##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294973_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5904399
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.072221067715784	33.0	33.0	33.0	27.0	33.0
2	31.12966942105369	33.0	33.0	33.0	27.0	33.0
3	31.077735600185555	33.0	33.0	33.0	27.0	33.0
4	34.603365219728545	37.0	37.0	37.0	27.0	37.0
5	34.90713432476362	37.0	37.0	37.0	33.0	37.0
6	34.89278925763655	37.0	37.0	37.0	33.0	37.0
7	34.77117552523127	37.0	37.0	37.0	27.0	37.0
8	34.65998063477756	37.0	37.0	37.0	27.0	37.0
9	34.595874025451195	37.0	37.0	37.0	27.0	37.0
10	34.50130538264775	37.0	37.0	37.0	27.0	37.0
11	34.69536001886051	37.0	37.0	37.0	27.0	37.0
12	34.595326806335414	37.0	37.0	37.0	27.0	37.0
13	34.52644291146313	37.0	37.0	37.0	27.0	37.0
14	35.86703879598923	40.0	37.0	40.0	27.0	40.0
15	36.03233453565723	40.0	37.0	40.0	27.0	40.0
16	36.10875433723229	40.0	37.0	40.0	27.0	40.0
17	36.0290185334697	40.0	37.0	40.0	27.0	40.0
18	35.95879716123521	37.0	37.0	40.0	27.0	40.0
19	36.04498171617467	40.0	37.0	40.0	27.0	40.0
20	36.05651108605635	40.0	37.0	40.0	27.0	40.0
21	36.055100781637556	40.0	37.0	40.0	27.0	40.0
22	35.94473053057559	40.0	37.0	40.0	27.0	40.0
23	35.83710314970245	37.0	37.0	40.0	27.0	40.0
24	35.89093081277197	37.0	37.0	40.0	27.0	40.0
25	34.683472780210145	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.403181919385851
1101	2	0.40082559605575696
1101	3	0.4490188029451474
1101	4	0.4828345114562609
1101	5	0.34672004274371915
1101	6	0.26383138246043814
1101	7	0.2945708631967747
1101	8	0.4665341041671027
1101	9	0.4559218191314329
1101	10	0.6490737832973537
1101	11	0.49819319471581736
1101	12	0.5423255891623882
1101	13	0.5225063478376413
1101	14	1.0311068827224048
1101	15	0.8257790571570851
1101	16	0.8227814884682871
1101	17	0.7780593807020821
1101	18	0.787341657074947
1101	19	0.7067669712145062
1101	20	0.722774933596348
1101	21	0.6941032636196667
1101	22	0.674043226620789
1101	23	0.8129968411992934
1101	24	0.7653792245853026
1101	25	0.8458068094951159
1102	1	0.4073746107967757
1102	2	0.4175191659258708
1102	3	0.35874656452921627
1102	4	0.4975919016550705
1102	5	0.33274967697128943
1102	6	0.15924908582965713
1102	7	0.2882603641292718
1102	8	0.34215526243582417
1102	9	0.38384974204936384
1102	10	0.5273129892162842
1102	11	0.4383219296934513
1102	12	0.45519507259061953
1102	13	0.424316350118211
1102	14	0.8296536933005854
1102	15	0.7172434678362194
1102	16	0.6976302751522212
1102	17	0.6148162110149045
1102	18	0.7193971677810538
1102	19	0.6735318563223913
1102	20	0.7130128949678394
1102	21	0.7072449743416414
1102	22	0.6720951875887238
1102	23	0.792856297040089
1102	24	0.7388860722215895
1102	25	0.8554586349585307
1103	1	0.4262017966657261
1103	2	0.3615375231389315
1103	3	0.3645200853020292
1103	4	0.40077566806866827
1103	5	0.23000350030191186
1103	6	0.19537028021333214
1103	7	0.2395134200305833
1103	8	0.38060858409592413
1103	9	0.4425835473413713
1103	10	0.5444285007266103
1103	11	0.3757584601346977
1103	12	0.4172845445605944
1103	13	0.43772901134658326
1103	14	0.8095189668213152
1103	15	0.7339996751953208
1103	16	0.6787217209335523
1103	17	0.7060653863903141
