FastQCFastQC Report
Wed 25 May 2016
SRR1294974_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294974_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4439332
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC158330.35665275766714455No Hit
CCCATGTACTCTGCGTTGATACCAC141620.31901195945696337No Hit
GAGTACATGGGAAGCAGTGGTATCA70040.15777148453866482No Hit
GTATCAACGCAGAGTACTTTTTTTT66940.15078845195628532No Hit
ATTCCATTCCATTCCATTCCATTCC55810.12571711239438726No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN53160.1197477458320306No Hit
CATGTACTCTGCGTTGATACCACTG51470.11594086677905593No Hit
GGTATCAACGCAGAGTACTTTTTTT51380.11573813357505139No Hit
GCGTTGATACCACTGCTTCCCATGT47200.10632230254461707No Hit
GTATCAACGCAGAGTACATGGGAAG45800.10316867492676826No Hit
GCTTCCCATGTACTCTGCGTTGATA44960.10127649835605897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCA655.463569E-513.1521439
ACCGTCC856.6059385E-410.0575218
CCGCCCG856.612112E-410.05649917
CTAGGAC3950.09.8650523
GTATTAG7550.09.6992041
TAGGACT5500.09.3317554
GTCTCGC2600.09.144481
CTAATAC5850.09.0979753
GTACTAC3350.09.0844271
AAGACCG1054.477124E-49.05135255
AGACGGT1054.505006E-49.0457326
AGGAGCG950.00181800789.0037145
GTATTAC5200.08.961591
GTCCTAG4900.08.9279981
TCTGTCG3850.08.8819678
TCGAGTG1502.7131737E-68.86461719
TCAGGAC5600.08.8252683
CCTAGAC5400.08.8001253
GTCTAAG4550.08.7787011
CTTACAC7000.08.6894953