##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294977_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5431468
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.218949830874454	33.0	33.0	33.0	27.0	33.0
2	31.120913535714468	33.0	33.0	33.0	27.0	33.0
3	31.254739050289903	33.0	33.0	33.0	27.0	33.0
4	34.78582051850439	37.0	37.0	37.0	27.0	37.0
5	34.99067287149625	37.0	37.0	37.0	33.0	37.0
6	34.96129407372003	37.0	37.0	37.0	33.0	37.0
7	34.949868617471374	37.0	37.0	37.0	33.0	37.0
8	34.886021053608346	37.0	37.0	37.0	33.0	37.0
9	34.81586801211017	37.0	37.0	37.0	33.0	37.0
10	34.749403292074994	37.0	37.0	37.0	27.0	37.0
11	34.89751315850521	37.0	37.0	37.0	33.0	37.0
12	34.82201607373918	37.0	37.0	37.0	33.0	37.0
13	34.73037105254049	37.0	37.0	37.0	27.0	37.0
14	36.136313608033774	40.0	37.0	40.0	27.0	40.0
15	36.27739664488495	40.0	37.0	40.0	27.0	40.0
16	36.34402798654065	40.0	37.0	40.0	27.0	40.0
17	36.27882811792318	40.0	37.0	40.0	27.0	40.0
18	36.211486655173154	40.0	37.0	40.0	27.0	40.0
19	36.28589508398098	40.0	37.0	40.0	27.0	40.0
20	36.302338152411096	40.0	37.0	40.0	27.0	40.0
21	36.300523909926376	40.0	37.0	40.0	27.0	40.0
22	36.20368084650411	40.0	37.0	40.0	27.0	40.0
23	36.1049602059701	40.0	37.0	40.0	27.0	40.0
24	36.165586541244465	40.0	37.0	40.0	27.0	40.0
25	35.00106729893281	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.42062908221080875
1101	2	0.31460696528649024
1101	3	0.37788567378115445
1101	4	0.44491411126298175
1101	5	0.22312946197538963
1101	6	0.245857789731744
1101	7	0.3126250469886003
1101	8	0.36617448179774925
1101	9	0.41574865055341803
1101	10	0.5710830201226358
1101	11	0.4562559003245994
1101	12	0.45011067224792356
1101	13	0.4611289729521175
1101	14	0.8548309788204662
1101	15	0.8064578484651079
1101	16	0.7694503120220091
1101	17	0.6857818345436257
1101	18	0.7683226380446726
1101	19	0.6453481615700127
1101	20	0.6691963496461852
1101	21	0.6608721588309265
1101	22	0.7291468441336661
1101	23	0.9017211796763789
1101	24	0.7602123673271919
1101	25	0.8300214969778992
1102	1	0.4305999288909419
1102	2	0.31974822604149367
1102	3	0.3684680163223071
1102	4	0.49503004996880406
1102	5	0.2904069157303226
1102	6	0.1977873011013287
1102	7	0.32373986126064125
1102	8	0.33912137537862463
1102	9	0.3482782182574411
1102	10	0.5324583397442737
1102	11	0.39958214100211364
1102	12	0.4728370838881162
1102	13	0.3911039420433653
1102	14	0.8570431084730714
1102	15	0.7441557225933693
1102	16	0.7012987201349787
1102	17	0.5490458304571746
1102	18	0.7269734835772681
1102	19	0.6608792453995918
1102	20	0.7194559395349742
1102	21	0.7246907686796433
1102	22	0.7102918908642124
1102	23	0.7694896310540571
1102	24	0.8730226911803456
1102	25	0.9271664345699762
1103	1	0.32650407183547614
1103	2	0.3228911290004248
1103	3	0.33549122525410624
1103	4	0.4023857486128506
1103	5	0.17683328556859124
1103	6	0.0830912214263364
1103	7	0.11144576592570132
1103	8	0.3748740406034159
1103	9	0.3072116354830783
1103	10	0.4760009685817579
1103	11	0.2748836954295868
1103	12	0.3308517191228262
1103	13	0.3028037086713766
1103	14	0.6324790997757148
1103	15	0.603852635961367
1103	16	0.5626030077990407
1103	17	0.5796502530101932
1103	18	0.5537514015005627
1103	19	0.49840555371034867
1103	20	0.528411486633928
