.0	27.0	37.0
14	35.2725642867904	37.0	33.0	40.0	27.0	40.0
15	35.2427130565719	37.0	37.0	40.0	27.0	40.0
16	35.23516048812527	37.0	33.0	40.0	27.0	40.0
17	35.341666333852785	37.0	37.0	40.0	27.0	40.0
18	35.20888704006934	37.0	33.0	40.0	27.0	40.0
19	35.1118167644085	37.0	33.0	40.0	27.0	40.0
20	35.26323152655598	37.0	33.0	40.0	27.0	40.0
21	35.23319840201887	37.0	33.0	40.0	27.0	40.0
22	35.31275620902188	37.0	33.0	40.0	27.0	40.0
23	35.331367952040004	37.0	33.0	40.0	27.0	40.0
24	35.4674134351098	37.0	33.0	40.0	27.0	40.0
25	34.3603215733384	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7413128825088648
1101	2	0.7347622045653708
1101	3	0.5930144647188342
1101	4	0.7508238164760854
1101	5	0.6587670349031995
1101	6	0.4546872350256592
1101	7	0.48411697000295817
1101	8	0.4341623247480868
1101	9	0.562906402583792
1101	10	0.6982262666632622
1101	11	0.6663163166185058
1101	12	0.5878987446082533
1101	13	0.6231614976360191
1101	14	1.1126384073639173
1101	15	0.9204047976206837
1101	16	1.0230364504808662
1101	17	0.884651935907236
1101	18	0.9953953690697901
1101	19	0.9076877827903047
1101	20	0.8296252096709296
1101	21	0.8016883611429932
1101	22	0.8172942044444582
1101	23	0.9347949444830448
1101	24	0.8760618799460573
1101	25	1.0390539296696417
1102	1	0.5749872080045257
1102	2	0.5823491906302429
1102	3	0.47821942258732264
1102	4	0.7418832266612156
1102	5	0.49043628590646904
1102	6	0.3264022188301894
1102	7	0.48957695248682853
1102	8	0.4312333514855382
1102	9	0.4318747131189795
1102	10	0.5926790041125685
1102	11	0.34067592777697797
1102	12	0.6755446137212218
1102	13	0.45429880236893894
1102	14	0.9878449695625235
1102	15	0.913078156385339
1102	16	0.9451712089502848
1102	17	0.8706786339140749
1102	18	0.981250136992351
1102	19	0.8669474915236108
1102	20	0.832211299640413
1102	21	0.9550110715817013
1102	22	0.84589471384745
1102	23	0.947905214562816
1102	24	0.8714705651956507
1102	25	1.0717816628263392
1103	1	0.29540237571164596
1103	2	0.3170453280172332
1103	3	0.23759695759243726
1103	4	0.3733832002089912
1103	5	0.15236939998022336
1103	6	0.16956674286644358
1103	7	0.23732924795232435
1103	8	0.21518309519284884
1103	9	0.2854564916491782
1103	10	0.36827410574790775
1103	11	0.13084760273201113
1103	12	0.2803790521698275
1103	13	0.19594173550946437
1103	14	0.5586525854526698
1103	15	0.6113922677668526
1103	16	0.5657132738699318
1103	17	0.6165390073019594
1103	18	0.5659563409213888
1103	19	0.524166965653329
1103	20	0.4589887423566452
1103	21	0.5179705320738321
1103	22	0.516372558637535
1103	23	0.5073270570486201
1103	24	0.4832407776364249
1103	25	0.7131979885531834
1104	1	0.46356254368111394
1104	2	0.39968353659235945
1104	3	0.2718951884365026
1104	4	0.3477164502038832
1104	5	0.2509796748723403
1104	6	0.3430694998379593
1104	7	0.32303078183308287
1104	8	0.4883274401980984
1104	9	0.2856080031929693
1104	10	0.45386175793976236
1104	11	0.3844613443896634
1104	12	0.4084794404022105
1104	13	0.38827075848819703
1104	14	0.6804018195327615
1104	15	0.7264163168582982
1104	16	0.5664405009337159
1104	17	0.5527911984791984
1104	18	0.649100036793925
1104	19	0.6315466266529839
1104	20	0.7091765373010048
1104	21	0.6572159478929365
1104	22	0.6964501937761938
1104	23	0.7366518966646467
1104	24	0.8090216361016829
1104	25	1.0983406871991335
1105	1	0.35654356937368803
1105	2	0.27728141451920507
1105	3	0.20086845409963416
1105	4	0.30313323075826304
1105	5	0.09299794284765284
1105	6	0.14237852368557213
1105	7	0.06510049157940756
1105	8	0.2518140107207074
