##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294980_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2186940 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.25995271932472 33.0 33.0 33.0 22.0 33.0 2 30.14996296194683 33.0 33.0 33.0 22.0 33.0 3 30.455636642980604 33.0 33.0 33.0 22.0 33.0 4 33.44974896430629 37.0 33.0 37.0 22.0 37.0 5 34.476249919979516 37.0 37.0 37.0 27.0 37.0 6 34.031752585804824 37.0 37.0 37.0 27.0 37.0 7 34.08980950551913 37.0 37.0 37.0 27.0 37.0 8 33.933785106130024 37.0 37.0 37.0 27.0 37.0 9 33.75458128709521 37.0 37.0 37.0 22.0 37.0 10 33.938151481064864 37.0 37.0 37.0 27.0 37.0 11 34.128293414542696 37.0 37.0 37.0 27.0 37.0 12 34.139868949308166 37.0 37.0 37.0 27.0 37.0 13 33.785043942677895 37.0 37.0 37.0 27.0 37.0 14 35.30669199886599 37.0 37.0 40.0 27.0 40.0 15 35.25186882127539 37.0 37.0 40.0 27.0 40.0 16 35.2513297118348 37.0 33.0 40.0 27.0 40.0 17 35.378320850137634 37.0 37.0 40.0 27.0 40.0 18 35.2375927094479 37.0 33.0 40.0 27.0 40.0 19 35.12653845098631 37.0 33.0 40.0 27.0 40.0 20 35.28167530887907 37.0 33.0 40.0 27.0 40.0 21 35.2281534930085 37.0 33.0 40.0 27.0 40.0 22 35.33264241360074 37.0 37.0 40.0 27.0 40.0 23 35.34158093043248 37.0 33.0 40.0 27.0 40.0 24 35.48850677201935 37.0 33.0 40.0 27.0 40.0 25 34.40475778942266 37.0 33.0 40.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6049318026153614 1101 2 0.6174751256921169 1101 3 0.5934404889350127 1101 4 0.7789406028572401 1101 5 0.6083610475002672 1101 6 0.48279865955159096 1101 7 0.47481659186711767 1101 8 0.49364014454510396 1101 9 0.44842333488044517 1101 10 0.6492391051496469 1101 11 0.5841743029404327 1101 12 0.5433952247928389 1101 13 0.5908884159388208 1101 14 1.1895956807545858 1101 15 1.048731070754343 1101 16 0.9562715817049039 1101 17 0.882998352601831 1101 18 0.94759555622813 1101 19 1.0227587185680775 1101 20 0.8559584205405244 1101 21 0.7341586422125204 1101 22 0.8486676279698813 1101 23 0.8913659048317157 1101 24 0.9401064696954649 1101 25 1.081518065018976 1102 1 0.42202742152605666 1102 2 0.4397552546844423 1102 3 0.3072503978388177 1102 4 0.4616973644710427 1102 5 0.4144489602464603 1102 6 0.06766302791852041 1102 7 0.2300264183322227 1102 8 0.2862423407235397 1102 9 0.24518084100986215 1102 10 0.5077275102460135 1102 11 0.2194255484351899 1102 12 0.40097509263418374 1102 13 0.34905063191395413 1102 14 0.7417144837674527 1102 15 0.5910236939167675 1102 16 0.7037050709301056 1102 17 0.7001093752838798 1102 18 0.6784497381381129 1102 19 0.6050306892666839 1102 20 0.5975781270986786 1102 21 0.7350346603946747 1102 22 0.7421919110170307 1102 23 0.7054289775703211 1102 24 0.8281082154562185 1102 25 0.9672979593890432 1103 1 0.5288593801528627 1103 2 0.40181186905171273 1103 3 0.44276165666835965 1103 4 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0.11731876218657078 2211 25 -0.03248450969877581 2212 1 0.1487121116687966 2212 2 0.22606672575893683 2212 3 