FastQCFastQC Report
Wed 25 May 2016
SRR1294977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294977_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5431468
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC551321.0150478655126016No Hit
GTACATGGGAAGCAGTGGTATCAAC518680.9549536147501928No Hit
GAGTACATGGGAAGCAGTGGTATCA257990.4749912914887835No Hit
GTATCAACGCAGAGTACTTTTTTTT210670.3878693568663205No Hit
CATGTACTCTGCGTTGATACCACTG204550.3766016848483688No Hit
GCGTTGATACCACTGCTTCCCATGT174190.32070519424951044No Hit
ACGCAGAGTACATGGGAAGCAGTGG168910.31098406544970897No Hit
GGTATCAACGCAGAGTACTTTTTTT168480.3101923826118464No Hit
GTATCAACGCAGAGTACATGGGAAG153430.28248348328665474No Hit
GCTTCCCATGTACTCTGCGTTGATA150280.2766839462185914No Hit
TATCAACGCAGAGTACTTTTTTTTT139300.25646841700991335No Hit
ACTCTGCGTTGATACCACTGCTTCC132740.24439065092531154No Hit
TATCAACGCAGAGTACATGGGAAGC132570.24407766003592396No Hit
GGTATCAACGCAGAGTACATGGGAA124990.2301219486149969No Hit
GTGGTATCAACGCAGAGTACATGGG87030.16023292413763646No Hit
ACGCAGAGTACTTTTTTTTTTTTTT85740.1578578756240486No Hit
CAGTGGTATCAACGCAGAGTACATG75790.13953870298048338No Hit
ACATGGGAAGCAGTGGTATCAACGC68730.1265403754565064No Hit
CATGGGAAGCAGTGGTATCAACGCA66050.12160616614145568No Hit
GCAGTGGTATCAACGCAGAGTACAT62260.11462831043099213No Hit
ATACCACTGCTTCCCATGTACTCTG60980.11227167314619178No Hit
CCATGTACTCTGCGTTGATACCACT56540.10409708756454056No Hit
GGGAAGCAGTGGTATCAACGCAGAG56160.10339746087061545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAC9050.09.6569443
CACCGTT900.0011149059.4995817
ACGCCGA800.0045201589.499587
GCACCGT1651.0686381E-79.2114616
TCGTCTA1151.11273825E-49.08613814
CTGACCG2106.693881E-109.0470539
GGCCCGT950.00182617488.9993546
AGACCGA950.00182617488.9993546
TAGACAG8250.08.7510495
CCTGGAC8950.08.7034473
GTTCTAG5600.08.662011
GGACTGT8150.08.6251496
TACACTG13600.08.5216055
CTAGACT6050.08.4788874
CTAGTAC3500.08.4138373
TGGACTG8950.08.3850285
TAGGCCG1252.7471565E-48.3595545
GTGTTAG7300.08.3386091
TTACACT9150.08.3055684
TAGACGT1150.001111958.2604294
Produced by FastQC (version 0.11.5)