##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294974_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4439332
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.034824608747442	33.0	33.0	33.0	27.0	33.0
2	31.10355048912764	33.0	33.0	33.0	27.0	33.0
3	31.125493430092636	33.0	33.0	33.0	27.0	33.0
4	34.7299837002504	37.0	37.0	37.0	27.0	37.0
5	34.8933479181102	37.0	37.0	37.0	33.0	37.0
6	34.88176892379304	37.0	37.0	37.0	33.0	37.0
7	34.76455984819338	37.0	37.0	37.0	27.0	37.0
8	34.68151199324583	37.0	37.0	37.0	27.0	37.0
9	34.67714827365919	37.0	37.0	37.0	27.0	37.0
10	34.58101714402077	37.0	37.0	37.0	27.0	37.0
11	34.73583412999974	37.0	37.0	37.0	27.0	37.0
12	34.64902534885879	37.0	37.0	37.0	27.0	37.0
13	34.57679511241781	37.0	37.0	37.0	27.0	37.0
14	35.92468866937639	40.0	37.0	40.0	27.0	40.0
15	36.076683383896494	40.0	37.0	40.0	27.0	40.0
16	36.159918429169075	40.0	37.0	40.0	27.0	40.0
17	36.09023857643447	40.0	37.0	40.0	27.0	40.0
18	36.0334617910983	40.0	37.0	40.0	27.0	40.0
19	36.13591774618344	40.0	37.0	40.0	27.0	40.0
20	36.1268783231351	40.0	37.0	40.0	27.0	40.0
21	36.12943816772433	40.0	37.0	40.0	27.0	40.0
22	36.02524749219027	40.0	37.0	40.0	27.0	40.0
23	35.932965590318545	37.0	37.0	40.0	27.0	40.0
24	35.9801226851247	37.0	37.0	40.0	27.0	40.0
25	34.76791981316108	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4562442390987229
1101	2	0.4500122448627195
1101	3	0.4990495588985482
1101	4	0.5521759716222903
1101	5	0.46641320355363547
1101	6	0.335501327098612
1101	7	0.37219171905670834
1101	8	0.5372702270657967
1101	9	0.5447046324958862
1101	10	0.7261694680837323
1101	11	0.6390415813106642
1101	12	0.6175537239138436
1101	13	0.5670368585277288
1101	14	1.0635121136918144
1101	15	0.9325112638371849
1101	16	0.904318697287529
1101	17	0.8027081005212651
1101	18	0.970145535361894
1101	19	0.7842659815525508
1101	20	0.7437858336500156
1101	21	0.8054246489960804
1101	22	0.8807363868761939
1101	23	0.9686363678618264
1101	24	0.9310019728011767
1101	25	0.9584082004301067
1102	1	0.40421663278782916
1102	2	0.4296068322803457
1102	3	0.5046252430020353
1102	4	0.5750500562256278
1102	5	0.4544206946680376
1102	6	0.3204016625911166
1102	7	0.4231581798537505
1102	8	0.4985723013531569
1102	9	0.5598526287581223
1102	10	0.654356559752685
1102	11	0.46944204998720096
1102	12	0.5816455267703233
1102	13	0.5544044716587919
1102	14	0.9238564523249622
1102	15	0.816574785337643
1102	16	0.7881836218237623
1102	17	0.7887470517734485
1102	18	0.8818428657962798
1102	19	0.7501008444345203
1102	20	0.8285709890549597
1102	21	0.7884920893262546
1102	22	0.9133254617670374
1102	23	0.8768784187030576
1102	24	0.8836215149475137
1102	25	0.9016215669798129
1103	1	0.2535780807579151
1103	2	0.26251945143519606
1103	3	0.2912685268809092
1103	4	0.3037010099796902
1103	5	0.10694612730921449
1103	6	0.0779881182564992
1103	7	0.12412563291330514
1103	8	0.27049180703818365
1103	9	0.34187459173393364
1103	10	0.4237476950987684
1103	11	0.30124134121988533
1103	12	0.36506957837329423
1103	13	0.31384175903752976
1103	14	0.6885135215815836
1103	15	0.6234794179782241
