##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294972_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1616543
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.555738387410667	33.0	27.0	33.0	15.0	33.0
2	29.56857318363941	33.0	27.0	33.0	15.0	33.0
3	29.890901139035584	33.0	27.0	33.0	22.0	33.0
4	32.717160632287545	37.0	33.0	37.0	15.0	37.0
5	33.886936505864675	37.0	33.0	37.0	27.0	37.0
6	33.25249189164779	37.0	33.0	37.0	22.0	37.0
7	33.55299982740948	37.0	33.0	37.0	22.0	37.0
8	33.33665420591967	37.0	33.0	37.0	22.0	37.0
9	33.04896312687012	37.0	33.0	37.0	15.0	37.0
10	33.38784368866155	37.0	33.0	37.0	22.0	37.0
11	33.4716292730846	37.0	33.0	37.0	22.0	37.0
12	33.62278454702411	37.0	33.0	37.0	22.0	37.0
13	33.15170211989412	37.0	33.0	37.0	15.0	37.0
14	34.576373780344845	37.0	33.0	40.0	22.0	40.0
15	34.46933672658259	37.0	33.0	40.0	15.0	40.0
16	34.49020409602466	37.0	33.0	40.0	22.0	40.0
17	34.59075384941817	37.0	33.0	40.0	22.0	40.0
18	34.42518139016407	37.0	33.0	40.0	22.0	40.0
19	34.290154360261376	37.0	33.0	40.0	15.0	40.0
20	34.37910281384411	37.0	33.0	40.0	22.0	40.0
21	34.29862799814171	37.0	33.0	40.0	22.0	40.0
22	34.37286109927172	37.0	33.0	40.0	22.0	40.0
23	34.361123087972295	37.0	33.0	40.0	22.0	40.0
24	34.54380304142853	37.0	33.0	40.0	22.0	40.0
25	33.35241623637602	37.0	33.0	40.0	15.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5383546860874944
1101	2	0.5770452775980068
1101	3	0.5005545820821773
1101	4	0.4850794136606851
1101	5	0.44650217984418106
1101	6	0.3938857835620482
1101	7	0.3104343588009968
1101	8	0.27843083758858
1101	9	0.4344434119812419
1101	10	0.753096270720846
1101	11	0.3151229659058572
1101	12	0.454344562554887
1101	13	0.4352073085956647
1101	14	0.9268661022707292
1101	15	0.7735664558149438
1101	16	0.7292002384893834
1101	17	0.5773137993314208
1101	18	0.7093012964096204
1101	19	0.7792166397373208
1101	20	0.8066725069731646
1101	21	0.5493615165715511
1101	22	0.6955566556077386
1101	23	0.8799157371970097
1101	24	0.7189339315558527
1101	25	0.7115222564242529
1102	1	0.7913115805345008
1102	2	0.7157254356607439
1102	3	0.7156189194032478
1102	4	0.9438773049518616
1102	5	0.5370740269537464
1102	6	0.6167266628538428
1102	7	0.5625050891629684
1102	8	0.7179591812755675
1102	9	0.6557968015588713
1102	10	0.7929849576607779
1102	11	0.7468069489421438
1102	12	0.7053211515465563
1102	13	0.6109164659259605
1102	14	1.2616191066382498
1102	15	1.2117768564877096
1102	16	0.9710552150241156
1102	17	0.9765397621306207
1102	18	1.0601815828757282
1102	19	0.9663396809077796
1102	20	0.8717490516566286
1102	21	1.0396408391081522
1102	22	0.9685517093653431
1102	23	1.0863105865943297
1102	24	0.9333765976438002
1102	25	1.0660466666277983
1103	1	0.3093828995079946
1103	2	0.23311814681245835
1103	3	0.358302985369491
1103	4	0.2842543971429663
1103	5	0.07765140727865827
1103	6	0.10251469710194527
1103	7	0.10373106337147675
1103	8	0.1300959394188581
1103	9	0.3182651771166931
1103	10	0.36780384916551867
1103	11	0.23331824589565997
1103	12	0.24446957559518978
1103	13	0.29983736968224406
1103	14	0.6288154827114312
1103	15	0.5894620058839166
1103	16	0.4477658630909289
1103	17	0.3255352468630406
1103	18	0.38969143330382394
1103	19	0.4247659842447433
1103	20	0.36479313847158323
1103	21	0.4732119413956539
1103	22	0.39200665039161464
1103	23	0.43528703809766256
1103	24	0.6194132351457
1103	25	0.5570417531265051
1104	1	0.6061318584944502
1104	2	0.5098462222659066
1104	3	0.571200271564571
1104	4	0.5368337348868479
1104	5	0.6172545568893852
1104	6	0.5402630009738658
1104	7	0.35259023624017516
1104	8	0.5069372189447066
1104	9	0.6034791647960347
1104	10	0.9080647492310305
1104	11	0.5864049459459864
1104	12	0.5609127394890976
1104	13	0.5162451770729461
1104	14	1.062513604247826
1104	15	0.9273882872232733