0.24817834113547477 2212 4 0.24166139343918758 2212 5 0.29584856591648645 2212 6 0.2819212120621586 2212 7 0.3887084219090795 2212 8 0.4432217053365335 2212 9 0.3053787955654954 2212 10 0.45548423026692575 2212 11 0.19828731606698113 2212 12 0.338843701443011 2212 13 0.28590570489485856 2212 14 0.25379479325903276 2212 15 0.2794359552455248 2212 16 0.12777099200894781 2212 17 0.21545172642887422 2212 18 0.14792940326891113 2212 19 0.22811911134703422 2212 20 0.12878781817143903 2212 21 0.21546835756333138 2212 22 -0.28266312552213435 2212 23 0.24606695598517092 2212 24 0.1540461536586193 2212 25 0.11273339133624916 2213 1 -0.10706022062777265 2213 2 -0.9430083095913382 2213 3 -0.31271336487921886 2213 4 -0.21255309714798898 2213 5 -0.11584333075336417 2213 6 -0.060550184142314833 2213 7 -0.016348975630336327 2213 8 0.08441011071110438 2213 9 -0.29770044426994957 2213 10 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-0.5846742396430571 2216 7 -0.3610900504122725 2216 8 -0.48610288761531706 2216 9 -0.29417165721371674 2216 10 -0.525787967487652 2216 11 -0.4138066103808029 2216 12 -0.33759358685296803 2216 13 -1.2381777689913918 2216 14 -0.7443628583620878 2216 15 -1.2225586634267884 2216 16 -0.9200520981206282 2216 17 -1.8504358849888902 2216 18 -0.9729885367039373 2216 19 -0.7098392405619407 2216 20 -0.5716633061741945 2216 21 -0.37531972283805004 2216 22 -0.488018330689421 2216 23 -0.5388760775989283 2216 24 -0.6115431146065404 2216 25 -0.5802446046622975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2910.0 3 297.0 4 355.0 5 340.0 6 240.0 7 159.0 8 255.0 9 441.0 10 867.0 11 1630.0 12 3305.0 13 5766.0 14 10696.0 15 18549.0 16 24938.0 17 25503.0 18 23175.0 19 22168.0 20 21554.0 21 22142.0 22 21780.0 23 20008.0 24 20387.0 25 19754.0 26 19459.0 27 20258.0 28 21724.0 29 24508.0 30 29160.0 31 37206.0 32 49716.0 33 70932.0 34 104760.0 35 169522.0 36 318548.0 37 1053928.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.60159280677379 18.608509617435555 10.539019500002523 23.250878075788133 2 10.598752608276396 26.258489066426016 32.859403881435945 30.283354443861647 3 23.301941515366167 34.13564674262161 24.363007566475847 18.199404175536372 4 13.715268787243726 24.642645843711435 25.37295854091442 36.269126828130425 5 9.561314685353151 44.437670435714935 33.549770552100554 12.451244326831354 6 31.77094620629998 29.121236848700548 19.40607877194823 19.70173817305124 7 20.964861481960675 37.217754719436144 22.13319796433369 19.684185834269492 8 29.24495964600667 35.074888139793636 21.165270819929354 14.514881394270338 9 28.215550674581113 20.639831287127624 17.371706774314912 33.77291126397635 10 18.185509650837446 36.210611223524076 28.203177454873767 17.40070167076471 11 23.589998061853006 32.19949443229475 14.577797815987834 29.632709689864406 12 25.205510711300754 37.66495843047552 24.468971319227556 12.660559538996171 13 30.559962605001584 23.48213426454212 18.833010251545524 27.124892878910778 