##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294980_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2186940 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.859571821814956 33.0 33.0 33.0 27.0 33.0 2 31.749928667453155 33.0 33.0 33.0 27.0 33.0 3 31.980818861057003 33.0 33.0 33.0 27.0 33.0 4 35.63409375657311 37.0 37.0 37.0 33.0 37.0 5 35.98759408122765 37.0 37.0 37.0 33.0 37.0 6 35.455699744848964 37.0 37.0 37.0 33.0 37.0 7 35.40898972994229 37.0 37.0 37.0 33.0 37.0 8 35.39864239531034 37.0 37.0 37.0 33.0 37.0 9 35.28136803021574 37.0 37.0 37.0 33.0 37.0 10 35.37425032236824 37.0 37.0 37.0 33.0 37.0 11 35.32766239585906 37.0 37.0 37.0 33.0 37.0 12 35.385318298627304 37.0 37.0 37.0 33.0 37.0 13 35.18536951173786 37.0 37.0 37.0 33.0 37.0 14 36.899679460799106 40.0 37.0 40.0 33.0 40.0 15 36.83068899924095 40.0 37.0 40.0 33.0 40.0 16 36.72300657539759 40.0 37.0 40.0 33.0 40.0 17 36.83499638764667 40.0 37.0 40.0 33.0 40.0 18 36.823169817187484 40.0 37.0 40.0 33.0 40.0 19 36.56146945046503 40.0 37.0 40.0 33.0 40.0 20 36.700574318454095 40.0 37.0 40.0 33.0 40.0 21 36.7014463131133 40.0 37.0 40.0 33.0 40.0 22 36.813883325559914 40.0 37.0 40.0 33.0 40.0 23 36.77632079526644 40.0 37.0 40.0 33.0 40.0 24 36.80831298526709 40.0 37.0 40.0 33.0 40.0 25 35.82517444465783 37.0 37.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11501618770683564 1101 2 0.2047442513737039 1101 3 0.13494909442904301 1101 4 0.21560448891131756 1101 5 0.22646204604330933 1101 6 0.20047436685333508 1101 7 0.31593989812387235 1101 8 0.3736967970342988 1101 9 0.29244132197106865 1101 10 0.26540380994836 1101 11 0.33840530689787585 1101 12 0.3650189726291444 1101 13 0.38907916978754997 1101 14 0.5777084675685202 1101 15 0.48492133164554474 1101 16 0.5587404802784235 1101 17 0.529056169246509 1101 18 0.6357669272686337 1101 19 0.586896745002214 1101 20 0.5368824433868653 1101 21 0.5523629046235286 1101 22 0.6202204220123093 1101 23 0.592281135485095 1101 24 0.6721759978697861 1101 25 0.7180982231050237 1102 1 0.20546285428792643 1102 2 0.30308962960047126 1102 3 0.20635983741854247 1102 4 0.16273332576969324 1102 5 0.20699282006855668 1102 6 0.13907892779592856 1102 7 0.2559065518114352 1102 8 0.19401037788341569 1102 9 0.19451384235610192 1102 10 0.2624243212109363 1102 11 0.334060872865237 1102 12 0.32138779026132624 1102 13 0.3403852812338357 1102 14 0.6932783999773378 1102 15 0.5893708821549453 1102 16 0.6816779794761416 1102 17 0.7159755148144313 1102 18 0.6590323271177354 1102 19 0.5828150308477191 1102 20 0.5898237792646768 1102 21 0.5670104649086127 1102 22 0.5044100196173105 1102 23 0.8018808228455896 1102 24 0.7110305848419074 1102 25 0.7496853678020585 1103 1 0.07163288918301802 1103 2 0.10812245445598023 1103 3 0.07352569310049617 1103 4 0.07705635378692932 1103 5 0.20250861800569453 1103 6 0.10961825801619796 1103 7 0.03123210209831484 1103 8 0.10655894200987603 1103 9 0.17611052515972858 1103 10 0.18256648891874505 1103 11 0.22673767900322872 1103 12 0.16095073850760144 1103 13 