1105	9	0.3124921765978925
1105	10	0.37883418429348836
1105	11	0.21131057700952027
1105	12	0.220688736969187
1105	13	0.33683987573743224
1105	14	0.5570937237865792
1105	15	0.4516501036561422
1105	16	0.3367638464991387
1105	17	0.354153397368691
1105	18	0.5011129568762556
1105	19	0.4549327596290098
1105	20	0.41905871717046494
1105	21	0.4268074617358195
1105	22	0.45703992454318154
1105	23	0.5092834835979758
1105	24	0.5007535743886962
1105	25	0.4687806565911785
1106	1	0.1240209291285872
1106	2	-0.013037934525709005
1106	3	-0.14862588125136966
1106	4	0.19834813102069404
1106	5	-0.2940429708273058
1106	6	-0.1601555818155589
1106	7	-0.64491139840122
1106	8	-0.2147355796855024
1106	9	0.13443031038106312
1106	10	-0.2510074742191577
1106	11	-0.369503089150939
1106	12	-0.6729779959452884
1106	13	-0.12802328099587612
1106	14	-0.23877232084320354
1106	15	-0.08571054223918395
1106	16	-0.10951483885818192
1106	17	0.03069121782513662
1106	18	-0.17454563748381702
1106	19	-0.46729277883061116
1106	20	-0.13918868444981314
1106	21	0.04180384118939884
1106	22	0.1996051088055708
1106	23	-0.5032230401950315
1106	24	0.1424907534186559
1106	25	0.218299321275488
1107	1	-1.1154022801588006
1107	2	-1.0743879828239749
1107	3	-0.7810855660242986
1107	4	-1.01490121274648
1107	5	-1.1013277435195477
1107	6	-0.9587649035573094
1107	7	-1.0851516337390805
1107	8	-1.0445025773217722
1107	9	-0.9521166077660226
1107	10	-0.8415071583096747
1107	11	-0.885852802126287
1107	12	-0.9149088470654405
1107	13	-0.8796177561684431
1107	14	-1.2345604149971763
1107	15	-1.0950011195475895
1107	16	-1.0872613100257098
1107	17	-1.0678964402913849
1107	18	-1.470059178646764
1107	19	-1.2844109695693433
1107	20	-1.2396729760419163
1107	21	-1.2878521174988222
1107	22	-1.1296575808136566
1107	23	-0.9343058714264174
1107	24	-0.8059236398174647
1107	25	-0.9168486826119064
1108	1	-0.006251636020778051
1108	2	-0.22800998311280196
1108	3	-0.17138035814225816
1108	4	-0.27773504655040426
1108	5	-0.47713571718632863
1108	6	-0.32270251155686935
1108	7	-0.16958917700114995
1108	8	-0.01364433250450503
1108	9	-0.3170890818834806
1108	10	-0.6290480549737012
1108	11	-0.1475550370636185
1108	12	-0.1628767746192139
1108	13	-0.10236009847041316
1108	14	-0.4165681859858168
1108	15	-0.20625367792337101
1108	16	-0.9861013885074641
1108	17	-0.9919650296614861
1108	18	-0.8062619389537176
1108	19	-0.18975996688522656
1108	20	-1.0890215550540177
1108	21	-0.31704125554404783
1108	22	-0.45025231914970476
1108	23	-0.20394056524298776
1108	24	-0.6072522522938755
1108	25	-1.183672773423666
1109	1	0.2093681809564849
1109	2	0.15126033656837734
1109	3	0.20676262386825428
1109	4	0.139566848704213
1109	5	0.1606787833879011
1109	6	0.11830621162012989
1109	7	0.13062257082162176
1109	8	0.2675046352881054
1109	9	0.31540217068450005
1109	10	0.297377091203721
1109	11	0.24229695969007992
1109	12	0.24951751143349554
1109	13	0.12621481636172405
1109	14	0.42999585962076736
1109	15	0.3181086903071346
1109	16	0.26151987841413415
1109	17	0.4015448028479156
1109	18	0.4705136253177713
1109	19	0.31114711138468465
1109	20	0.41743620296657724
1109	21	0.37388549161357787
1109	22	0.4788134399327575
1109	23	0.5225077943289165
1109	24	0.524979694753398
1109	25	0.3731786450784256
1110	1	-0.014760631683738978
1110	2	-0.06542529996556468
1110	3	0.03574899485829519
1110	4	-0.9590178334851984
1110	5	-0.3611695323506652
1110	6	-0.04226814116185551
1110	7	0.09042805083834793
1110	8	0.10607163521379448
1110	9	0.06263066640487125
