FastQCFastQC Report
Wed 25 May 2016
SRR1294982_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294982_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4770542
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC184040.3857842567993322No Hit
CCCATGTACTCTGCGTTGATACCAC164410.34463589252541954No Hit
GAGTACATGGGAAGCAGTGGTATCA80740.16924701637675552No Hit
GTATCAACGCAGAGTACTTTTTTTT72830.1526660911904769No Hit
CATGTACTCTGCGTTGATACCACTG60920.12770037450671223No Hit
ATTCCATTCCATTCCATTCCATTCC56820.11910596322178907No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN55940.11726130909234213No Hit
GGTATCAACGCAGAGTACTTTTTTT55840.11705168930490498No Hit
GCGTTGATACCACTGCTTCCCATGT55240.11579397058028207No Hit
GTATCAACGCAGAGTACATGGGAAG55210.11573108464405092No Hit
GGTATCAACGCAGAGTACATGGGAA49140.10300716354661586No Hit
ACGCAGAGTACATGGGAAGCAGTGG48840.10237830418430444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCGG400.005285738514.24816310
TAGACGG1001.9214876E-611.4052425
CAGGTCG600.005883856711.0820239
AACCGTG803.7775192E-410.6863477
GACCGAG1106.0603215E-610.3625197
GGACCGA856.608006E-410.057216
TTAGACG951.6360881E-410.00565054
CGCACTG1151.0219663E-59.9176035
CTAGGAC5100.09.6904233
CCTAGAC5150.09.5963413
TAGGACT5950.09.5852454
GTACGGA1304.186717E-69.5100681
TGCCCCG800.0045012389.5043685
CCGTACA800.00452275349.4988759
TGGACAG6450.09.4306915
GTCTTAC4400.09.0777921
ACAACGT1054.5011003E-49.0465498
GCGTAGA850.00737510768.9506511
GTCTAGG5050.08.8509541
CTAGACT5750.08.761474
Produced by FastQC (version 0.11.5)