0.15454859433862111 1103 14 0.3487944498781914 1103 15 0.4042632917667177 1103 16 0.38495036767476165 1103 17 0.4109586727321286 1103 18 0.3878261015831299 1103 19 0.40345907643735757 1103 20 0.3870244811528636 1103 21 0.4239446192676084 1103 22 0.5331379262538505 1103 23 0.5372359017760431 1103 24 0.5379750396281437 1103 25 0.5607072198256446 1104 1 0.1662456395260783 1104 2 0.18825641406256466 1104 3 0.09279523240276788 1104 4 0.15832263779822142 1104 5 0.17741952821769047 1104 6 0.24382042458312014 1104 7 0.1973694439431597 1104 8 0.1855605772117528 1104 9 0.3285402054339386 1104 10 0.3151493220193373 1104 11 0.37321254948677307 1104 12 0.3703967496426941 1104 13 0.3851033690676502 1104 14 0.6220292026030236 1104 15 0.4815473604875109 1104 16 0.6326577602380681 1104 17 0.6001138990307666 1104 18 0.6280405820362702 1104 19 0.5235243787230104 1104 20 0.5368317177523636 1104 21 0.40692763855754777 1104 22 0.4599520633448222 1104 23 0.5132938713449633 1104 24 0.5949841919225634 1104 25 0.6454052364255176 1105 1 -0.011214785620911272 1105 2 0.00333104092823433 1105 3 0.020368728558356253 1105 4 -0.005554567086448969 1105 5 0.06477932016703392 1105 6 0.13522928935019252 1105 7 -0.0034344909279369062 1105 8 0.08443552067624438 1105 9 0.007443416650581014 1105 10 -0.012118882413048482 1105 11 -0.021876776610014304 1105 12 -0.043405828376300803 1105 13 -0.05141517457775535 1105 14 0.023651771576339797 1105 15 -0.023569102651336493 1105 16 0.01979480230048125 1105 17 0.07155442368024723 1105 18 0.12669277561383296 1105 19 0.11473084638470255 1105 20 0.22677631295815814 1105 21 0.11907146488047715 1105 22 0.07528675353025704 1105 23 0.1700747397303104 1105 24 0.1589529843241948 1105 25 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2216 23 -0.726272102777898 2216 24 -0.7580930259003011 2216 25 -0.9265039624862013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 8.0 8 48.0 9 103.0 10 158.0 11 309.0 12 735.0 13 1454.0 14 2961.0 15 5440.0 16 7722.0 17 8858.0 18 9294.0 19 9339.0 20 9360.0 21 10117.0 22 10867.0 23 11396.0 24 11999.0 25 13119.0 26 13524.0 27 14875.0 28 16355.0 29 19572.0 30 24851.0 31 33586.0 32 46302.0 33 67456.0 34 100948.0 35 168650.0 36 335542.0 37 1231992.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.68119538566847 18.31850242552305 10.449231135520234 25.55107105328825 2 10.055379786629377 24.88171607376472 32.00916000235951 33.05374413724639 3 23.449431625924806 31.17877033663475 25.966007297868256 19.405790739572186 4 13.570925585521321 23.515185601799775 26.51353032090501 36.40035849177389 5 9.760197318332652 40.691418947992226 36.605815568266465 12.942568165408657 6 34.71791635801622 26.429668852369065 18.87692392109523 19.975490868519483 7 19.845217518541887 34.68755429961499 24.53364061199667 20.933587569846452 8 29.95673355239392 32.420433542425535 22.763583753179137 14.859249152001405 9 28.22770629281096 15.97172304681427 19.89085205812688 35.90971860224789 10 18.594200115229498 31.741657292838394 31.368533201642478 18.295609390289627 11 24.668806643072056 