Basic Statistics
Measure | Value |
---|---|
Filename | SRR1294974_2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 4439332 |
Sequences flagged as poor quality | 0 |
Sequence length | 25 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTACATGGGAAGCAGTGGTATCAAC | 15833 | 0.35665275766714455 | No Hit |
CCCATGTACTCTGCGTTGATACCAC | 14162 | 0.31901195945696337 | No Hit |
GAGTACATGGGAAGCAGTGGTATCA | 7004 | 0.15777148453866482 | No Hit |
GTATCAACGCAGAGTACTTTTTTTT | 6694 | 0.15078845195628532 | No Hit |
ATTCCATTCCATTCCATTCCATTCC | 5581 | 0.12571711239438726 | No Hit |
NNNNNNNNNNNNNNNNNNNNNNNNN | 5316 | 0.1197477458320306 | No Hit |
CATGTACTCTGCGTTGATACCACTG | 5147 | 0.11594086677905593 | No Hit |
GGTATCAACGCAGAGTACTTTTTTT | 5138 | 0.11573813357505139 | No Hit |
GCGTTGATACCACTGCTTCCCATGT | 4720 | 0.10632230254461707 | No Hit |
GTATCAACGCAGAGTACATGGGAAG | 4580 | 0.10316867492676826 | No Hit |
GCTTCCCATGTACTCTGCGTTGATA | 4496 | 0.10127649835605897 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCGTCCA | 65 | 5.463569E-5 | 13.152143 | 9 |
ACCGTCC | 85 | 6.6059385E-4 | 10.057521 | 8 |
CCGCCCG | 85 | 6.612112E-4 | 10.056499 | 17 |
CTAGGAC | 395 | 0.0 | 9.865052 | 3 |
GTATTAG | 755 | 0.0 | 9.699204 | 1 |
TAGGACT | 550 | 0.0 | 9.331755 | 4 |
GTCTCGC | 260 | 0.0 | 9.14448 | 1 |
CTAATAC | 585 | 0.0 | 9.097975 | 3 |
GTACTAC | 335 | 0.0 | 9.084427 | 1 |
AAGACCG | 105 | 4.477124E-4 | 9.0513525 | 5 |
AGACGGT | 105 | 4.505006E-4 | 9.045732 | 6 |
AGGAGCG | 95 | 0.0018180078 | 9.003714 | 5 |
GTATTAC | 520 | 0.0 | 8.96159 | 1 |
GTCCTAG | 490 | 0.0 | 8.927998 | 1 |
TCTGTCG | 385 | 0.0 | 8.881967 | 8 |
TCGAGTG | 150 | 2.7131737E-6 | 8.864617 | 19 |
TCAGGAC | 560 | 0.0 | 8.825268 | 3 |
CCTAGAC | 540 | 0.0 | 8.800125 | 3 |
GTCTAAG | 455 | 0.0 | 8.778701 | 1 |
CTTACAC | 700 | 0.0 | 8.689495 | 3 |