##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294983_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2528941 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92278625717247 33.0 33.0 33.0 27.0 33.0 2 31.804455303623136 33.0 33.0 33.0 27.0 33.0 3 32.03013593436937 33.0 33.0 33.0 27.0 33.0 4 35.688389329762934 37.0 37.0 37.0 33.0 37.0 5 36.01491335701387 37.0 37.0 37.0 33.0 37.0 6 35.54252471686765 37.0 37.0 37.0 33.0 37.0 7 35.47417950833966 37.0 37.0 37.0 33.0 37.0 8 35.47395530382085 37.0 37.0 37.0 33.0 37.0 9 35.35071557620363 37.0 37.0 37.0 33.0 37.0 10 35.42604671283355 37.0 37.0 37.0 33.0 37.0 11 35.385933875088426 37.0 37.0 37.0 33.0 37.0 12 35.442340884979124 37.0 37.0 37.0 33.0 37.0 13 35.251001506164044 37.0 37.0 37.0 33.0 37.0 14 36.9206826098355 40.0 37.0 40.0 33.0 40.0 15 36.85906195518203 40.0 37.0 40.0 33.0 40.0 16 36.67825386199203 40.0 37.0 40.0 33.0 40.0 17 36.83640464526456 40.0 37.0 40.0 33.0 40.0 18 36.87286457058508 40.0 37.0 40.0 33.0 40.0 19 36.61504360916289 40.0 37.0 40.0 33.0 40.0 20 36.75038682199387 40.0 37.0 40.0 33.0 40.0 21 36.76555522647622 40.0 37.0 40.0 33.0 40.0 22 36.88203362593275 40.0 37.0 40.0 33.0 40.0 23 36.860557838241384 40.0 37.0 40.0 33.0 40.0 24 36.889545861291346 40.0 37.0 40.0 33.0 40.0 25 35.90647745439692 37.0 37.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30434384659379177 1101 2 0.24569608440947732 1101 3 0.2378770997105022 1101 4 0.3127234614004877 1101 5 0.2796527417374719 1101 6 0.39258379283061373 1101 7 0.37607857981240045 1101 8 0.38820986000132507 1101 9 0.41811048736941103 1101 10 0.471288166765639 1101 11 0.4531216770854982 1101 12 0.4272280030039681 1101 13 0.4276993454280458 1101 14 0.7943068886141802 1101 15 0.6658745721049613 1101 16 0.7254782104518256 1101 17 0.7521473013954605 1101 18 0.771022206831752 1101 19 0.8090400886104661 1101 20 0.691042577687746 1101 21 0.6964836053180434 1101 22 0.7334638755875176 1101 23 0.8405778532626158 1101 24 0.8328951138183243 1101 25 0.9144255653584139 1102 1 0.1843815840449743 1102 2 0.18006989032010523 1102 3 0.13225183741003832 1102 4 0.1066731455503458 1102 5 0.14092951047385327 1102 6 0.25299279556474374 1102 7 0.1654750473555211 1102 8 0.15641166143485208 1102 9 0.16033070741436717 1102 10 0.20265778000185009 1102 11 0.2889695505215144 1102 12 0.20049693269034918 1102 13 0.34957680992519613 1102 14 0.557501000910527 1102 15 0.6109041357384442 1102 16 0.5722694187228043 1102 17 0.6616170195706417 1102 18 0.5590354186968796 1102 19 0.6653352432705191 1102 20 0.6770148712448361 1102 21 0.624561523912007 1102 22 0.6914865593875845 1102 23 0.7570138207657209 1102 24 0.6956922013768008 1102 25 0.831364398300309 1103 1 0.08427085208245799 1103 2 0.14857403156323912 1103 3 0.11327434825545168 1103 4 0.1385721068595558 1103 5 0.1547297109229504 1103 6 0.1256264233995381 1103 7 0.2068245812084939 1103 8 0.11482552710456417 1103 9 0.2130485268103186 1103 10 0.25544672649780864 1103 11 0.2831935597386419 1103 12 0.19755691458527025 1103 13 0.19224528122159512 1103 14 0.3447005476956946 1103 15 0.45132468206511334 1103 16 0.4735043703446209 1103 17 0.5425240958353541 1103 18 0.5125725223543398 1103 19 0.4788756240650258 1103 20 0.4489652968317799 1103 21 