1103	21	0.5129389505330408
1103	22	0.49810226200519025
1103	23	0.5824425167177338
1103	24	0.5576096798430896
1103	25	0.6926376005372887
1104	1	0.28435831557650815
1104	2	0.26355427861888714
1104	3	0.3059462945537632
1104	4	0.3502990368774235
1104	5	0.22749548162940414
1104	6	0.14628357447900697
1104	7	0.24610252612291816
1104	8	0.4027102310996682
1104	9	0.36811059464272944
1104	10	0.5376973476367155
1104	11	0.3813584103568033
1104	12	0.4473713156099919
1104	13	0.39949720365320474
1104	14	0.7216967028008909
1104	15	0.643731820792425
1104	16	0.5322380467720507
1104	17	0.567676025976894
1104	18	0.6940714166170849
1104	19	0.598257752274975
1104	20	0.5924506632281279
1104	21	0.5533301022200803
1104	22	0.5872377596218783
1104	23	0.5700674152194196
1104	24	0.6539833527599512
1104	25	0.675473445880769
1105	1	0.25769337873450127
1105	2	0.22992951249428373
1105	3	0.15327627528522214
1105	4	0.25361262073280244
1105	5	0.18111263807691813
1105	6	0.03693286377706073
1105	7	0.13567482503661665
1105	8	0.278254893155534
1105	9	0.2551751907946027
1105	10	0.40977250890601624
1105	11	0.2302756021765191
1105	12	0.2912742654652476
1105	13	0.22305881178508713
1105	14	0.6064950347224425
1105	15	0.44254778296279085
1105	16	0.39423410928775837
1105	17	0.3913809846007297
1105	18	0.39233271012256665
1105	19	0.3371747346540346
1105	20	0.37469498273217283
1105	21	0.39358029071667033
1105	22	0.3565759969693332
1105	23	0.46648609639489536
1105	24	0.5168381219563472
1105	25	0.43089656254386455
1106	1	0.08913315084564388
1106	2	-0.10021023664076978
1106	3	-0.05394532028392618
1106	4	0.016061446862956075
1106	5	-0.44598833366892165
1106	6	-0.4097044949833162
1106	7	-0.6744212438150186
1106	8	-0.20835406178471771
1106	9	0.05995504977773436
1106	10	-0.22374646745329585
1106	11	-0.30066961830200256
1106	12	-0.6464499587253272
1106	13	-0.1253257293829364
1106	14	-0.2773077837635043
1106	15	-0.12415259353905839
1106	16	0.029479024494165174
1106	17	-0.14318475382080464
1106	18	-0.35319034368892943
1106	19	-0.5622299988574042
1106	20	-0.05306889160469552
1106	21	0.05986597260185533
1106	22	0.2621372969042426
1106	23	-0.49802913770341917
1106	24	0.08975751947109956
1106	25	0.19025417578725268
1107	1	-1.1909491920304056
1107	2	-1.2041448956169063
1107	3	-0.9059207782685732
1107	4	-0.999195573662071
1107	5	-1.0119751744926333
1107	6	-1.0459846356396625
1107	7	-1.0956280983470918
1107	8	-1.0177568516989552
1107	9	-1.1235224209610877
1107	10	-1.108463653710082
1107	11	-0.9943216774344918
1107	12	-1.012776605468538
1107	13	-0.9904057769882684
1107	14	-1.323742435066812
1107	15	-1.1443571767271052
1107	16	-1.1293771815714777
1107	17	-1.1329647235140854
1107	18	-1.2823609169716974
1107	19	-1.3121930283692151
1107	20	-1.3727175958650264
1107	21	-1.2807703657583147
1107	22	-1.240013082693892
1107	23	-0.9589129519078199
1107	24	-0.9135198101754369
1107	25	-0.8115236020462007
1108	1	-0.05744494824284274
1108	2	-0.22376918745746366
1108	3	-0.08841110856817025
1108	4	-0.24245887205446337
1108	5	-0.5432924449070313
1108	6	-0.1196923200097686
1108	7	-0.0756958760713502
1108	8	0.05812844528131933
1108	9	-0.2980211676768292
1108	10	-0.6345006455335636
1108	11	-0.19725752795916662
1108	12	0.01502448470859008
