##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294979_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6421601 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.091738648975543 33.0 33.0 33.0 27.0 33.0 2 31.015484456290572 33.0 33.0 33.0 27.0 33.0 3 31.116617802943534 33.0 33.0 33.0 27.0 33.0 4 34.69049618623144 37.0 37.0 37.0 27.0 37.0 5 34.91377633085581 37.0 37.0 37.0 33.0 37.0 6 34.78052435833369 37.0 37.0 37.0 27.0 37.0 7 34.708634965018845 37.0 37.0 37.0 27.0 37.0 8 34.64086884252074 37.0 37.0 37.0 27.0 37.0 9 34.64236504261165 37.0 37.0 37.0 27.0 37.0 10 34.54540713445136 37.0 37.0 37.0 27.0 37.0 11 34.71276181749691 37.0 37.0 37.0 27.0 37.0 12 34.62654266436049 37.0 37.0 37.0 27.0 37.0 13 34.54578756917473 37.0 37.0 37.0 27.0 37.0 14 35.88762194972874 40.0 37.0 40.0 27.0 40.0 15 36.03662077416519 40.0 37.0 40.0 27.0 40.0 16 36.11222092434581 40.0 37.0 40.0 27.0 40.0 17 36.03740796103651 40.0 37.0 40.0 27.0 40.0 18 35.97454637869902 37.0 37.0 40.0 27.0 40.0 19 36.060068820843895 40.0 37.0 40.0 27.0 40.0 20 36.077616469786896 40.0 37.0 40.0 27.0 40.0 21 36.06681916238645 40.0 37.0 40.0 27.0 40.0 22 35.964046660638054 40.0 37.0 40.0 27.0 40.0 23 35.863193617915535 37.0 37.0 40.0 27.0 40.0 24 35.928588057713334 37.0 37.0 40.0 27.0 40.0 25 34.74205824995978 37.0 33.0 40.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44047119525399836 1101 2 0.417243255322969 1101 3 0.42352911254816306 1101 4 0.4950291751659961 1101 5 0.39507182089029413 1101 6 0.3028566107080479 1101 7 0.3126117398040478 1101 8 0.4411681640466867 1101 9 0.37376871281123414 1101 10 0.5627021126186804 1101 11 0.4853196507327766 1101 12 0.537003219725726 1101 13 0.47579416037237365 1101 14 0.9264860666915524 1101 15 0.8331668479637244 1101 16 0.7470785606790571 1101 17 0.7154707856621343 1101 18 0.7169928473125111 1101 19 0.6573564993223684 1101 20 0.6590081435350257 1101 21 0.635260707864937 1101 22 0.7241747255127464 1101 23 0.7484344426149292 1101 24 0.8027914405423786 1101 25 0.8457181932116029 1102 1 0.3740948466383429 1102 2 0.36518320859370945 1102 3 0.39152548654227104 1102 4 0.44921774652348034 1102 5 0.3451864811674099 1102 6 0.24412147891793268 1102 7 0.2641441688324022 1102 8 0.37100614918158215 1102 9 0.3534959375640909 1102 10 0.5777036977915486 1102 11 0.4617480909641358 1102 12 0.48472640382868093 1102 13 0.4018471404469395 1102 14 0.8564112055160464 1102 15 0.6631368355815539 1102 16 0.6350359536529879 1102 17 0.6919649077469998 1102 18 0.7721124737922125 1102 19 0.619955821973349 1102 20 0.7061120736094537 1102 21 0.6716406661248513 1102 22 0.705041123064241 1102 23 0.8116045731840842 1102 24 0.7969274664170598 1102 25 0.8450580463177957 1103 1 0.3447607216356978 1103 2 0.320962063970363 1103 3 0.31524543749051404 1103 4 0.3531077476871829 1103 5 0.13740277615502805 1103 6 0.09616137182217699 1103 7 0.162765205196294 1103 8 0.35347131308710544 1103 9 0.3496395470824609 1103 10 0.49329458019427364 1103 11 0.31761325416438524 1103 12 0.4229466421758019 1103 13 0.4150938926632577 1103 14 0.7167023555699998 1103 15 0.6825564926071337 1103 16 0.6321816463724517 1103 17 0.5896062974175678 1103 18 0.5783546937458084 1103 19 0.5694625273595193 1103 20 0.5292955980718617 1103 21 0.540758113529094 1103 22 0.6335848791031466 1103 23 0.6662326649260422 1103 24 0.6664072479939094 1103 25 0.7608644558418121 1104 1 0.28257518031560735 1104 2 0.20197264612689736 1104 3 