0.5893506702692406 1103 22 0.5311685742243384 1103 23 0.6385899179453247 1103 24 0.5870330647775006 1103 25 0.6872720623922888 1104 1 0.1669965093222423 1104 2 0.16529862411199048 1104 3 0.13379282387641211 1104 4 0.09409000421381819 1104 5 0.11829557833433313 1104 6 0.254581810123895 1104 7 0.1963811980535155 1104 8 0.168230440784086 1104 9 0.2530920782295638 1104 10 0.26759117751861794 1104 11 0.34310312336271664 1104 12 0.20572549860288802 1104 13 0.2624376456067097 1104 14 0.42662371816191325 1104 15 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7 0.06275644169242867 2211 8 0.07604106361142726 2211 9 0.08774194256346135 2211 10 0.21965807353873146 2211 11 0.2081722389052345 2211 12 0.14021554469837838 2211 13 0.09327109343640672 2211 14 0.09549599375242934 2211 15 0.06042918371786499 2211 16 0.12016505317154724 2211 17 0.2252039534059591 2211 18 0.09925688375647468 2211 19 0.07004572540901677 2211 20 0.18568977096976624 2211 21 0.12054133765801822 2211 22 0.12115221787696129 2211 23 0.2961767497628571 2211 24 0.2076455640771826 2211 25 0.15263426755785048 2212 1 0.08631440623802078 2212 2 0.08650950694279658 2212 3 0.1081859590695089 2212 4 0.160810027518842 2212 5 0.06909583965160238 2212 6 0.27098273748003976 2212 7 0.11251778301318893 2212 8 0.21300509663650757 2212 9 -0.05611091931058354 2212 10 0.13069378386806108 2212 11 0.15515019528784535 2212 12 0.18910769958065288 2212 13 0.19028463099012072 2212 14 0.14100781206472845 2212 15 0.22064754634237715 2212 16 0.17796327793928413 2212 17 0.2629397110376175 2212 18 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-0.09112282420678852 2214 4 0.007199170435249869 2214 5 0.06000207364394328 2214 6 0.009269293086930475 2214 7 -0.0026883563866348936 2214 8 0.0024060211919021413 2214 9 -0.04336465665765843 2214 10 -0.13616725312986944 2214 11 -0.046849760715637956 2214 12 -0.12309347764046663 2214 13 -0.12742601019627386 2214 14 -0.27880884815959206 2214 15 -0.36111165442944326 2214 16 -0.2787619040963065 2214 17 -0.18011669564105404 2214 18 -0.11252846113705317 2214 19 -0.0799311608547697 2214 20 -0.17815901530965306 2214 21 -0.146991678401875 2214 22 -0.07175701996837347 2214 23 -0.021873581644882734 2214 24 -0.15884417442165244 2214 25 -0.33840408794139165 2215 1 -0.14184432453050633 2215 2 -0.1653701670601606 2215 3 -0.06240496874023549 2215 4 -0.10960550829814508 2215 5 -0.09681848891064959 2215 6 -0.056576605872074026 2215 7 -0.20057652309526475 2215 8 -0.2151703128470288 2215 9 -0.10537481484310973 2215 10 -0.22149336880511328 2215 11 -0.13458875627376443 2215 12 -0.24082709570139116 2215 13 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-0.9691551924025319 2216 25 -1.22115611890937 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 7.0 8 49.0 9 140.0 10 224.0 11 420.0 12 854.0 13 1628.0 14 2999.0 15 5608.0 16 7878.0 17 9265.0 18 9769.0 19 10022.0 20 10424.0 21 10957.0 22 11558.0 23 12230.0 24 13342.0 25 14220.0 26 15119.0 27 16522.0 28 18586.0 29 21984.0 30 28105.0 31 38427.0 32 53656.0 33 79311.0 34 119348.0 35 201260.0 36 401125.0 37 1413904.