1103	16	0.6080301783349071
1103	17	0.5418232894033537
1103	18	0.6232290165405345
1103	19	0.5321029969396704
1103	20	0.5488121824590664
1103	21	0.5118271239650127
1103	22	0.5913931603655698
1103	23	0.7056316191205241
1103	24	0.6617415747755473
1103	25	0.6610242014316299
1104	1	0.2467230465060446
1104	2	0.2253133980311155
1104	3	0.2660490346568629
1104	4	0.23904714698522156
1104	5	0.16200332657895444
1104	6	0.13149765577365002
1104	7	0.1562573848191562
1104	8	0.35027754521843946
1104	9	0.30437566675450967
1104	10	0.45486578026393687
1104	11	0.34799566198509524
1104	12	0.40428399911623814
1104	13	0.3189378470744373
1104	14	0.6008455118068241
1104	15	0.5297891674583752
1104	16	0.46783486467251123
1104	17	0.5658780397516949
1104	18	0.5949488630717426
1104	19	0.5274726830271206
1104	20	0.4974362950970246
1104	21	0.4721907065516078
1104	22	0.6173963957278801
1104	23	0.5881326467257395
1104	24	0.5314919999604868
1104	25	0.6417770412027153
1105	1	0.12912496993772749
1105	2	0.09586312743921965
1105	3	0.06899125745403722
1105	4	0.1447139673266591
1105	5	0.07009238083230684
1105	6	0.09357551904975026
1105	7	0.044183597169528355
1105	8	0.1814405937078334
1105	9	0.10658262673647556
1105	10	0.30859959037406526
1105	11	0.2378775150189938
1105	12	0.27298732123031755
1105	13	0.19574857201123308
1105	14	0.4926352777932692
1105	15	0.3744206351228172
1105	16	0.33003847010039067
1105	17	0.20046632227609962
1105	18	0.290721827906836
1105	19	0.3193053173437619
1105	20	0.3901001757072393
1105	21	0.36998682391513427
1105	22	0.29049303691665784
1105	23	0.4001721380611585
1105	24	0.45446868242694194
1105	25	0.22499716727323005
1106	1	-0.13175392320868795
1106	2	-0.2379592136151949
1106	3	-0.27288575378096525
1106	4	-0.22844523523292537
1106	5	-0.4956835973772087
1106	6	-0.5260088015240783
1106	7	-0.859994762986652
1106	8	-0.4535328748185634
1106	9	-0.04620726945053377
1106	10	-0.44151948462771173
1106	11	-0.5068095705513471
1106	12	-0.9245675976280054
1106	13	-0.2574185040139696
1106	14	-0.4582415385192249
1106	15	-0.26060020818660234
1106	16	-0.17325304049800394
1106	17	-0.35209336755784904
1106	18	-0.5740624062758073
1106	19	-0.8040043838952187
1106	20	-0.25460681684263875
1106	21	0.005836167638804568
1106	22	0.08568004608120106
1106	23	-0.6502075141983639
1106	24	-0.12393163863690404
1106	25	0.04266757851505787
1107	1	-1.324183741423564
1107	2	-1.2502304174151497
1107	3	-1.0265818970589358
1107	4	-1.1303542054022273
1107	5	-1.1882661974208943
1107	6	-1.1112329678214223
1107	7	-1.2322266520717378
1107	8	-1.1634437211198616
1107	9	-1.2023704334088876
1107	10	-0.9993138336120708
1107	11	-1.0122299884448225
1107	12	-1.1006650022669788
1107	13	-1.0407939154832135
1107	14	-1.3631005102488203
1107	15	-1.1338986614464588
1107	16	-1.1878478989836125
1107	17	-1.192992512971692
1107	18	-1.405990421863521
1107	19	-1.3250667659605497
1107	20	-1.4509985456637295
1107	21	-1.3775730109595514
1107	22	-1.2890315263576753
1107	23	-1.089923808984679
1107	24	-1.0517644338066177
1107	25	-0.9297377594331167
1108	1	0.06827486342837474
1108	2	-0.1257541410934735
1108	3	-0.02573239859284726
