##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294978_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6218842 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.091953936118653 33.0 33.0 33.0 27.0 33.0 2 31.039701603610446 33.0 33.0 33.0 27.0 33.0 3 31.020752577409105 33.0 33.0 33.0 27.0 33.0 4 34.457410077310215 37.0 37.0 37.0 27.0 37.0 5 34.79564362625711 37.0 37.0 37.0 33.0 37.0 6 34.83822711688124 37.0 37.0 37.0 33.0 37.0 7 34.67513164026357 37.0 37.0 37.0 27.0 37.0 8 34.587576754643386 37.0 37.0 37.0 27.0 37.0 9 34.50154257014409 37.0 37.0 37.0 27.0 37.0 10 34.39202137632697 37.0 37.0 37.0 27.0 37.0 11 34.62503678337543 37.0 37.0 37.0 27.0 37.0 12 34.51897571927378 37.0 37.0 37.0 27.0 37.0 13 34.45410737240149 37.0 37.0 37.0 27.0 37.0 14 35.78696210645004 37.0 37.0 40.0 27.0 40.0 15 35.95894637619029 40.0 37.0 40.0 27.0 40.0 16 36.05171831025776 40.0 37.0 40.0 27.0 40.0 17 35.990827713583975 40.0 37.0 40.0 27.0 40.0 18 35.92930532726189 37.0 37.0 40.0 27.0 40.0 19 35.996337742621535 40.0 37.0 40.0 27.0 40.0 20 35.99005281047501 40.0 37.0 40.0 27.0 40.0 21 35.98390472052514 40.0 37.0 40.0 27.0 40.0 22 35.90706613867984 37.0 37.0 40.0 27.0 40.0 23 35.817321617111354 37.0 37.0 40.0 27.0 40.0 24 35.864543913481 37.0 37.0 40.0 27.0 40.0 25 34.687084026254404 37.0 33.0 40.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36944666384639646 1101 2 0.3424674234669851 1101 3 0.34425747562690034 1101 4 0.4446073471416838 1101 5 0.25466982507825975 1101 6 0.24425492106211522 1101 7 0.23617653706001107 1101 8 0.36016434882265713 1101 9 0.3925653451526543 1101 10 0.5750627459962914 1101 11 0.4957894503150726 1101 12 0.5256234943416445 1101 13 0.5087427758016716 1101 14 0.9489763385333561 1101 15 0.7961836275547896 1101 16 0.7650973923593654 1101 17 0.7398822531724392 1101 18 0.7572824023761058 1101 19 0.6091897491908327 1101 20 0.6028706835125988 1101 21 0.6098431641030118 1101 22 0.7737626759526179 1101 23 0.9149861040968972 1101 24 0.8103307694814887 1101 25 0.8485642427529854 1102 1 0.30880055825476305 1102 2 0.37637606199155016 1102 3 0.37231341420736186 1102 4 0.41437922239955327 1102 5 0.23246051182402283 1102 6 0.21620714676809172 1102 7 0.17643953448252603 1102 8 0.32833823509978544 1102 9 0.36600603447890734 1102 10 0.5144002146379023 1102 11 0.3792321364897191 1102 12 0.49423847077260774 1102 13 0.44343941861792757 1102 14 0.7885279544643282 1102 15 0.7379339101217184 1102 16 0.6996191104790128 1102 17 0.6936760853067412 1102 18 0.7616816042004757 1102 19 0.58949225398419 1102 20 0.6717389545004266 1102 21 0.6684603230226926 1102 22 0.7133793583306769 1102 23 0.755498615234103 1102 24 0.727811301303106 1102 25 0.8610715893958556 1103 1 0.40571813118411626 1103 2 0.3628547965736324 1103 3 0.3298793352147129 1103 4 0.3738977630141349 1103 5 0.2732826759031539 1103 6 0.2199045714927479 1103 7 0.18176128006656 1103 8 0.37402299367548864 1103 9 0.4307758689675012 1103 10 0.5324144226516694 1103 11 0.3765590663052123 1103 12 0.3974787429469089 1103 13 0.4289954058590908 1103 14 0.7340555198146177 1103 15 0.6913677692752884 1103 16 0.6471521249831156 1103 17 0.6019415640153056 