FastQCFastQC Report
Wed 25 May 2016
SRR1294978_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294978_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6218842
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC553640.890262206372183No Hit
CCCATGTACTCTGCGTTGATACCAC517140.8315696073320402No Hit
GAGTACATGGGAAGCAGTGGTATCA250020.4020362633429182No Hit
CATGTACTCTGCGTTGATACCACTG187560.3015995582457313No Hit
GCGTTGATACCACTGCTTCCCATGT170030.27341103054234217No Hit
GTATCAACGCAGAGTACATGGGAAG158600.2550314029525111No Hit
ACGCAGAGTACATGGGAAGCAGTGG143950.231473962515851No Hit
GCTTCCCATGTACTCTGCGTTGATA133450.21458979018923457No Hit
TATCAACGCAGAGTACATGGGAAGC131130.21085919211325838No Hit
GGTATCAACGCAGAGTACATGGGAA119500.1921579612410156No Hit
ACTCTGCGTTGATACCACTGCTTCC118800.19103234975257452No Hit
GTGGTATCAACGCAGAGTACATGGG83320.13397992745273157No Hit
GTATCAACGCAGAGTACTTTTTTTT79780.1282875493540437No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN72090.11592190314531226No Hit
CAGTGGTATCAACGCAGAGTACATG64250.10331505447477199No Hit
ATTCCATTCCATTCCATTCCATTCC63340.10185175953979857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT802.8712966E-511.8743158
GTCTAGG6350.09.5842161
CTAATAC8550.09.5585483
TATTCCG1901.0477379E-99.5037415
CTAGTAC3900.09.2593093
ATACCGA1054.5013998E-49.0465876
GTATTAG7800.09.0216821
TCGTGCA950.00182601818.9994819
GTCTTAG6100.08.88577651
CCTACAC6850.08.8786933
TAGACTG8200.08.8083465
AGACCGT1304.3792737E-58.768236
GGAACGA1856.6118446E-88.72767815
TAGACAG9550.08.6578595
CTAAGAC6050.08.6390693
GAAGCGT1000.00290994118.5490246
TTAGACA7250.08.520944
GTCTAAT5700.08.5083561
TAGGACT6850.08.4635274
GTTCTAG6800.08.3905941
Produced by FastQC (version 0.11.5)