1108	13	0.03534722335646023
1108	14	-0.3491952519072967
1108	15	-0.06419419096700807
1108	16	-0.9385097336812009
1108	17	-0.8179870918709042
1108	18	-0.7103855242318389
1108	19	-0.0885726439893304
1108	20	-1.0766137167444327
1108	21	-0.2977992209144418
1108	22	-0.4422598994302902
1108	23	-0.22338160078989233
1108	24	-0.4552257149801804
1108	25	-1.3325383001626747
1109	1	0.12712445136167716
1109	2	0.07448556858571109
1109	3	0.20716338500751874
1109	4	0.14210352145814653
1109	5	0.04519876005680601
1109	6	0.18137771118450274
1109	7	0.14818983009811149
1109	8	0.22346276889980743
1109	9	0.2523547881493684
1109	10	0.2829412469056578
1109	11	0.14334782222431386
1109	12	0.26940352468246687
1109	13	0.2368940683624885
1109	14	0.508415991188123
1109	15	0.46506012701201627
1109	16	0.33963444689550215
1109	17	0.342362347574273
1109	18	0.33299321520585323
1109	19	0.30744085827465994
1109	20	0.3690115886831151
1109	21	0.3610701161134102
1109	22	0.3459388025064669
1109	23	0.4381056932209475
1109	24	0.4486767869452848
1109	25	0.4116430573210863
1110	1	-0.004204384993965249
1110	2	0.003842050182651491
1110	3	0.10091977445081568
1110	4	-1.0860627205239624
1110	5	-0.32617787832388956
1110	6	-0.0398685699416248
1110	7	0.06549143138054347
1110	8	0.23284630569566644
1110	9	0.2141590333400316
1110	10	0.07333527855746524
1110	11	0.08383759610251929
1110	12	0.14584173039990134
1110	13	0.12353496010032217
1110	14	0.2958308210273941
1110	15	0.1941337588020673
1110	16	0.16421699230043885
1110	17	0.21541776321542017
1110	18	0.3020571723342016
1110	19	0.17084108075265902
1110	20	0.22806290958446596
1110	21	0.204199543277781
1110	22	-0.3502903023794772
1110	23	0.038331559182708475
1110	24	0.20088344983633277
1110	25	0.28509714571135447
1111	1	-0.11596476328538685
1111	2	-0.2242156118565113
1111	3	-0.14603508978612467
1111	4	-0.13728696431346776
1111	5	-0.18903098654065786
1111	6	-0.08530142568058352
1111	7	-0.0961505289761817
1111	8	0.08787745395597568
1111	9	-0.004816640452695253
1111	10	0.03924389696791053
1111	11	-0.11173271265833051
1111	12	0.04332756740540589
1111	13	-0.07196330596472933
1111	14	-0.24601040721789502
1111	15	-0.09123069694162211
1111	16	-0.14661870284244571
1111	17	-0.07422789262864171
1111	18	-0.09231052662735806
1111	19	-0.06216902880679953
1111	20	-0.08081475700139862
1111	21	-0.15877994133221307
1111	22	-0.1056845666229691
1111	23	0.04361813487232524
1111	24	0.028665680787831604
1111	25	-0.11915504988672154
1112	1	-0.1800466011725561
1112	2	-0.49195336737164297
1112	3	-0.22054991618491115
1112	4	-0.30753685912914364
1112	5	-0.1538270601619658
1112	6	-0.2041480263677684
1112	7	-0.18105354255717288
1112	8	-0.035948667913089594
1112	9	-0.1492264096066407
1112	10	0.11570069116765325
1112	11	-0.07846203661234341
1112	12	0.0036145273463077388
1112	13	-0.10497928987540917
1112	14	-0.35062415892978294
1112	15	-0.28052553486502774
1112	16	-0.2613779990795564
1112	17	-0.29965525456337616
1112	18	-0.23120810500249434
1112	19	-0.25015749922535235
1112	20	-1.0759280284259845
1112	21	-1.4075187044833513
1112	22	-1.9030430781742922
1112	23	-0.6805503859029756
1112	24	-0.49454883384071024
1112	25	-1.6663286542504068
1113	1	-0.2356487282741888
1113	2	-0.17220405842173747
1113	3	-0.26131757289099866
