FastQCFastQC Report
Wed 25 May 2016
SRR1294983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294983_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528941
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC2318939.169569396834486No Hit
GTACATGGGAAGCAGTGGTATCAAC2038368.060132680042752No Hit
GAGTACATGGGAAGCAGTGGTATCA930543.6795638965084594No Hit
CATGTACTCTGCGTTGATACCACTG795683.1462972050356255No Hit
GCGTTGATACCACTGCTTCCCATGT651382.5757026360045567No Hit
ACGCAGAGTACATGGGAAGCAGTGG607402.4017958505160855No Hit
TATCAACGCAGAGTACATGGGAAGC542982.1470647199756736No Hit
GTATCAACGCAGAGTACATGGGAAG530852.099099979003069No Hit
ACTCTGCGTTGATACCACTGCTTCC497391.9667916333358508No Hit
GGTATCAACGCAGAGTACATGGGAA439471.7377629608599015No Hit
GCTTCCCATGTACTCTGCGTTGATA326811.292280049238001No Hit
GTATCAACGCAGAGTACTTTTTTTT293811.1617906467568837No Hit
CAGTGGTATCAACGCAGAGTACATG251900.9960691056058643No Hit
GTGGTATCAACGCAGAGTACATGGG250740.991482205397437No Hit
ATACCACTGCTTCCCATGTACTCTG247680.9793822789855516No Hit
GGGAAGCAGTGGTATCAACGCAGAG243030.9609951359086668No Hit
GTACTCTGCGTTGATACCACTGCTT232980.9212551815166902No Hit
TATCAACGCAGAGTACTTTTTTTTT232880.9208597590849292No Hit
CATGGGAAGCAGTGGTATCAACGCA232000.9173800416854329No Hit
GGTATCAACGCAGAGTACTTTTTTT227910.9012072642264094No Hit
GCAGTGGTATCAACGCAGAGTACAT216460.8559313957897792No Hit
ACATGGGAAGCAGTGGTATCAACGC214150.846797137616101No Hit
CTGCTTCCCATGTACTCTGCGTTGA210190.8311384093183669No Hit
TACCACTGCTTCCCATGTACTCTGC207510.8205410881471731No Hit
GATACCACTGCTTCCCATGTACTCT205230.811525456703023No Hit
GCAGAGTACATGGGAAGCAGTGGTA197210.7798125776757939No Hit
CCATGTACTCTGCGTTGATACCACT184080.7278936123855796No Hit
GTACTTTTTTTTTTTTTTTTTTTTT180800.71492375662382No Hit
CCACTGCTTCCCATGTACTCTGCGT173410.6857020389166849No Hit
CTTCCCATGTACTCTGCGTTGATAC168360.6657332061127563No Hit
GTTGATACCACTGCTTCCCATGTAC163160.6451712396611863No Hit
AAGCAGTGGTATCAACGCAGAGTAC158330.6260723362071318No Hit
ATGGGAAGCAGTGGTATCAACGCAG148920.5888630853784252No Hit
ACGCAGAGTACTTTTTTTTTTTTTT132420.5236183841378664No Hit
ATCAACGCAGAGTACATGGGAAGCA120300.47569318540843775No Hit
GTACATGGTAAGCAGTGGTATCAAC114840.45410312063428926No Hit
TTCCCATGTACTCTGCGTTGATACC112350.44425710208344127No Hit
TGATACCACTGCTTCCCATGTACTC107750.42606767022243697No Hit
ACCATGTACTCTGCGTTGATACCAC103920.4109229910859921No Hit
GAGTACTTTTTTTTTTTTTTTTTTT89680.3546148368032311No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA75610.2989789006544637No Hit
GGAAGCAGTGGTATCAACGCAGAGT75290.2977135488728286No Hit
CCCCATGTACTCTGCGTTGATACCA72190.28545545348823875No Hit
TCCATGTACTCTGCGTTGATACCAC69870.2762816530713844No Hit
AAAAAGTACTCTGCGTTGATACCAC63090.24947201219799117No Hit
GGTAAGCAGTGGTATCAACGCAGAG62140.245715499096262No Hit
GTACATGGGTAAGCAGTGGTATCAA61040.24136585234689145No Hit
CTGCGTTGATACCACTGCTTCCCAT53670.2122232191261085No Hit
CTCTGCGTTGATACCACTGCTTCCC51960.20546149554299606No Hit
TCAACGCAGAGTACATGGGAAGCAG51250.20265399627749323No Hit
TGGGAAGCAGTGGTATCAACGCAGA49540.19589227269438078No Hit
TCCCATGTACTCTGCGTTGATACCA47070.18612533862988498No Hit
GTACATGGAAAGCAGTGGTATCAAC47060.1860857963867089No Hit
AAAGTACTCTGCGTTGATACCACTG45780.1810243892601686No Hit
GCAGAGTACTTTTTTTTTTTTTTTT43960.17382770100211908No Hit
GAGTACATGGTAAGCAGTGGTATCA43090.1703875258457987No Hit
GTGGTATCAACGCAGAGTACTTTTT41350.16350717553315794No Hit
AGTGGTATCAACGCAGAGTACATGG39680.15690362092274987No Hit
AACGCAGAGTACATGGGAAGCAGTG38120.15073503098727886No Hit
ATCAACGCAGAGTACTTTTTTTTTT37640.14883700331482624No Hit
GCTTACCATGTACTCTGCGTTGATA36770.14539682815850588No Hit
GAGTACATGGAAAGCAGTGGTATCA34320.1357089785803623No Hit
ACTGCTTCCCATGTACTCTGCGTTG32730.129421761915363No Hit
ACTCTGCGTTGATACCACTGCTTAC32230.1274446497565582No Hit
GCGTTGATACCACTGCTTACCATGT30980.12250186935954614No Hit
GTACATGGAAGCAGTGGTATCAACG30790.12175056673920032No Hit
CAACGCAGAGTACATGGGAAGCAGT30530.12072246841662182No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA29870.11811268036699947No Hit
CGCAGAGTACATGGGAAGCAGTGGT29580.11696595531489268No Hit
TTGATACCACTGCTTCCCATGTACT28780.11380257586080499No Hit
ACGCAGAGTACATGGTAAGCAGTGG28770.11376303361762888No Hit
CGTTGATACCACTGCTTCCCATGTA28280.11182546370200015No Hit
CTGCTTACCATGTACTCTGCGTTGA27560.10897842219332124No Hit
ATGTACTCTGCGTTGATACCACTGC26810.10601275395511402No Hit
TATCAACGCAGAGTACATGGTAAGC25630.10134676926033465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGGT250.006035530518.99867611
GAGAGTC350.002171717416.2845787
CGCAAAA2400.015.4370352
GTACAAA4350.015.0890971
CGAGTAC953.0559022E-1014.99895411
GGACGCA1700.014.5283998
GGGACGC1800.014.2490067
GTGGCCG602.5712081E-514.2490059
GGTCGCC400.005283450214.24872515
TGATACG602.5716876E-514.24872417
GATACGA551.9624064E-413.81694618
CGTCAGA500.001500341913.2995992
TGGGACG2100.013.1178746
ACGCAAA4300.013.0522981
ACGCAGT2000.012.82410610
TGGCCGG604.0960129E-412.66578310
ATACGAC604.0960129E-412.66578319
AAAAGTA10200.012.573152
GACGCAG2050.012.5113229
AAAAAGT10550.012.1726391
Produced by FastQC (version 0.11.5)