##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294982_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4770542
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.138828879401963	33.0	33.0	33.0	27.0	33.0
2	31.149189756635618	33.0	33.0	33.0	27.0	33.0
3	31.190328059159736	33.0	33.0	33.0	27.0	33.0
4	34.823960673650916	37.0	37.0	37.0	33.0	37.0
5	35.04516572750015	37.0	37.0	37.0	33.0	37.0
6	34.98527274259403	37.0	37.0	37.0	33.0	37.0
7	34.91371693195448	37.0	37.0	37.0	33.0	37.0
8	34.76109423206	37.0	37.0	37.0	27.0	37.0
9	34.77611516678817	37.0	37.0	37.0	33.0	37.0
10	34.708921124685624	37.0	37.0	37.0	27.0	37.0
11	34.84044056209965	37.0	37.0	37.0	33.0	37.0
12	34.76215532742401	37.0	37.0	37.0	27.0	37.0
13	34.686624706375085	37.0	37.0	37.0	27.0	37.0
14	36.091425460670926	40.0	37.0	40.0	27.0	40.0
15	36.24211735270332	40.0	37.0	40.0	27.0	40.0
16	36.32029756786545	40.0	37.0	40.0	27.0	40.0
17	36.256931183081505	40.0	37.0	40.0	27.0	40.0
18	36.209592117625206	40.0	37.0	40.0	27.0	40.0
19	36.30891982504294	40.0	37.0	40.0	27.0	40.0
20	36.29177690920654	40.0	37.0	40.0	27.0	40.0
21	36.297586521615365	40.0	37.0	40.0	27.0	40.0
22	36.19933101941037	40.0	37.0	40.0	27.0	40.0
23	36.10738276698958	40.0	37.0	40.0	27.0	40.0
24	36.15334546892156	40.0	37.0	40.0	27.0	40.0
25	35.01416044550074	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.40181645115165665
1101	2	0.3786472905560281
1101	3	0.3756817023729191
1101	4	0.42522064309162033
1101	5	0.35077213526646034
1101	6	0.3841068515065018
1101	7	0.371174284950186
1101	8	0.5185322822446992
1101	9	0.4049896580103649
1101	10	0.6022085175357645
1101	11	0.4787017401982254
1101	12	0.4915444837041534
1101	13	0.5266062059389682
1101	14	0.9274815555439062
1101	15	0.800842811848149
1101	16	0.7272788012010736
1101	17	0.7393506747253014
1101	18	0.7689623446975631
1101	19	0.6911318365385313
1101	20	0.5878690973856848
1101	21	0.6359747677446634
1101	22	0.7145260544265568
1101	23	0.7908083834810995
1101	24	0.7515543360832879
1101	25	0.8661604742645039
1102	1	0.39082620732715156
1102	2	0.4365400225240279
1102	3	0.48198811264719765
1102	4	0.5311983809299363
1102	5	0.40809327598901035
1102	6	0.3010697290797282
1102	7	0.4475776903270514
1102	8	0.47279948129978067
1102	9	0.46541664779636704
1102	10	0.6332629129469467
1102	11	0.44861826425415074
1102	12	0.5424714691576611
1102	13	0.5586855847965779
1102	14	0.9506067723776397
1102	15	0.9217870620956106
1102	16	0.8444941308420368
1102	17	0.8104482524217502
1102	18	0.8131238629097197
1102	19	0.7755246712474246
1102	20	0.8159056717028434
1102	21	0.8023139893706528
1102	22	0.8149894948645127
1102	23	0.8783904481109985
1102	24	0.8526702352467908
1102	25	0.8793628944958343
1103	1	0.24311566146225871
1103	2	0.2751113169218051
1103	3	0.36450352310500733
1103	4	0.3967933187421977
1103	5	0.274631112564272
1103	6	0.2680176020897136
1103	7	0.21194495676163427
1103	8	0.33625786593845675
1103	9	0.4150218308631608
1103	10	0.44961908453593225
1103	11	0.3369077720587157
1103	12	0.3699057190256525
1103	13	0.36738391358042577
1103	14	0.6578421435690487
1103	15	0.6718857937336651
1103	16	0.5096813912203899