1108	4	-0.16577071458928572
1108	5	-0.43142260925347387
1108	6	-0.029932488180143935
1108	7	0.016694582929666524
1108	8	0.19455870299636047
1108	9	-0.20305603423457796
1108	10	-0.5380169124155785
1108	11	-0.12530652424534594
1108	12	0.1522661982814526
1108	13	0.1001449220534596
1108	14	-0.22169862325095124
1108	15	0.05277908703020984
1108	16	-0.8343085656892626
1108	17	-0.810973498468357
1108	18	-0.6391468194337975
1108	19	-0.1374748038146052
1108	20	-1.0512301535910993
1108	21	-0.1803736638878135
1108	22	-0.2990509730088178
1108	23	-0.10598958102457345
1108	24	-0.4097273988825165
1108	25	-1.064917894706312
1109	1	0.021053801999208588
1109	2	0.02579900001252966
1109	3	0.11704514702632096
1109	4	0.11964508148258801
1109	5	0.043434613200908245
1109	6	0.03971509504871307
1109	7	0.07909881750272518
1109	8	0.21113482780483395
1109	9	0.19100999167432064
1109	10	0.1609662328692778
1109	11	0.1130275055638208
1109	12	0.12468575532282955
1109	13	0.13078985867416293
1109	14	0.4668373845495779
1109	15	0.3510100384161987
1109	16	0.21319108607282544
1109	17	0.28709029871787806
1109	18	0.23228374192572687
1109	19	0.28152505689023144
1109	20	0.29923648521653234
1109	21	0.2136923620210851
1109	22	0.2485183263364945
1109	23	0.3818220919103723
1109	24	0.4707573482967575
1109	25	0.35477844183080975
1110	1	-0.08640951209557812
1110	2	-0.022776526687145804
1110	3	0.055394652784279685
1110	4	-1.1016393013433188
1110	5	-0.46343352218097067
1110	6	-0.11322006805104223
1110	7	-0.038399387822067865
1110	8	0.0550473267205831
1110	9	0.03564547668776186
1110	10	-0.11346431383983457
1110	11	-0.10770196392158482
1110	12	-2.2765029051186048E-4
1110	13	0.060496203999200304
1110	14	0.03551298285022142
1110	15	-0.019293545980978877
1110	16	0.0599774489132443
1110	17	0.10605111491778985
1110	18	0.09743323075432642
1110	19	0.0774765324074167
1110	20	0.1387250835932221
1110	21	0.12201841084508658
1110	22	-0.4870751466501275
1110	23	-0.07891760030544503
1110	24	0.05408818175857277
1110	25	0.08139807370554308
1111	1	-0.157250964928636
1111	2	-0.30387101506744685
1111	3	-0.19325881816680734
1111	4	-0.15345282974567453
1111	5	-0.16811286298404582
1111	6	-0.05992148344595449
1111	7	-0.028197271540307156
1111	8	0.05708103039096102
1111	9	0.034646897931644105
1111	10	-0.1297968651209942
1111	11	-0.1862138360211958
1111	12	-0.007571726413530655
1111	13	-0.16019013641217583
1111	14	-0.2334541764198761
1111	15	-0.17686112946352495
1111	16	-0.27532257031158025
1111	17	-0.17423449043894834
1111	18	-0.09428257239014215
1111	19	-0.1749025492760765
1111	20	-0.10315796917563347
1111	21	-0.19868853154779487
1111	22	-0.1592158223302178
1111	23	0.041755756029608904
1111	24	-0.009533752647975291
1111	25	0.037810675913100056
1112	1	-0.10209849565054796
1112	2	-0.39571705975950877
1112	3	-0.17043119681179064
1112	4	-0.13111035463136744
1112	5	-0.09742384129520332
1112	6	-0.07121639334415164
1112	7	-0.09282990419993808
1112	8	0.013378981010298219
1112	9	-0.10039375536565842
1112	10	0.17314507345316343
1112	11	-0.06872296635859954
1112	12	0.01022511581391683
1112	13	-0.05863697774047694