27.234217674010257 16.12046969738539 31.976505985532295 12 25.34436244249957 34.35508061492313 27.244597474096228 13.055959468481074 13 32.849120474165225 18.905198505475973 21.66904368112736 26.57663733923144 14 22.08583683137169 29.24826469861999 24.833465938708883 23.832432531299443 15 40.811361994384846 16.89438210467594 21.22829158550303 21.06596431543618 16 15.499556457881788 25.425891885465536 38.760368368588075 20.3141832880646 17 27.63441155221451 29.787557043174477 28.55336680475916 14.024664599851846 18 29.25224285988642 20.14792358272289 31.590624342688873 19.009209214701823 19 22.99061702653022 32.88668184769587 32.01761365195204 12.10508747382187 20 24.391661408177637 23.634621891775723 29.715447154471548 22.258269545575097 21 16.316314119271677 30.609618919586275 33.40407144228922 19.669995518852826 22 21.165692703046265 28.66182885675876 19.590660923482126 30.58181751671285 23 14.038885383229536 28.160626263180514 30.479391295600244 27.3210970579897 24 20.657928971436124 39.164616221055795 22.632575541068434 17.54487926643965 25 21.240495181115698 22.181134446346157 20.571699794733867 36.00667057780428 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10122.0 1 10122.0 2 9743.5 3 9365.0 4 9365.0 5 9365.0 6 16983.5 7 24602.0 8 24602.0 9 24602.0 10 20375.0 11 16148.0 12 16148.0 13 16148.0 14 13072.5 15 9997.0 16 9997.0 17 9997.0 18 13331.5 19 16666.0 20 16666.0 21 16666.0 22 24716.5 23 32767.0 24 32767.0 25 32767.0 26 42383.0 27 51999.0 28 51999.0 29 51999.0 30 56035.5 31 60072.0 32 60072.0 33 60072.0 34 62847.0 35 65622.0 36 65622.0 37 65622.0 38 66072.0 39 66522.0 40 66522.0 41 66522.0 42 103319.0 43 140116.0 44 140116.0 45 140116.0 46 496269.5 47 852423.0 48 852423.0 49 852423.0 50 720245.5 51 588068.0 52 588068.0 53 588068.0 54 376334.5 55 164601.0 56 164601.0 57 164601.0 58 100796.5 59 36992.0 60 36992.0 61 36992.0 62 29421.5 63 21851.0 64 21851.0 65 21851.0 66 16590.0 67 11329.0 68 11329.0 69 11329.0 70 8092.5 71 4856.0 72 4856.0 73 4856.0 74 3418.0 75 1980.0 76 1980.0 77 1980.0 78 1319.5 79 659.0 80 659.0 81 659.0 82 400.5 83 142.0 84 142.0 85 142.0 86 86.0 87 30.0 88 30.0 89 30.0 90 20.0 91 10.0 92 10.0 93 10.0 94 5.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13146222575836558 2 0.0018747656542932134 3 0.0 4 0.0 5 7.773418566581616E-4 6 0.0 7 0.0 8 0.0011888757807713062 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0010516978060669247 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 7.773418566581616E-4 25 0.0010516978060669247 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2186940.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.682298724601324 #Duplication Level Percentage of deduplicated Percentage of total 1 70.89954287463779 9.700687250484734 2 13.894628394894074 3.8022091273253706 3 5.87174160724999 2.4101676811219517 4 3.0208548270525055 1.6532895258954499 5 1.7578589507012656 1.2025775639604472 6 1.089487302416604 0.8944014436994423 7 0.710304595363056 0.680301976145008 8 0.5207167725187065 0.5699681946008973 9 