1103	18	0.7455116722489592
1103	19	0.6698048393838931
1103	20	0.731728680635598
1103	21	0.6953679674535422
1103	22	0.7449738024129502
1103	23	0.7494278141596453
1103	24	0.7234667827839232
1103	25	0.8471359875312103
1104	1	0.37830552667091766
1104	2	0.36518796593097846
1104	3	0.33126969565211795
1104	4	0.327943739036634
1104	5	0.3333068151831995
1104	6	0.20790843308035534
1104	7	0.3341692627660606
1104	8	0.4786715277151714
1104	9	0.40980975043424905
1104	10	0.5953184014819684
1104	11	0.43210808419758706
1104	12	0.45734234995085643
1104	13	0.3909488794568716
1104	14	0.7397287613758508
1104	15	0.6523250375019387
1104	16	0.5448143659381941
1104	17	0.6636409414515256
1104	18	0.6884518527406982
1104	19	0.5864216936787656
1104	20	0.6723049611231104
1104	21	0.5765256556491138
1104	22	0.6480666574577043
1104	23	0.7104773878727286
1104	24	0.6556753388130971
1104	25	0.7932410831364081
1105	1	0.19005207290168968
1105	2	0.13661021664911388
1105	3	0.12427720109814544
1105	4	0.18262512175396495
1105	5	0.16515564023023188
1105	6	0.009844804255777717
1105	7	0.0752450785976464
1105	8	0.2251795054140544
1105	9	0.22775350753047263
1105	10	0.31704422813086097
1105	11	0.1912749902587052
1105	12	0.23244098640635258
1105	13	0.27293189986991706
1105	14	0.5712790172918005
1105	15	0.41262795232248806
1105	16	0.3737779483974961
1105	17	0.33223345907242674
1105	18	0.34879910635314815
1105	19	0.41580995032236956
1105	20	0.4114564020838145
1105	21	0.444978258081008
1105	22	0.33105211359592346
1105	23	0.4782589040192349
1105	24	0.38647289854709754
1105	25	0.3006865413874209
1106	1	0.10727003127004764
1106	2	-0.041539697768811834
1106	3	-0.07697495268688215
1106	4	-0.061245115570066844
1106	5	-0.2934016928460892
1106	6	-0.3152221831860871
1106	7	-0.5898894473134533
1106	8	-0.17756217092100712
1106	9	0.14769736373794728
1106	10	-0.2696399006135266
1106	11	-0.31357400994177453
1106	12	-0.6618600892560096
1106	13	-0.18978684884655195
1106	14	-0.24621148612761345
1106	15	-0.11078139838834034
1106	16	0.0029909389796074493
1106	17	-0.16980290222747385
1106	18	-0.3515511518570591
1106	19	-0.5267489638568392
1106	20	-0.02127832439311561
1106	21	0.12497181655070477
1106	22	0.30842176512295794
1106	23	-0.5212757930153415
1106	24	0.19482627016361675
1106	25	0.30030789815101144
1107	1	-1.1857376864767488
1107	2	-1.2425396498170365
1107	3	-1.0098155795487038
1107	4	-1.1212383991442323
1107	5	-1.1759872843148145
1107	6	-1.0562412275074777
1107	7	-1.196427580452628
1107	8	-1.0222286189318197
1107	9	-1.0810519440923514
1107	10	-1.0082866957046335
1107	11	-1.0687792465827286
1107	12	-1.0812348198251556
1107	13	-0.974777935403317
1107	14	-1.4593080743204467
1107	15	-1.1328671094187612
1107	16	-1.1934369265248534
1107	17	-1.2369731975672522
1107	18	-1.4154497381164148
1107	19	-1.4592851724114198
1107	20	-1.5043365201868042
1107	21	-1.39649555224009
1107	22	-1.282325300491884
1107	23	-1.1709826503918563
1107	24	-1.0379401836962003
1107	25	-0.9863963324161631
1108	1	-0.02803101498188809
1108	2	-0.13056894697709254
1108	3	-0.029328777077093804
1108	4	-0.11784541949593574
1108	5	-0.43215604324880985
1108	6	-0.08101791489199428
