FastQCFastQC Report
Wed 25 May 2016
SRR1294977_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294977_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5431468
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC543981.0015340235825747No Hit
CCCATGTACTCTGCGTTGATACCAC494480.9103984410844361No Hit
GAGTACATGGGAAGCAGTGGTATCA236310.4350757474774775No Hit
CATGTACTCTGCGTTGATACCACTG180030.33145735186141206No Hit
GCGTTGATACCACTGCTTCCCATGT162090.2984276074166321No Hit
GTATCAACGCAGAGTACATGGGAAG157880.29067648009709346No Hit
ACGCAGAGTACATGGGAAGCAGTGG140390.25847524094775115No Hit
GCTTCCCATGTACTCTGCGTTGATA138760.2554742106553882No Hit
TATCAACGCAGAGTACATGGGAAGC122980.22642129162870886No Hit
GGTATCAACGCAGAGTACATGGGAA118160.21754707935313253No Hit
ACTCTGCGTTGATACCACTGCTTCC112100.20638987470790585No Hit
GTGGTATCAACGCAGAGTACATGGG83150.1530893673680854No Hit
GTATCAACGCAGAGTACTTTTTTTT71970.1325056135836573No Hit
CAGTGGTATCAACGCAGAGTACATG65210.12005962292330546No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN64230.11825532250213018No Hit
ACATGGGAAGCAGTGGTATCAACGC56650.1042996110812031No Hit
GTACATGGTAAGCAGTGGTATCAAC56000.1031028812100154No Hit
ATACCACTGCTTCCCATGTACTCTG55950.10301082506607791No Hit
ATTCCATTCCATTCCATTCCATTCC55190.1016115716782277No Hit
GGTATCAACGCAGAGTACTTTTTTT55190.1016115716782277No Hit
GCAGTGGTATCAACGCAGAGTACAT54740.10078306638279008No Hit
CATGGGAAGCAGTGGTATCAACGCA54500.10034119689189No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCG701.0870792E-412.22003655
GTCTACG1053.4265468E-610.8687781
TCTAGCG700.001487392110.8615532
TAACGCT951.6372811E-410.0049864
CTAGGAC5600.09.6735713
GTACCGT1002.7615143E-49.4986856
GGACCGA800.00452367179.4986856
CGCGCCT800.0045284949.49745813
GTCTAGC3300.09.22199251
ACACCGA1356.8675527E-69.1468826
CTGTCGC4500.09.07702359
TAGGACT6400.09.0592014
CTAAGAC5400.08.9758673
GCACCGT1502.7102415E-68.8654396
TGTACCG1304.3489905E-58.773365
GTGCGTT1201.7706279E-48.70592311
ACCGAGC1107.155465E-48.6356468
GCTCGTT1107.16927E-48.63397311
TGTCGCC4300.08.61532510
TCTACAC5550.08.5620363
Produced by FastQC (version 0.11.5)