1110	10	-0.11453960133006547
1110	11	-0.06207906122977391
1110	12	0.10199521238883591
1110	13	0.2393770115403413
1110	14	0.22303726641010968
1110	15	0.008471612940184059
1110	16	0.1638851494782685
1110	17	0.15059448703892997
1110	18	0.24198019918571845
1110	19	0.1141535718905331
1110	20	0.07630814043905332
1110	21	0.18983262882653662
1110	22	-0.46890403575557826
1110	23	0.028975696309942123
1110	24	0.14994573781117282
1110	25	0.2464169664571898
1111	1	-0.2091984163876468
1111	2	-0.35864035630696733
1111	3	-0.2545890535713369
1111	4	-0.32080157839050116
1111	5	-0.24489046258322134
1111	6	-0.12977713181157924
1111	7	-0.20734226803002542
1111	8	-0.021955216860050086
1111	9	-0.2447296788360802
1111	10	-0.1658462424233278
1111	11	-0.22199275099242044
1111	12	-0.09244257231507191
1111	13	-0.25522859862508795
1111	14	-0.40462995967475734
1111	15	-0.3787257018524315
1111	16	-0.3146571665131006
1111	17	-0.2732846786287766
1111	18	-0.29434202570040213
1111	19	-0.3236311320173968
1111	20	-0.3281512218241929
1111	21	-0.37190316594689676
1111	22	-0.2835767761056118
1111	23	-0.16402358443808396
1111	24	-0.09603843864530859
1111	25	-0.19400499470319943
1112	1	-0.22910348461939734
1112	2	-0.5300322995886191
1112	3	-0.22807595314440476
1112	4	-0.16463473430759024
1112	5	-0.08122568926681595
1112	6	-0.203084554594831
1112	7	-0.12948167340431382
1112	8	-0.1878898520042398
1112	9	-0.1440796624185552
1112	10	0.05315132181620186
1112	11	-0.1360274765841467
1112	12	-0.10505228169127179
1112	13	-0.12747890351187152
1112	14	-0.45376731781686885
1112	15	-0.3595311838306472
1112	16	-0.30309918445761497
1112	17	-0.4403403727125834
1112	18	-0.31145184873638954
1112	19	-0.29508113982356576
1112	20	-1.023717439368511
1112	21	-1.41083295471487
1112	22	-1.7466244641108446
1112	23	-0.6879655666996953
1112	24	-0.5322373300965708
1112	25	-1.922401231177247
1113	1	-0.42303773620222174
1113	2	-0.37491558476550324
1113	3	-0.37278960566014874
1113	4	-0.7038984614994703
1113	5	-0.2083831263455025
1113	6	-0.454198131761693
1113	7	-0.39728908797816587
1113	8	-0.32912057152103813
1113	9	-0.3932485893349096
1113	10	-0.20610051058113044
1113	11	-0.3314257362678461
1113	12	-0.27246424480330234
1113	13	-0.45039479704944085
1113	14	-0.6994163100471695
1113	15	-0.5896631225150557
1113	16	-0.6933147990393564
1113	17	-0.6719904114070161
1113	18	-1.077265078543121
1113	19	-1.2050838134938573
1113	20	-1.3006827172457562
1113	21	-1.2491603764590167
1113	22	-0.6010071457587429
1113	23	-1.929181301075765
1113	24	-1.2246705176367527
1113	25	-0.6715929993110095
1114	1	-0.4426200850544255
1114	2	-0.6430125963977211
1114	3	-0.46322202507558785
1114	4	-0.7005084632393874
1114	5	-0.7468598429990152
1114	6	-0.5566628648527825
1114	7	-0.5416502700447623
1114	8	-0.33132625053774234
1114	9	-1.3424455805420337
1114	10	-0.45232880261458774
1114	11	-0.591524075110911
1114	12	-0.5644496858298353
1114	13	-0.6701778455661866
1114	14	-0.8742923095529278
1114	15	-1.4897089739094653
1114	16	-1.4383971214145888
1114	17	-1.699356780438876
1114	18	-1.7596925841684268
1114	19	-1.732478445644638
1114	20	-1.0121287760851558
1114	21	-0.711508333487302
1114	22	-0.5345754727418068
1114	23	-0.41762452225599844
1114	24	-1.1602661192650388
1114	25	-0.6884852035653068
1115	1	-0.864817484837161
1115	2	-0.6976347214654481
1115	3	-0.5424789533387369
1115	4	-0.7936798902093685
1115	5	-0.5142449724894078
1115	6	-0.577719019616687
1115	7	-0.659617445704896
1115	8	-0.3775052763771001
1115	9	-0.8346256046171305
