FastQCFastQC Report
Wed 25 May 2016
SRR1294976_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294976_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6647234
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC458470.6897154515697808No Hit
CCCATGTACTCTGCGTTGATACCAC426110.6410335486910796No Hit
GAGTACATGGGAAGCAGTGGTATCA204490.30763171568805914No Hit
CATGTACTCTGCGTTGATACCACTG152010.2286815839490531No Hit
GCGTTGATACCACTGCTTCCCATGT140860.21190768972477875No Hit
GTATCAACGCAGAGTACATGGGAAG131770.19823282887288157No Hit
ACGCAGAGTACATGGGAAGCAGTGG118100.17766788411540801No Hit
GCTTCCCATGTACTCTGCGTTGATA107710.16203732259162232No Hit
TATCAACGCAGAGTACATGGGAAGC106080.1595851748261006No Hit
GGTATCAACGCAGAGTACATGGGAA101140.15215351227292434No Hit
ACTCTGCGTTGATACCACTGCTTCC98100.14758018147096974No Hit
GTATCAACGCAGAGTACTTTTTTTT76950.11576243592447626No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN76720.11541642734406521No Hit
GTGGTATCAACGCAGAGTACATGGG72450.10899270282947765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCG1104.9451046E-711.232855
ATCGTCG600.005883435311.0822339
TAGATCG750.00263862910.1383685
AACCGCT856.6054764E-410.057757
TATTCCG3250.09.9433995
GTCTAGG8000.08.9157211
GTGCGCT1201.7694043E-48.7065511
GTGTAGG7450.08.6803611
TACACCG1000.00289394928.5542485
GTATTAG8900.08.5484061
GTCTCGC5400.08.45342351
CTAGGAC6650.08.4321863
CGCAAAA2606.002665E-118.407452
AGCACCG1701.6026006E-68.3865185
CTAGACA8050.08.3832984
TAGGACC4100.08.3458594
TAGACTG9800.08.3408775
CCTAGAC6650.08.2892683
TAGACAG11050.08.2574945
TGGGCCG2451.3860699E-98.1469035
Produced by FastQC (version 0.11.5)