1103 18 0.6554468679977816 1103 19 0.5886931489951266 1103 20 0.5616839616000036 1103 21 0.6343422915667176 1103 22 0.6157228152852667 1103 23 0.773628111547815 1103 24 0.6579166551933184 1103 25 0.8917697985314277 1104 1 0.27066913681780136 1104 2 0.24088162341212183 1104 3 0.20835066382736045 1104 4 0.20029826954989005 1104 5 0.1183273601383732 1104 6 0.05273410840851511 1104 7 0.10270654070812668 1104 8 0.24976028442348053 1104 9 0.2780512801030355 1104 10 0.3906743571917701 1104 11 0.2588943945045301 1104 12 0.4004444113483743 1104 13 0.3025938590664268 1104 14 0.5193145247118025 1104 15 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-0.03192280046958729 2214 16 -0.17703912833755453 2214 17 -0.1807013442243388 2214 18 -0.04453841787415058 2214 19 -0.759984870024013 2214 20 -0.3339263120156488 2214 21 -0.2596878051367568 2214 22 -0.5615328291173256 2214 23 -1.7924249742518157 2214 24 -1.216645103406286 2214 25 -1.5555900655457293 2215 1 -0.3459614392770014 2215 2 -0.1867164285593148 2215 3 -1.1304030523856028 2215 4 -0.7903698701599282 2215 5 -0.2750640833272584 2215 6 -0.24813860879306304 2215 7 -0.041601674297332636 2215 8 -0.04425223137090484 2215 9 -0.24079971579892145 2215 10 -1.2570970816984968 2215 11 -0.5012820561817506 2215 12 -0.4926131118999919 2215 13 -0.46815548062574663 2215 14 -0.7115440048117065 2215 15 -0.841134721685016 2215 16 -0.6012234443090634 2215 17 -0.4499381866145953 2215 18 -0.38088055291885325 2215 19 -0.9191657612169521 2215 20 -1.3261696398031688 2215 21 -0.672100990710284 2215 22 -0.2756371007781908 2215 23 -0.6196915062567285 2215 24 -1.6501726538546393 2215 25 -0.7006537115201183 2216 1 -0.5786669472189097 2216 2 -0.4896181086350353 2216 3 -1.4726529451624586 2216 4 -0.6471869511762591 2216 5 -0.4819220662750823 2216 6 -0.46601118148296194 2216 7 -0.27870088158555006 2216 8 -0.4381220717193415 2216 9 -0.21246193332139995 2216 10 -0.2713469911134254 2216 11 -0.42029299663963116 2216 12 -0.3059142351548374 2216 13 -1.0757651809925264 2216 14 -0.6800033469052948 2216 15 -1.1435742330432745 2216 16 -0.8407747801615244 2216 17 -1.7778271108337194 2216 18 -0.8494471181548917 2216 19 -0.6063675699604474 2216 20 -0.5552809078462388 2216 21 -0.2785609077417561 2216 22 -0.3278611490803627 2216 23 -0.24637440120763188 2216 24 -0.42825143698289025 2216 25 -0.3739502038444158 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8053.0 3 853.0 4 1063.0 5 979.0 6 646.0 7 532.0 8 1150.0 9 2223.0 10 4107.0 11 7122.0 12 11373.0 13 16942.0 14 22354.0 15 27994.0 16 31436.0 17 33419.0 18 34593.0 19 35279.0 20 36752.0 21 39792.0 22 42752.0 23 45676.0 24 48097.0 25 51197.0 26 53807.0 27 60510.0 28 68564.0 29 80353.0 30 97073.0 31 123527.0 32 162967.0 33 228561.0 34 329848.0 35 521051.0 36 932472.0 37 3055725.