1103	17	0.585763387306173
1103	18	0.6328926931075216
1103	19	0.5974287567298902
1103	20	0.6226325949089428
1103	21	0.6641743570432865
1103	22	0.6574511060725925
1103	23	0.6879635715675079
1103	24	0.7167631121780715
1103	25	0.7702022980176579
1104	1	0.2259448925400278
1104	2	0.23842985953886142
1104	3	0.2854053062059343
1104	4	0.276727915396215
1104	5	0.24940664606583596
1104	6	0.24151299088259748
1104	7	0.2559795180608404
1104	8	0.2946633164375143
1104	9	0.37607801370739224
1104	10	0.4181128855355851
1104	11	0.3393708205504353
1104	12	0.41105358269398096
1104	13	0.3445429537831117
1104	14	0.7111824336940344
1104	15	0.617713366082576
1104	16	0.501244815105899
1104	17	0.5433663611085819
1104	18	0.6157593044803278
1104	19	0.5458628657449154
1104	20	0.5301956804593146
1104	21	0.3627090929432484
1104	22	0.581082450475769
1104	23	0.6039986488886484
1104	24	0.5738625038615837
1104	25	0.6630937742411405
1105	1	0.19529835698654452
1105	2	0.2052427758647184
1105	3	0.19763534761124646
1105	4	0.21198195613084891
1105	5	0.11305651720137178
1105	6	0.10414012343218815
1105	7	0.12506208898754068
1105	8	0.2582561381221069
1105	9	0.26685032316336077
1105	10	0.419928594526759
1105	11	0.29774810214156133
1105	12	0.338019285853953
1105	13	0.32779323988287956
1105	14	0.6403467170338928
1105	15	0.4819115400979328
1105	16	0.39873179754668797
1105	17	0.2946568638273348
1105	18	0.35948489226691294
1105	19	0.3563988289245117
1105	20	0.3384252705473898
1105	21	0.4056664918520241
1105	22	0.36826031575746754
1105	23	0.4890849172311249
1105	24	0.4804536679241238
1105	25	0.40716883210332355
1106	1	-0.027766044228219044
1106	2	-0.21596504339051847
1106	3	-0.16439194228551202
1106	4	-0.07191483769967277
1106	5	-0.4620779457905684
1106	6	-0.5112386094260302
1106	7	-0.6700399989032064
1106	8	-0.35531793915990306
1106	9	0.02610457241934938
1106	10	-0.44075674938095233
1106	11	-0.47272413233088173
1106	12	-0.6996383269955402
1106	13	-0.3018371629742944
1106	14	-0.2635604873789177
1106	15	-0.23317970931534404
1106	16	-0.1022873483085931
1106	17	-0.31336496680178527
1106	18	-0.36365308698047727
1106	19	-0.5321794333717094
1106	20	-0.15562848182874944
1106	21	-0.034887058807584026
1106	22	0.1933089888338202
1106	23	-0.6756674553057636
1106	24	-0.008555311509837793
1106	25	0.08993522733261017
1107	1	-1.1474464940961226
1107	2	-1.2226288312544291
1107	3	-0.8505674023883465
1107	4	-0.9328022422668312
1107	5	-1.0230832839321238
1107	6	-1.0421681248052082
1107	7	-1.0238316438893307
1107	8	-1.1061742795893252
1107	9	-1.0899347568276738
1107	10	-0.9940871756681915
1107	11	-0.9554616800841842
1107	12	-1.0353734785540638
1107	13	-1.0468765994955618
1107	14	-1.3029318724527315
1107	15	-1.1045672796355746
1107	16	-1.072180016575949
1107	17	-1.1641814538685793
1107	18	-1.340838701217784
1107	19	-1.2651623955296216
1107	20	-1.4671988631848834
1107	21	-1.2804725877701912
1107	22	-1.1518046015713566
1107	23	-0.9586198238800705
1107	24	-0.9200875416686074
1107	25	-0.7845709745254226
1108	1	-0.05684091942559277
1108	2	-0.19738306764369185
1108	3	-0.10514324567906996
1108	4	-0.21659487889916562