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.085196135144045 17.669208209750355 10.3504035229216 25.895192132183997 2 10.060841066652326 24.469054333848447 32.74230846491971 32.72779613457952 3 23.145103029291707 31.41888244921491 25.507554347847577 19.928460173645806 4 13.424077509123384 23.41209225521671 26.684568758227257 36.47926147743265 5 9.388133097342893 40.95110316040549 36.73103416641755 12.929729575834074 6 34.636157980751626 26.713078715557227 19.35387183805395 19.2968914656372 7 20.53958554193237 34.10467069022172 24.678195339472133 20.677548428373775 8 30.425213302600834 32.10470560741566 22.361333043881597 15.10874804610191 9 29.27256903185958 15.309926170677764 19.14643323035215 36.2710715671105 10 17.99353958830989 31.703467973353273 31.636839293601547 18.666153144735286 11 24.903348872116826 27.57043363210134 15.863517575143113 31.66269992063872 12 26.243475035597903 33.37681662007931 27.271850153878642 13.107858190444144 13 33.32716469705274 18.84807044266292 21.14015378884758 26.684611071436766 14 22.416102234097195 28.886320400515476 24.692588715988233 24.004988649399095 15 41.178343029750394 16.77065617584594 21.523712890099056 20.527287904304607 16 15.278727340811827 25.17421323787309 39.42207429908408 20.124985122231003 17 27.780086605421005 28.83187073166199 29.00332589807354 14.384716764843466 18 30.097222513297066 19.520186512852614 31.86037159427602 18.522219379574295 19 22.60701218415139 32.48822333142608 32.93722550269065 11.96753898173188 20 24.182137898828007 23.56100834301789 30.679758839767317 21.577094918386788 21 16.25534166277505 29.950758044572805 34.33227584194333 19.461624450708815 22 20.726976232343894 27.6907606780862 20.223326681009958 31.358936408559945 23 13.952282793469678 27.93177065024451 30.60486583119179 27.511080725094022 24 20.181325914112982 39.279662876785295 23.38988037218947 17.149130836912256 25 21.11617430970642 21.443942166708055 21.20055802388684 36.23932549969869 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9649.0 1 9649.0 2 9425.5 3 9202.0 4 9202.0 5 9202.0 6 18374.5 7 27547.0 8 27547.0 9 27547.0 10 23246.5 11 18946.0 12 18946.0 13 18946.0 14 15697.0 15 12448.0 16 12448.0 17 12448.0 18 16957.5 19 21467.0 20 21467.0 21 21467.0 22 31199.5 23 40932.0 24 40932.0 25 40932.0 26 53733.5 27 66535.0 28 66535.0 29 66535.0 30 71611.0 31 76687.0 32 76687.0 33 76687.0 34 77865.0 35 79043.0 36 79043.0 37 79043.0 38 75921.0 39 72799.0 40 72799.0 41 72799.0 42 112928.0 43 153057.0 44 153057.0 45 153057.0 46 559881.0 47 966705.0 48 966705.0 49 966705.0 50 818776.5 51 670848.0 52 670848.0 53 670848.0 54 430586.5 55 190325.0 56 190325.0 57 190325.0 58 119746.5 59 49168.0 60 49168.0 61 49168.0 62 40133.0 63 31098.0 64 31098.0 65 31098.0 66 24433.5 67 17769.0 68 17769.0 69 17769.0 70 13346.0 71 8923.0 72 8923.0 73 8923.0 74 6434.0 75 3945.0 76 3945.0 77 3945.0 78 2648.5 79 1352.0 80 1352.0 81 1352.0 82 867.0 83 382.0 84 382.0 85 382.0 86 236.0 87 90.0 88 90.0 89 90.0 90 55.5 91 21.0 92 21.0 93 21.0 94 12.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13088482491287856 2 0.002253907861037486 3 0.0 4 0.0 5 9.88556079402406E-4 6 0.0 7 0.0 8 8.699293498741173E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 7.513026203458285E-4 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 5.535914044653474E-4 25 6.722181339936361E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2528941.