1108	7	0.0025331429594714905
1108	8	0.19883312544827447
1108	9	-0.292049587420081
1108	10	-0.5592234273451382
1108	11	-0.1335164084313547
1108	12	0.12347467949661706
1108	13	0.06937122323939349
1108	14	-0.43314729237248883
1108	15	-0.0301256593205963
1108	16	-0.8521405541412648
1108	17	-0.776270556106688
1108	18	-0.7408662995178901
1108	19	-0.1317821991093453
1108	20	-1.0841225636955514
1108	21	-0.2754148722291774
1108	22	-0.2892876603814116
1108	23	-0.2391607879727502
1108	24	-0.563848248511384
1108	25	-1.210687669199082
1109	1	0.13688261693484094
1109	2	0.1212387530858976
1109	3	0.12610579288240586
1109	4	0.1592830935602123
1109	5	0.15028729389570827
1109	6	0.09146686566035811
1109	7	0.1810195848395253
1109	8	0.27628820138399846
1109	9	0.3014571112392659
1109	10	0.2782719370207616
1109	11	0.19129801452618267
1109	12	0.2935802438332189
1109	13	0.18897312775023067
1109	14	0.5854525262224541
1109	15	0.42485669143388094
1109	16	0.3127050281002681
1109	17	0.3810195453702221
1109	18	0.4485463266659764
1109	19	0.3814122817730805
1109	20	0.34648325776080924
1109	21	0.34841363494430055
1109	22	0.3622123071383143
1109	23	0.40749869636405833
1109	24	0.4433295365543586
1109	25	0.4192142492617137
1110	1	-0.03657673546287654
1110	2	-0.04257010770987435
1110	3	0.028268029418509144
1110	4	-1.1559432265865155
1110	5	-0.4035604563309505
1110	6	-0.08328089792121318
1110	7	0.0034750027980692266
1110	8	0.12317406792490004
1110	9	0.1613636762165669
1110	10	-0.12765882110215898
1110	11	-0.015360064772124815
1110	12	0.028005528434796645
1110	13	0.0771839845917981
1110	14	0.24412540424869889
1110	15	0.04592360025976916
1110	16	0.06719653485804145
1110	17	0.1504924309092246
1110	18	0.22787793253922217
1110	19	0.1617976508999206
1110	20	0.05623038921554269
1110	21	0.0848285539331286
1110	22	-0.514587575839279
1110	23	-0.027410633177993304
1110	24	0.15983699987398836
1110	25	0.21549267802045335
1111	1	-0.07406442868419916
1111	2	-0.2492495113232458
1111	3	-0.17077135551898692
1111	4	-0.15523077215200232
1111	5	-0.14446838784565585
1111	6	-0.07939599565589361
1111	7	-0.0717129085244963
1111	8	0.019487197640984277
1111	9	-0.0704196942141806
1111	10	-0.0417807823982983
1111	11	-0.21632497201654388
1111	12	-0.055135620119436624
1111	13	-0.212019162066035
1111	14	-0.28126770951749336
1111	15	-0.2075519191439028
1111	16	-0.2939871104004439
1111	17	-0.09868571775113821
1111	18	-0.22796993681527056
1111	19	-0.1961251736067524
1111	20	-0.2335935280921575
1111	21	-0.22253900020456996
1111	22	-0.1933733494902512
1111	23	0.002863437183329154
1111	24	-0.04937037296559055
1111	25	-0.09937186548380339
1112	1	-0.1751771033889966
1112	2	-0.46828916195983794
1112	3	-0.19804519018982703
1112	4	-0.20850157682144754
1112	5	-0.06462175878014165
1112	6	-0.1077761161501769
1112	7	-0.11737175150786072
1112	8	-0.07488412539552058
1112	9	-0.1135360545687476
1112	10	0.11793127883549914
1112	11	-0.06345963451177283
1112	12	-0.020897729040292745
1112	13	-0.07177758195432205
1112	14	-0.2899548967614578
1112	15	-0.18380696117888817
1112	16	-0.18587556279448592
1112	17	-0.24919809735924758
1112	18	-0.1621150128356561