0.2512009763803533 1104 4 0.2771048120520234 1104 5 0.16595531062710478 1104 6 0.06991745646980263 1104 7 0.15873192942240877 1104 8 0.29773309850945395 1104 9 0.22000705533052667 1104 10 0.43138780218642125 1104 11 0.27331684971375836 1104 12 0.35793156354158384 1104 13 0.32430083977455837 1104 14 0.6005970761703381 1104 15 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1108 24 -0.4053897819750176 1108 25 -1.18294203065998 1109 1 0.08994905449840829 1109 2 -0.05422902840886579 1109 3 0.09587571744352275 1109 4 0.14594897908821025 1109 5 0.08972781723479528 1109 6 0.08297508898986905 1109 7 0.17873761835047475 1109 8 0.2398609822617459 1109 9 0.21492185293171673 1109 10 0.26105973003282656 1109 11 0.18234199082355929 1109 12 0.24846478148385387 1109 13 0.2240997906704294 1109 14 0.492380428103651 1109 15 0.3756061577658727 1109 16 0.22628157350150957 1109 17 0.27374952508830575 1109 18 0.34515887921310195 1109 19 0.3105637630478384 1109 20 0.2550497138021015 1109 21 0.3329135547964981 1109 22 0.3333701291097668 1109 23 0.3713409084051307 1109 24 0.4229752244206182 1109 25 0.4454635509562408 1110 1 0.11193446600465506 1110 2 0.09210051110277107 1110 3 0.1638606709429382 1110 4 -0.8578978766173577 1110 5 -0.22780164926478363 1110 6 0.049625026839471786 1110 7 0.13893172431726697 1110 8 0.2743081963672296 1110 9 0.1949910453119088 1110 10 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-0.02633715800027403 2214 14 0.04136383009046796 2214 15 -0.03179264181297725 2214 16 -0.12416833479068146 2214 17 -0.1265277978572783 2214 18 0.04544480952078089 2214 19 -0.8112079566471522 2214 20 -0.2619633563081223 2214 21 -0.18263576897771827 2214 22 -0.4342969268972965 2214 23 -1.715867871420862 2214 24 -1.1557624825381723 2214 25 -1.5376212372998594 2215 1 -0.355754524699055 2215 2 -0.16873001343063976 2215 3 -1.138019472168903 2215 4 -0.8090307345988421 2215 5 -0.21649560747067653 2215 6 -0.15127376581777696 2215 7 -0.14240474402341619 2215 8 -0.0013609325472287992 2215 9 -0.2361099717583528 2215 10 -1.3479218206957029 2215 11 -0.5205076691380555 2215 12 -0.5100948951535003 2215 13 -0.48112824032659773 2215 14 -0.7038569795079255 2215 15 -1.02406155183008 2215 16 -0.6369401878359966 2215 17 -0.4988750510862445 2215 18 -0.3082197685111723 2215 19 -0.8798104678760623 2215 20 -1.2761104605116103 2215 21 -0.5856079619660264 2215 22 -0.2785661349451303 2215 23 -0.5885545438607522 2215 24 -1.7114926547157623 2215 25 -0.6332672922569955 2216 1 -0.501569784201962 2216 2 -0.4198844606016827 2216 3 -1.2939736941981828 2216 4 -0.6948570727091763 2216 5 -0.5753662539048179 2216 6 -0.42271337120313035 2216 7 -0.27742851076617114 2216 8 -0.4611573681528114 2216 9 -0.23608475389992378 2216 10 -0.2864101949683473 2216 11 -0.3525124022047166 2216 12 -0.3287001921591113 2216 13 -1.0307901583415742 2216 14 -0.6808157390864764 2216 15 -1.066209235482674 2216 16 -0.8355322405555867 2216 17 -1.781087054368406 2216 18 -0.8027772585033404 2216 19 -0.5873428321109202 2216 20 -0.5280759184503694 2216 21 -0.3036690767157779 2216 22 -0.4218217775476987 2216 23 -0.25574722448195786 2216 24 -0.4503152061763558 2216 25 -0.31770037144332974 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8527.0 3 893.0 4 1102.0 5 972.0 6 636.0 7 490.0 8 875.0 9 1907.0 10 3776.0 11 6706.0 12 11231.0 13 16384.0 14 21346.0 15 26636.0 16 30426.0 17 32647.0 18 33635.0 19 35058.0 20 36882.0 21 40400.0 22 43208.0 