1108	5	-0.4274586461834886
1108	6	-0.13801359845084704
1108	7	-0.1028799168157235
1108	8	0.06850893988477225
1108	9	-0.28528579637132623
1108	10	-0.48323577563454023
1108	11	-0.04200838267590257
1108	12	0.06349126829513096
1108	13	0.15259190158396763
1108	14	-0.30215037846073756
1108	15	0.07724772099918908
1108	16	-0.7124926456346685
1108	17	-0.8156397355812146
1108	18	-0.6223721377573668
1108	19	-0.14931483937269263
1108	20	-0.8541816413654004
1108	21	-0.1575750849127644
1108	22	-0.35840853205537826
1108	23	-0.1708946314276787
1108	24	-0.3861311608081479
1108	25	-1.0432392269620792
1109	1	0.10167499327014795
1109	2	0.09595902462730876
1109	3	0.11994112955690994
1109	4	0.16856522992258505
1109	5	0.11878720624817163
1109	6	0.10179678496702138
1109	7	0.12889528348335944
1109	8	0.24708754229739327
1109	9	0.1692974626656749
1109	10	0.1585660608863364
1109	11	0.15844708628448956
1109	12	0.23521692192778687
1109	13	0.2843894774438098
1109	14	0.49821805618593373
1109	15	0.39544892693095335
1109	16	0.3152794538969843
1109	17	0.36961624101630974
1109	18	0.35461027497889575
1109	19	0.2810484466227834
1109	20	0.30252128865880223
1109	21	0.3142840939789622
1109	22	0.30168673101137955
1109	23	0.3778914964743194
1109	24	0.34081315907423004
1109	25	0.2724506286924182
1110	1	-0.02826638089429423
1110	2	-0.08767176956544631
1110	3	0.09820502358700622
1110	4	-0.9897071437496479
1110	5	-0.262465958478991
1110	6	0.026316442900068182
1110	7	0.025166049239821575
1110	8	0.10793838643983378
1110	9	0.0977119474131456
1110	10	-0.12083132468411861
1110	11	0.029032603638917465
1110	12	0.08340310225303682
1110	13	0.11296703582534207
1110	14	0.19609971039542984
1110	15	0.009951086760800365
1110	16	0.05535648598754506
1110	17	0.08008383809705322
1110	18	0.14171447285216487
1110	19	0.12075286623588966
1110	20	0.13504817297117455
1110	21	0.17725911917082726
1110	22	-0.31119713702662466
1110	23	0.026914884652526894
1110	24	0.14412551427915332
1110	25	0.12371466621850402
1111	1	-0.005469753197537841
1111	2	-0.21840972049337992
1111	3	-0.06603833799330872
1111	4	-0.006399715170971376
1111	5	-0.09618729156567696
1111	6	-0.046700824786590545
1111	7	0.025605063428010055
1111	8	0.09549623877102675
1111	9	0.028206434936635105
1111	10	-0.06517271655786061
1111	11	-0.0912070031425074
1111	12	0.0588509637774024
1111	13	-0.10936740109182352
1111	14	-0.10577962384810746
1111	15	-0.03815202764143777
1111	16	-0.09656683839557445
1111	17	0.019593958251917343
1111	18	0.007213669912253806
1111	19	-0.022888682663356974
1111	20	-0.08138667489030382
1111	21	-0.18339455966432894
1111	22	-0.06029219384391382
1111	23	0.054440499273241016
1111	24	0.020249721426971234
1111	25	0.006162342896594453
1112	1	-0.14587228505719807
1112	2	-0.5406400801521194
1112	3	-0.2215579930936471
1112	4	-0.23252950249667492
1112	5	-0.14295505391423546
1112	6	-0.13760444722230147
1112	7	-0.16769958145266628
1112	8	-0.04655484199223281
1112	9	-0.1723713230560051
1112	10	0.05515300428339032
1112	11	-0.139698146899093
1112	12	-0.17442556705789514
1112	13	-0.16583881714938542
1112	14	-0.44997547758487144
1112	15	-0.26610236694062905