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.90907342963594 20.50810341373462 13.49837712352282 23.084446033106616 2 14.831738982580678 21.051793054598626 40.1493190576075 23.967148905213197 3 18.706988779541515 26.516002526375487 29.212473334493406 25.56453535958959 4 12.363973265063864 18.06409899803617 37.4519718417447 32.119955895155265 5 12.867565571784608 38.96685500290879 34.748076969885815 13.417502455420793 6 31.655535285107355 37.51886491648362 17.476690640504096 13.348909157904929 7 26.26141711783065 33.19124433614933 22.718139208765457 17.829199337254558 8 24.799865021593163 36.956091891674994 20.8965943897263 17.347448697005547 9 24.491577769960244 17.573161410591993 21.643939502593135 36.29132131685463 10 14.921327417511844 30.822842849844395 33.25958970672847 20.996240025915295 11 31.69124590804734 26.348702925324208 22.443710827664848 19.516340338963605 12 21.41522137799915 28.36509495938507 30.9609667724734 19.258716890142377 13 26.82669059499642 23.855020235457474 26.067601076968227 23.25068809257788 14 20.428335017217513 24.247626045579278 27.743917471950034 27.58012146525317 15 24.291883166945862 29.91210661988558 23.521126329393216 22.27488388377534 16 21.114815601353595 31.338311729953617 26.62563512260642 20.921237546086367 17 20.80197141423578 30.525629899653673 28.301247150881697 20.371151535228847 18 21.00031356377639 29.711392419748783 29.152359446567992 20.135934569906837 19 21.210173974231044 29.41532663814549 29.496085253512284 19.878414134111186 20 22.504609754698535 28.658140849354957 28.309355949113208 20.527893446833303 21 21.94854688028424 29.3868422405194 28.107031563303437 20.55757931589292 22 21.098296537938374 29.040530093443728 28.0456703524901 21.8155030161278 23 20.407066584382168 29.58532800739916 28.452605577406782 21.554999830811894 24 21.087940389689063 29.658292427472173 28.089631417764195 21.164135765074573 25 21.73903654865166 28.26352570195192 26.977195325036796 23.020242424359623 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13561.0 1 13561.0 2 11910.0 3 10259.0 4 10259.0 5 10259.0 6 13160.5 7 16062.0 8 16062.0 9 16062.0 10 20700.5 11 25339.0 12 25339.0 13 25339.0 14 39140.0 15 52941.0 16 52941.0 17 52941.0 18 84364.5 19 115788.0 20 115788.0 21 115788.0 22 167482.5 23 219177.0 24 219177.0 25 219177.0 26 286149.5 27 353122.0 28 353122.0 29 353122.0 30 423653.5 31 494185.0 32 494185.0 33 494185.0 34 553729.5 35 613274.0 36 613274.0 37 613274.0 38 652659.0 39 692044.0 40 692044.0 41 692044.0 42 698204.0 43 704364.0 44 704364.0 45 704364.0 46 788862.5 47 873361.0 48 873361.0 49 873361.0 50 795963.5 51 718566.0 52 718566.0 53 718566.0 54 613646.5 55 508727.0 56 508727.0 57 508727.0 58 434063.5 59 359400.0 60 359400.0 61 359400.0 62 296385.0 63 233370.0 64 233370.0 65 233370.0 66 179248.0 67 125126.0 68 125126.0 69 125126.0 70 90860.0 71 56594.0 72 56594.0 73 56594.0 74 39503.5 75 22413.0 76 22413.0 77 22413.0 78 14989.0 79 7565.0 80 7565.0 81 7565.0 82 5028.0 83 2491.0 84 2491.0 85 2491.0 86 1619.0 87 747.0 88 747.0 89 747.0 90 501.0 91 255.0 92 255.0 93 255.0 94 163.0 95 71.0 96 71.0 97 71.0 98 55.5 99 40.0 100 40.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3112476567180835 2 0.18001743732997236 3 0.198139782293874 4 0.21914047663536074 5 0.24475617807945596 6 0.19641920473297117 7 0.1919006786150862 8 0.2155546000364698 9 0.21577972233415804 10 0.2253152596576662 11 0.2002462837936709 12 0.20699995272431748 13 0.21941383942541073 14 0.20827028568984385 15 0.1985096260686475 16 0.20196686135457373 17 0.20158093741567967 18 0.20531153549165582 19 0.20410552318261183 20 0.2000050813318621 21 0.1977699385191005 22 0.201645258072162 23 0.2047326495833147 24 0.22095753518098707 25 0.2148309926510434 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 6218842.