FastQCFastQC Report
Wed 25 May 2016
SRR1294972_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294972_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616543
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1152717.130710411043814No Hit
CCCATGTACTCTGCGTTGATACCAC1123776.9516864073519855No Hit
GAGTACATGGGAAGCAGTGGTATCA534923.3090366293998983No Hit
CATGTACTCTGCGTTGATACCACTG439832.720806065783589No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA368702.280793025610825No Hit
GCGTTGATACCACTGCTTCCCATGT361922.2388516729836447No Hit
ACGCAGAGTACATGGGAAGCAGTGG329062.0355783916666614No Hit
TATCAACGCAGAGTACATGGGAAGC322901.9974723839699904No Hit
GGTATCAACGCAGAGTACATGGGAA303781.8791952951452573No Hit
GTATCAACGCAGAGTACATGGGAAG302491.8712153032737144No Hit
ACTCTGCGTTGATACCACTGCTTCC294131.8195000071139462No Hit
GCTTCCCATGTACTCTGCGTTGATA194681.2042983081798628No Hit
GTACTCTGCGTTGATACCACTGCTT163641.012283620045987No Hit
GTGGTATCAACGCAGAGTACATGGG162201.003375722142869No Hit
ATACCACTGCTTCCCATGTACTCTG147570.912873953863275No Hit
GATACCACTGCTTCCCATGTACTCT139050.8601688912698271No Hit
GGGAAGCAGTGGTATCAACGCAGAG134130.829733573434174No Hit
ACATGGGAAGCAGTGGTATCAACGC128900.7973805831332664No Hit
CATGGGAAGCAGTGGTATCAACGCA126790.7843280382891146No Hit
CAGTGGTATCAACGCAGAGTACATG125800.7782038584807208No Hit
TACCACTGCTTCCCATGTACTCTGC121260.7501192359250574No Hit
GTTGATACCACTGCTTCCCATGTAC117610.7275401891567376No Hit
CTGCTTCCCATGTACTCTGCGTTGA116300.7194364764809844No Hit
GCAGTGGTATCAACGCAGAGTACAT114710.7096006725462917No Hit
GCAGAGTACATGGGAAGCAGTGGTA109740.6788560527001138No Hit
CTTCCCATGTACTCTGCGTTGATAC107630.6658035078559618No Hit
CCATGTACTCTGCGTTGATACCACT106580.6593081656349382No Hit
CCACTGCTTCCCATGTACTCTGCGT96930.599612877603627No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA88070.5448045613386095No Hit
AAGCAGTGGTATCAACGCAGAGTAC87170.5392371251491609No Hit
GTACATGGTAAGCAGTGGTATCAAC84520.5228441185913397No Hit
ATGGGAAGCAGTGGTATCAACGCAG83180.5145548247092716No Hit
TTCCCATGTACTCTGCGTTGATACC82820.5123278502334921No Hit
TGATACCACTGCTTCCCATGTACTC74570.4612930184968788No Hit
ATCAACGCAGAGTACATGGGAAGCA67840.41966096788022345No Hit
ACCATGTACTCTGCGTTGATACCAC66120.40902097871816584No Hit
AAACAAAAAAAAAAAAAAAAAAAAA59970.3709768314235996No Hit
GTATCAACGCAGAGTACTTTTTTTT56320.3483977846552798No Hit
GAACAAAAAAAAAAAAAAAAAAAAA49660.3071987568533593No Hit
GGTATCAACGCAGAGTACTTTTTTT43840.27119600282825757No Hit
GGAAGCAGTGGTATCAACGCAGAGT42230.2612364780893549No Hit
CCCCATGTACTCTGCGTTGATACCA41660.2577104351693707No Hit
AAAAAGTACTCTGCGTTGATACCAC40540.2507820701336123No Hit
TATCAACGCAGAGTACTTTTTTTTT39730.24577137756310843No Hit
TCCATGTACTCTGCGTTGATACCAC38580.23865743132103506No Hit
GGTAAGCAGTGGTATCAACGCAGAG38380.23742022327893536No Hit
AAAAACAAAAAAAAAAAAAAAAAAA35420.21910954425585957No Hit
TCAACGCAGAGTACATGGGAAGCAG33810.20915001951695686No Hit
CTCTGCGTTGATACCACTGCTTCCC31860.19708724110648465No Hit
CTGCGTTGATACCACTGCTTCCCAT31530.19504584783702011No Hit
TCCCATGTACTCTGCGTTGATACCA30530.18885980762652152No Hit
TGGGAAGCAGTGGTATCAACGCAGA29910.18502446269601242No Hit
AAAGTACTCTGCGTTGATACCACTG29910.18502446269601242No Hit
GAGTACATGGTAAGCAGTGGTATCA29720.18384911505601767No Hit
GTACATGGGTAAGCAGTGGTATCAA29590.1830449298286529No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28630.17710633122657424No Hit
GTACATGGAAAGCAGTGGTATCAAC27610.1707965702118657No Hit
AGTGGTATCAACGCAGAGTACATGG27450.1698068037781859No Hit
GCTTACCATGTACTCTGCGTTGATA26130.16164123070032782No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA23930.14803194223723093No Hit
AACGCAGAGTACATGGGAAGCAGTG22700.14042311277831768No Hit
ATGTACTCTGCGTTGATACCACTGC22290.13788683629201326No Hit
ACTCTGCGTTGATACCACTGCTTAC22110.1367733490541235No Hit
TTGATACCACTGCTTCCCATGTACT22010.13615474503307368No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA21320.13188637728782965No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21160.13089661085414986No Hit
GCGTTGATACCACTGCTTACCATGT21150.1308347504520449No Hit
ACTGCTTCCCATGTACTCTGCGTTG20240.12520545386049117No Hit
ACGCAGAGTACATGGTAAGCAGTGG20100.12433940823102138No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN19540.12087522571314219No Hit
GAGTACATGGAAAGCAGTGGTATCA19310.1194524364647275No Hit
ACACAAAAAAAAAAAAAAAAAAAAA19310.1194524364647275No Hit
TATCAACGCAGAGTACATGGTAAGC19280.11926685525841255No Hit
CTGCTTACCATGTACTCTGCGTTGA18540.1146891855026436No Hit
GGTATCAACGCAGAGTACATGGTAA18030.11153430499528934No Hit
GTATCAACGCAGAGTACATGGTAAG17020.10528640438268577No Hit
GAAAACAAAAAAAAAAAAAAAAAAA16830.10411105674269104No Hit
CAACGCAGAGTACATGGGAAGCAGT16190.10015199100797195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA6350.016.9241811
ACTTGTG350.002164600416.2920685
TACATAA5950.016.2905522
GTACATA7950.014.8339731
TACATGA4500.014.7821672
AGAACAA10200.014.72005752
TGCGCTA400.005280760614.249372510
GCGCTAT400.00528728114.24672211
TCGAAAC400.00528836814.24628116
GCTATCG551.9635362E-413.81543313
TTGTGCG500.001500438313.2990037
TACAAAA9000.013.1983632
ACATAAA10550.012.7004543
CCCGTGT604.054252E-412.6806541
CCCAAGT604.054252E-412.6806541
CCTCTGG604.054252E-412.6806541
GAGAACA13850.012.2915361
CCAAGTA701.0879292E-412.2179142
TTTGCGT550.003069543712.09037710
CGCTATC550.00307310912.08850412
Produced by FastQC (version 0.11.5)