FastQCFastQC Report
Wed 25 May 2016
SRR1294975_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294975_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5646615
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC396940.7029698323685961No Hit
CCCATGTACTCTGCGTTGATACCAC363640.6439964474291235No Hit
GAGTACATGGGAAGCAGTGGTATCA173100.30655534333401513No Hit
CATGTACTCTGCGTTGATACCACTG129750.2297836845614585No Hit
GCGTTGATACCACTGCTTCCCATGT118210.20934666167252416No Hit
GTATCAACGCAGAGTACATGGGAAG115080.2038035176827179No Hit
ACGCAGAGTACATGGGAAGCAGTGG102160.1809225527152108No Hit
GCTTCCCATGTACTCTGCGTTGATA96170.17031442731618854No Hit
TATCAACGCAGAGTACATGGGAAGC89760.15896249345847024No Hit
GGTATCAACGCAGAGTACATGGGAA89070.15774052241918388No Hit
ACTCTGCGTTGATACCACTGCTTCC81550.14442280906348318No Hit
GTATCAACGCAGAGTACTTTTTTTT79430.14066834731958883No Hit
GTGGTATCAACGCAGAGTACATGGG67880.12021361470544742No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN65960.11681334746569405No Hit
GGTATCAACGCAGAGTACTTTTTTT59350.10510721910383478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGCG400.005284032314.2489219
TGCGAAA909.553105E-510.55241612
TATTCCG1856.002665E-1110.2752385
ACTGTCG856.6032645E-410.0580628
GTCTAGG6350.010.0343531
TACACCG951.6375516E-410.0048375
GTAGAAC4300.09.5038373
CGCTACA800.0045045239.5035832
GCGCTCC1002.759818E-49.4992819
CGAGCTT800.0045286669.49742714
GTCCTAG6700.09.3682281
GTGTTAG9400.09.1054821
GACCGGG1054.4995084E-49.0469347
AGACCGG950.00182773828.998526
CGAATTG950.00182891938.99788219
GTCTTGC10250.08.9070861
GTCTTAG6200.08.7432211
CCAGGAC9150.08.72483353
AGGACCG1107.1153487E-48.6405415
TGGACAG8750.08.5812935
Produced by FastQC (version 0.11.5)