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.10340401668662 #Duplication Level Percentage of deduplicated Percentage of total 1 71.21971698696142 10.044404426211958 2 13.528165128931684 3.8158635683554993 3 5.849853750753805 2.4750855265623146 4 3.0501098684576275 1.7206772708056322 5 1.7414172368428076 1.2279955426408082 6 1.1107772058093857 0.9399443823633616 7 0.729381287791343 0.720073128875274 8 0.5062532411856578 0.571191519615874 9 0.3789292685990093 0.4809773311919451 >10 1.6214194330559883 3.9949124214827743 >50 0.11240856526644098 1.1120442294377884 >100 0.10592858346849178 2.93446659132214 >500 0.012395898065176481 1.2600068315329616 >1k 0.0191572970098182 6.1712601244597645 >5k 0.0030989745162941203 2.8837675273029393 >10k+ 0.010987273285042789 59.647329577838946 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 231893 9.169569396834486 No Hit GTACATGGGAAGCAGTGGTATCAAC 203836 8.060132680042752 No Hit GAGTACATGGGAAGCAGTGGTATCA 93054 3.6795638965084594 No Hit CATGTACTCTGCGTTGATACCACTG 79568 3.1462972050356255 No Hit GCGTTGATACCACTGCTTCCCATGT 65138 2.5757026360045567 No Hit ACGCAGAGTACATGGGAAGCAGTGG 60740 2.4017958505160855 No Hit TATCAACGCAGAGTACATGGGAAGC 54298 2.1470647199756736 No Hit GTATCAACGCAGAGTACATGGGAAG 53085 2.099099979003069 No Hit ACTCTGCGTTGATACCACTGCTTCC 49739 1.9667916333358508 No Hit GGTATCAACGCAGAGTACATGGGAA 43947 1.7377629608599015 No Hit GCTTCCCATGTACTCTGCGTTGATA 32681 1.292280049238001 No Hit GTATCAACGCAGAGTACTTTTTTTT 29381 1.1617906467568837 No Hit CAGTGGTATCAACGCAGAGTACATG 25190 0.9960691056058643 No Hit GTGGTATCAACGCAGAGTACATGGG 25074 0.991482205397437 No Hit ATACCACTGCTTCCCATGTACTCTG 24768 0.9793822789855516 No Hit GGGAAGCAGTGGTATCAACGCAGAG 24303 0.9609951359086668 No Hit GTACTCTGCGTTGATACCACTGCTT 23298 0.9212551815166902 No Hit TATCAACGCAGAGTACTTTTTTTTT 23288 0.9208597590849292 No Hit CATGGGAAGCAGTGGTATCAACGCA 23200 0.9173800416854329 No Hit GGTATCAACGCAGAGTACTTTTTTT 22791 0.9012072642264094 No Hit GCAGTGGTATCAACGCAGAGTACAT 21646 0.8559313957897792 No Hit ACATGGGAAGCAGTGGTATCAACGC 21415 0.846797137616101 No Hit CTGCTTCCCATGTACTCTGCGTTGA 21019 0.8311384093183669 No Hit TACCACTGCTTCCCATGTACTCTGC 20751 0.8205410881471731 No Hit GATACCACTGCTTCCCATGTACTCT 20523 0.811525456703023 No Hit GCAGAGTACATGGGAAGCAGTGGTA 19721 0.7798125776757939 No Hit CCATGTACTCTGCGTTGATACCACT 18408 0.7278936123855796 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 18080 0.71492375662382 No Hit CCACTGCTTCCCATGTACTCTGCGT 17341 0.6857020389166849 No Hit CTTCCCATGTACTCTGCGTTGATAC 16836 0.6657332061127563 No Hit GTTGATACCACTGCTTCCCATGTAC 16316 0.6451712396611863 No Hit AAGCAGTGGTATCAACGCAGAGTAC 15833 0.6260723362071318 No Hit ATGGGAAGCAGTGGTATCAACGCAG 14892 0.5888630853784252 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 13242 0.5236183841378664 No Hit ATCAACGCAGAGTACATGGGAAGCA 12030 0.47569318540843775 No Hit GTACATGGTAAGCAGTGGTATCAAC 11484 0.45410312063428926 No Hit TTCCCATGTACTCTGCGTTGATACC 11235 0.44425710208344127 No Hit TGATACCACTGCTTCCCATGTACTC 10775 0.42606767022243697 No Hit ACCATGTACTCTGCGTTGATACCAC 10392 0.4109229910859921 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 8968 0.3546148368032311 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 