FastQCFastQC Report
Wed 25 May 2016
SRR1294978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294978_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6218842
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC580550.933533928020683No Hit
GTACATGGGAAGCAGTGGTATCAAC528650.850077876234836No Hit
GAGTACATGGGAAGCAGTGGTATCA266740.4289222977525398No Hit
GTATCAACGCAGAGTACTTTTTTTT219860.35353848835522755No Hit
CATGTACTCTGCGTTGATACCACTG213280.34295774036388127No Hit
GCGTTGATACCACTGCTTCCCATGT185780.29873728903226676No Hit
GGTATCAACGCAGAGTACTTTTTTT176640.28404001902605014No Hit
ACGCAGAGTACATGGGAAGCAGTGG173250.27858884338917117No Hit
GTATCAACGCAGAGTACATGGGAAG156550.25173496930779077No Hit
GCTTCCCATGTACTCTGCGTTGATA145160.23341966237444203No Hit
TATCAACGCAGAGTACTTTTTTTTT143600.2309111567716305No Hit
ACTCTGCGTTGATACCACTGCTTCC139920.22499365637525442No Hit
TATCAACGCAGAGTACATGGGAAGC138830.2232409184861104No Hit
GGTATCAACGCAGAGTACATGGGAA129540.208302446018085No Hit
ACGCAGAGTACTTTTTTTTTTTTTT89110.1432903424785515No Hit
GTGGTATCAACGCAGAGTACATGGG88530.14235769295955744No Hit
CAGTGGTATCAACGCAGAGTACATG78430.12611672719776448No Hit
CATGGGAAGCAGTGGTATCAACGCA70120.11275411081355662No Hit
ACATGGGAAGCAGTGGTATCAACGC69270.11138729686330671No Hit
ATACCACTGCTTCCCATGTACTCTG64850.10427986432200723No Hit
GCAGTGGTATCAACGCAGAGTACAT63830.10263968758170733No Hit
ATTCCATTCCATTCCATTCCATTCC63060.10140151494442214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCCG1103.8253347E-812.0900516
TAGCCCG750.00265086910.1326945
ACGCCGA1252.5754434E-69.87945657
TAGGACC4050.09.8512314
CGCCGAG1451.6213198E-79.8270458
TGCACCG1252.7592228E-59.1194255
GACCGTG1151.112382E-49.0864577
TGACCGC950.00182610828.99943310
GTCGTAC850.0073676048.9518411
CTTACAC8950.08.915643
GTGTAGG6750.08.8772421
TCTGGAC6000.08.8661073
CTAATAC7300.08.7186293
CTAGGAC6150.08.6498613
ACTGTTC9050.08.6072618
TAGACTG9500.08.5994585
CGTTCAC1000.0029085988.5494610
TAACCCG2356.202754E-108.4888265
CCAGGAC11050.08.4248083
CTAGACA7150.08.3701014
Produced by FastQC (version 0.11.5)