1112	16	-0.2330365950543083
1112	17	-0.33620986889616944
1112	18	-0.3249805818664129
1112	19	-0.17650066735221515
1112	20	-1.0558820341581239
1112	21	-1.4091404514555705
1112	22	-1.7279540211292144
1112	23	-0.6473309089659907
1112	24	-0.46959619381923545
1112	25	-1.5802881870739043
1113	1	-0.14913706832821205
1113	2	-0.11933950428543483
1113	3	-0.22949044127718565
1113	4	-0.5159384616089824
1113	5	-0.24716095799031024
1113	6	-0.23696023964486557
1113	7	-0.22431209472414082
1113	8	-0.039472165019979855
1113	9	-0.17661329092749156
1113	10	-0.017992054399620372
1113	11	-0.11414406274639077
1113	12	-0.13059153841458482
1113	13	-0.14191427559234882
1113	14	-0.40897573868674897
1113	15	-0.3438027157496606
1113	16	-0.44185920589999483
1113	17	-0.5076708565248467
1113	18	-0.8462501789328414
1113	19	-1.008376446330196
1113	20	-1.1999344651464412
1113	21	-1.0231566094784696
1113	22	-0.4930014233819051
1113	23	-1.98321434933667
1113	24	-1.0605491579747053
1113	25	-0.493388252565758
1114	1	-0.27509032335113304
1114	2	-0.39725510750899673
1114	3	-0.40998420904723076
1114	4	-0.5782383083608167
1114	5	-0.7692682547672263
1114	6	-0.40512712860957834
1114	7	-0.3919228751395991
1114	8	-0.07478885546873926
1114	9	-1.0739714676321128
1114	10	-0.2848657068624689
1114	11	-0.3898304270601187
1114	12	-0.42466949332774817
1114	13	-0.35791962035488467
1114	14	-0.7101377436978424
1114	15	-1.3881038865325124
1114	16	-1.2558706761539398
1114	17	-1.5992616185848192
1114	18	-1.8180848230411044
1114	19	-1.5560752697816866
1114	20	-0.8561933723448192
1114	21	-0.6663043978617651
1114	22	-0.4616947277706629
1114	23	-0.27728360032777033
1114	24	-0.9357706552355793
1114	25	-0.5281093064625111
1115	1	-0.7213777134421342
1115	2	-0.47502593205274124
1115	3	-0.5204176854709139
1115	4	-0.616745714398931
1115	5	-0.4124692593045225
1115	6	-0.3609370489801691
1115	7	-0.44528097397330413
1115	8	-0.15627415453932514
1115	9	-0.531044625207123
1115	10	-0.2602334528296595
1115	11	-0.5528982384885452
1115	12	-0.6091830972971408
1115	13	-0.4982462799791705
1115	14	-0.5670477885937473
1115	15	-1.5189154646116592
1115	16	-0.9044072919416948
1115	17	-0.6433092862010596
1115	18	-2.273392445550151
1115	19	-1.112821393835155
1115	20	-0.8455886723978594
1115	21	-0.8347484429198957
1115	22	-0.7575234462154157
1115	23	-0.494670777073388
1115	24	-0.39234372522710004
1115	25	-0.6505672213562903
1116	1	-1.4450213196535486
1116	2	-0.6961475100886148
1116	3	-1.607616175350465
1116	4	-0.886687238876469
1116	5	-1.0111453876287229
1116	6	-0.5634315518942756
1116	7	-1.2164906273516962
1116	8	-2.9663425545392776
1116	9	-1.555469344496153
1116	10	-0.6959534186214213
1116	11	-0.5591116734312962
1116	12	-0.6897143425344723
1116	13	-1.6883602389567542
1116	14	-1.59863806414414
1116	15	-1.5478181758334273
1116	16	-1.0026077112266947
1116	17	-1.6911058423238643
1116	18	-2.4616569047457943
1116	19	-1.32059716481443
1116	20	-0.9550962932691931
1116	21	-0.6170538203670475
1116	22	-0.4189471933847315
1116	23	-0.3840725185378915
1116	24	-0.5457433587290694
1116	25	-0.4840230833084789
1201	1	0.3791746330778629
1201	2	0.32930156841984015
