FastQCFastQC Report
Wed 25 May 2016
SRR1294980_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294980_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186940
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1739757.955179383064921No Hit
CCCATGTACTCTGCGTTGATACCAC1679057.67762261424639No Hit
GAGTACATGGGAAGCAGTGGTATCA778703.560682963410062No Hit
CATGTACTCTGCGTTGATACCACTG615482.8143433290350903No Hit
GCGTTGATACCACTGCTTCCCATGT527742.4131434790163424No Hit
ACGCAGAGTACATGGGAAGCAGTGG460002.1033956121338493No Hit
TATCAACGCAGAGTACATGGGAAGC454462.0780634128051068No Hit
GTATCAACGCAGAGTACATGGGAAG441922.0207230193786754No Hit
GGTATCAACGCAGAGTACATGGGAA412741.8872945759828803No Hit
ACTCTGCGTTGATACCACTGCTTCC403331.8442664179172725No Hit
GCTTCCCATGTACTCTGCGTTGATA283041.294228465344271No Hit
GTACTCTGCGTTGATACCACTGCTT230291.0530238598224002No Hit
GTGGTATCAACGCAGAGTACATGGG229411.048999972564405No Hit
ATACCACTGCTTCCCATGTACTCTG207480.9487228730555022No Hit
GGGAAGCAGTGGTATCAACGCAGAG190490.8710344133812541No Hit
CAGTGGTATCAACGCAGAGTACATG186850.8543901524504559No Hit
GATACCACTGCTTCCCATGTACTCT183850.8406723549800177No Hit
ACATGGGAAGCAGTGGTATCAACGC176820.8085269829076245No Hit
CATGGGAAGCAGTGGTATCAACGCA171710.7851610012163115No Hit
TACCACTGCTTCCCATGTACTCTGC169470.7749183791050509No Hit
CTGCTTCCCATGTACTCTGCGTTGA168510.7705286839145107No Hit
GCAGTGGTATCAACGCAGAGTACAT167430.7655902768251529No Hit
GTTGATACCACTGCTTCCCATGTAC165360.7561249965705505No Hit
GCAGAGTACATGGGAAGCAGTGGTA157630.7207788050883883No Hit
CCATGTACTCTGCGTTGATACCACT142110.649812066174655No Hit
CTTCCCATGTACTCTGCGTTGATAC142010.6493548062589737No Hit
CCACTGCTTCCCATGTACTCTGCGT136860.6258059206013882No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA132900.6076984279404098No Hit
AAGCAGTGGTATCAACGCAGAGTAC124850.5708890047280676No Hit
ATGGGAAGCAGTGGTATCAACGCAG120110.5492148847247752No Hit
TTCCCATGTACTCTGCGTTGATACC114580.5239284113876009No Hit
GTACATGGTAAGCAGTGGTATCAAC111170.5083358482628696No Hit
TGATACCACTGCTTCCCATGTACTC108870.49781887020220034No Hit
ATCAACGCAGAGTACATGGGAAGCA95130.43499135778759357No Hit
ACCATGTACTCTGCGTTGATACCAC86700.39644434689566244No Hit
GGAAGCAGTGGTATCAACGCAGAGT59970.27421877143405854No Hit
CCCCATGTACTCTGCGTTGATACCA58200.2661252709265No Hit
GTATCAACGCAGAGTACTTTTTTTT56760.25954072814068974No Hit
TCCATGTACTCTGCGTTGATACCAC55420.2534134452705607No Hit
GGTAAGCAGTGGTATCAACGCAGAG53000.24234775531107394No Hit
AAAAAGTACTCTGCGTTGATACCAC52710.24102170155559824No Hit
GTACATGGGTAAGCAGTGGTATCAA52200.23868967598562374No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA50720.23192222923354094No Hit
GTACTTTTTTTTTTTTTTTTTTTTT44040.201377266866032No Hit
CTCTGCGTTGATACCACTGCTTCCC43360.19826789943939932No Hit
TCAACGCAGAGTACATGGGAAGCAG43110.19712474965019616No Hit
GGTATCAACGCAGAGTACTTTTTTT42970.1964845857682424No Hit
CTGCGTTGATACCACTGCTTCCCAT42790.1956615179200161No Hit
TGGGAAGCAGTGGTATCAACGCAGA42550.19456409412238104No Hit
TATCAACGCAGAGTACTTTTTTTTT42200.1929636844174966No Hit
TCCCATGTACTCTGCGTTGATACCA41880.19150045268731652No Hit
GTACATGGAAAGCAGTGGTATCAAC40090.18331550019662177No Hit
GAGTACATGGTAAGCAGTGGTATCA39800.18198944644114606No Hit
AAAGTACTCTGCGTTGATACCACTG39630.18121210458448792No Hit
GAACAAAAAAAAAAAAAAAAAAAAA39030.17846854509040028No Hit
GCTTACCATGTACTCTGCGTTGATA35270.16127557226078446No Hit
AGTGGTATCAACGCAGAGTACATGG35140.16068113437039883No Hit
AACGCAGAGTACATGGGAAGCAGTG30550.13969290424062844No Hit
TTGATACCACTGCTTCCCATGTACT29880.13662926280556395No Hit
ACTCTGCGTTGATACCACTGCTTAC29330.13411433326931693No Hit
ATGTACTCTGCGTTGATACCACTGC29220.13361134736206756No Hit
ACTGCTTCCCATGTACTCTGCGTTG27690.1266152706521441No Hit
GAGTACATGGAAAGCAGTGGTATCA27540.1259293807786222No Hit
GCGTTGATACCACTGCTTACCATGT27480.12565502482921342No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN25940.11861322212772185No Hit
ACGCAGAGTACATGGTAAGCAGTGG25690.11747007233851867No Hit
GTACATGGAAGCAGTGGTATCAACG24990.11426925292874976No Hit
TATCAACGCAGAGTACATGGTAAGC24100.11019963967918645No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23610.1079590660923482No Hit
CTGCTTACCATGTACTCTGCGTTGA23560.10773043613450757No Hit
CAACGCAGAGTACATGGGAAGCAGT23040.10535268457296497No Hit
GTATCAACGCAGAGTACATGGTAAG22980.10507832862355619No Hit
CGTTGATACCACTGCTTCCCATGTA22950.10494115064885182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA7600.016.7682291
CTTGCGG350.002171434216.2847529
CAATCGT350.002174428416.28139317
GTACAAG4350.015.5226221
CTAGGAC508.691961E-515.2050283
AGCGGTG755.8664227E-815.19631411
TACAAGA3300.014.97464752
TACATGA5500.014.34110452
CTAGTAC400.005268437314.2547133
ATCGTTC400.00528850814.24654519
AAGCGGT759.684318E-713.9325110
TACATAA4450.013.8809932
CGGTGGT707.287428E-613.56751613
GTACATA5250.013.22391
AGAACAA11700.012.9957492
TACAAAA10600.012.192712
ACGGTAC550.003061537612.0949083
AATCGTT550.003074707512.08797718
TACGCTG1052.7237002E-711.7612097
TGTAAGG658.003472E-411.6961752
Produced by FastQC (version 0.11.5)