7561 0.2989789006544637 No Hit GGAAGCAGTGGTATCAACGCAGAGT 7529 0.2977135488728286 No Hit CCCCATGTACTCTGCGTTGATACCA 7219 0.28545545348823875 No Hit TCCATGTACTCTGCGTTGATACCAC 6987 0.2762816530713844 No Hit AAAAAGTACTCTGCGTTGATACCAC 6309 0.24947201219799117 No Hit GGTAAGCAGTGGTATCAACGCAGAG 6214 0.245715499096262 No Hit GTACATGGGTAAGCAGTGGTATCAA 6104 0.24136585234689145 No Hit CTGCGTTGATACCACTGCTTCCCAT 5367 0.2122232191261085 No Hit CTCTGCGTTGATACCACTGCTTCCC 5196 0.20546149554299606 No Hit TCAACGCAGAGTACATGGGAAGCAG 5125 0.20265399627749323 No Hit TGGGAAGCAGTGGTATCAACGCAGA 4954 0.19589227269438078 No Hit TCCCATGTACTCTGCGTTGATACCA 4707 0.18612533862988498 No Hit GTACATGGAAAGCAGTGGTATCAAC 4706 0.1860857963867089 No Hit AAAGTACTCTGCGTTGATACCACTG 4578 0.1810243892601686 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 4396 0.17382770100211908 No Hit GAGTACATGGTAAGCAGTGGTATCA 4309 0.1703875258457987 No Hit GTGGTATCAACGCAGAGTACTTTTT 4135 0.16350717553315794 No Hit AGTGGTATCAACGCAGAGTACATGG 3968 0.15690362092274987 No Hit AACGCAGAGTACATGGGAAGCAGTG 3812 0.15073503098727886 No Hit ATCAACGCAGAGTACTTTTTTTTTT 3764 0.14883700331482624 No Hit GCTTACCATGTACTCTGCGTTGATA 3677 0.14539682815850588 No Hit GAGTACATGGAAAGCAGTGGTATCA 3432 0.1357089785803623 No Hit ACTGCTTCCCATGTACTCTGCGTTG 3273 0.129421761915363 No Hit ACTCTGCGTTGATACCACTGCTTAC 3223 0.1274446497565582 No Hit GCGTTGATACCACTGCTTACCATGT 3098 0.12250186935954614 No Hit GTACATGGAAGCAGTGGTATCAACG 3079 0.12175056673920032 No Hit CAACGCAGAGTACATGGGAAGCAGT 3053 0.12072246841662182 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 2987 0.11811268036699947 No Hit CGCAGAGTACATGGGAAGCAGTGGT 2958 0.11696595531489268 No Hit TTGATACCACTGCTTCCCATGTACT 2878 0.11380257586080499 No Hit ACGCAGAGTACATGGTAAGCAGTGG 2877 0.11376303361762888 No Hit CGTTGATACCACTGCTTCCCATGTA 2828 0.11182546370200015 No Hit CTGCTTACCATGTACTCTGCGTTGA 2756 0.10897842219332124 No Hit ATGTACTCTGCGTTGATACCACTGC 2681 0.10601275395511402 No Hit TATCAACGCAGAGTACATGGTAAGC 2563 0.10134676926033465 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.954224317609624E-5 0.0 6 0.0 0.0 0.0 3.954224317609624E-5 0.0 7 0.0 0.0 0.0 3.954224317609624E-5 0.0 8 3.954224317609624E-5 0.0 0.0 3.954224317609624E-5 0.0 9 3.954224317609624E-5 0.0 0.0 3.954224317609624E-5 0.0 10 3.954224317609624E-5 0.0 0.0 2.3725345905657743E-4 0.0 11 3.954224317609624E-5 0.0 0.0 2.767957022326737E-4 0.0 12 3.954224317609624E-5 0.0 0.0 3.954224317609624E-4 0.0 13 3.954224317609624E-5 0.0 0.0 4.7450691811315487E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCCGGT 25 0.0060355305 18.998676 11 GAGAGTC 35 0.0021717174 16.284578 7 CGCAAAA 240 0.0 15.437035 2 GTACAAA 435 0.0 15.089097 1 CGAGTAC 95 3.0559022E-10 14.998954 11 GGACGCA 170 0.0 14.528399 8 GGGACGC 180 0.0 14.249006 7 GTGGCCG 60 2.5712081E-5 14.249005 9 GGTCGCC 40 0.0052834502 14.248725 15 TGATACG 60 2.5716876E-5 14.248724 17 GATACGA 55 1.9624064E-4 13.816946 18 CGTCAGA 50 0.0015003419 13.299599 2 TGGGACG 210 0.0 13.117874 6 ACGCAAA 430 0.0 13.052298 1 ACGCAGT 200 0.0 12.824106 10 TGGCCGG 60 4.0960129E-4 12.665783 10 ATACGAC 60 4.0960129E-4 12.665783 19 AAAAGTA 1020 0.0 12.57315 2 GACGCAG 205 0.0 12.511322 9 AAAAAGT 1055 0.0 12.172639 1 >>END_MODULE