1201	3	0.40499959291225807
1201	4	0.38474021432916317
1201	5	0.349792010900039
1201	6	0.18615182187321722
1201	7	0.20702515481297468
1201	8	0.3143903742795544
1201	9	0.30379938849834787
1201	10	0.5596648314965478
1201	11	0.3585436240122988
1201	12	0.42698069595267896
1201	13	0.48464990007997955
1201	14	0.8846446616643107
1201	15	0.7869503543112017
1201	16	0.6891664115494578
1201	17	0.7170574267617411
1201	18	0.6783331515361013
1201	19	0.662806801758677
1201	20	0.6620352490096337
1201	21	0.6366337137859333
1201	22	0.6738282274824599
1201	23	0.8385875655701085
1201	24	0.8044447866897215
1201	25	0.9442367067598028
1202	1	0.34905510778295934
1202	2	0.48426899219906616
1202	3	0.42042102369903134
1202	4	0.5074704224266853
1202	5	0.4444277974717181
1202	6	0.17220277919080473
1202	7	0.2677493223657521
1202	8	0.3093728255926891
1202	9	0.3474094424147438
1202	10	0.5117634224549406
1202	11	0.3297772085664761
1202	12	0.3052226917091332
1202	13	0.370714213864602
1202	14	0.7547617336001906
1202	15	0.5276969456790539
1202	16	0.5166591389655792
1202	17	0.6312136597311806
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2210	22	0.5550762041382029
2210	23	0.5949125269715836
2210	24	0.5141192002383619
2210	25	0.6759737509871826
2211	1	0.12134440780119249
2211	2	-0.09434475391965336
2211	3	-0.037846334866536324
2211	4	0.02174847899451038
2211	5	0.03942955699315576
2211	6	-0.032249694879595836
2211	7	0.0232846453094524
2211	8	0.13160417087081555
2211	9	-0.016696449649337808
2211	10	0.1656558853729635
2211	11	0.019358369019649047
2211	12	0.040692877735772015
2211	13	-0.19310731141630555
2211	14	-0.18968504047411017
2211	15	-0.17683224296647637
2211	16	-0.14141212616425491
2211	17	-0.09108443964291979
2211	18	0.01594544106589524
2211	19	-0.09944461706410834
2211	20	-0.06362023333162625
2211	21	0.011850759766126373
2211	22	-0.13468820423846495
2211	23	0.07927697183256299
2211	24	0.052401943658210826
2211	25	-0.0013317817424294276
2212	1	0.12042107265549618
2212	2	0.18187615591098805
2212	3	0.11622317416151517
2212	4	0.11221299828461895
2212	5	0.12250492770795063
2212	6	0.1321337389719517
2212	7	0.14505682551472887
2212	8	0.2581792317397813
2212	9	0.11362173939900089
2212	10	0.3453701640568241
2212	11	0.15152015254723494
2212	12	0.25914440776979575
2212	13	0.08732482464193936
2212	14	0.17589323147514335
2212	15	0.09564815819513939
2212	16	0.1339208203603519
2212	17	0.11712340224492124
2212	18	0.2070645945807641
2212	19	0.07924053205633896
2212	20	0.2365406181234775
2212	21	0.21146757103313973
2212	22	-0.45180443634019696
2212	23	0.1786158513621956
2212	24	0.15376196976814072
2212	25	0.1880536277269158
2213	1	0.03174341354336363
2213	2	-0.7353742026452963
2213	3	-0.11822823040684938
2213	4	-0.020122123206874676
2213	5	0.06086741625312442
2213	6	-0.032421879003905474
2213	7	-0.043020500484558966
2213	8	0.20681867111523644
2213	9	-0.2039088281497854
2213	10	-1.3405303397201394
2213	11	-0.37673846529015265
2213	12	-0.009607566546904422
2213	13	-0.04872030629508828
2213	14	0.09243594194452953
2213	15	0.03363993081998018
2213	16	-0.029355072376183955
2213	17	-0.016564477752105233
