FastQCFastQC Report
Wed 25 May 2016
SRR1294975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294975_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5646615
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC405580.7182710349474862No Hit
GTACATGGGAAGCAGTGGTATCAAC381840.6762281473059523No Hit
GTATCAACGCAGAGTACTTTTTTTT221650.3925360592142372No Hit
GAGTACATGGGAAGCAGTGGTATCA183480.32493803809893185No Hit
GGTATCAACGCAGAGTACTTTTTTT180290.31928863575788324No Hit
TATCAACGCAGAGTACTTTTTTTTT146590.2596068618101287No Hit
CATGTACTCTGCGTTGATACCACTG145710.2580484059919084No Hit
GCGTTGATACCACTGCTTCCCATGT128540.22764080781140558No Hit
ACGCAGAGTACATGGGAAGCAGTGG122690.21728061856528202No Hit
GTATCAACGCAGAGTACATGGGAAG113830.20158980203183677No Hit
GCTTCCCATGTACTCTGCGTTGATA105500.1868376009343651No Hit
TATCAACGCAGAGTACATGGGAAGC98920.17518460174812697No Hit
ACTCTGCGTTGATACCACTGCTTCC95650.169393521605422No Hit
GGTATCAACGCAGAGTACATGGGAA95070.16836635754341317No Hit
ACGCAGAGTACTTTTTTTTTTTTTT87350.1546944496835715No Hit
GTGGTATCAACGCAGAGTACATGGG70440.12474730435845192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAG500.001500932713.2993758
AACCGTG1153.783498E-1013.2167697
TATTCCG2150.011.0458945
GTCGTAG803.7298474E-410.7011171
AACGCCG750.002650647710.1327676
ACAGCGC1151.0282496E-59.9125778
GTATTAG8600.09.8439211
ACCGCTC1002.7590283E-49.4995538
TATACCG900.00111503999.4994695
GTCTAGG5750.09.429391
GTACTAG3800.09.2617851
ACACCGG1151.11234505E-49.0864486
TAGACAG9800.09.0148025
CTAGGAC5800.09.0081173
GTGCGCC950.00182637768.99925811
GAACCGT850.00744323348.9406776
GCGCGTG850.00744485538.9404389
CTGTGCG1606.758819E-78.9055169
TGTGCGA1401.0875048E-58.82070110
GCGTCAG1304.30831E-58.7804031
Produced by FastQC (version 0.11.5)