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.4575470698938 #Duplication Level Percentage of deduplicated Percentage of total 1 86.6496055879977 62.784178754800756 2 9.86200157656431 14.291528868745509 3 1.9970478047694002 4.3410355594472065 4 0.599868435896499 1.738599817188566 5 0.268995972744076 0.9745394178357878 6 0.14354687368349264 0.6240632613994662 7 0.0889698778553038 0.4512577378752364 8 0.058664212708196745 0.3400531962897942 9 0.043622846301553934 0.28447239952958286 >10 0.2510534899954474 3.362504349941559 >50 0.020275523878348134 1.0068082857037233 >100 0.013439195726458497 1.8777516847859734 >500 0.0012753281182106275 0.6325315051657829 >1k 0.0010963070452664834 1.947309918871423 >5k 2.908569709773589E-4 1.316752420921616 >10k+ 2.4610974467314986E-4 4.026612821497991 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 55364 0.890262206372183 No Hit CCCATGTACTCTGCGTTGATACCAC 51714 0.8315696073320402 No Hit GAGTACATGGGAAGCAGTGGTATCA 25002 0.4020362633429182 No Hit CATGTACTCTGCGTTGATACCACTG 18756 0.3015995582457313 No Hit GCGTTGATACCACTGCTTCCCATGT 17003 0.27341103054234217 No Hit GTATCAACGCAGAGTACATGGGAAG 15860 0.2550314029525111 No Hit ACGCAGAGTACATGGGAAGCAGTGG 14395 0.231473962515851 No Hit GCTTCCCATGTACTCTGCGTTGATA 13345 0.21458979018923457 No Hit TATCAACGCAGAGTACATGGGAAGC 13113 0.21085919211325838 No Hit GGTATCAACGCAGAGTACATGGGAA 11950 0.1921579612410156 No Hit ACTCTGCGTTGATACCACTGCTTCC 11880 0.19103234975257452 No Hit GTGGTATCAACGCAGAGTACATGGG 8332 0.13397992745273157 No Hit GTATCAACGCAGAGTACTTTTTTTT 7978 0.1282875493540437 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 7209 0.11592190314531226 No Hit CAGTGGTATCAACGCAGAGTACATG 6425 0.10331505447477199 No Hit ATTCCATTCCATTCCATTCCATTCC 6334 0.10185175953979857 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.216032824117416E-5 0.0 5 0.0 0.0 0.0 4.8240492361761244E-5 0.0 6 0.0 0.0 0.0 4.8240492361761244E-5 0.0 7 0.0 0.0 0.0 4.8240492361761244E-5 0.0 8 0.0 0.0 0.0 4.8240492361761244E-5 0.0 9 0.0 0.0 0.0 4.8240492361761244E-5 0.0 10 0.0 0.0 0.0 8.04008206029354E-5 0.0 11 0.0 0.0 0.0 8.04008206029354E-5 0.0 12 0.0 0.0 0.0 9.648098472352249E-5 0.0 13 0.0 0.0 0.0 9.648098472352249E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGCGT 80 2.8712966E-5 11.874315 8 GTCTAGG 635 0.0 9.584216 1 CTAATAC 855 0.0 9.558548 3 TATTCCG 190 1.0477379E-9 9.503741 5 CTAGTAC 390 0.0 9.259309 3 ATACCGA 105 4.5013998E-4 9.046587 6 GTATTAG 780 0.0 9.021682 1 TCGTGCA 95 0.0018260181 8.999481 9 GTCTTAG 610 0.0 8.8857765 1 CCTACAC 685 0.0 8.878693 3 TAGACTG 820 0.0 8.808346 5 AGACCGT 130 4.3792737E-5 8.76823 6 GGAACGA 185 6.6118446E-8 8.727678 15 TAGACAG 955 0.0 8.657859 5 CTAAGAC 605 0.0 8.639069 3 GAAGCGT 100 0.0029099411 8.549024 6 TTAGACA 725 0.0 8.52094 4 GTCTAAT 570 0.0 8.508356 1 TAGGACT 685 0.0 8.463527 4 GTTCTAG 680 0.0 8.390594 1 >>END_MODULE