FastQCFastQC Report
Wed 25 May 2016
SRR1294974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294974_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4439332
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT168370.3792687728694317No Hit
CCCATGTACTCTGCGTTGATACCAC162660.36640647737091975No Hit
GTACATGGGAAGCAGTGGTATCAAC155840.35104380568968485No Hit
GGTATCAACGCAGAGTACTTTTTTT143400.3230215717139425No Hit
TATCAACGCAGAGTACTTTTTTTTT109540.24674883518511345No Hit
GAGTACATGGGAAGCAGTGGTATCA75540.17016073589449943No Hit
ACGCAGAGTACTTTTTTTTTTTTTT62870.1416204059529677No Hit
CATGTACTCTGCGTTGATACCACTG58560.13191173807230455No Hit
ATTCCATTCCATTCCATTCCATTCC54540.12285632162676727No Hit
GCGTTGATACCACTGCTTCCCATGT50420.11357564606566935No Hit
ACGCAGAGTACATGGGAAGCAGTGG49890.112381772753198No Hit
GCTTCCCATGTACTCTGCGTTGATA47590.10720081309530352No Hit
GTATCAACGCAGAGTACATGGGAAG47390.10675029486418228No Hit
GGTATCAACGCAGAGTACATGGGAA46910.10566905110949124No Hit
GAATGGAATGGAATGGAATGGAATG45790.1031461490152122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG400.00528388914.2488855
TGCACCG852.705401E-713.4107155
GAACCGA500.001501197213.2989596
GCACCGT1102.755769E-912.9535326
TCGTATA658.033086E-411.69152452
CGGCACT855.3320393E-511.1755964
GGACCGT700.001493322110.8562936
TACAGCG803.775984E-410.6867857
CCGTTCA750.002650700310.1326549
TCTGTCG3300.010.0750838
TTAGACA5550.09.5848354
CGTATAC900.00111516889.4992573
TAGACAG6800.09.4992575
AGACCGT1106.8482346E-59.4992576
CTGTCGC3750.09.3727059
TCGCCCA4400.09.2834712
TAGGACC3400.09.2198674
GTCCTAT4250.08.9529811
CGTTCAT850.00744338338.940577510
GTCGCCC3900.08.76864411
Produced by FastQC (version 0.11.5)