0.36490461380097383 0.44934605388091825 >10 1.5863716690622314 3.8362101226460346 >50 0.11928185681076876 1.1409638183500552 >100 0.11222573180842735 3.0676559283598253 >500 0.013776212587261473 1.3598422633259897 >1k 0.022512347398695575 7.083877576291009 >5k 0.003360051850551579 3.2935224228227424 >10k+ 0.012432191847040842 58.85497905109014 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 192659 8.80952380952381 No Hit GTACATGGGAAGCAGTGGTATCAAC 166189 7.599156812715484 No Hit GAGTACATGGGAAGCAGTGGTATCA 81764 3.7387399745763488 No Hit CATGTACTCTGCGTTGATACCACTG 68995 3.154864788242933 No Hit GCGTTGATACCACTGCTTCCCATGT 58010 2.6525647708670563 No Hit ACGCAGAGTACATGGGAAGCAGTGG 55157 2.5221085169231894 No Hit TATCAACGCAGAGTACATGGGAAGC 47640 2.1783862383055776 No Hit ACTCTGCGTTGATACCACTGCTTCC 46254 2.1150100139921535 No Hit GTATCAACGCAGAGTACATGGGAAG 44169 2.0196713215726083 No Hit GGTATCAACGCAGAGTACATGGGAA 42019 1.921360439701135 No Hit GCTTCCCATGTACTCTGCGTTGATA 30495 1.3944141128700376 No Hit GTACTCTGCGTTGATACCACTGCTT 22897 1.0469880289354074 No Hit GTGGTATCAACGCAGAGTACATGGG 22834 1.0441072914666154 No Hit ATACCACTGCTTCCCATGTACTCTG 22159 1.0132422471581297 No Hit CAGTGGTATCAACGCAGAGTACATG 21748 0.9944488646236294 No Hit GGGAAGCAGTGGTATCAACGCAGAG 21203 0.969528199219 No Hit ACATGGGAAGCAGTGGTATCAACGC 21088 0.9642697101886654 No Hit CATGGGAAGCAGTGGTATCAACGCA 20798 0.9510091726339086 No Hit GATACCACTGCTTCCCATGTACTCT 20127 0.9203270322916953 No Hit TACCACTGCTTCCCATGTACTCTGC 18877 0.8631695428315362 No Hit GCAGTGGTATCAACGCAGAGTACAT 18861 0.8624379269664463 No Hit CTGCTTCCCATGTACTCTGCGTTGA 18833 0.8611575992025386 No Hit GTATCAACGCAGAGTACTTTTTTTT 18749 0.8573166159108161 No Hit GCAGAGTACATGGGAAGCAGTGGTA 17308 0.7914254620611448 No Hit CCATGTACTCTGCGTTGATACCACT 16920 0.7736837773327115 No Hit CTTCCCATGTACTCTGCGTTGATAC 16349 0.7475742361473109 No Hit CCACTGCTTCCCATGTACTCTGCGT 16025 0.7327590148792376 No Hit GTTGATACCACTGCTTCCCATGTAC 15859 0.7251685002789285 No Hit GGTATCAACGCAGAGTACTTTTTTT 15567 0.7118165107410355 No Hit TATCAACGCAGAGTACTTTTTTTTT 15256 0.6975957273633479 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 15069 0.6890449669401081 No Hit AAGCAGTGGTATCAACGCAGAGTAC 13796 0.6308357796738823 No Hit ATGGGAAGCAGTGGTATCAACGCAG 13279 0.6071954420331604 No Hit TTCCCATGTACTCTGCGTTGATACC 11030 0.5043576869964426 No Hit GTACATGGTAAGCAGTGGTATCAAC 11000 0.5029859072493986 No Hit ATCAACGCAGAGTACATGGGAAGCA 10730 0.49063988952600435 No Hit TGATACCACTGCTTCCCATGTACTC 10488 0.47957419956651764 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 9972 0.45597958791736404 No Hit ACCATGTACTCTGCGTTGATACCAC 9805 0.44834334732548675 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 8157 0.3729869132212132 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 7467 0.3414359790392055 No Hit CCCCATGTACTCTGCGTTGATACCA 6799 0.3108910166716965 No Hit GGAAGCAGTGGTATCAACGCAGAGT 6712 0.30691285540526947 No Hit TCCATGTACTCTGCGTTGATACCAC 6031 0.27577345514737484 No Hit GGTAAGCAGTGGTATCAACGCAGAG 5998 0.27426449742562664 