1113	4	-0.562500490334088
1113	5	-0.30253018590084224
1113	6	-0.18389692692103665
1113	7	-0.21091700911378553
1113	8	-0.09408285415055673
1113	9	-0.24361654446351366
1113	10	-0.14122485597636825
1113	11	-0.27859304595387613
1113	12	-0.25318957553525934
1113	13	-0.27775900932841324
1113	14	-0.56201455602028
1113	15	-0.45793733193919195
1113	16	-0.4728409403091334
1113	17	-0.4079829993111801
1113	18	-0.9026331298287147
1113	19	-1.1255100100048736
1113	20	-1.3409339179353523
1113	21	-1.0662017543034423
1113	22	-0.5168750423041644
1113	23	-2.1033935143071787
1113	24	-1.121020136454021
1113	25	-0.5375182061748163
1114	1	-0.3450097716080869
1114	2	-0.4708688440059845
1114	3	-0.37685740601681417
1114	4	-0.5378516371527411
1114	5	-0.6210123150125426
1114	6	-0.4739889209914807
1114	7	-0.48494333256513045
1114	8	-0.14321738069907752
1114	9	-1.12764613857302
1114	10	-0.3731164868241805
1114	11	-0.3100605959201914
1114	12	-0.4284811150629082
1114	13	-0.44460151674691417
1114	14	-0.7821097743422598
1114	15	-1.371664693829544
1114	16	-1.2362307394067855
1114	17	-1.6811427042780451
1114	18	-1.8391531303775182
1114	19	-1.7136899855869174
1114	20	-0.9347964855250837
1114	21	-0.6869620047700806
1114	22	-0.5472366265778916
1114	23	-0.31067435044963077
1114	24	-0.9898871479824294
1114	25	-0.6381215124651902
1115	1	-0.760915234672769
1115	2	-0.5155922237886443
1115	3	-0.5151836485268362
1115	4	-0.7207604212534378
1115	5	-0.4412433429146887
1115	6	-0.4232340582452707
1115	7	-0.3814273894999687
1115	8	-0.21365563242885344
1115	9	-0.5558744248489305
1115	10	-0.28003049603588437
1115	11	-0.46306390922220686
1115	12	-0.6177253897964121
1115	13	-0.429463018089983
1115	14	-0.4124147190547873
1115	15	-1.6486349899165234
1115	16	-0.9178067657681481
1115	17	-0.7017653645349142
1115	18	-2.3380669387100994
1115	19	-1.1972019679220551
1115	20	-0.9292619120729952
1115	21	-0.9325344753352311
1115	22	-0.7450061639748142
1115	23	-0.39944653617821047
1115	24	-0.3891523846734444
1115	25	-0.4896586334764521
1116	1	-1.6162950676883838
1116	2	-0.8664795194326871
1116	3	-1.6896611815800178
1116	4	-1.0345069911774658
1116	5	-1.0408067359427164
1116	6	-0.5735244041517475
1116	7	-1.3078399336785225
1116	8	-3.1545680116879744
1116	9	-1.6445532060096113
1116	10	-0.8378469942616533
1116	11	-0.6620103475021679
1116	12	-0.84374121651188
1116	13	-1.9003371465246701
1116	14	-1.7292117167673808
1116	15	-1.6047542460115167
1116	16	-1.084038047399602
1116	17	-1.7092600547852967
1116	18	-2.6809756179110877
1116	19	-1.4107495835605022
1116	20	-1.0279540305612898
1116	21	-0.8434230837205021
1116	22	-0.6291503882735299
1116	23	-0.6049273653497451
1116	24	-0.6786954142375023
1116	25	-0.6127607043481689
1201	1	0.4278189652435387
1201	2	0.35728553340585023
1201	3	0.4290412022980874
1201	4	0.4404254246495185
1201	5	0.29969461574074074
1201	6	0.20664271307607862
1201	7	0.2513883779459576
1201	8	0.3462921505140386
1201	9	0.34266724127473225
1201	10	0.5309168297737372
1201	11	0.4496878584452375
1201	12	0.51347185795359
1201	13	0.4744478129248222
1201	14	0.9392571945130328
1201	15	0.8180769164362616
1201	16	0.635057553485133
1201	17	0.6998085124302378
1201	18	0.5947678079920848
1201	19	0.6332415690568496
1201	20	0.6541545956367969