1112	19	-0.1683721783257539
1112	20	-0.9893384223990012
1112	21	-1.3619217661701981
1112	22	-1.7524969768486471
1112	23	-0.6338218798108954
1112	24	-0.31389893574087324
1112	25	-1.5690099122355008
1113	1	-0.2196702904269614
1113	2	-0.19891656187804685
1113	3	-0.18577678405137732
1113	4	-0.5164451388283666
1113	5	-0.31461560236601827
1113	6	-0.19491217573663278
1113	7	-0.20749643265656914
1113	8	-0.06047947898435524
1113	9	-0.16842177010185821
1113	10	-0.05958298951951235
1113	11	-0.13926174386985934
1113	12	-0.17843506309986168
1113	13	-0.17998570352636278
1113	14	-0.5843682357350346
1113	15	-0.44437921935758595
1113	16	-0.44344433026729746
1113	17	-0.41448753139919603
1113	18	-0.8269146099734357
1113	19	-1.0845129880258426
1113	20	-1.1708164621509596
1113	21	-1.0494722213769307
1113	22	-0.4273582204312234
1113	23	-2.021600163219503
1113	24	-1.0994095984375676
1113	25	-0.6187377791091322
1114	1	-0.2951178501002474
1114	2	-0.43146350591132787
1114	3	-0.33256907936622326
1114	4	-0.5134010822406552
1114	5	-0.6993711785679437
1114	6	-0.45902821472492406
1114	7	-0.3592746650391163
1114	8	-0.1960653500734466
1114	9	-1.1611737094656647
1114	10	-0.32918371637418176
1114	11	-0.3137498246009969
1114	12	-0.3225100209495366
1114	13	-0.3751066851392366
1114	14	-0.7970135898378459
1114	15	-1.2930865612886535
1114	16	-1.2229327748746002
1114	17	-1.612772440348401
1114	18	-1.840465294397255
1114	19	-1.6132750748861255
1114	20	-0.8958717248423085
1114	21	-0.669754134038314
1114	22	-0.5643437484187501
1114	23	-0.39975505777075426
1114	24	-0.9429405053099558
1114	25	-0.5530464608739365
1115	1	-0.6220850342626747
1115	2	-0.3960583646018243
1115	3	-0.4315821842986587
1115	4	-0.39076777252360273
1115	5	-0.25411573556498723
1115	6	-0.2559685182206408
1115	7	-0.21070700297897105
1115	8	-0.09196760608196541
1115	9	-0.3351795443186134
1115	10	-0.07885711082045788
1115	11	-0.25304532501301225
1115	12	-0.44951568011114773
1115	13	-0.320876265571691
1115	14	-0.2626318208755194
1115	15	-1.3751359062580022
1115	16	-0.6683040640160058
1115	17	-0.5165580217587689
1115	18	-2.03321555925708
1115	19	-0.9408169651759053
1115	20	-0.8056696214919583
1115	21	-0.7866423909999085
1115	22	-0.6228856679724188
1115	23	-0.21204454267117967
1115	24	-0.29798571370396587
1115	25	-0.4057277838912583
1116	1	-1.429005170422375
1116	2	-0.7785684355297668
1116	3	-1.5456623230123299
1116	4	-0.9159385014054777
1116	5	-0.8898214761957348
1116	6	-0.44490301610478156
1116	7	-1.1990257257961332
1116	8	-3.0466599158543772
1116	9	-1.4159196486372636
1116	10	-0.7025612894884858
1116	11	-0.551620324768848
1116	12	-0.6729560277624529
1116	13	-1.742456823031148
1116	14	-1.6103726400681708
1116	15	-1.4815239148915893
1116	16	-1.0359831103719372
1116	17	-1.7120823109621384
1116	18	-2.5071195644878586
1116	19	-1.3136888206095563
1116	20	-0.9087628643095869
1116	21	-0.7000529655010652
1116	22	-0.4800743236338718
1116	23	-0.5001126390068151
1116	24	-0.4033256469338369
1116	25	-0.3765844996676222
1201	1	0.3694892698711989
1201	2	0.39235173606363816
1201	3	0.3628367716322245
1201	4	0.3606356480028694
1201	5	0.2933390846906647
1201	6	0.1434939417443175