1112	14	-0.2714161902927543
1112	15	-0.17541821736815422
1112	16	-0.1666521828485017
1112	17	-0.31096503268453546
1112	18	-0.23882259792566174
1112	19	-0.1252202515044658
1112	20	-0.9703906840655208
1112	21	-1.4192328124581195
1112	22	-1.8242522905489622
1112	23	-0.6879971485947394
1112	24	-0.36232159123473195
1112	25	-1.4909475981997389
1113	1	-0.2403502238789592
1113	2	-0.2184908152305276
1113	3	-0.25652325309681245
1113	4	-0.6074851654465974
1113	5	-0.36550653037770786
1113	6	-0.2737386864680573
1113	7	-0.20052358849657992
1113	8	-0.08393244779487219
1113	9	-0.19222352998013292
1113	10	-0.043724276569328424
1113	11	-0.08226031245423115
1113	12	-0.13229891576460062
1113	13	-0.14703217960573767
1113	14	-0.6234843377137054
1113	15	-0.4437184178017475
1113	16	-0.48852276001543515
1113	17	-0.5219300795205513
1113	18	-0.9156950158092485
1113	19	-1.105047939973396
1113	20	-1.2556007302879593
1113	21	-1.0989740895064273
1113	22	-0.5220365790097148
1113	23	-2.1373440009692644
1113	24	-1.113050208242953
1113	25	-0.6708293832858416
1114	1	-0.18613377632165395
1114	2	-0.41982853194951986
1114	3	-0.32406487120709215
1114	4	-0.5429479504098964
1114	5	-0.513579557815639
1114	6	-0.33082918598809385
1114	7	-0.3693822047923163
1114	8	-0.09092512235005756
1114	9	-1.1265597485344685
1114	10	-0.37106993659124043
1114	11	-0.3662326624053662
1114	12	-0.33713509605590275
1114	13	-0.348039899493628
1114	14	-0.6731066533308265
1114	15	-1.2189208292459384
1114	16	-1.0158959077893215
1114	17	-1.5139004921809374
1114	18	-1.7658341751216184
1114	19	-1.5510915064167676
1114	20	-0.837820510067516
1114	21	-0.6408306529519265
1114	22	-0.48694981950853844
1114	23	-0.23259674499367833
1114	24	-0.8647008343956557
1114	25	-0.5071942410783308
1115	1	-0.7783494589157556
1115	2	-0.611438658600342
1115	3	-0.7441613313357252
1115	4	-0.7737257627738785
1115	5	-0.4962907552070561
1115	6	-0.44122119044760666
1115	7	-0.47916804830612136
1115	8	-0.2757663015959082
1115	9	-0.65903740533345
1115	10	-0.3349745047092938
1115	11	-0.5620340399358312
1115	12	-0.6235814776189699
1115	13	-0.5661132186225615
1115	14	-0.5343910095577158
1115	15	-1.6433500292977783
1115	16	-1.0212797858004095
1115	17	-0.7646091012775642
1115	18	-2.5015075405740035
1115	19	-1.3360069636560468
1115	20	-1.090888027931122
1115	21	-1.0899683882557696
1115	22	-0.8500638468267141
1115	23	-0.4725755716249367
1115	24	-0.6572653459442606
1115	25	-0.6554580031726687
1116	1	-1.4271892137130209
1116	2	-0.8598012525981318
1116	3	-1.744877870602771
1116	4	-0.9962695362707876
1116	5	-0.988097983993768
1116	6	-0.5720589199209059
1116	7	-1.2998179161025831
1116	8	-2.9794358291069436
1116	9	-1.6535404397711488
1116	10	-0.7975675648277871
1116	11	-0.6031390469784839
1116	12	-0.7974152160783632
1116	13	-1.9115585536171196
1116	14	-1.7438504028311854
1116	15	-1.4917699120013523
1116	16	-1.0690700418482564
1116	17	-1.7665152734899436
1116	18	-2.499071804985924
1116	19	-1.4092172389631514
1116	20	-1.0233978281691591
1116	21	-0.7034853352028492
1116	22	-0.5391332236541686
1116	23	-0.4592318542580287
1116	24	-0.5285088284581931