1201	21	0.641093996643221
1201	22	0.7211813885758218
1201	23	0.7658025437188272
1201	24	0.8071733495865345
1201	25	0.9029679759172495
1202	1	0.2777633396314272
1202	2	0.25694664853433125
1202	3	0.23806768270081236
1202	4	0.36505424556225563
1202	5	0.2011169035208269
1202	6	-0.05751375200260611
1202	7	0.023797134736405212
1202	8	0.08814748585039212
1202	9	0.17917983327328102
1202	10	0.3404273878407267
1202	11	0.13463932743241713
1202	12	0.1681231402163874
1202	13	0.18231215915884036
1202	14	0.564628703070845
1202	15	0.36953228550063244
1202	16	0.2817282657248228
1202	17	0.40673658266261725
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2210	22	0.5660536146066164
2210	23	0.6415748572748967
2210	24	0.5258284496944654
2210	25	0.5639031022971395
2211	1	0.021777262191701396
2211	2	-0.12899653986570314
2211	3	-0.007237248296313226
2211	4	-5.430790797120721E-4
2211	5	0.03613031735827121
2211	6	0.02951535182292986
2211	7	0.06749432537554156
2211	8	0.0562989967029921
2211	9	0.031222359972197467
2211	10	0.14968783766737204
2211	11	-0.0055038739793573654
2211	12	0.0038851808184361403
2211	13	-0.06422798341005631
2211	14	-0.14829653906947726
2211	15	-0.1017351527139354
2211	16	-0.09374917020366524
2211	17	-0.15518743330206064
2211	18	-0.0680973025185807
2211	19	-0.05902589923522328
2211	20	0.011732313801907424
2211	21	-0.001201267869234357
2211	22	-0.044694071610400954
2211	23	0.0991104773147029
2211	24	0.1758699751969388
2211	25	-0.002885521229664789
2212	1	0.11973580310533904
2212	2	0.13836761338260573
2212	3	0.1288662913353953
2212	4	0.06989244995471466
2212	5	0.28947705648323563
2212	6	0.1928070685518506
2212	7	0.181466871989997
2212	8	0.2641245689208347
2212	9	0.16641304060099316
2212	10	0.37738717759970797
2212	11	0.2123185562560721
2212	12	0.226513084638583
2212	13	0.16283169917431195
2212	14	0.2380666539890015
2212	15	0.21080525317992027
2212	16	0.1150356115416713
2212	17	0.2035634904407715
2212	18	0.20164973019322474
2212	19	0.27535706569020135
2212	20	0.11866586464420692
2212	21	0.21017087355516395
2212	22	-0.35015898446437177
2212	23	0.22134148591619862
2212	24	0.20854216840120898
2212	25	0.35562739106126884
2213	1	-0.03834679124307172
2213	2	-0.6153440947968996
2213	3	-0.12276726035025476
2213	4	0.016924677202972305
2213	5	0.07498456023253652
2213	6	0.04897682134417636
2213	7	-0.002206083862695607
2213	8	0.14073936957849753
2213	9	-0.2087019812817772
2213	10	-1.6333783219654308
2213	11	-0.4976829064394295
2213	12	-0.06260096494619205
2213	13	-0.06151023654841481
2213	14	0.03446820456761657
2213	15	-0.01596881134740613
2213	16	0.052251853166829676
2213	17	-0.0742636844994351
2213	18	0.035366548703059664
2213	19	-0.013230632008877308
2213	20	0.05200987181081018
2213	21	0.006210234023420469
2213	22	0.11551792491466273
2213	23	-0.8825093118597778
2213	24	-1.5260399831935274
2213	25	-1.3068050412071344
2214	1	0.05346607577153506
2214	2	-0.14967886699519184
2214	3	0.04153342686610628
2214	4	0.005601256381538633
2214	5	0.08294433025633907
2214	6	0.09200903294718898
2214	7	0.052598695291088404
2214	8	0.04326937540565723
2214	9	-0.22766158128673908
2214	10	0.02115225254544839
2214	11	-0.040185346916253195
2214	12	-0.05705813677641913
2214	13	0.06640934934372922