14 20.887951468807234 34.45122663698611 21.993594974955887 22.667226919250773 15 39.17226489942425 21.531669151545863 18.68044209162377 20.615623857406113 16 14.774211728481326 30.00029782837296 35.72799563795983 19.49749480518588 17 28.120353599524208 33.18021928375615 24.850112418549816 13.849314698169826 18 28.83633316119892 24.53781450996906 28.08271432911337 18.54313799971866 19 22.66411966100204 36.23686786117506 29.018269526839784 12.080742950983124 20 24.634892852724615 28.58644404836633 25.255233652995862 21.52342944591319 21 17.098170452051512 33.096028014795465 30.86108475318674 18.944716779966285 22 21.452750998863678 31.849501484549688 16.197170191708516 30.50057732487812 23 14.447253795602444 33.25192426298826 26.295297732673383 26.00552420873591 24 20.44334354541934 43.019034808930115 18.98930136958051 17.548320276070033 25 21.182792890264874 25.82931558236653 16.954657039645017 36.033234487723576 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 18684.0 1 18684.0 2 18711.0 3 18738.0 4 18738.0 5 18738.0 6 23228.5 7 27719.0 8 27719.0 9 27719.0 10 26875.5 11 26032.0 12 26032.0 13 26032.0 14 25661.5 15 25291.0 16 25291.0 17 25291.0 18 27584.5 19 29878.0 20 29878.0 21 29878.0 22 34122.5 23 38367.0 24 38367.0 25 38367.0 26 43973.5 27 49580.0 28 49580.0 29 49580.0 30 53918.0 31 58256.0 32 58256.0 33 58256.0 34 60948.5 35 63641.0 36 63641.0 37 63641.0 38 68047.5 39 72454.0 40 72454.0 41 72454.0 42 111152.0 43 149850.0 44 149850.0 45 149850.0 46 491672.5 47 833495.0 48 833495.0 49 833495.0 50 687504.5 51 541514.0 52 541514.0 53 541514.0 54 347804.0 55 154094.0 56 154094.0 57 154094.0 58 96003.5 59 37913.0 60 37913.0 61 37913.0 62 29852.5 63 21792.0 64 21792.0 65 21792.0 66 16557.0 67 11322.0 68 11322.0 69 11322.0 70 8212.0 71 5102.0 72 5102.0 73 5102.0 74 3605.0 75 2108.0 76 2108.0 77 2108.0 78 1436.0 79 764.0 80 764.0 81 764.0 82 493.5 83 223.0 84 223.0 85 223.0 86 154.0 87 85.0 88 85.0 89 85.0 90 52.5 91 20.0 92 20.0 93 20.0 94 12.5 95 5.0 96 5.0 97 5.0 98 9.0 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31500635591282794 2 0.1822180763989867 3 0.20201743074798575 4 0.22227404501266612 5 0.24687462847631853 6 0.19941104922860253 7 0.19497562804649418 8 0.21728991193174027 9 0.21815870577153465 10 0.2288585877984764 11 0.20316058053718894 12 0.20905923344947736 13 0.22085653927405416 14 0.2099280272892718 15 0.20146871884916825 16 0.20462381226736903 17 0.20444090830109649 18 0.20745882374459287 19 0.20613276998911723 20 0.2025661426468033 21 0.20023411707682878 22 0.20393792239384712 23 0.20787035766870604 24 0.22337146881030115 25 0.21715273395703588 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2186940.