FastQCFastQC Report
Wed 25 May 2016
SRR1294980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294980_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186940
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1926598.80952380952381No Hit
GTACATGGGAAGCAGTGGTATCAAC1661897.599156812715484No Hit
GAGTACATGGGAAGCAGTGGTATCA817643.7387399745763488No Hit
CATGTACTCTGCGTTGATACCACTG689953.154864788242933No Hit
GCGTTGATACCACTGCTTCCCATGT580102.6525647708670563No Hit
ACGCAGAGTACATGGGAAGCAGTGG551572.5221085169231894No Hit
TATCAACGCAGAGTACATGGGAAGC476402.1783862383055776No Hit
ACTCTGCGTTGATACCACTGCTTCC462542.1150100139921535No Hit
GTATCAACGCAGAGTACATGGGAAG441692.0196713215726083No Hit
GGTATCAACGCAGAGTACATGGGAA420191.921360439701135No Hit
GCTTCCCATGTACTCTGCGTTGATA304951.3944141128700376No Hit
GTACTCTGCGTTGATACCACTGCTT228971.0469880289354074No Hit
GTGGTATCAACGCAGAGTACATGGG228341.0441072914666154No Hit
ATACCACTGCTTCCCATGTACTCTG221591.0132422471581297No Hit
CAGTGGTATCAACGCAGAGTACATG217480.9944488646236294No Hit
GGGAAGCAGTGGTATCAACGCAGAG212030.969528199219No Hit
ACATGGGAAGCAGTGGTATCAACGC210880.9642697101886654No Hit
CATGGGAAGCAGTGGTATCAACGCA207980.9510091726339086No Hit
GATACCACTGCTTCCCATGTACTCT201270.9203270322916953No Hit
TACCACTGCTTCCCATGTACTCTGC188770.8631695428315362No Hit
GCAGTGGTATCAACGCAGAGTACAT188610.8624379269664463No Hit
CTGCTTCCCATGTACTCTGCGTTGA188330.8611575992025386No Hit
GTATCAACGCAGAGTACTTTTTTTT187490.8573166159108161No Hit
GCAGAGTACATGGGAAGCAGTGGTA173080.7914254620611448No Hit
CCATGTACTCTGCGTTGATACCACT169200.7736837773327115No Hit
CTTCCCATGTACTCTGCGTTGATAC163490.7475742361473109No Hit
CCACTGCTTCCCATGTACTCTGCGT160250.7327590148792376No Hit
GTTGATACCACTGCTTCCCATGTAC158590.7251685002789285No Hit
GGTATCAACGCAGAGTACTTTTTTT155670.7118165107410355No Hit
TATCAACGCAGAGTACTTTTTTTTT152560.6975957273633479No Hit
GTACTTTTTTTTTTTTTTTTTTTTT150690.6890449669401081No Hit
AAGCAGTGGTATCAACGCAGAGTAC137960.6308357796738823No Hit
ATGGGAAGCAGTGGTATCAACGCAG132790.6071954420331604No Hit
TTCCCATGTACTCTGCGTTGATACC110300.5043576869964426No Hit
GTACATGGTAAGCAGTGGTATCAAC110000.5029859072493986No Hit
ATCAACGCAGAGTACATGGGAAGCA107300.49063988952600435No Hit
TGATACCACTGCTTCCCATGTACTC104880.47957419956651764No Hit
ACGCAGAGTACTTTTTTTTTTTTTT99720.45597958791736404No Hit
ACCATGTACTCTGCGTTGATACCAC98050.44834334732548675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA81570.3729869132212132No Hit
GAGTACTTTTTTTTTTTTTTTTTTT74670.3414359790392055No Hit
CCCCATGTACTCTGCGTTGATACCA67990.3108910166716965No Hit
GGAAGCAGTGGTATCAACGCAGAGT67120.30691285540526947No Hit