1116	25	-0.5782507575450921
1201	1	0.48016794293172893
1201	2	0.3837568455854701
1201	3	0.46008046750400666
1201	4	0.4997463123354109
1201	5	0.38605016908864087
1201	6	0.24043579296478157
1201	7	0.3110311493898905
1201	8	0.4031815829730263
1201	9	0.40724694669410155
1201	10	0.6479424985004343
1201	11	0.49811238772240785
1201	12	0.5528536383672602
1201	13	0.5809748036186235
1201	14	0.89842915248407
1201	15	0.8371576857159866
1201	16	0.7155827440744602
1201	17	0.7699457793159041
1201	18	0.671942385118065
1201	19	0.592983108738828
1201	20	0.5899151290853482
1201	21	0.6732936723412593
1201	22	0.6632924760146679
1201	23	0.876682657933678
1201	24	0.7685867198403713
1201	25	0.9645124220077363
1202	1	0.4217417714425018
1202	2	0.47292716143698144
1202	3	0.4969606486040874
1202	4	0.5372284090576542
1202	5	0.4109563791370121
1202	6	0.20972972043185223
1202	7	0.2886715640073376
1202	8	0.3752932065309622
1202	9	0.4159703449551344
1202	10	0.5869856925137142
1202	11	0.4377852897932897
1202	12	0.39915347534368806
1202	13	0.42455745413369783
1202	14	0.8586182019599349
1202	15	0.6580194491878899
1202	16	0.591098141464407
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2210	20	0.390641185638664
2210	21	0.48531955857011866
2210	22	0.6189769109717105
2210	23	0.6198485871872208
2210	24	0.555782932536097
2210	25	0.6037228860757295
2211	1	0.21548792262708716
2211	2	0.003474570200022953
2211	3	0.07831285403024069
2211	4	0.1634692246552376
2211	5	0.20686331258860235
2211	6	0.18420786881391393
2211	7	0.22658912708934054
2211	8	0.26237994116821994
2211	9	0.16396883717454358
2211	10	0.2494864490236921
2211	11	0.05657408503790151
2211	12	0.2203229120848249
2211	13	0.055339722373176414
2211	14	0.07329716882907178
2211	15	0.08346690189142691
2211	16	0.0819601816440283
2211	17	0.11838911274239905
2211	18	0.24786982471292873
2211	19	0.13090006416879163
2211	20	0.20826267209697136
2211	21	0.1714069486487375
2211	22	0.14580141244110933
2211	23	0.3276679579169368
2211	24	0.21133006168403767
2211	25	0.25121813107312363
2212	1	0.1509732608493799
2212	2	0.18710738599346044
2212	3	0.11902811611816944
2212	4	0.15209743235394058
2212	5	0.2641322272969049
2212	6	0.16769956360865024
2212	7	0.24039194541253295
2212	8	0.3497966972875233
2212	9	0.11164116064156104
2212	10	0.3298378068449068
2212	11	0.14908528642879304
2212	12	0.23389856126528485
2212	13	0.14013011882369142
2212	14	0.15183537634375455
2212	15	0.19482801624140222
2212	16	0.11617609272632734
2212	17	0.20353820351493823
2212	18	0.2844636764715034
2212	19	0.20601918656492302
2212	20	0.2550860090747875
2212	21	0.19941040133690535
2212	22	-0.3472781969002412
2212	23	0.2587541451669608
2212	24	0.20618500563343645
2212	25	0.2061151677933566
2213	1	-0.09012089799263734
2213	2	-0.812955636374781
2213	3	-0.19374259305041264
2213	4	-0.09636618463749613
2213	5	-0.05147139342133045
2213	6	-0.005450853200748895
2213	7	-0.07031127028156448
2213	8	0.04000040016696005
2213	9	-0.29363199325521094
2213	10	-1.5614152397440009
2213	11	-0.44894969237834204
2213	12	-0.16895666538688658
2213	13	-0.18795146655291717