No Hit AAAAAGTACTCTGCGTTGATACCAC 5445 0.24897802408845238 No Hit GTACATGGGTAAGCAGTGGTATCAA 5254 0.2402443596989401 No Hit CTCTGCGTTGATACCACTGCTTCCC 4930 0.2254291384308669 No Hit CTGCGTTGATACCACTGCTTCCCAT 4867 0.22254840096207484 No Hit TCAACGCAGAGTACATGGGAAGCAG 4856 0.22204541505482545 No Hit TGGGAAGCAGTGGTATCAACGCAGA 4600 0.2103395612133849 No Hit TCCCATGTACTCTGCGTTGATACCA 4460 0.20393792239384712 No Hit GAGTACATGGTAAGCAGTGGTATCA 4239 0.193832478257291 No Hit AAAGTACTCTGCGTTGATACCACTG 3958 0.1809834746266473 No Hit GTACATGGAAAGCAGTGGTATCAAC 3833 0.1752677256806314 No Hit AGTGGTATCAACGCAGAGTACATGG 3811 0.1742617538661326 No Hit GCTTACCATGTACTCTGCGTTGATA 3767 0.17224981023713498 No Hit AACGCAGAGTACATGGGAAGCAGTG 3523 0.16109266829451196 No Hit ACTCTGCGTTGATACCACTGCTTAC 3480 0.1591264506570825 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 3459 0.15816620483415184 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 3235 0.14792358272289133 No Hit GTGGTATCAACGCAGAGTACTTTTT 3134 0.14330525757451051 No Hit GCGTTGATACCACTGCTTACCATGT 3113 0.14234501175157982 No Hit ACTGCTTCCCATGTACTCTGCGTTG 3000 0.13717797470438148 No Hit GAGTACATGGAAAGCAGTGGTATCA 2998 0.13708652272124522 No Hit CAACGCAGAGTACATGGGAAGCAGT 2975 0.1360348249151783 No Hit ACGCAGAGTACATGGTAAGCAGTGG 2945 0.1346630451681345 No Hit ATGTACTCTGCGTTGATACCACTGC 2759 0.12615801073646282 No Hit TTGATACCACTGCTTCCCATGTACT 2758 0.1261122847448947 No Hit CTGCTTACCATGTACTCTGCGTTGA 2713 0.12405461512432897 No Hit CGCAGAGTACATGGGAAGCAGTGGT 2637 0.12057943976515131 No Hit CGTTGATACCACTGCTTCCCATGTA 2581 0.1180187842373362 No Hit TATCAACGCAGAGTACATGGTAAGC 2468 0.11285174719013781 No Hit ATGGTAAGCAGTGGTATCAACGCAG 2424 0.11083980356114023 No Hit GCTTTCCATGTACTCTGCGTTGATA 2423 0.11079407756957209 No Hit ATCAACGCAGAGTACTTTTTTTTTT 2386 0.1091022158815514 No Hit GTACATGGAAGCAGTGGTATCAACG 2352 0.10754753216823507 No Hit GTATCAACGCAGAGTACATGGTAAG 2190 0.10013992153419847 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 4.5725991568127154E-5 0.0 0.0 0.0 0.0 9 4.5725991568127154E-5 0.0 0.0 0.0 0.0 10 4.5725991568127154E-5 0.0 0.0 4.5725991568127154E-5 0.0 11 4.5725991568127154E-5 0.0 0.0 4.5725991568127154E-5 0.0 12 4.5725991568127154E-5 0.0 0.0 9.145198313625431E-5 0.0 13 4.5725991568127154E-5 0.0 0.0 1.3717797470438146E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGCTA 40 1.2863076E-5 18.998789 10 CCGTACA 35 0.0021718363 16.284304 17 GTACAAA 310 0.0 15.957818 1 GGACGCA 175 0.0 15.741854 8 TGTGCGC 50 8.72127E-5 15.19903 8 GGGACGC 195 0.0 15.101601 7 GCTTGGT 45 6.7683327E-4 14.776497 15 GATACGA 65 3.3766773E-6 14.614119 18 CGCAAAA 250 0.0 14.438748 2 TGGGACG 205 0.0 14.364609 6 GTGCGCT 55 1.9617872E-4 13.817302 9 ATACGAC 70 7.272285E-6 13.570253 19 ACGCAAA 360 0.0 13.477196 1 CTGAGTC 50 0.0015006091 13.299151 9 TAAGGTG 50 0.0015008677 13.298847 5 AATACTG 50 0.0015008677 13.298847 5 TTGTGCG 65 5.457734E-5 13.1530075 7 GAACAAA 955 0.0 13.049678 1 TGATACG 75 1.4805528E-5 12.665569 17 GAACTCA 60 4.096204E-4 12.665568 6 >>END_MODULE