FastQCFastQC Report
Wed 25 May 2016
SRR1294982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294982_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4770542
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT189300.3968102576185264No Hit
CCCATGTACTCTGCGTTGATACCAC188170.3944415540204866No Hit
GTACATGGGAAGCAGTGGTATCAAC176310.3695806472304405No Hit
GGTATCAACGCAGAGTACTTTTTTT157230.3295851917874321No Hit
TATCAACGCAGAGTACTTTTTTTTT120610.2528224256279475No Hit
GAGTACATGGGAAGCAGTGGTATCA89270.18712758424514447No Hit
ACGCAGAGTACTTTTTTTTTTTTTT70900.14862042929293987No Hit
CATGTACTCTGCGTTGATACCACTG67950.14243664556354393No Hit
GCGTTGATACCACTGCTTCCCATGT59760.1252687849724413No Hit
ACGCAGAGTACATGGGAAGCAGTGG58230.12206160222465287No Hit
ATTCCATTCCATTCCATTCCATTCC57900.1213698569261103No Hit
GTATCAACGCAGAGTACATGGGAAG56020.11742900492229184No Hit
GGTATCAACGCAGAGTACATGGGAA52090.10919094727601182No Hit
GCTTCCCATGTACTCTGCGTTGATA51820.10862497384993151No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACG508.598975E-515.2249211
GTCTACG550.003032609412.1107331
CTATCGC550.003072178212.0897899
TAGGACT6050.010.2056674
TAGACAG7850.010.1646635
AATACCG951.6469818E-49.9990745
GTCTTAC4700.09.7180351
CCCGTCC1002.760092E-49.499128
CGTCCCT1002.760092E-49.4991210
ATACCGT1451.7105685E-69.1715636
CTTACAC6600.09.0673413
TAGACTG6950.09.0207465
AAACCGT950.00182643798.99916656
TAGGCCG850.0074451248.9403485
GTCCTAA4650.08.799351
TGCCCGC1752.632696E-78.6849110
TACACCG1107.1557536E-48.6355645
GTAGGAC3100.08.579853
TATACTG10200.08.4747055
CTAGGAC5500.08.4628523
Produced by FastQC (version 0.11.5)