23 46278.0 24 49688.0 25 52632.0 26 55565.0 27 62520.0 28 70157.0 29 82321.0 30 99562.0 31 125986.0 32 166460.0 33 234025.0 34 337419.0 35 531088.0 36 957003.0 37 3197160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.53816060815152 20.576821026423623 13.586571502528106 23.29844686289675 2 14.857412083330408 20.92169852751328 40.14970580687233 24.071183582283982 3 18.507424818321443 26.050784955285067 29.526617607383955 25.915172619009542 4 11.950027218494592 17.64687062225474 38.06581382050277 32.337288338747896 5 12.821105643741179 38.29006444280701 35.463354397971806 13.425475515480013 6 31.314508191451978 37.54797253059663 17.716460733520197 13.421058544431192 7 26.24008195778756 32.608786215588864 23.258642756694993 17.892489069928587 8 24.723546458412308 36.56641868808306 21.190471991643772 17.51956286186086 9 24.41414793887228 17.161875531885176 21.84244946612891 36.58152706311363 10 14.560119169509386 30.44051139002792 33.922854474350586 21.076514966112107 11 31.76806239987092 25.887779286686975 22.78927248262999 19.554885830812115 12 21.247071935318306 27.923316890448756 31.434216836034462 19.395394338198475 13 26.81175389119785 23.565715320230794 26.332621137089713 23.289909651481643 14 20.439409532711885 23.81316277955633 28.024428286188908 27.72299940154288 15 23.95054300243153 29.946686693549836 23.883821400189337 22.2189489038293 16 21.287990109452334 31.163058436215536 26.504464431306772 21.044487023025358 17 20.57513424240968 30.244535586487814 28.49985238484301 20.680477786259495 18 20.782939163093445 29.629618648524403 29.313310802186038 20.27413138619612 19 21.020195623943383 29.138860792164305 29.63378793330897 20.207155650583342 20 22.381347457162967 28.489811191095033 28.6270944156873 20.501746936054698 21 22.06509483452447 29.112999087808493 28.080362729534126 20.74154334813291 22 21.141831186111347 28.776925317932566 28.38087400306436 21.70036949289172 23 20.629756709875107 29.371398039672204 28.474407316992483 21.52443793346021 24 21.141329777388222 29.308906812200714 28.31791185613655 21.231851554274517 25 21.70594547556563 28.260251422664247 27.284565310214465 22.749237791555654 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 12692.0 1 12692.0 2 10038.0 3 7384.0 4 7384.0 5 7384.0 6 9746.0 7 12108.0 8 12108.0 9 12108.0 10 16711.0 11 21314.0 12 21314.0 13 21314.0 14 34757.5 15 48201.0 16 48201.0 17 48201.0 18 80965.0 19 113729.0 20 113729.0 21 113729.0 22 168125.0 23 222521.0 24 222521.0 25 222521.0 26 295420.0 27 368319.0 28 368319.0 29 368319.0 30 446494.5 31 524670.0 32 524670.0 33 524670.0 34 587286.0 35 649902.0 36 649902.0 37 649902.0 38 691885.5 39 733869.0 40 733869.0 41 733869.0 42 738650.0 43 743431.0 44 743431.0 45 743431.0 46 809189.5 47 874948.0 48 874948.0 49 874948.0 50 802963.5 51 730979.0 52 730979.0 53 730979.0 54 631492.0 55 532005.0 56 532005.0 57 532005.0 58 454268.0 59 376531.0 60 376531.0 61 376531.0 62 308616.0 63 240701.0 64 240701.0 65 240701.0 66 182675.5 67 124650.0 68 124650.0 69 124650.0 70 89231.5 71 53813.0 72 53813.0 73 53813.0 74 37057.5 75 20302.0 76 20302.0 77 20302.0 78 13528.0 79 6754.0 80 6754.0 81 6754.0 82 4394.5 83 2035.0 84 2035.0 85 2035.0 86 1286.5 87 538.0 88 538.0 89 538.0 90 339.0 91 140.0 92 140.0 93 140.0 94 86.5 95 33.0 96 33.0 97 33.0 98 32.5 99 32.0 100 32.