FastQCFastQC Report
Wed 25 May 2016
SRR1294973_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294973_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5904399
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC317970.5385306785669465No Hit
CCCATGTACTCTGCGTTGATACCAC303570.5141420828775292No Hit
GAGTACATGGGAAGCAGTGGTATCA142440.24124385902781978No Hit
CATGTACTCTGCGTTGATACCACTG107840.18264348327408092No Hit
GCGTTGATACCACTGCTTCCCATGT96460.1633697180695275No Hit
GTATCAACGCAGAGTACATGGGAAG94420.1599146670135267No Hit
ACGCAGAGTACATGGGAAGCAGTGG83680.1417248393951696No Hit
GCTTCCCATGTACTCTGCGTTGATA78150.13235894118944197No Hit
GGTATCAACGCAGAGTACATGGGAA76190.12903938233171572No Hit
TATCAACGCAGAGTACATGGGAAGC75150.1272779837541467No Hit
GTATCAACGCAGAGTACTTTTTTTT73620.1246866954621461No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN71590.12124858093092963No Hit
ACTCTGCGTTGATACCACTGCTTCC69280.11733624370575226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG1553.6379788E-910.425155
AGTGCGA1201.5278601E-610.2906610
CCGTAGA1252.5586542E-69.8844872
TAGCCCG1106.801897E-59.5052845
TACACCG1304.2142583E-69.5052835
GCACCGT1304.2531647E-69.49842556
GTCCTAA4750.09.4101051
CTAGGAC6300.09.3534533
ACCGTCT1651.0690019E-79.2112988
GCCGTAG850.00737461878.9507881
TAGGACT8050.08.8561554
GTCCTAT4650.08.794391
GTATACG1856.473056E-88.7391131
GTCTAGG7300.08.7285511
GACAGCG1107.156748E-48.6355197
CTTACAC8600.08.5096783
TAGGACA7150.08.5085144
TAGACAG10700.08.4392715
GTATTAG8150.08.401661
GTCCTAC3650.08.337721
Produced by FastQC (version 0.11.5)