2213	18	0.1041661379651444
2213	19	0.019882996444195555
2213	20	0.014783756630514233
2213	21	0.10377678415787983
2213	22	0.10587057612081452
2213	23	-1.0075590720622714
2213	24	-1.4250099322687646
2213	25	-1.264902578988277
2214	1	0.011435870743945031
2214	2	-0.20858847693469684
2214	3	-0.06529283616477244
2214	4	-0.03620909282120266
2214	5	-0.12436311118160859
2214	6	-0.1147533140839272
2214	7	-0.12886250849460623
2214	8	0.04540793140382249
2214	9	-0.451387585083765
2214	10	-0.07895532661077453
2214	11	-0.14901745521135723
2214	12	-0.08687369619075014
2214	13	-0.12190462118407908
2214	14	-0.08273518790788614
2214	15	-0.18089904544716973
2214	16	-0.2691056688983835
2214	17	-0.22648842676959902
2214	18	-0.10231280416774524
2214	19	-0.9489559513905377
2214	20	-0.45749338215461677
2214	21	-0.37063774287557294
2214	22	-0.5475342901149887
2214	23	-1.935224351855517
2214	24	-1.3105649715346033
2214	25	-1.5829603772572653
2215	1	-0.41450242749048627
2215	2	-0.1726100142308553
2215	3	-1.2314571493733624
2215	4	-0.8247069740810957
2215	5	-0.30947505135052467
2215	6	-0.2499146038032336
2215	7	-0.20037537255051063
2215	8	-0.012280450697680578
2215	9	-0.2168572553924335
2215	10	-1.3105402856265584
2215	11	-0.6268981790000154
2215	12	-0.5646710827796255
2215	13	-0.5077893762917824
2215	14	-0.7592323431955137
2215	15	-1.0446044125569784
2215	16	-0.7203817530723384
2215	17	-0.5894837356457714
2215	18	-0.49715647745563984
2215	19	-1.0205439774432534
2215	20	-1.36047219279439
2215	21	-0.6274376104337236
2215	22	-0.4447734848877687
2215	23	-0.6165401570074849
2215	24	-1.7692198977311335
2215	25	-0.7751381506971029
2216	1	-0.45547671926563993
2216	2	-0.41074311909592964
2216	3	-1.4653901922848647
2216	4	-0.8128063968099468
2216	5	-0.6044770848207008
2216	6	-0.5433134557749355
2216	7	-0.44261563056458897
2216	8	-0.4392933730562376
2216	9	-0.35842859940892424
2216	10	-0.37565579658980397
2216	11	-0.46905893639431184
2216	12	-0.3395227133369829
2216	13	-1.1288717029187296
2216	14	-0.8067543050751524
2216	15	-1.1866410452777316
2216	16	-0.8345147225621758
2216	17	-1.859659541013329
2216	18	-0.8139967244601962
2216	19	-0.626505802761649
2216	20	-0.4906503227437611
2216	21	-0.3289664394126035
2216	22	-0.4451814919139565
2216	23	-0.3759126829627135
2216	24	-0.5403697953776145
2216	25	-0.3708541788259154
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6243.0
3	671.0
4	838.0
5	712.0
6	525.0
7	349.0
8	582.0
9	1173.0
10	2229.0
11	3972.0
12	7241.0
13	10817.0
14	14862.0
15	18338.0
16	21072.0
17	22400.0
18	23357.0
19	24531.0
20	25178.0
21	27512.0
22	29841.0
23	31874.0
24	33621.0
25	36323.0
26	38114.0
27	43135.0
28	48747.0
29	57279.0
30	69666.0
31	89023.0
32	119098.0
33	167256.0
34	241184.0
35	385590.0
36	699711.0
37	2467478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.05844003055672	21.09067198065835	14.057286899408231	22.793601089376704
2	15.15177987927498	20.97101658667052	40.689674869001756	23.187528665052742
3	18.330846817683547	26.1296492994243	29.634323619784276	25.905180263107873
4	11.927175616074944	17.95506941727972	38.45311418736006	31.664640779285275