2213	14	-0.04023428577336574
2213	15	-0.022289168474742382
2213	16	-0.156081137731519
2213	17	-0.22597428558211874
2213	18	-0.04812418232922511
2213	19	-0.041893864851061835
2213	20	-0.08729902312435911
2213	21	-0.04913945068143022
2213	22	0.11901345379079942
2213	23	-0.8737691669676053
2213	24	-1.4753353252413532
2213	25	-1.2871004587904835
2214	1	-0.015649409233688516
2214	2	-0.19976153285006504
2214	3	-0.03420537917684996
2214	4	0.013400089806566484
2214	5	0.021172653787758122
2214	6	-0.026856218928386966
2214	7	-0.005508616882679007
2214	8	-0.008853780051815363
2214	9	-0.3019266072489799
2214	10	0.02472834086788822
2214	11	-0.12191379341745545
2214	12	-0.11853029195373921
2214	13	-0.04373290152879861
2214	14	-0.01862319426740555
2214	15	-0.06407801873340446
2214	16	-0.280026825178993
2214	17	-0.16136261957502995
2214	18	0.01562715256018521
2214	19	-0.7487623515249737
2214	20	-0.3650473701659749
2214	21	-0.22663889064247655
2214	22	-0.5470358382398572
2214	23	-1.7464192279119146
2214	24	-1.257898330265384
2214	25	-1.6197677038648308
2215	1	-0.4419121588274777
2215	2	-0.2639381111196535
2215	3	-1.2941118142565458
2215	4	-0.9568148305672111
2215	5	-0.3653438663011599
2215	6	-0.3075849227261571
2215	7	-0.2044370674601126
2215	8	-0.17112552724395158
2215	9	-0.434930582024748
2215	10	-1.4974305695688344
2215	11	-0.657813015554396
2215	12	-0.6445997119028704
2215	13	-0.5555981907579408
2215	14	-0.9537613639144098
2215	15	-1.207075155868516
2215	16	-0.8015451240518559
2215	17	-0.6588937575439147
2215	18	-0.6082311058273504
2215	19	-1.0869307357255735
2215	20	-1.4518038471233012
2215	21	-0.78375985382241
2215	22	-0.44855185441372214
2215	23	-0.7363864923352494
2215	24	-1.896302802489366
2215	25	-0.9378789819824291
2216	1	-0.45079411488706356
2216	2	-0.5332362570212723
2216	3	-1.3708500070292082
2216	4	-0.6867784537753252
2216	5	-0.49196277988462356
2216	6	-0.48967925223825404
2216	7	-0.3880258540081414
2216	8	-0.40679424199473146
2216	9	-0.3042408694060015
2216	10	-0.31802642438090345
2216	11	-0.43521289769610405
2216	12	-0.37480807909252434
2216	13	-1.1555391595135802
2216	14	-0.7703142846847442
2216	15	-1.2331503234541188
2216	16	-0.8838112635837518
2216	17	-1.8023385752421959
2216	18	-0.888170063198416
2216	19	-0.6073555207482144
2216	20	-0.5704585201295131
2216	21	-0.3689912540881082
2216	22	-0.4192920796717772
2216	23	-0.325290541293235
2216	24	-0.42400600891205187
2216	25	-0.3029304773413557
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5912.0
3	612.0
4	819.0
5	689.0
6	451.0
7	324.0
8	495.0
9	1008.0
10	1971.0
11	3992.0
12	6944.0
13	10452.0
14	14290.0
15	17832.0
16	20216.0
17	21373.0
18	22301.0
19	23487.0
20	24825.0
21	27087.0
22	29513.0
23	31742.0
24	33622.0
25	35926.0
26	38476.0
27	43053.0
28	49191.0
29	58028.0
30	69601.0
31	88459.0
32	117656.0
33	164062.0
34	234869.0
35	368848.0
36	660142.0
37	2211064.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.25037629953556	21.135255193340342	13.930799752606077	22.683568754518024
2	14.984057448699827	20.83939354891415	41.081999258444	23.09454974394202
