FastQCFastQC Report
Wed 25 May 2016
SRR1294979_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294979_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6421601
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC468950.7302696009920268No Hit
CCCATGTACTCTGCGTTGATACCAC451350.7028621055714922No Hit
GAGTACATGGGAAGCAGTGGTATCA207690.32342401840288737No Hit
CATGTACTCTGCGTTGATACCACTG158540.24688547295292873No Hit
GCGTTGATACCACTGCTTCCCATGT144850.2255668017991152No Hit
GTATCAACGCAGAGTACATGGGAAG136030.21183190920768827No Hit
ACGCAGAGTACATGGGAAGCAGTGG122490.19074682466257248No Hit
TATCAACGCAGAGTACATGGGAAGC108910.1695994503551373No Hit
GCTTCCCATGTACTCTGCGTTGATA108500.16896098029136347No Hit
GGTATCAACGCAGAGTACATGGGAA106600.16600221658119213No Hit
ACTCTGCGTTGATACCACTGCTTCC101740.15843401045938543No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN76570.11923817751990508No Hit
GTGGTATCAACGCAGAGTACATGGG75160.11704246339814635No Hit
GTATCAACGCAGAGTACTTTTTTTT74390.11584338547349796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCT1001.9345898E-611.398978
TAGACAG10050.09.9305215
TATTCCG2700.09.5049285
TATACCG1106.804944E-59.5049275
TACACCG1651.05914296E-79.2168995
GTCCTAC3750.09.1310751
TGTGCGC2201.6916601E-109.0670810
GGACCGG850.0074493758.9398116
ATCGTTC1107.156432E-48.6355838
GTGCGCC2106.4283086E-98.59265111
CGCGTGC1000.00291468928.54749312
CTACACT10400.08.500134
CTAGGAC6300.08.4485613
CCTACAC6100.08.4139363
ATAAGAC6450.08.3994413
TTAGACA8150.08.3975154
GTATTAG9650.08.3780381
GGACCGT1252.7503102E-48.3587236
GACCGTG2053.8762664E-88.3405157
GAGGCGT1150.00111311318.2596086
Produced by FastQC (version 0.11.5)