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.670913522211613 #Duplication Level Percentage of deduplicated Percentage of total 1 75.1351637397874 13.27706980922993 2 11.911886877404584 4.209878457939674 3 4.922790372097985 2.609706088599583 4 2.555175173803152 1.8060911812151013 5 1.462642678475745 1.2923116142620428 6 0.9160230176225344 0.9712178117257879 7 0.6206497321475659 0.7677213421054406 8 0.415383416586031 0.5872163546442045 9 0.29809090600411264 0.4740784759580747 >10 1.4786896143562427 4.738047364631717 >50 0.12773313273597303 1.5919391241345386 >100 0.11319383965961356 4.030847698159043 >500 0.01375980160920665 1.7124060592403665 >1k 0.01765408508351042 7.08189964493269 >5k 0.00259618898286918 2.8480919617344793 >10k+ 0.008567423643468294 52.00147701148736 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 173975 7.955179383064921 No Hit CCCATGTACTCTGCGTTGATACCAC 167905 7.67762261424639 No Hit GAGTACATGGGAAGCAGTGGTATCA 77870 3.560682963410062 No Hit CATGTACTCTGCGTTGATACCACTG 61548 2.8143433290350903 No Hit GCGTTGATACCACTGCTTCCCATGT 52774 2.4131434790163424 No Hit ACGCAGAGTACATGGGAAGCAGTGG 46000 2.1033956121338493 No Hit TATCAACGCAGAGTACATGGGAAGC 45446 2.0780634128051068 No Hit GTATCAACGCAGAGTACATGGGAAG 44192 2.0207230193786754 No Hit GGTATCAACGCAGAGTACATGGGAA 41274 1.8872945759828803 No Hit ACTCTGCGTTGATACCACTGCTTCC 40333 1.8442664179172725 No Hit GCTTCCCATGTACTCTGCGTTGATA 28304 1.294228465344271 No Hit GTACTCTGCGTTGATACCACTGCTT 23029 1.0530238598224002 No Hit GTGGTATCAACGCAGAGTACATGGG 22941 1.048999972564405 No Hit ATACCACTGCTTCCCATGTACTCTG 20748 0.9487228730555022 No Hit GGGAAGCAGTGGTATCAACGCAGAG 19049 0.8710344133812541 No Hit CAGTGGTATCAACGCAGAGTACATG 18685 0.8543901524504559 No Hit GATACCACTGCTTCCCATGTACTCT 18385 0.8406723549800177 No Hit ACATGGGAAGCAGTGGTATCAACGC 17682 0.8085269829076245 No Hit CATGGGAAGCAGTGGTATCAACGCA 17171 0.7851610012163115 No Hit TACCACTGCTTCCCATGTACTCTGC 16947 0.7749183791050509 No Hit CTGCTTCCCATGTACTCTGCGTTGA 16851 0.7705286839145107 No Hit GCAGTGGTATCAACGCAGAGTACAT 16743 0.7655902768251529 No Hit GTTGATACCACTGCTTCCCATGTAC 16536 0.7561249965705505 No Hit GCAGAGTACATGGGAAGCAGTGGTA 15763 0.7207788050883883 No Hit CCATGTACTCTGCGTTGATACCACT 14211 0.649812066174655 No Hit CTTCCCATGTACTCTGCGTTGATAC 14201 0.6493548062589737 No Hit CCACTGCTTCCCATGTACTCTGCGT 13686 0.6258059206013882 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 13290 0.6076984279404098 No Hit AAGCAGTGGTATCAACGCAGAGTAC 12485 0.5708890047280676 No Hit ATGGGAAGCAGTGGTATCAACGCAG 12011 0.5492148847247752 No Hit TTCCCATGTACTCTGCGTTGATACC 11458 0.5239284113876009 No Hit GTACATGGTAAGCAGTGGTATCAAC 11117 0.5083358482628696 No Hit TGATACCACTGCTTCCCATGTACTC 10887 0.49781887020220034 No Hit ATCAACGCAGAGTACATGGGAAGCA 9513 0.43499135778759357 No Hit ACCATGTACTCTGCGTTGATACCAC 8670 0.39644434689566244 No Hit GGAAGCAGTGGTATCAACGCAGAGT 5997 0.27421877143405854 No Hit CCCCATGTACTCTGCGTTGATACCA 5820 0.2661252709265 No Hit GTATCAACGCAGAGTACTTTTTTTT 5676 0.25954072814068974 No Hit TCCATGTACTCTGCGTTGATACCAC 5542 0.2534134452705607 No Hit GGTAAGCAGTGGTATCAACGCAGAG 5300 0.24234775531107394 No Hit AAAAAGTACTCTGCGTTGATACCAC 5271 0.24102170155559824 