FastQCFastQC Report
Wed 25 May 2016
SRR1294979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294979_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6421601
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC503610.7842436800417839No Hit
GTACATGGGAAGCAGTGGTATCAAC450570.7016474552062639No Hit
GAGTACATGGGAAGCAGTGGTATCA221400.3447738344378606No Hit
GTATCAACGCAGAGTACTTTTTTTT208230.3242649301941992No Hit
CATGTACTCTGCGTTGATACCACTG181200.28217262330686693No Hit
GGTATCAACGCAGAGTACTTTTTTT167340.26058922066319595No Hit
GCGTTGATACCACTGCTTCCCATGT161250.25110560435006785No Hit
ACGCAGAGTACATGGGAAGCAGTGG146560.22822968913826944No Hit
GTATCAACGCAGAGTACATGGGAAG136320.21228350998450385No Hit
TATCAACGCAGAGTACTTTTTTTTT132710.20666185893517833No Hit
GCTTCCCATGTACTCTGCGTTGATA119130.18551448462774314No Hit
ACTCTGCGTTGATACCACTGCTTCC117460.18291388705090833No Hit
TATCAACGCAGAGTACATGGGAAGC117280.18263358312047104No Hit
GGTATCAACGCAGAGTACATGGGAA110980.17282294555516609No Hit
ACGCAGAGTACTTTTTTTTTTTTTT81230.12649493483011479No Hit
GTGGTATCAACGCAGAGTACATGGG79630.12400334433733892No Hit
CAGTGGTATCAACGCAGAGTACATG66050.10285597002990376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCG1856.002665E-1110.2697825
TAGGACC4850.09.9892164
TACACCG1356.4575215E-79.8513835
GCACCGT1651.0253643E-89.7872616
CTAGGAC6700.09.4995493
TATACCG1401.0609001E-69.4995485
CTGCGAA800.00452134539.4993269
TCTAGAC6550.09.136973
CCGGGAC1252.7587626E-59.1195673
GCTCTAG4500.09.0881021
TAGACAG10200.09.0338845
CCAGGAC10250.08.8971383
TAGACTG9100.08.8732045
GTATACG1304.315509E-58.7792051
ACCGTGT3700.08.7291798
GTCGCCC5550.08.72911111
ACTGTCG1201.766664E-48.7077848
TAGGACT8000.08.6683394
CTAGACA8700.08.5168364
CCTACAC8200.08.4569153
Produced by FastQC (version 0.11.5)