TCCATGTACTCTGCGTTGATACCAC60310.27577345514737484No Hit
GGTAAGCAGTGGTATCAACGCAGAG59980.27426449742562664No Hit
AAAAAGTACTCTGCGTTGATACCAC54450.24897802408845238No Hit
GTACATGGGTAAGCAGTGGTATCAA52540.2402443596989401No Hit
CTCTGCGTTGATACCACTGCTTCCC49300.2254291384308669No Hit
CTGCGTTGATACCACTGCTTCCCAT48670.22254840096207484No Hit
TCAACGCAGAGTACATGGGAAGCAG48560.22204541505482545No Hit
TGGGAAGCAGTGGTATCAACGCAGA46000.2103395612133849No Hit
TCCCATGTACTCTGCGTTGATACCA44600.20393792239384712No Hit
GAGTACATGGTAAGCAGTGGTATCA42390.193832478257291No Hit
AAAGTACTCTGCGTTGATACCACTG39580.1809834746266473No Hit
GTACATGGAAAGCAGTGGTATCAAC38330.1752677256806314No Hit
AGTGGTATCAACGCAGAGTACATGG38110.1742617538661326No Hit
GCTTACCATGTACTCTGCGTTGATA37670.17224981023713498No Hit
AACGCAGAGTACATGGGAAGCAGTG35230.16109266829451196No Hit
ACTCTGCGTTGATACCACTGCTTAC34800.1591264506570825No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA34590.15816620483415184No Hit
GCAGAGTACTTTTTTTTTTTTTTTT32350.14792358272289133No Hit
GTGGTATCAACGCAGAGTACTTTTT31340.14330525757451051No Hit
GCGTTGATACCACTGCTTACCATGT31130.14234501175157982No Hit
ACTGCTTCCCATGTACTCTGCGTTG30000.13717797470438148No Hit
GAGTACATGGAAAGCAGTGGTATCA29980.13708652272124522No Hit
CAACGCAGAGTACATGGGAAGCAGT29750.1360348249151783No Hit
ACGCAGAGTACATGGTAAGCAGTGG29450.1346630451681345No Hit
ATGTACTCTGCGTTGATACCACTGC27590.12615801073646282No Hit
TTGATACCACTGCTTCCCATGTACT27580.1261122847448947No Hit
CTGCTTACCATGTACTCTGCGTTGA27130.12405461512432897No Hit
CGCAGAGTACATGGGAAGCAGTGGT26370.12057943976515131No Hit
CGTTGATACCACTGCTTCCCATGTA25810.1180187842373362No Hit
TATCAACGCAGAGTACATGGTAAGC24680.11285174719013781No Hit
ATGGTAAGCAGTGGTATCAACGCAG24240.11083980356114023No Hit
GCTTTCCATGTACTCTGCGTTGATA24230.11079407756957209No Hit
ATCAACGCAGAGTACTTTTTTTTTT23860.1091022158815514No Hit
GTACATGGAAGCAGTGGTATCAACG23520.10754753216823507No Hit
GTATCAACGCAGAGTACATGGTAAG21900.10013992153419847No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTA401.2863076E-518.99878910
CCGTACA350.002171836316.28430417
GTACAAA3100.015.9578181
GGACGCA1750.015.7418548
TGTGCGC508.72127E-515.199038
GGGACGC1950.015.1016017
GCTTGGT456.7683327E-414.77649715
GATACGA653.3766773E-614.61411918
CGCAAAA2500.014.4387482
TGGGACG2050.014.3646096
GTGCGCT551.9617872E-413.8173029
ATACGAC707.272285E-613.57025319
ACGCAAA3600.013.4771961
CTGAGTC500.001500609113.2991519
TAAGGTG500.001500867713.2988475
AATACTG500.001500867713.2988475
TTGTGCG655.457734E-513.15300757
GAACAAA9550.013.0496781
TGATACG751.4805528E-512.66556917
GAACTCA604.096204E-412.6655686
Produced by FastQC (version 0.11.5)