1201	7	0.23504400938728764
1201	8	0.3219572978902434
1201	9	0.30560858357744536
1201	10	0.5476097596753675
1201	11	0.4230042513217853
1201	12	0.4274789420195688
1201	13	0.39260747692239306
1201	14	0.7648161297357845
1201	15	0.6872351531732761
1201	16	0.5209852523682699
1201	17	0.590282398896143
1201	18	0.4962893644798143
1201	19	0.5527594536859652
1201	20	0.5444944448605682
1201	21	0.5632629797696964
1201	22	0.6320109317764349
1201	23	0.6405094409126164
1201	24	0.5991063998082353
1201	25	0.7628447735054635
1202	1	0.33229806494892955
1202	2	0.32429606264141597
1202	3	0.3641098635813762
1202	4	0.3963758501375594
1202	5	0.29549415510336274
1202	6	0.055625243638552035
1202	7	0.13424527155118682
1202	8	0.20314491608777985
1202	9	0.1565871129859886
1202	10	0.3884097302171341
1202	11	0.174400594039831
1202	12	0.23178962396844582
1202	13	0.2579151141938638
1202	14	0.6100356272326906
1202	15	0.3662202536684802
1202	16	0.2937685763683291
1202	17	0.3209421511487065
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2210	21	0.47035199301487296
2210	22	0.5519005342504784
2210	23	0.568726504982024
2210	24	0.5467680376980795
2210	25	0.6069273579106138
2211	1	0.0907137856034872
2211	2	-0.08349049758140126
2211	3	0.04685214543102845
2211	4	-0.002174905345931677
2211	5	0.07940183551158242
2211	6	-0.04802803958800439
2211	7	0.04883242877925653
2211	8	0.12271761376354107
2211	9	0.061658126475201414
2211	10	0.1592913577941104
2211	11	0.013409725917668425
2211	12	0.035672649249747224
2211	13	-0.06063122977780466
2211	14	-0.14322005156087414
2211	15	-0.10906318717529473
2211	16	-0.10836450085223959
2211	17	-0.024690488427637547
2211	18	0.05926979417890266
2211	19	-0.03302430121996025
2211	20	0.0011229898267330896
2211	21	0.021792072254044115
2211	22	-0.11446979880059871
2211	23	0.15937041621824477
2211	24	0.15587714892615878
2211	25	-0.06245460967839733
2212	1	0.135109953321777
2212	2	0.1486612604092663
2212	3	0.16667765021758285
2212	4	0.14663395018602898
2212	5	0.198375320302155
2212	6	0.24076266243352507
2212	7	0.21010343082373595
2212	8	0.2629901804106538
2212	9	0.09958965964441546
2212	10	0.4323325162495948
2212	11	0.17242065514894023
2212	12	0.2762796757534858
2212	13	0.2385417760212647
2212	14	0.24485138701910358
2212	15	0.21204044143064493
2212	16	0.13743932305768425
2212	17	0.19358970550009502
2212	18	0.24315661105450204
2212	19	0.2751394282956312
2212	20	0.30146168035626886
2212	21	0.30845842844390603
2212	22	-0.36918389114011063
2212	23	0.24114225487615926
2212	24	0.24773253207255408
2212	25	0.2758727942090431
2213	1	-0.1248299078741475
2213	2	-0.8460603915308447
2213	3	-0.25031799711514324
2213	4	-0.11446583484280382
2213	5	-0.10441051649723931
2213	6	-0.04286824371956044
2213	7	-0.14002774528260886
2213	8	0.11579582765403273
2213	9	-0.32529387367220153
2213	10	-1.647111158502696
2213	11	-0.493157416299141
2213	12	-0.1215491375305433
2213	13	-0.15048216089473954
2213	14	-0.009360948626728316
2213	15	-0.09786546599845991
2213	16	-0.16250132517039617
2213	17	-0.18484592548977474
2213	18	-0.00289730395311949
2213	19	-0.1287949430143911
2213	20	-0.0971577758865152
2213	21	-0.11676090934013672