3	18.139464335572473	26.25446825280566	29.70964623468233	25.896421176939537
4	11.802701115678214	17.8725468748977	38.90145237513102	31.423299634293056
5	12.953253443450274	38.52152379421091	35.134077432636104	13.391145329702713
6	30.999557826637798	38.61053735913483	17.498079739724886	12.891825074502487
7	26.01495403377026	32.90587102010453	23.311622418772533	17.767552527352684
8	23.658411373404416	37.53483978777134	21.325765391702383	17.480983447121858
9	23.468098190533123	17.612231555411874	22.421613548155225	36.49805670589978
10	14.249121771597622	30.742069415209052	33.92351516237997	21.085293650813362
11	31.697111777436486	26.284752976569365	23.162187930843007	18.855947315151138
12	20.506316484258676	28.0623955416664	31.97770381733979	19.45358415673513
13	26.136682011690393	24.390234541587454	26.86559885890999	22.607484587812163
14	19.853162266440687	23.894540854425493	28.617556418575123	27.634740460558692
15	22.818266892442455	31.007905914516087	24.164367000921374	22.009460192120088
16	21.135088148171725	32.03535903820382	26.140809094645828	20.68874371897862
17	19.571296716601992	30.84977936647061	28.9975366783915	20.581387238535896
18	19.728669842967463	30.51878501189928	29.7101804225231	20.042364722610163
19	20.378749060407078	29.344892113288385	30.017448776666946	20.258910049637592
20	21.873802278227636	28.957408167328246	29.038328451030022	20.130461103414092
21	21.972177950785614	29.492812227237852	28.234949172428227	20.30006064954831
22	20.52458758848031	29.193067707878363	29.55950524386696	20.722839459774374
23	20.44531927188674	29.640187084657615	29.126155735336607	20.78833790811904
24	20.6431496392931	28.938121506547905	29.33192575538035	21.08680309877865
25	21.277556390536123	28.812112214886792	28.201724562967133	21.708606831609945
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8523.0
1	8523.0
2	6832.5
3	5142.0
4	5142.0
5	5142.0
6	7001.0
7	8860.0
8	8860.0
9	8860.0
10	13336.0
11	17812.0
12	17812.0
13	17812.0
14	29978.5
15	42145.0
16	42145.0
17	42145.0
18	67774.0
19	93403.0
20	93403.0
21	93403.0
22	134464.5
23	175526.0
24	175526.0
25	175526.0
26	227797.5
27	280069.0
28	280069.0
29	280069.0
30	334190.5
31	388312.0
32	388312.0
33	388312.0
34	433026.0
35	477740.0
36	477740.0
37	477740.0
38	506290.0
39	534840.0
40	534840.0
41	534840.0
42	528047.0
43	521254.0
44	521254.0
45	521254.0
46	529271.5
47	537289.0
48	537289.0
49	537289.0
50	497405.5
51	457522.0
52	457522.0
53	457522.0
54	404750.0
55	351978.0
56	351978.0
57	351978.0
58	301418.5
59	250859.0
60	250859.0
61	250859.0
62	204096.5
63	157334.0
64	157334.0
65	157334.0
66	118833.5
67	80333.0
68	80333.0
69	80333.0
70	56760.0
71	33187.0
72	33187.0
73	33187.0
74	22635.5
75	12084.0
76	12084.0
77	12084.0
78	7919.0
79	3754.0
80	3754.0
81	3754.0
82	2395.5
83	1037.0
84	1037.0
85	1037.0
86	627.5
87	218.0
88	218.0
89	218.0
90	143.0
91	68.0
92	68.0
93	68.0
94	42.5
95	17.0
96	17.0
97	17.0
98	21.5
99	26.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3153852876964372
2	0.18266261680811435
3	0.20068334605296473
4	0.22140718468454265
5	0.24936184092561675
6	0.20144922704587084