2214	14	0.07543494911233495
2214	15	-0.012585866438413973
2214	16	-0.14518850063767985
2214	17	-0.09786050283079106
2214	18	0.1119496581718451
2214	19	-0.6812518630279598
2214	20	-0.34003750876008354
2214	21	-0.16866182415319741
2214	22	-0.3181690432898918
2214	23	-1.6591869528632088
2214	24	-1.1705118863060449
2214	25	-1.5413202073397656
2215	1	-0.35258968144047387
2215	2	-0.16600425293049526
2215	3	-1.1549080899148265
2215	4	-0.8472890473371208
2215	5	-0.22274258924512935
2215	6	-0.1931017593372033
2215	7	-0.15971485456439893
2215	8	-0.053246228516549365
2215	9	-0.3420222241271418
2215	10	-1.3144186370110305
2215	11	-0.48432364964000385
2215	12	-0.42965976713768583
2215	13	-0.3871951233258599
2215	14	-0.7118533297187213
2215	15	-0.9686962958667209
2215	16	-0.5874353266848757
2215	17	-0.39542214467444836
2215	18	-0.31073370412335066
2215	19	-0.8685817138890926
2215	20	-1.2105169789554324
2215	21	-0.5385036047048288
2215	22	-0.25972033079656853
2215	23	-0.5455506100740877
2215	24	-1.677600421936475
2215	25	-0.6496895004066943
2216	1	-0.5321692115245362
2216	2	-0.4516497591962114
2216	3	-1.4047464287013582
2216	4	-0.721912860072635
2216	5	-0.5187701712222719
2216	6	-0.4793262968539338
2216	7	-0.25444310114296087
2216	8	-0.33299988110659484
2216	9	-0.1822712633695005
2216	10	-0.3049910457136562
2216	11	-0.37307012496793135
2216	12	-0.3334411230254659
2216	13	-1.0352077321250945
2216	14	-0.7222714558489685
2216	15	-1.1120719513239692
2216	16	-0.8837831040074846
2216	17	-1.8206302119039535
2216	18	-0.8457532792719604
2216	19	-0.52694680553126
2216	20	-0.5720623454965406
2216	21	-0.39222366598240654
2216	22	-0.44460714099830767
2216	23	-0.26973916484914895
2216	24	-0.45142947547746815
2216	25	-0.32040140287449503
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7138.0
3	793.0
4	945.0
5	888.0
6	525.0
7	401.0
8	554.0
9	1104.0
10	2275.0
11	4188.0
12	7435.0
13	11423.0
14	15918.0
15	20539.0
16	23813.0
17	25434.0
18	26362.0
19	27555.0
20	28259.0
21	30293.0
22	33150.0
23	35181.0
24	37630.0
25	40400.0
26	42803.0
27	48136.0
28	54599.0
29	64926.0
30	79495.0
31	100716.0
32	136654.0
33	192741.0
34	280734.0
35	448593.0
36	816822.0
37	2783046.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98835586773212	20.271465550120375	13.59243043672191	23.1477481454256
2	14.721357666403886	20.928729080198273	40.05241686030791	24.297496393089933
3	18.777726798687137	26.127368152482706	29.212384118654235	25.88252093017593
4	12.280899875741005	17.505793155827863	37.40968895394003	32.8036180144911
5	13.03088801307216	38.32421514723251	34.946816374602314	13.698080465093016
6	31.83025027524669	36.88298252294214	17.53826888280837	13.748498319002794
7	26.490082229180985	32.71979818570052	22.754968109097785	18.035151476020715
8	24.616483196114828	37.30141450075467	20.642282243273463	17.439820059857038
9	24.39731452975209	17.63328403636105	21.51747336256708	36.45192807131977
10	14.94208754270888	30.743861131897198	33.12251162816447	21.191539697229448
11	31.67766377356506	26.36584436656941	22.196014475039966	19.760477384825563
12	21.42416843926193	28.29923658939203	30.80638647207694	19.4702084992691
13	26.85429729760151	23.6427437796748	25.907323931135178	23.595634991588508