2213	22	-0.07810454569138159
2213	23	-0.9409231610825728
2213	24	-1.5147510770341555
2213	25	-1.5252092954482421
2214	1	-0.04811522537219304
2214	2	-0.23559234047594302
2214	3	-0.06042591319530999
2214	4	-0.07354018093078452
2214	5	-0.10779963596080222
2214	6	-0.1588518303797457
2214	7	-0.05955945999225776
2214	8	-0.038756368068249
2214	9	-0.4690777228219787
2214	10	-0.03802013225721623
2214	11	-0.22224651273630514
2214	12	-0.21342821481794516
2214	13	-0.17133405981881822
2214	14	-0.053222245417387626
2214	15	-0.184011387550008
2214	16	-0.27551940190608804
2214	17	-0.20815723582799706
2214	18	-0.06839066986220388
2214	19	-0.7669683871500084
2214	20	-0.377018256662474
2214	21	-0.33231449969540705
2214	22	-0.5788360451637828
2214	23	-1.877164940857
2214	24	-1.2822548476980415
2214	25	-1.5625969201816545
2215	1	-0.32633549609607115
2215	2	-0.2555093368689043
2215	3	-1.1816253250054771
2215	4	-0.8790567378008376
2215	5	-0.32813647636861987
2215	6	-0.23614184032012986
2215	7	-0.13147386369301728
2215	8	-0.055120188803428505
2215	9	-0.2807592204071696
2215	10	-1.340227954102076
2215	11	-0.5382520252576768
2215	12	-0.51561458223415
2215	13	-0.4960980768118617
2215	14	-0.7619439297519861
2215	15	-0.9892234577365784
2215	16	-0.6185164199938669
2215	17	-0.4373838712711233
2215	18	-0.31033427854331563
2215	19	-0.8633212498102694
2215	20	-1.2205394134349987
2215	21	-0.5393141342303096
2215	22	-0.2525195767950166
2215	23	-0.5260750325723009
2215	24	-1.7210637768670694
2215	25	-0.7400539686080378
2216	1	-0.38258505316391833
2216	2	-0.33080365684205404
2216	3	-1.2934451448814634
2216	4	-0.49585490725706904
2216	5	-0.5557196411019731
2216	6	-0.2672904712051505
2216	7	-0.24125368095038624
2216	8	-0.3153501186512955
2216	9	-0.15324789121584104
2216	10	-0.20017738969480803
2216	11	-0.2939797484980744
2216	12	-0.1882231590632628
2216	13	-0.9057959892895084
2216	14	-0.5794698588184133
2216	15	-0.9742720114137171
2216	16	-0.6558404759119085
2216	17	-1.5979417008048529
2216	18	-0.6392003410557194
2216	19	-0.4014323896708376
2216	20	-0.3479999288261766
2216	21	-0.17802282983278417
2216	22	-0.2559128371601531
2216	23	-0.0953336290930622
2216	24	-0.21696888079635812
2216	25	-0.12940780203645375
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7905.0
3	762.0
4	1076.0
5	879.0
6	566.0
7	469.0
8	734.0
9	1691.0
10	3486.0
11	6395.0
12	10511.0
13	15323.0
14	20258.0
15	25189.0
16	28699.0
17	30348.0
18	31602.0
19	32816.0
20	33978.0
21	37121.0
22	39811.0
23	42550.0
24	45474.0
25	47804.0
26	51158.0
27	57659.0
28	63982.0
29	75853.0
30	91312.0
31	116018.0
32	152977.0
33	215145.0
34	308270.0
35	485400.0
36	875223.0
37	2945955.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.248102119180615	20.674808487459963	13.8159675305777	23.261121862781724
2	14.931395095902303	20.643419762651092	40.70658317273545	23.718601968711155
3	18.374580851193457	26.183837109512154	29.40338529853645	26.038196740757936
4	12.180374297375538	18.023935274103106	37.90806960427274	31.887620824248618
5	12.856865137071832	38.65402785665715	35.218305560719855	13.270801445551161
6	31.1808671026006	38.389152155334216	17.45375074924024	12.976229992824942
