FastQCFastQC Report
Wed 25 May 2016
SRR1294981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294981_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3766200
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC426861.1333970580425894No Hit
GTACATGGGAAGCAGTGGTATCAAC399761.0614412405076734No Hit
GAGTACATGGGAAGCAGTGGTATCA201680.5354999734480379No Hit
GTATCAACGCAGAGTACTTTTTTTT161370.42846901386012426No Hit
CATGTACTCTGCGTTGATACCACTG153230.4068557166374595No Hit
GGTATCAACGCAGAGTACTTTTTTT136710.36299187509956987No Hit
GCGTTGATACCACTGCTTCCCATGT132560.3519728107907174No Hit
GCTTCCCATGTACTCTGCGTTGATA132260.3511762519250173No Hit
ACGCAGAGTACATGGGAAGCAGTGG128190.3403696033136849No Hit
GTATCAACGCAGAGTACATGGGAAG117780.3127290106738888No Hit
TATCAACGCAGAGTACTTTTTTTTT106650.2831766767564123No Hit
TATCAACGCAGAGTACATGGGAAGC99180.263342361000478No Hit
ACTCTGCGTTGATACCACTGCTTCC98830.2624130423238277No Hit
GGTATCAACGCAGAGTACATGGGAA96940.25739472146991665No Hit
GTGGTATCAACGCAGAGTACATGGG66270.17595985343316872No Hit
ACGCAGAGTACTTTTTTTTTTTTTT64360.1708884286548776No Hit
CAGTGGTATCAACGCAGAGTACATG58460.15522277096277415No Hit
ACATGGGAAGCAGTGGTATCAACGC54310.14420370665392174No Hit
CATGGGAAGCAGTGGTATCAACGCA51270.13613191014815995No Hit
GGGAAGCAGTGGTATCAACGCAGAG47770.12683872338165791No Hit
GCAGTGGTATCAACGCAGAGTACAT47670.12657320375975784No Hit
ATACCACTGCTTCCCATGTACTCTG43730.11611173065689555No Hit
CTGCTTCCCATGTACTCTGCGTTGA41540.11029685093728427No Hit
CCATGTACTCTGCGTTGATACCACT40680.10801338218894377No Hit
CTTCCCATGTACTCTGCGTTGATAC40180.10668578407944347No Hit
GATACCACTGCTTCCCATGTACTCT39830.10575646540279326No Hit
GTACTCTGCGTTGATACCACTGCTT38430.10203919069619244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGA751.72804E-1017.732187
AACGCCG901.3460522E-1015.8323046
CGCCGAG1051.9985237E-812.6658438
ACACCGT1104.978956E-711.2265436
CTAGGAC3500.011.12799553
CGCAAAA1053.470932E-610.856872
GCCGAGT1201.5269252E-610.2908619
AGGCCCG750.002650205310.1328095
TACACCG750.002650205310.1328095
TGTGCGC750.00265078910.13254110
TAACCCG1054.110763E-59.9518665
ACGCAAA1502.6052294E-79.5115051
GTACTAG2303.6379788E-129.5115051
CAACGCC1401.0604617E-69.4995095
TAAGGTG3300.09.4995095
TAGACAG6650.09.4280845
AGCACCG1151.112032E-49.0864875
AAGACGG1054.497552E-49.0471515
TACACTG7700.09.0060285
TAAGACG950.00182557188.9995354
Produced by FastQC (version 0.11.5)