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31409612649555774 2 0.18213526502191588 3 0.2007599039554155 4 0.22101964914979927 5 0.2479288264717786 6 0.19815930637858067 7 0.19384574033796245 8 0.21683066263381984 9 0.2175158500193332 10 0.22724862538173893 11 0.20241058265687947 12 0.20801666126562518 13 0.22098850426863956 14 0.2095427604424504 15 0.20060417954961698 16 0.20334492909167046 17 0.20314248736413243 18 0.20691101798445588 19 0.20529148416415158 20 0.20169425039020644 21 0.20024601341628048 22 0.2038121023090659 23 0.20687987310329622 24 0.22273261761358265 25 0.2165347862628027 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 6421601.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.432283633292 #Duplication Level Percentage of deduplicated Percentage of total 1 85.97917987464346 63.1364752311265 2 10.363087431342079 15.219703511498297 3 2.134361315749069 4.701930765420357 4 0.6507479088518495 1.9114362006632266 5 0.2893523372544877 1.0623901449613753 6 0.14165242448894755 0.6241116607449525 7 0.08872169395641982 0.45605256165237645 8 0.05329371352952288 0.31307832702170685 9 0.03789663525162448 0.2504552821690231 >10 0.22893822864080648 3.075342162965388 >50 0.018404630045043516 0.9373172159726393 >100 0.011796283712158453 1.742150409492872 >500 0.0011767989319250512 0.6136371294289281 >1k 0.0010056001438186796 1.922350941865694 >5k 1.4977023403263366E-4 0.7095890440985287 >10k+ 2.353532249084243E-4 3.3239794109181036 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 46895 0.7302696009920268 No Hit CCCATGTACTCTGCGTTGATACCAC 45135 0.7028621055714922 No Hit GAGTACATGGGAAGCAGTGGTATCA 20769 0.32342401840288737 No Hit CATGTACTCTGCGTTGATACCACTG 15854 0.24688547295292873 No Hit GCGTTGATACCACTGCTTCCCATGT 14485 0.2255668017991152 No Hit GTATCAACGCAGAGTACATGGGAAG 13603 0.21183190920768827 No Hit ACGCAGAGTACATGGGAAGCAGTGG 12249 0.19074682466257248 No Hit TATCAACGCAGAGTACATGGGAAGC 10891 0.1695994503551373 No Hit GCTTCCCATGTACTCTGCGTTGATA 10850 0.16896098029136347 No Hit GGTATCAACGCAGAGTACATGGGAA 10660 0.16600221658119213 No Hit ACTCTGCGTTGATACCACTGCTTCC 10174 0.15843401045938543 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 7657 0.11923817751990508 No Hit GTGGTATCAACGCAGAGTACATGGG 7516 0.11704246339814635 No Hit GTATCAACGCAGAGTACTTTTTTTT 7439 0.11584338547349796 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.557244057984917E-5 0.0 4 0.0 0.0 0.0 3.114488115969834E-5 0.0 5 0.0 0.0 0.0 3.114488115969834E-5 0.0 6 0.0 0.0 0.0 3.114488115969834E-5 0.0 7 0.0 0.0 0.0 3.114488115969834E-5 0.0 8 0.0 0.0 0.0 7.786220289924585E-5 0.0 9 0.0 0.0 0.0 1.557244057984917E-4 0.0 10 0.0 0.0 0.0 1.557244057984917E-4 0.0 11 0.0 0.0 0.0 1.557244057984917E-4 0.0 12 0.0 1.557244057984917E-5 0.0 1.8686928695819003E-4 0.0 13 0.0 1.557244057984917E-5 0.0 2.0244172753803918E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCT 100 1.9345898E-6 11.39897 8 TAGACAG 1005 0.0 9.930521 5 TATTCCG 270 0.0 9.504928 5 TATACCG 110 6.804944E-5 9.504927 5 TACACCG 165 1.05914296E-7 9.216899 5 GTCCTAC 375 0.0 9.131075 1 TGTGCGC 220 1.6916601E-10 9.06708 10 GGACCGG 85 0.007449375 8.939811 6 ATCGTTC 110 7.156432E-4 8.635583 8 GTGCGCC 210 6.4283086E-9 8.592651 11 CGCGTGC 100 0.0029146892 8.547493 12 CTACACT 1040 0.0 8.50013 4 CTAGGAC 630 0.0 8.448561 3 CCTACAC 610 0.0 8.413936 3 ATAAGAC 645 0.0 8.399441 3 TTAGACA 815 0.0 8.397515 4 GTATTAG 965 0.0 8.378038 1 GGACCGT 125 2.7503102E-4 8.358723 6 GACCGTG 205 3.8762664E-8 8.340515 7 GAGGCGT 115 0.0011131131 8.259608 6 >>END_MODULE