7	26.420051082079034	32.807296411985405	22.847394028273836	17.92525847766173
8	24.57575119268659	36.99754783603787	20.89090255147601	17.53579841979954
9	24.152330561589224	17.620538958227282	21.82916100665419	36.3979694735293
10	14.652401170152324	30.57471943386166	33.68548922508219	21.087390170903824
11	31.890016075074946	25.937282769257507	22.918793717686928	19.253907437980622
12	21.18488197497188	27.804351416061028	31.35666876498745	19.65409784397964
13	26.79299766488117	23.716639431817736	26.36829018277478	23.12207272052632
14	20.32050854479562	23.79898400342569	27.996559372798373	27.88394807898032
15	23.416138833154037	30.457771367350574	23.87283934463621	22.253250454859185
16	21.32531564064435	31.291233884481823	26.354962797222548	21.02848767765128
17	20.470240024710172	30.408023338894253	28.43034395678435	20.691392679611223
18	20.74364005457504	29.83092023161296	29.145439693445653	20.280000020366355
19	21.013969214403318	29.262389024351126	29.43879259444439	20.284849166801166
20	22.209865390486723	28.775925233568728	28.621590234297635	20.39261914164691
21	22.053761772988576	29.128585863351365	28.125465633174567	20.692186730485492
22	21.18913493622288	28.807954493962225	28.55891833382352	21.443992235991377
23	20.879503212692114	29.291276334630496	28.463303145449526	21.365917307227868
24	21.09911766403335	29.16351223354235	28.498602996343685	21.238767106080616
25	21.64979810857378	28.463525537411062	27.590204451031674	22.296471902983487
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11961.0
1	11961.0
2	9638.5
3	7316.0
4	7316.0
5	7316.0
6	9843.5
7	12371.0
8	12371.0
9	12371.0
10	17246.5
11	22122.0
12	22122.0
13	22122.0
14	36469.0
15	50816.0
16	50816.0
17	50816.0
18	82985.5
19	115155.0
20	115155.0
21	115155.0
22	166241.5
23	217328.0
24	217328.0
25	217328.0
26	283063.5
27	348799.0
28	348799.0
29	348799.0
30	417619.5
31	486440.0
32	486440.0
33	486440.0
34	542532.0
35	598624.0
36	598624.0
37	598624.0
38	635949.5
39	673275.0
40	673275.0
41	673275.0
42	675366.0
43	677457.0
44	677457.0
45	677457.0
46	718764.0
47	760071.0
48	760071.0
49	760071.0
50	703370.0
51	646669.0
52	646669.0
53	646669.0
54	567782.0
55	488895.0
56	488895.0
57	488895.0
58	420494.5
59	352094.0
60	352094.0
61	352094.0
62	290971.0
63	229848.0
64	229848.0
65	229848.0
66	175918.0
67	121988.0
68	121988.0
69	121988.0
70	87531.5
71	53075.0
72	53075.0
73	53075.0
74	37025.0
75	20975.0
76	20975.0
77	20975.0
78	13756.5
79	6538.0
80	6538.0
81	6538.0
82	4207.5
83	1877.0
84	1877.0
85	1877.0
86	1201.5
87	526.0
88	526.0
89	526.0
90	322.5
91	119.0
92	119.0
93	119.0
94	71.0
95	23.0
96	23.0
97	23.0
98	30.0
99	37.0
100	37.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3131902163116009
2	0.18286365809627703
3	0.1998848655045162
4	0.2217668555258545
5	0.24734100795017408
6	0.19922434103792783
7	0.19527813076318182
8	0.21793920092459876
9	0.2188537732629519
10	0.2293544186292288
11	0.20403431407667402
12	0.2097757959785577
13	0.22102164843534455
14	0.210859733564754
15	0.20181562932992841
16	0.2048811403158899
17	0.20474564811761536
18	0.20879347754106728
19	0.2070998250626355
20	0.20361090095706605