5	12.963048185821322	38.44535294346328	35.11020191163043	13.481396959084973
6	30.927484915576166	38.45282025743551	17.579468506545947	13.040226320442375
7	26.111179019821083	32.84336148954465	23.266301135783678	17.779158354850594
8	23.708158094861957	37.27461986731594	21.43234079383553	17.584881243986572
9	23.57709520960499	17.598512833095807	22.3381745946382	36.486217362661
10	14.30721059512932	30.630169923193463	33.92074705107822	21.14187243059899
11	31.72544643279106	26.235085037367877	23.155419755101285	18.88404877473977
12	20.665133928993423	28.039982817093424	31.773841020303344	19.521042233609805
13	26.253695124085368	24.240791312753036	26.83560823354844	22.669905329613158
14	19.933986106119235	23.921161437853442	28.50787299604055	27.636979459986772
15	22.930511583573185	30.842842868914282	24.114987787032053	22.111657760480476
16	21.179924533628412	31.860344362581383	26.16652761428971	20.793203489500495
17	19.730578781120307	30.71014565205245	28.900049949609286	20.659225617217956
18	19.913226499631353	30.369653851243626	29.651729693509527	20.065389955615494
19	20.528174871909485	29.2456187078528	29.938899690297383	20.287306729940326
20	21.900129743912782	28.901827105996595	28.973094889072843	20.224948261017783
21	22.040214071774535	29.362741597388798	28.17593708911116	20.42110724172551
22	20.638621120467747	29.109669678803225	29.41577041583532	20.83593878489371
23	20.481311212600264	29.551100246142237	29.041381219670708	20.926207321586787
24	20.717035889189756	28.879189454862725	29.199654534694325	21.204120121253194
25	21.39318459449041	28.65869298744077	28.103259391060753	21.844863027008063
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8940.0
1	8940.0
2	7117.5
3	5295.0
4	5295.0
5	5295.0
6	7411.5
7	9528.0
8	9528.0
9	9528.0
10	14197.0
11	18866.0
12	18866.0
13	18866.0
14	31368.5
15	43871.0
16	43871.0
17	43871.0
18	70732.0
19	97593.0
20	97593.0
21	97593.0
22	141012.5
23	184432.0
24	184432.0
25	184432.0
26	240871.5
27	297311.0
28	297311.0
29	297311.0
30	355285.0
31	413259.0
32	413259.0
33	413259.0
34	461733.5
35	510208.0
36	510208.0
37	510208.0
38	540202.0
39	570196.0
40	570196.0
41	570196.0
42	564221.5
43	558247.0
44	558247.0
45	558247.0
46	569711.5
47	581176.0
48	581176.0
49	581176.0
50	538071.0
51	494966.0
52	494966.0
53	494966.0
54	438400.0
55	381834.0
56	381834.0
57	381834.0
58	328480.0
59	275126.0
60	275126.0
61	275126.0
62	224350.5
63	173575.0
64	173575.0
65	173575.0
66	131533.5
67	89492.0
68	89492.0
69	89492.0
70	63337.5
71	37183.0
72	37183.0
73	37183.0
74	25490.0
75	13797.0
76	13797.0
77	13797.0
78	8991.5
79	4186.0
80	4186.0
81	4186.0
82	2659.0
83	1132.0
84	1132.0
85	1132.0
86	687.5
87	243.0
88	243.0
89	243.0
90	149.0
91	55.0
92	55.0
93	55.0
94	32.0
95	9.0
96	9.0
97	9.0
98	15.5
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3101324755132645
2	0.18123726821816052
3	0.19974669544886095
4	0.2198492330640837
5	0.24686922366473243
6	0.19924360795901178
7	0.193856379421877
8	0.21720802374237558
9	0.21695647999745105
10	0.22892576986011234
11	0.20196866519569473
12	0.2095569015009196
13	0.22121176168242518
14	0.21022768482071846
