FastQCFastQC Report
Wed 25 May 2016
SRR1294981_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294981_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3766200
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC412851.0961977590143912No Hit
CCCATGTACTCTGCGTTGATACCAC371180.9855557325686368No Hit
GAGTACATGGGAAGCAGTGGTATCA187110.4968137645371993No Hit
CATGTACTCTGCGTTGATACCACTG132890.35284902554298764No Hit
GCGTTGATACCACTGCTTCCCATGT117960.3132069459933089No Hit
GCTTCCCATGTACTCTGCGTTGATA117860.3129414263714088No Hit
GTATCAACGCAGAGTACATGGGAAG115420.30646274759704745No Hit
ACGCAGAGTACATGGGAAGCAGTGG108830.2889650045138336No Hit
TATCAACGCAGAGTACATGGGAAGC92400.24534013063565396No Hit
GGTATCAACGCAGAGTACATGGGAA88400.23471934575965162No Hit
ACTCTGCGTTGATACCACTGCTTCC82930.22019542244171844No Hit
GTGGTATCAACGCAGAGTACATGGG64260.17062290903297755No Hit
GTATCAACGCAGAGTACTTTTTTTT59970.15923211725346503No Hit
CAGTGGTATCAACGCAGAGTACATG49190.13060910201263876No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN44850.1190855504221762No Hit
GGTATCAACGCAGAGTACTTTTTTT44830.1190324464977962No Hit
ACATGGGAAGCAGTGGTATCAACGC43710.11605862673251555No Hit
CATGGGAAGCAGTGGTATCAACGCA43160.11459826881206521No Hit
GGGAAGCAGTGGTATCAACGCAGAG41920.1113058255005045No Hit
GCAGTGGTATCAACGCAGAGTACAT41880.11119961765174446No Hit
ATACCACTGCTTCCCATGTACTCTG40710.10809303807551378No Hit
GTACTCTGCGTTGATACCACTGCTT38400.10195953480962243No Hit
CTGCTTCCCATGTACTCTGCGTTGA37960.10079124847326218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGCT550.003071884612.0898327
GCACCGT951.3640569E-510.9981426
TGTCTCG909.532273E-510.55447610
CCGAGAC900.0011100459.50396253
CGTACAC900.0011100459.50396253
CTTACAC5300.09.50396253
TCCTATA3650.09.3736462
GCGCGAT1554.2797546E-79.19138416
CCAGGAC5600.09.16453553
TAGGACT5400.09.1528184
CTAGGAC3950.09.1430523
TAACCCG1054.473542E-49.0519965
TCTACAC3900.09.0165813
GTGTTAG5700.09.0089141
ATGCGTT850.00745322838.93907211
GTATTAG4850.08.8231631
GTCTTGC7250.08.7880051
ACTGATC3900.08.7684518
GTCCAGG5650.08.7520221
TAGACAG6750.08.7301465
Produced by FastQC (version 0.11.5)