1115	10	-0.47168934454449385
1115	11	-0.6769178043754991
1115	12	-0.7461537863366985
1115	13	-0.7456107836088393
1115	14	-0.8667837618257863
1115	15	-1.8340148397455138
1115	16	-1.2418429564494602
1115	17	-0.9573555046188673
1115	18	-2.5902942213818037
1115	19	-1.6178016578284513
1115	20	-1.1092489994337313
1115	21	-0.9916128012595067
1115	22	-0.7208541620102409
1115	23	-0.5166191867660501
1115	24	-0.6829726726559713
1115	25	-0.6933320384373687
1116	1	-1.4799549662217828
1116	2	-0.7773583057885745
1116	3	-1.5967466878925123
1116	4	-0.9244797158604499
1116	5	-1.0558660541791411
1116	6	-0.53108722273317
1116	7	-1.3914896988014007
1116	8	-2.9015071288343286
1116	9	-1.5527575286300603
1116	10	-0.7601981164743137
1116	11	-0.5097898395358627
1116	12	-0.7076929603587061
1116	13	-1.6189488388919813
1116	14	-1.4929546237632394
1116	15	-1.4346938924497437
1116	16	-0.9066582555668177
1116	17	-1.589846696366351
1116	18	-2.393989510328808
1116	19	-1.4243767913143373
1116	20	-0.8372334533220709
1116	21	-0.61377981299173
1116	22	-0.26912937903831846
1116	23	-0.2853847601197188
1116	24	-0.5050701264353492
1116	25	-0.31980056482580466
1201	1	0.5335520210717739
1201	2	0.6236167790384641
1201	3	0.547948184758976
1201	4	0.6376400396922293
1201	5	0.6192605898063803
1201	6	0.25224304250899365
1201	7	0.2444542960351157
1201	8	0.42239381272876386
1201	9	0.3863664290711881
1201	10	0.5877254107851044
1201	11	0.44877274838767534
1201	12	0.46115801677151325
1201	13	0.4092918039981157
1201	14	0.8782213414923348
1201	15	0.7990699395279535
1201	16	0.732024010638952
1201	17	0.7543167147146832
1201	18	0.6876702929042793
1201	19	0.7031308050521332
1201	20	0.7274214546010995
1201	21	0.5469870279879672
1201	22	0.809498127421719
1201	23	0.8889677590103915
1201	24	0.8431550964557957
1201	25	1.0824594252199446
1202	1	0.5677639687969247
1202	2	0.544826988364683
1202	3	0.4839426562407674
1202	4	0.5853788677967628
1202	5	0.36564363792551546
1202	6	0.19407713744262622
1202	7	0.306185951677449
1202	8	0.3197601093093354
1202	9	0.353333181236394
1202	10	0.5455314853079187
1202	11	0.35933950556601957
1202	12	0.40078021533414443
1202	13	0.44394087331123444
1202	14	0.7703758037548312
1202	15	0.654395073927553
1202	16	0.7027184470593113
1202	17	0.7442206580099224
1202	18	0.8625712756942221
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2210	22	0.5969980442630174
2210	23	0.6146429078644715
2210	24	0.6575993634950095
2210	25	0.9196142667194636
2211	1	0.034643719764535064
2211	2	-0.13307578832293032
2211	3	-0.05178141561241034
2211	4	-0.09907849232280341
2211	5	-0.11227447206987051
2211	6	-0.1885351349231641
2211	7	-0.00784282829221894
2211	8	0.06884066860187943
2211	9	0.08457416708695575
2211	10	0.13804781700468283
2211	11	0.1672770478923411
2211	12	0.023558330809777317
2211	13	-0.10453864465500118
2211	14	-0.14173757023610278
2211	15	-0.2101943757425886
2211	16	-0.20410615267746834
2211	17	-0.2581835884239183
2211	18	-0.11854745947825762
2211	19	-0.21176186789476503
2211	20	-0.11411953946431197
2211	21	-0.10299582302854304
2211	22	-0.21640503238841546
2211	23	-0.05473174176363926
2211	24	0.007159406753523001
2211	25	-0.11733309473190445
2212	1	0.19055574980944456
2212	2	0.18797899164274057
2212	3	0.18787059568868258
2212	4	0.10934229015551722
2212	5	0.18303888868911855
2212	6	0.1540412827220905
2212	7	0.18356081423723225
2212	8	0.3676110588518853
2212	9	0.07482280420028076
2212	10	0.33497852954540974
2212	11	0.24192966989632225
2212	12	0.23663850064365732