21	0.2012905970616146
22	0.2046270924441251
23	0.2083192548471064
24	0.22388392112389424
25	0.21790532787503014
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5904399.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.06447930710134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.95219675608861	65.27021374104417
2	9.661952215700492	14.50538824323303
3	1.9462224343616936	4.382765209534792
4	0.594645443182716	1.7854700225940434
5	0.24885173987632395	0.9339963139241242
6	0.11858104382443646	0.5340734586224333
7	0.08513810393240916	0.44735932086161195
8	0.06207459794206373	0.37276778981749426
9	0.0451027839050118	0.304705529021738
>10	0.2494162518807564	3.5220176918812287
>50	0.02058747694937617	1.0606550267624841
>100	0.01287022446422556	1.779152544935967
>500	0.0010669676320765307	0.5370195091814204
>1k	9.534444021387873E-4	1.662038376585731
>5k	2.49711628996929E-4	1.4167584214362
>10k+	9.080422872615598E-5	1.48561880056351
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	31797	0.5385306785669465	No Hit
CCCATGTACTCTGCGTTGATACCAC	30357	0.5141420828775292	No Hit
GAGTACATGGGAAGCAGTGGTATCA	14244	0.24124385902781978	No Hit
CATGTACTCTGCGTTGATACCACTG	10784	0.18264348327408092	No Hit
GCGTTGATACCACTGCTTCCCATGT	9646	0.1633697180695275	No Hit
GTATCAACGCAGAGTACATGGGAAG	9442	0.1599146670135267	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	8368	0.1417248393951696	No Hit
GCTTCCCATGTACTCTGCGTTGATA	7815	0.13235894118944197	No Hit
GGTATCAACGCAGAGTACATGGGAA	7619	0.12903938233171572	No Hit
TATCAACGCAGAGTACATGGGAAGC	7515	0.1272779837541467	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7362	0.1246866954621461	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	7159	0.12124858093092963	No Hit
ACTCTGCGTTGATACCACTGCTTCC	6928	0.11733624370575226	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.693652478431759E-5	0.0
3	0.0	0.0	0.0	1.693652478431759E-5	0.0
4	0.0	0.0	0.0	1.693652478431759E-5	0.0
5	0.0	1.693652478431759E-5	0.0	1.693652478431759E-5	0.0
6	0.0	1.693652478431759E-5	0.0	1.693652478431759E-5	0.0
7	0.0	1.693652478431759E-5	0.0	5.080957435295277E-5	0.0
8	0.0	1.693652478431759E-5	0.0	5.080957435295277E-5	0.0
9	0.0	1.693652478431759E-5	0.0	6.774609913727036E-5	0.0
10	0.0	1.693652478431759E-5	0.0	8.468262392158795E-5	0.0
11	0.0	1.693652478431759E-5	0.0	1.3549219827454072E-4	0.0
12	0.0	1.693652478431759E-5	0.0	1.3549219827454072E-4	0.0
13	0.0	1.693652478431759E-5	0.0	1.524287230588583E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGACCG	155	3.6379788E-9	10.42515	5
AGTGCGA	120	1.5278601E-6	10.29066	10
CCGTAGA	125	2.5586542E-6	9.884487	2
TAGCCCG	110	6.801897E-5	9.505284	5
TACACCG	130	4.2142583E-6	9.505283	5
GCACCGT	130	4.2531647E-6	9.4984255	6
GTCCTAA	475	0.0	9.410105	1
CTAGGAC	630	0.0	9.353453	3
ACCGTCT	165	1.0690019E-7	9.211298	8
GCCGTAG	85	0.0073746187	8.950788	1
TAGGACT	805	0.0	8.856155	4
GTCCTAT	465	0.0	8.79439	1
GTATACG	185	6.473056E-8	8.739113	1
GTCTAGG	730	0.0	8.728551	1
GACAGCG	110	7.156748E-4	8.635519	7
CTTACAC	860	0.0	8.509678	3
TAGGACA	715	0.0	8.508514	4
TAGACAG	1070	0.0	8.439271	5
GTATTAG	815	0.0	8.40166	1
GTCCTAC	365	0.0	8.33772	1
>>END_MODULE