14	20.42437959401538	24.101805936170365	27.595817606738354	27.8779968630759
15	24.58133642482873	29.638857352011694	23.34642323846196	22.433382984697616
16	21.19306719258936	31.00897272575487	26.747080197368607	21.05087988428717
17	21.079105220812107	30.4284625518907	28.078992865137952	20.413439362159234
18	21.318656908212937	29.486242096809278	28.928819116768796	20.266281878208993
19	21.343079892896327	29.3004332521735	29.3182555746878	20.038231280242368
20	22.478625682536386	28.481920269086803	28.278706746559806	20.760747301817002
21	21.972644377460437	29.1509817027151	28.175062553834668	20.70131136598979
22	21.308467286657407	28.766085837378625	27.75208217411005	22.17336470185392
23	20.50686074890021	29.534798937585744	28.278901453624172	21.679438859889878
24	21.165271552307146	29.705094912247386	27.83109677707455	21.29853675837092
25	21.890943833422728	27.986621180007198	26.739594410004702	23.382840576565368
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12473.0
1	12473.0
2	10542.5
3	8612.0
4	8612.0
5	8612.0
6	11038.0
7	13464.0
8	13464.0
9	13464.0
10	17304.0
11	21144.0
12	21144.0
13	21144.0
14	32700.0
15	44256.0
16	44256.0
17	44256.0
18	70340.5
19	96425.0
20	96425.0
21	96425.0
22	139318.5
23	182212.0
24	182212.0
25	182212.0
26	238903.5
27	295595.0
28	295595.0
29	295595.0
30	356186.5
31	416778.0
32	416778.0
33	416778.0
34	467704.0
35	518630.0
36	518630.0
37	518630.0
38	557362.5
39	596095.0
40	596095.0
41	596095.0
42	607571.5
43	619048.0
44	619048.0
45	619048.0
46	704470.5
47	789893.0
48	789893.0
49	789893.0
50	715486.0
51	641079.0
52	641079.0
53	641079.0
54	546530.5
55	451982.0
56	451982.0
57	451982.0
58	384892.0
59	317802.0
60	317802.0
61	317802.0
62	262850.5
63	207899.0
64	207899.0
65	207899.0
66	160881.0
67	113863.0
68	113863.0
69	113863.0
70	83402.5
71	52942.0
72	52942.0
73	52942.0
74	37033.5
75	21125.0
76	21125.0
77	21125.0
78	14189.0
79	7253.0
80	7253.0
81	7253.0
82	4713.0
83	2173.0
84	2173.0
85	2173.0
86	1353.0
87	533.0
88	533.0
89	533.0
90	330.5
91	128.0
92	128.0
93	128.0
94	81.5
95	35.0
96	35.0
97	35.0
98	32.0
99	29.0
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31580780739203473
2	0.18282350185990232
3	0.20151089907921765
4	0.2235491399378584
5	0.24930644901157475
6	0.20024052429287995
7	0.19595070798539177
8	0.21887268782583275
9	0.21970119312127034
10	0.23037970581802195
11	0.20441987322764305
12	0.21079015838811901
13	0.2227206346424208
14	0.21189483211536916
15	0.2026339840352553
16	0.2053404346670182
17	0.20585594907306828
18	0.20891221305179372
19	0.20802847406999359
20	0.20364660161856793
21	0.2013636092489176
22	0.20569024801398075
23	0.20915155902603128
24	0.2245985799787461
25	0.2183019397334201
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5431468.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.45851274665326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.17832783998574	59.1635997001226
2	10.563149787513392	14.674013483216134
3	2.3251419503406336	4.845027053865394
4	0.7815594517092495	2.1714382855525693
5	0.3386872542197453	1.1762356482175587
6	0.19154489957374252	0.7982654309159528
7	0.12167046896624628	0.5915734873708308
8	0.07768938957380347	0.4316951564793391
9	0.06287281597202235	0.393034706064963