No Hit GTACATGGGTAAGCAGTGGTATCAA 5220 0.23868967598562374 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 5072 0.23192222923354094 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 4404 0.201377266866032 No Hit CTCTGCGTTGATACCACTGCTTCCC 4336 0.19826789943939932 No Hit TCAACGCAGAGTACATGGGAAGCAG 4311 0.19712474965019616 No Hit GGTATCAACGCAGAGTACTTTTTTT 4297 0.1964845857682424 No Hit CTGCGTTGATACCACTGCTTCCCAT 4279 0.1956615179200161 No Hit TGGGAAGCAGTGGTATCAACGCAGA 4255 0.19456409412238104 No Hit TATCAACGCAGAGTACTTTTTTTTT 4220 0.1929636844174966 No Hit TCCCATGTACTCTGCGTTGATACCA 4188 0.19150045268731652 No Hit GTACATGGAAAGCAGTGGTATCAAC 4009 0.18331550019662177 No Hit GAGTACATGGTAAGCAGTGGTATCA 3980 0.18198944644114606 No Hit AAAGTACTCTGCGTTGATACCACTG 3963 0.18121210458448792 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 3903 0.17846854509040028 No Hit GCTTACCATGTACTCTGCGTTGATA 3527 0.16127557226078446 No Hit AGTGGTATCAACGCAGAGTACATGG 3514 0.16068113437039883 No Hit AACGCAGAGTACATGGGAAGCAGTG 3055 0.13969290424062844 No Hit TTGATACCACTGCTTCCCATGTACT 2988 0.13662926280556395 No Hit ACTCTGCGTTGATACCACTGCTTAC 2933 0.13411433326931693 No Hit ATGTACTCTGCGTTGATACCACTGC 2922 0.13361134736206756 No Hit ACTGCTTCCCATGTACTCTGCGTTG 2769 0.1266152706521441 No Hit GAGTACATGGAAAGCAGTGGTATCA 2754 0.1259293807786222 No Hit GCGTTGATACCACTGCTTACCATGT 2748 0.12565502482921342 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 2594 0.11861322212772185 No Hit ACGCAGAGTACATGGTAAGCAGTGG 2569 0.11747007233851867 No Hit GTACATGGAAGCAGTGGTATCAACG 2499 0.11426925292874976 No Hit TATCAACGCAGAGTACATGGTAAGC 2410 0.11019963967918645 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2361 0.1079590660923482 No Hit CTGCTTACCATGTACTCTGCGTTGA 2356 0.10773043613450757 No Hit CAACGCAGAGTACATGGGAAGCAGT 2304 0.10535268457296497 No Hit GTATCAACGCAGAGTACATGGTAAG 2298 0.10507832862355619 No Hit CGTTGATACCACTGCTTCCCATGTA 2295 0.10494115064885182 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.5725991568127154E-5 0.0 9 0.0 0.0 0.0 4.5725991568127154E-5 0.0 10 0.0 0.0 0.0 9.145198313625431E-5 0.0 11 0.0 0.0 0.0 9.145198313625431E-5 0.0 12 0.0 0.0 0.0 1.3717797470438146E-4 0.0 13 0.0 0.0 0.0 1.3717797470438146E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAA 760 0.0 16.768229 1 CTTGCGG 35 0.0021714342 16.284752 9 CAATCGT 35 0.0021744284 16.281393 17 GTACAAG 435 0.0 15.522622 1 CTAGGAC 50 8.691961E-5 15.205028 3 AGCGGTG 75 5.8664227E-8 15.196314 11 TACAAGA 330 0.0 14.9746475 2 TACATGA 550 0.0 14.3411045 2 CTAGTAC 40 0.0052684373 14.254713 3 ATCGTTC 40 0.005288508 14.246545 19 AAGCGGT 75 9.684318E-7 13.93251 10 TACATAA 445 0.0 13.880993 2 CGGTGGT 70 7.287428E-6 13.567516 13 GTACATA 525 0.0 13.2239 1 AGAACAA 1170 0.0 12.995749 2 TACAAAA 1060 0.0 12.19271 2 ACGGTAC 55 0.0030615376 12.094908 3 AATCGTT 55 0.0030747075 12.087977 18 TACGCTG 105 2.7237002E-7 11.761209 7 TGTAAGG 65 8.003472E-4 11.696175 2 >>END_MODULE