FastQCFastQC Report
Wed 25 May 2016
SRR1294976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294976_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6647234
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC476040.7161474983429198No Hit
GTACATGGGAAGCAGTGGTATCAAC440980.6634037556072195No Hit
GAGTACATGGGAAGCAGTGGTATCA217050.32652679294876635No Hit
GTATCAACGCAGAGTACTTTTTTTT209200.31471736966082436No Hit
CATGTACTCTGCGTTGATACCACTG171650.25822770794589145No Hit
GGTATCAACGCAGAGTACTTTTTTT166820.2509615277572596No Hit
GCGTTGATACCACTGCTTCCCATGT150930.22705684800625342No Hit
ACGCAGAGTACATGGGAAGCAGTGG140550.21144133033378998No Hit
TATCAACGCAGAGTACTTTTTTTTT134780.20276102812086952No Hit
GTATCAACGCAGAGTACATGGGAAG130340.19608155813380423No Hit
GCTTCCCATGTACTCTGCGTTGATA116960.17595288506467502No Hit
ACTCTGCGTTGATACCACTGCTTCC114190.1717857382484203No Hit
TATCAACGCAGAGTACATGGGAAGC113850.17127424730346488No Hit
GGTATCAACGCAGAGTACATGGGAA106970.1609240775937781No Hit
ACGCAGAGTACTTTTTTTTTTTTTT81690.12289322145120814No Hit
GTGGTATCAACGCAGAGTACATGGG73690.11085814039343282No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGA802.8713841E-511.8743097
AACGCCG802.8718263E-511.874136
CGTCCGC752.0768872E-411.39933710
AGGACCG1354.7457434E-911.2584355
CCGTTCA1451.2223609E-911.1372839
GACGCGT700.001493428810.8563476
GTCCGCA803.776211E-410.68687711
AGACCGT1752.3646862E-1010.313536
TAGACAG11500.010.1601265
AACCGAC750.002650803210.1327447
ACGCGTG750.002650803210.1327447
ACCGTTC951.6466308E-49.9994188
CGCCGAG951.6466308E-49.9994188
GTGTAGG8200.09.9751411
GTCTTAG6200.09.8179921
ACCGACC900.00111511789.4994488
CGCGTGC800.00452090759.4994478
GTGCGCT1106.848115E-59.49944711
TGTCCGC800.00452090759.49944710
GCACCGT2201.6370905E-119.4993046
Produced by FastQC (version 0.11.5)