2212	13	0.15644803400182639
2212	14	0.14141753977646943
2212	15	0.22637982666356748
2212	16	0.14030070276740503
2212	17	0.09854609843671369
2212	18	0.21818559251402547
2212	19	0.14641128333381204
2212	20	0.1974980011257088
2212	21	0.250265305339866
2212	22	-0.3410878386542464
2212	23	0.20056011156005837
2212	24	0.1447122542747934
2212	25	0.09517313134646344
2213	1	-0.2633263827718757
2213	2	-0.9282877053275378
2213	3	-0.330608337811487
2213	4	-0.3173471895532103
2213	5	-0.09348849219060185
2213	6	-0.2445434178770256
2213	7	-0.20729729778841488
2213	8	0.08168242979011353
2213	9	-0.12756463549363417
2213	10	-1.6090395015200585
2213	11	-0.49991966526875586
2213	12	-0.1632690171444935
2213	13	-0.18155358534744437
2213	14	-0.15760548612973935
2213	15	-0.11807426630389983
2213	16	-0.152792409919833
2213	17	-0.2125519595044736
2213	18	-0.11224631564937937
2213	19	-0.06240802518708222
2213	20	-0.09826291169984103
2213	21	-0.09425335971317139
2213	22	0.0025951356119833235
2213	23	-1.0634478294015892
2213	24	-1.4809029561404472
2213	25	-1.4867940028668514
2214	1	-0.16802184276394172
2214	2	-0.42218090427406807
2214	3	-0.06753783341564201
2214	4	-0.08800817667896155
2214	5	-0.02073459838062064
2214	6	-0.16192473939558738
2214	7	-0.1760517964218664
2214	8	-0.031739835377337045
2214	9	-0.44590254125095186
2214	10	-0.08565473747888319
2214	11	-0.19493831226623115
2214	12	-0.26145299325811067
2214	13	-0.17378590176954134
2214	14	-0.27889863805746273
2214	15	-0.23662318709791919
2214	16	-0.33620248540884035
2214	17	-0.21161489739304074
2214	18	-0.15491650769118337
2214	19	-0.95695570733578
2214	20	-0.5252563694569616
2214	21	-0.42199331876330604
2214	22	-0.6433423459403826
2214	23	-1.7366007617079688
2214	24	-1.4101191919221776
2214	25	-1.6431910466885924
2215	1	-0.5240255230857223
2215	2	-0.19135276970608572
2215	3	-1.0843269702496343
2215	4	-0.9360180364599486
2215	5	-0.37355762898883427
2215	6	-0.28661802001079906
2215	7	-0.2229564717360475
2215	8	-0.13955321446602653
2215	9	-0.30857478435112284
2215	10	-1.3608618332884745
2215	11	-0.566125962051288
2215	12	-0.4680278383830796
2215	13	-0.39866863813851694
2215	14	-0.8267097169450324
2215	15	-1.0819365273318553
2215	16	-0.7415866656202752
2215	17	-0.5696467955873814
2215	18	-0.6023270718310485
2215	19	-1.0730434382495488
2215	20	-1.3228309006355659
2215	21	-0.6548818429356231
2215	22	-0.31538184723191875
2215	23	-0.7136424233235203
2215	24	-1.7030490264631126
2215	25	-0.8133672296602654
2216	1	-0.4817008894411181
2216	2	-0.4759980469820029
2216	3	-1.3343322821575683
2216	4	-0.6466625971554265
2216	5	-0.4370807490884019
2216	6	-0.4803818238753692
2216	7	-0.31190960092018827
2216	8	-0.36755782017404925
2216	9	-0.25275952650786593
2216	10	-0.3430847021307386
2216	11	-0.3976422984794752
2216	12	-0.3130534515110668
2216	13	-1.0654134831143338
2216	14	-0.7960826344541019
2216	15	-1.1086733468663112
2216	16	-0.8841543819037341
2216	17	-1.796063019176053
2216	18	-0.8483207984272312
2216	19	-0.6593220420628043
2216	20	-0.4637953127987444
2216	21	-0.37757737913258893
2216	22	-0.3346466370028196
2216	23	-0.3744325789551226
2216	24	-0.4601165807941996
2216	25	-0.43296276882540496
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3441.0
3	355.0
4	404.0
5	366.0
6	246.0
7	153.0
8	218.0
9	465.0
10	875.0
11	1876.0
12	3512.0
13	6462.0
14	11267.0
15	19556.0
16	26785.0
17	28698.0
18	27252.0
19	26353.0
20	25844.0
21	26257.0
22	25689.0
23	24734.0
24	24550.0
25	24121.0
26	23415.0
27	23869.0
28	25608.0