7	0.1957051195990748
8	0.22007815590273494
9	0.21940237855605305
10	0.23077796389186483
11	0.2041748623441545
12	0.21030191028740358
13	0.22262358390857
14	0.21192377591944012
15	0.20223763395033306
16	0.20545883930285008
17	0.20577420206463495
18	0.207936689574017
19	0.20773395637001243
20	0.20376939593614532
21	0.20185469345388002
22	0.2051885283641773
23	0.20840973371669433
24	0.22467344186017177
25	0.21823103115513773
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4439332.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.86722525520503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.08174464009878	74.56609705088029
2	7.1461258306437845	11.700669861587077
3	0.9978200581883127	2.4506627840359307
4	0.2780016190008877	0.9103688465622943
5	0.11482830468645087	0.47003373427194933
6	0.07638054992913117	0.3751838211698769
7	0.04745022957403809	0.2719233043064277
8	0.03719107411192317	0.24357840334430705
9	0.025194011729245333	0.1856307449988365
>10	0.1670889809083964	2.575256138464505
>50	0.014579228270045824	0.8198005883426313
>100	0.011573237569031647	1.7985006057538075
>500	8.310559013503969E-4	0.4731012703827788
>1k	9.695646186691956E-4	1.6879100511248246
>5k	1.6621107748600618E-4	0.7910342024159122
>10k+	5.540369249533539E-5	0.6802485923585231
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	15833	0.35665275766714455	No Hit
CCCATGTACTCTGCGTTGATACCAC	14162	0.31901195945696337	No Hit
GAGTACATGGGAAGCAGTGGTATCA	7004	0.15777148453866482	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6694	0.15078845195628532	No Hit
ATTCCATTCCATTCCATTCCATTCC	5581	0.12571711239438726	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5316	0.1197477458320306	No Hit
CATGTACTCTGCGTTGATACCACTG	5147	0.11594086677905593	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5138	0.11573813357505139	No Hit
GCGTTGATACCACTGCTTCCCATGT	4720	0.10632230254461707	No Hit
GTATCAACGCAGAGTACATGGGAAG	4580	0.10316867492676826	No Hit
GCTTCCCATGTACTCTGCGTTGATA	4496	0.10127649835605897	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.2525911556062938E-5	0.0
5	0.0	0.0	0.0	2.2525911556062938E-5	0.0
6	0.0	0.0	0.0	2.2525911556062938E-5	0.0
7	0.0	0.0	0.0	4.5051823112125875E-5	0.0
8	0.0	0.0	0.0	4.5051823112125875E-5	0.0
9	0.0	0.0	0.0	9.010364622425175E-5	0.0
10	0.0	0.0	0.0	1.126295577803147E-4	0.0
11	0.0	0.0	0.0	1.126295577803147E-4	0.0
12	0.0	0.0	0.0	1.126295577803147E-4	0.0
13	0.0	0.0	0.0	1.126295577803147E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCCA	65	5.463569E-5	13.152143	9
ACCGTCC	85	6.6059385E-4	10.057521	8
CCGCCCG	85	6.612112E-4	10.056499	17
CTAGGAC	395	0.0	9.865052	3
GTATTAG	755	0.0	9.699204	1
TAGGACT	550	0.0	9.331755	4
GTCTCGC	260	0.0	9.14448	1
CTAATAC	585	0.0	9.097975	3
GTACTAC	335	0.0	9.084427	1
AAGACCG	105	4.477124E-4	9.0513525	5
AGACGGT	105	4.505006E-4	9.045732	6
AGGAGCG	95	0.0018180078	9.003714	5
GTATTAC	520	0.0	8.96159	1
GTCCTAG	490	0.0	8.927998	1
TCTGTCG	385	0.0	8.881967	8
TCGAGTG	150	2.7131737E-6	8.864617	19
TCAGGAC	560	0.0	8.825268	3
CCTAGAC	540	0.0	8.800125	3
GTCTAAG	455	0.0	8.778701	1
CTTACAC	700	0.0	8.689495	3
>>END_MODULE