15	0.2016332735357953
16	0.20429544483624712
17	0.2042535208787597
18	0.2069366541579552
19	0.2063916427106186
20	0.2017590454082576
21	0.19959996159765492
22	0.2031425360053428
23	0.20710434998790495
24	0.22188254500222407
25	0.21584549512403414
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4770542.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.09377570488812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.88863519732239	73.70502596815061
2	7.231059980435993	11.727879123241381
3	1.009686387440037	2.456378443060234
4	0.30824342102671193	0.999864913889903
5	0.13747030516473727	0.5573993046555864
6	0.0802789773802707	0.39060752312960756
7	0.056545244908837056	0.320982718547064
8	0.03896309122239576	0.2527731344286445
9	0.03193678473507038	0.23308870122369835
>10	0.18826205333103121	2.8573798015942464
>50	0.015947919585289235	0.8821190033236164
>100	0.0109420179037231	1.7220191792496058
>500	8.322547838068928E-4	0.47167538671488657
>1k	9.36285464139539E-4	1.6469815139519517
>5k	2.080634364750232E-4	1.0409159385260716
>10k+	5.20158591187558E-5	0.7349093463131856
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	18404	0.3857842567993322	No Hit
CCCATGTACTCTGCGTTGATACCAC	16441	0.34463589252541954	No Hit
GAGTACATGGGAAGCAGTGGTATCA	8074	0.16924701637675552	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7283	0.1526660911904769	No Hit
CATGTACTCTGCGTTGATACCACTG	6092	0.12770037450671223	No Hit
ATTCCATTCCATTCCATTCCATTCC	5682	0.11910596322178907	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5594	0.11726130909234213	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5584	0.11705168930490498	No Hit
GCGTTGATACCACTGCTTCCCATGT	5524	0.11579397058028207	No Hit
GTATCAACGCAGAGTACATGGGAAG	5521	0.11573108464405092	No Hit
GGTATCAACGCAGAGTACATGGGAA	4914	0.10300716354661586	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	4884	0.10237830418430444	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.0961978743715074E-5	0.0	0.0	0.0
6	0.0	2.0961978743715074E-5	0.0	0.0	0.0
7	0.0	2.0961978743715074E-5	0.0	0.0	0.0
8	0.0	2.0961978743715074E-5	0.0	0.0	0.0
9	0.0	2.0961978743715074E-5	0.0	2.0961978743715074E-5	0.0
10	0.0	2.0961978743715074E-5	0.0	2.0961978743715074E-5	0.0
11	0.0	2.0961978743715074E-5	0.0	2.0961978743715074E-5	0.0
12	0.0	2.0961978743715074E-5	0.0	6.288593623114523E-5	0.0
13	0.0	2.0961978743715074E-5	0.0	6.288593623114523E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGCGG	40	0.0052857385	14.248163	10
TAGACGG	100	1.9214876E-6	11.405242	5
CAGGTCG	60	0.0058838567	11.082023	9
AACCGTG	80	3.7775192E-4	10.686347	7
GACCGAG	110	6.0603215E-6	10.362519	7
GGACCGA	85	6.608006E-4	10.05721	6
TTAGACG	95	1.6360881E-4	10.0056505	4
CGCACTG	115	1.0219663E-5	9.917603	5
CTAGGAC	510	0.0	9.690423	3
CCTAGAC	515	0.0	9.596341	3
TAGGACT	595	0.0	9.585245	4
GTACGGA	130	4.186717E-6	9.510068	1
TGCCCCG	80	0.004501238	9.504368	5
CCGTACA	80	0.0045227534	9.498875	9
TGGACAG	645	0.0	9.430691	5
GTCTTAC	440	0.0	9.077792	1
ACAACGT	105	4.5011003E-4	9.046549	8
GCGTAGA	85	0.0073751076	8.950651	1
GTCTAGG	505	0.0	8.850954	1
CTAGACT	575	0.0	8.76147	4
>>END_MODULE