29	28497.0
30	34082.0
31	42779.0
32	57858.0
33	82513.0
34	121298.0
35	196792.0
36	368647.0
37	1214104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.523160745649875	18.267358857884492	10.244990630984548	23.96448976548109
2	10.047495276816766	26.160908721042404	33.276009398431455	30.515586603709377
3	22.51443578497664	34.907556692129965	23.4747082035833	19.103299319310093
4	12.992340521995388	24.9862975913484	25.54870071839063	36.47266116826558
5	8.956445043455856	44.987166400745245	33.73958199133856	12.316806564460345
6	31.952089042018567	28.888218858511244	20.24301764072883	18.91667445874136
7	20.79816734033761	37.59709855300078	22.26442185403858	19.340312252623036
8	29.100754291982405	35.60912822488157	20.843250406391945	14.446867076744077
9	28.599559016076416	20.81635920375613	16.54165253256655	34.0424292476009
10	17.048131962116493	36.53942261620025	28.72306653947414	17.689378882209123
11	23.31356576569509	32.97831748639271	14.374100326051941	29.33401642186026
12	25.40689145783521	37.034750515722095	24.542348649533256	13.016009376909441
13	30.54844752569888	23.87448634934625	18.18128137930433	27.39578474565054
14	20.56915340244242	34.52061167130216	21.5239239464536	23.38631097980182
15	39.34840697403518	21.829202385537165	18.510101130166458	20.312289510261202
16	14.559667674015278	29.978441128351957	36.19647775347127	19.265413444161492
17	28.045921357258695	32.63631367548765	25.21308205546176	14.104682911791894
18	29.872623629889745	24.519416317774446	27.668203298170702	17.939756754165114
19	22.070436788604837	36.65729168400206	29.221733793285992	12.050537734107106
20	24.17622693272959	29.271016009896133	25.559094291417672	20.993662765956604
21	16.81554499770789	33.26256907538533	30.910192390821567	19.011693536085215
22	20.81302829583497	31.64705271695567	15.948892918968587	31.591026068240772
23	14.053221730264937	33.5562066262869	25.765441774913562	26.625129868534607
24	19.948861064897784	43.894596091977164	18.888195799030008	17.268347044095044
25	20.833065842398305	25.660504468089325	16.897501436525392	36.608928252986985
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	27954.0
1	27954.0
2	26644.0
3	25334.0
4	25334.0
5	25334.0
6	30125.0
7	34916.0
8	34916.0
9	34916.0
10	33459.0
11	32002.0
12	32002.0
13	32002.0
14	31919.0
15	31836.0
16	31836.0
17	31836.0
18	34350.5
19	36865.0
20	36865.0
21	36865.0
22	40997.5
23	45130.0
24	45130.0
25	45130.0
26	51096.5
27	57063.0
28	57063.0
29	57063.0
30	61680.5
31	66298.0
32	66298.0
33	66298.0
34	68851.0
35	71404.0
36	71404.0
37	71404.0
38	75432.5
39	79461.0
40	79461.0
41	79461.0
42	122499.0
43	165537.0
44	165537.0
45	165537.0
46	554180.0
47	942823.0
48	942823.0
49	942823.0
50	782770.0
51	622717.0
52	622717.0
53	622717.0
54	399714.5
55	176712.0
56	176712.0
57	176712.0
58	113241.5
59	49771.0
60	49771.0
61	49771.0
62	40258.0
63	30745.0
64	30745.0
65	30745.0
66	24096.5
67	17448.0
68	17448.0
69	17448.0
70	13178.5
71	8909.0
72	8909.0
73	8909.0
74	6505.5
75	4102.0
76	4102.0
77	4102.0
78	2718.0
79	1334.0
80	1334.0
81	1334.0
82	874.0
83	414.0
84	414.0
85	414.0
86	266.5
87	119.0
88	119.0
89	119.0
90	75.5
91	32.0
92	32.0
93	32.0
94	18.5
95	5.0
96	5.0
97	5.0
98	7.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3109997425799969
2	0.18545312049589135
3	0.20474973516582634
4	0.2238881808630569
5	0.24777169574141905
6	0.19806709606906606
7	0.19608998391026122
8	0.21807547111617076
9	0.21720554176629664
10	0.22665613788538366
11	0.2020608626298518
12	0.20937617761742958
