FastQCFastQC Report
Wed 25 May 2016
SRR1294973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294973_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5904399
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC339540.5750627625267195No Hit
GTACATGGGAAGCAGTGGTATCAAC306840.5196803264820009No Hit
GTATCAACGCAGAGTACTTTTTTTT196370.33258253718964453No Hit
GGTATCAACGCAGAGTACTTTTTTT159010.26930768059543403No Hit
GAGTACATGGGAAGCAGTGGTATCA152520.2583158760104119No Hit
TATCAACGCAGAGTACTTTTTTTTT126890.2149075629882059No Hit
CATGTACTCTGCGTTGATACCACTG123020.20835312789667498No Hit
GCGTTGATACCACTGCTTCCCATGT104960.17776576413619743No Hit
ACGCAGAGTACATGGGAAGCAGTGG100880.17085566202419586No Hit
GTATCAACGCAGAGTACATGGGAAG97460.16506337054795925No Hit
GCTTCCCATGTACTCTGCGTTGATA86360.14626382803736673No Hit
TATCAACGCAGAGTACATGGGAAGC83380.14121674365164008No Hit
GGTATCAACGCAGAGTACATGGGAA82220.13925210677665922No Hit
ACTCTGCGTTGATACCACTGCTTCC80320.1360341670676389No Hit
ACGCAGAGTACTTTTTTTTTTTTTT74620.12638034794057787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTC951.64674E-49.999318
TATTCCG2107.2759576E-129.9516945
GCGTAGG1151.0123071E-59.9256391
CGTAGAC1151.0285434E-59.9123593
CTAAGAC5850.09.9052983
ACCGTTT1356.459031E-79.85117158
TTACGCT1002.7596878E-49.4993444
CACCGTT1252.7593465E-59.11936957
TGGACCG1151.1125267E-49.0863295
GTCCTAG5800.09.0200671
GTATTAG7750.08.8370211
TACACCG1304.3768465E-58.7686255
TAGGACC3500.08.6851154
GTGCTAG7450.08.6821581
GTCTTAG5700.08.6776781
TGTACCG1107.1547076E-48.6357675
GAGCCGT1107.15531E-48.63569456
GGACAGT7700.08.63569456
TAGGACA7850.08.5917634
TACCGTT1000.00290871238.5494097
Produced by FastQC (version 0.11.5)