>10	0.316616964617818	4.036045758163677
>50	0.025044969543966827	1.2029171547261888
>100	0.014433616610289584	1.8646973795142483
>500	0.001363037334316483	0.6529382035357754
>1k	0.001256127321607553	2.0882892042776304
>5k	3.474394719243191E-4	1.441683255823518
>10k+	2.939872454744239E-4	4.468546092153356
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	54398	1.0015340235825747	No Hit
CCCATGTACTCTGCGTTGATACCAC	49448	0.9103984410844361	No Hit
GAGTACATGGGAAGCAGTGGTATCA	23631	0.4350757474774775	No Hit
CATGTACTCTGCGTTGATACCACTG	18003	0.33145735186141206	No Hit
GCGTTGATACCACTGCTTCCCATGT	16209	0.2984276074166321	No Hit
GTATCAACGCAGAGTACATGGGAAG	15788	0.29067648009709346	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	14039	0.25847524094775115	No Hit
GCTTCCCATGTACTCTGCGTTGATA	13876	0.2554742106553882	No Hit
TATCAACGCAGAGTACATGGGAAGC	12298	0.22642129162870886	No Hit
GGTATCAACGCAGAGTACATGGGAA	11816	0.21754707935313253	No Hit
ACTCTGCGTTGATACCACTGCTTCC	11210	0.20638987470790585	No Hit
GTGGTATCAACGCAGAGTACATGGG	8315	0.1530893673680854	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7197	0.1325056135836573	No Hit
CAGTGGTATCAACGCAGAGTACATG	6521	0.12005962292330546	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	6423	0.11825532250213018	No Hit
ACATGGGAAGCAGTGGTATCAACGC	5665	0.1042996110812031	No Hit
GTACATGGTAAGCAGTGGTATCAAC	5600	0.1031028812100154	No Hit
ATACCACTGCTTCCCATGTACTCTG	5595	0.10301082506607791	No Hit
ATTCCATTCCATTCCATTCCATTCC	5519	0.1016115716782277	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5519	0.1016115716782277	No Hit
GCAGTGGTATCAACGCAGAGTACAT	5474	0.10078306638279008	No Hit
CATGGGAAGCAGTGGTATCAACGCA	5450	0.10034119689189	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.841122878750275E-5	0.0	0.0	0.0	0.0
2	1.841122878750275E-5	0.0	0.0	0.0	0.0
3	1.841122878750275E-5	0.0	0.0	0.0	0.0
4	1.841122878750275E-5	1.841122878750275E-5	0.0	0.0	0.0
5	1.841122878750275E-5	1.841122878750275E-5	0.0	0.0	0.0
6	1.841122878750275E-5	1.841122878750275E-5	0.0	0.0	0.0
7	1.841122878750275E-5	1.841122878750275E-5	0.0	0.0	0.0
8	3.68224575750055E-5	1.841122878750275E-5	0.0	1.841122878750275E-5	0.0
9	3.68224575750055E-5	1.841122878750275E-5	0.0	3.68224575750055E-5	0.0
10	5.5233686362508256E-5	1.841122878750275E-5	0.0	3.68224575750055E-5	0.0
11	5.5233686362508256E-5	1.841122878750275E-5	0.0	5.5233686362508256E-5	0.0
12	5.5233686362508256E-5	1.841122878750275E-5	0.0	7.3644915150011E-5	0.0
13	5.5233686362508256E-5	1.841122878750275E-5	0.0	7.3644915150011E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTACCG	70	1.0870792E-4	12.2200365	5
GTCTACG	105	3.4265468E-6	10.868778	1
TCTAGCG	70	0.0014873921	10.861553	2
TAACGCT	95	1.6372811E-4	10.004986	4
CTAGGAC	560	0.0	9.673571	3
GTACCGT	100	2.7615143E-4	9.498685	6
GGACCGA	80	0.0045236717	9.498685	6
CGCGCCT	80	0.004528494	9.497458	13
GTCTAGC	330	0.0	9.2219925	1
ACACCGA	135	6.8675527E-6	9.146882	6
CTGTCGC	450	0.0	9.0770235	9
TAGGACT	640	0.0	9.059201	4
CTAAGAC	540	0.0	8.975867	3
GCACCGT	150	2.7102415E-6	8.865439	6
TGTACCG	130	4.3489905E-5	8.77336	5
GTGCGTT	120	1.7706279E-4	8.705923	11
ACCGAGC	110	7.155465E-4	8.635646	8
GCTCGTT	110	7.16927E-4	8.633973	11
TGTCGCC	430	0.0	8.615325	10
TCTACAC	555	0.0	8.562036	3
>>END_MODULE