13	0.22072480140896922
14	0.20949480434695789
15	0.20028146168692748
16	0.20380072132960003
17	0.20356346787054344
18	0.2071618119995682
19	0.20573829124522874
20	0.20273308076384544
21	0.20040008841645574
22	0.20332621441148685
23	0.20700364302686383
24	0.22218786440648475
25	0.21712645727994445
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2528941.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.757799561175577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.45297573865196	14.34089594761053
2	11.251308089374259	4.220995638830314
3	4.698096404066359	2.643778519996695
4	2.3531521185858875	1.7655982310955898
5	1.3813889490205167	1.2955908510874918
6	0.8957061141535732	1.0080885453007304
7	0.5979775289693268	0.7851719841345586
8	0.4119251960449121	0.6181448209286734
9	0.29647112973315715	0.5005031424488858
>10	1.4029032128644279	4.705800254371814
>50	0.11534596511752737	1.50329155213995
>100	0.10500631866901487	3.847891266176743
>500	0.013705643903321654	1.8252437165728113
>1k	0.014549068143526061	6.241417487352416
>5k	0.0023194166605621258	2.7702190757727245
>10k+	0.0071691060417374795	51.927368966180076
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	211519	8.3639357343647	No Hit
CCCATGTACTCTGCGTTGATACCAC	209414	8.280699312479017	No Hit
GAGTACATGGGAAGCAGTGGTATCA	88489	3.49905355640958	No Hit
CATGTACTCTGCGTTGATACCACTG	71892	2.8427709464159108	No Hit
GCGTTGATACCACTGCTTCCCATGT	61477	2.4309384837368686	No Hit
GTATCAACGCAGAGTACATGGGAAG	52196	2.0639469248195192	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	49833	1.9705086041944038	No Hit
TATCAACGCAGAGTACATGGGAAGC	49728	1.9663566686609137	No Hit
ACTCTGCGTTGATACCACTGCTTCC	44751	1.769554924373483	No Hit
GGTATCAACGCAGAGTACATGGGAA	41476	1.6400540779717678	No Hit
GCTTCCCATGTACTCTGCGTTGATA	30323	1.1990394398287663	No Hit
GTACTCTGCGTTGATACCACTGCTT	24479	0.9679545707076598	No Hit
ATACCACTGCTTCCCATGTACTCTG	24103	0.9530866872734477	No Hit
GTGGTATCAACGCAGAGTACATGGG	23774	0.940077289268512	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	21180	0.8375047104697183	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	21141	0.8359625629858506	No Hit
CAGTGGTATCAACGCAGAGTACATG	20958	0.828726332484625	No Hit
TACCACTGCTTCCCATGTACTCTGC	19516	0.7717064178246943	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	19313	0.7636793424599467	No Hit
GATACCACTGCTTCCCATGTACTCT	18834	0.7447386079785966	No Hit
GCAGTGGTATCAACGCAGAGTACAT	18802	0.7434732561969615	No Hit
CATGGGAAGCAGTGGTATCAACGCA	18346	0.7254419933086617	No Hit
ACATGGGAAGCAGTGGTATCAACGC	17615	0.6965366135469353	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	17526	0.6930173539042627	No Hit
GTTGATACCACTGCTTCCCATGTAC	17497	0.6918706288521559	No Hit
CCATGTACTCTGCGTTGATACCACT	15964	0.6312523700632003	No Hit
CCACTGCTTCCCATGTACTCTGCGT	15387	0.6084364957505929	No Hit
CTTCCCATGTACTCTGCGTTGATAC	15313	0.6055103697555617	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	13699	0.5416891892693424	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	12787	0.5056266634927425	No Hit
TTCCCATGTACTCTGCGTTGATACC	11718	0.46335600553749573	No Hit
GTACATGGTAAGCAGTGGTATCAAC	11623	0.4595994924357666	No Hit
TGATACCACTGCTTCCCATGTACTC	11521	0.4555661836318048	No Hit
ATCAACGCAGAGTACATGGGAAGCA	10696	0.4229438330115254	No Hit
ACCATGTACTCTGCGTTGATACCAC	9454	0.3738323669868138	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7324	0.2896073890217289	No Hit
TCCATGTACTCTGCGTTGATACCAC	6794	0.26865000013839785	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	6462	0.2555219754039339	No Hit
CCCCATGTACTCTGCGTTGATACCA	6258	0.24745535779601024	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	6239	0.24670405517566446	No Hit
AAAAAGTACTCTGCGTTGATACCAC	6006	0.237490712515634	No Hit
GTACATGGGTAAGCAGTGGTATCAA	5893	0.23302243903673514	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	5475	0.2164937813891269	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5084	0.20103276430727327	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5051	0.19972787028246208	No Hit
CTGCGTTGATACCACTGCTTCCCAT	4766	0.18845833097727469	No Hit
GTACATGGAAAGCAGTGGTATCAAC	4713	0.18636259208894157	No Hit
CTCTGCGTTGATACCACTGCTTCCC	4670	0.18466227563236945	No Hit
TCCCATGTACTCTGCGTTGATACCA	4544	0.1796799529921813	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	4462	0.17643748905174142	No Hit
AAAGTACTCTGCGTTGATACCACTG	4366	0.17264143370683618	No Hit
TCAACGCAGAGTACATGGGAAGCAG	4281	0.169280343036868	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	4274	0.16900354733463532	No Hit
GAGTACATGGTAAGCAGTGGTATCA	4010	0.15856439513614592	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3812	0.15073503098727886	No Hit
AGTGGTATCAACGCAGAGTACATGG	3626	0.14338017375652498	No Hit
GCTTACCATGTACTCTGCGTTGATA	3454	0.13657890793023642	No Hit
TTGATACCACTGCTTCCCATGTACT	3189	0.12610021348857092	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	3150	0.12455806600470316	No Hit
GAGTACATGGAAAGCAGTGGTATCA	3117	0.12325317197989197	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3080	0.12179010898237642	No Hit
GTACATGGAAGCAGTGGTATCAACG	3051	0.12064338393026962	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	3035	0.12001070803945209	No Hit
AACGCAGAGTACATGGGAAGCAGTG	3014	0.11918032093275407	No Hit
ACTCTGCGTTGATACCACTGCTTAC	2930	0.11585877250596198	No Hit
GCGTTGATACCACTGCTTACCATGT	2899	0.114632962967503	No Hit
ATGTACTCTGCGTTGATACCACTGC	2812	0.11119278781118262	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2674	0.10573595825288135	No Hit
CTGCTTACCATGTACTCTGCGTTGA	2668	0.10549870479382477	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.954224317609624E-5	0.0	0.0	0.0
3	0.0	3.954224317609624E-5	0.0	0.0	0.0
4	0.0	3.954224317609624E-5	0.0	0.0	0.0
5	0.0	3.954224317609624E-5	0.0	3.954224317609624E-5	0.0
6	0.0	3.954224317609624E-5	0.0	3.954224317609624E-5	0.0
7	0.0	3.954224317609624E-5	0.0	3.954224317609624E-5	0.0
8	0.0	3.954224317609624E-5	0.0	7.908448635219248E-5	0.0
9	0.0	3.954224317609624E-5	0.0	7.908448635219248E-5	0.0
10	0.0	3.954224317609624E-5	0.0	3.163379454087699E-4	0.0
11	0.0	3.954224317609624E-5	0.0	3.5588018858486614E-4	0.0
12	0.0	3.954224317609624E-5	0.0	4.7450691811315487E-4	0.0
13	0.0	3.954224317609624E-5	0.0	5.535914044653474E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGGCT	25	0.00601527	19.009737	4
GTACATA	1165	0.0	16.653625	1
TACATAA	960	0.0	16.333578	2
GTACAAA	880	0.0	15.562678	1
ACGTTCG	55	1.1316581E-5	15.541402	12
GTTCGGT	55	1.1318707E-5	15.541093	14
CGTTCGG	55	1.1322958E-5	15.540477	13
CAGCGTT	55	1.9655871E-4	13.814305	11
AGAACAA	1055	0.0	13.691774	2
GTCTTGG	65	5.3987285E-5	13.168421	1
TTCGGTT	65	5.4715303E-5	13.149635	15
CCCTCGG	70	1.080195E-4	12.22782	1
TATGCAG	70	1.0864138E-4	12.220303	5
CTCTATG	55	0.003044005	12.104305	1
TAAAGGT	55	0.0030576028	12.097105	4
TTAGAAC	55	0.0030616922	12.094946	3
ACATAAA	1810	0.0	11.918343	3
TCGGTTG	80	2.8762664E-5	11.871669	16
GAGAACA	1700	0.0	11.748297	1
TACATAG	400	0.0	11.641386	2
>>END_MODULE