1104	16	0.9165772800783571
1104	17	0.8886383458640594
1104	18	0.9814097523305492
1104	19	1.027856726042458
1104	20	1.0529189064035833
1104	21	0.801872780448349
1104	22	0.8949696026807246
1104	23	1.0454469717989454
1104	24	1.0648932260359558
1104	25	1.0485131389608426
1105	1	0.28958353975960094
1105	2	0.30010552430817583
1105	3	0.1655714557859369
1105	4	0.28752375211755776
1105	5	0.10822271951364115
1105	6	0.1455316019908821
1105	7	0.1907828170430932
1105	8	0.22174458730855662
1105	9	0.29634533493309334
1105	10	0.4975969608444899
1105	11	0.29467218147735963
1105	12	0.17446943518913827
1105	13	0.3111891991731284
1105	14	0.6080936551862308
1105	15	0.4506586866847897
1105	16	0.4415339403704195
1105	17	0.40555792244081346
1105	18	0.2419354730668246
1105	19	0.34909870447163627
1105	20	0.5196518899809419
1105	21	0.5033486968925516
1105	22	0.3376740705707775
1105	23	0.5498910228214839
1105	24	0.5004682462377801
1105	25	0.6864494501864016
1106	1	0.2514050563527128
1106	2	0.038341324159151924
1106	3	-0.018112463130400158
1106	4	0.187460704029057
1106	5	-0.26121424195059717
1106	6	-0.3268017504479346
1106	7	-0.7005623998996455
1106	8	-0.12209676077420539
1106	9	-0.06975963058150825
1106	10	-0.311182007841623
1106	11	-0.5753092862057159
1106	12	-0.9010201427707827
1106	13	-0.28474324384477256
1106	14	-0.3874796047820581
1106	15	-0.2891112833196061
1106	16	-0.035073308249209845
1106	17	-0.3334688443385758
1106	18	-0.4942866090485367
1106	19	-0.4429744313110149
1106	20	0.12919125682529398
1106	21	0.19138484446328619
1106	22	0.3824057340850331
1106	23	-0.48444534828338703
1106	24	0.13144014207469468
1106	25	0.20809405002209047
1107	1	-1.0906927473965595
1107	2	-1.081387864879762
1107	3	-0.9449240928839941
1107	4	-0.938588497656422
1107	5	-0.927757713614838
1107	6	-1.1569665373914404
1107	7	-1.076072214616545
1107	8	-0.9103474462508245
1107	9	-1.071448746720968
1107	10	-0.8825268139366855
1107	11	-0.9245918297646014
1107	12	-0.91229811830258
1107	13	-0.8472721574211164
1107	14	-1.1307512134216964
1107	15	-0.9469122706966573
1107	16	-1.0089501532215621
1107	17	-1.057089033055881
1107	18	-1.1775349959753285
1107	19	-1.3186336063405903
1107	20	-1.4064297241194552
1107	21	-1.3011306048804911
1107	22	-1.4477783134340712
1107	23	-1.2477906558802303
1107	24	-0.9880233963837668
1107	25	-0.9128689060765112
1108	1	-0.22678735429128238
1108	2	-0.21576039210600229
1108	3	-0.31845361675450334
1108	4	-0.3372073272521021
1108	5	-0.7899866137555662
1108	6	-0.3942189550876236
1108	7	-0.40915947122699947
1108	8	-0.37928460520815577
1108	9	-0.6989623808045735
1108	10	-0.8427675849160963
1108	11	-0.517019253140063
1108	12	-0.24811508844862828
1108	13	-0.33428924709885166
1108	14	-0.4725055849426596
1108	15	-0.27179117624984883
1108	16	-1.0058810550607333
1108	17	-1.0987656479915486
1108	18	-0.8974672105286174
1108	19	-0.35856252767379715
1108	20	-1.2252831041677297
1108	21	-0.38859731057791436
1108	22	-0.562731514642195
1108	23	-0.3168077059173555
1108	24	-0.5534441281189757
1108	25	-1.143451638320954
1109	1	0.07882804421873857
1109	2	0.05459906212625398
1109	3	0.1432537945200565
1109	4	0.030906731216298056
1109	5	0.06402697610212016
1109	6	-0.07077388064004708
1109	7	-0.015849840109247282
1109	8	0.09943670872284827
1109	9	0.14365197063731472
1109	10	0.1425097266006219
1109	11	-0.013770111133830198
1109	12	0.015737270538608072
1109	13	0.060438258191560124
1109	14	0.45932763604745475
1109	15	0.20780333082777247
1109	16	0.27373900459130596
1109	17	0.36905088452410695
1109	18	0.2959283301065838
1109	19	0.2458928720697955
1109	20	0.19412069846653424
1109	21	0.29402708566909297
1109	22	0.3038282424027514
1109	23	0.4371583032845052
1109	24	0.25416040614130253
1109	25	0.44422644036041703
1110	1	-0.10646458289440375
1110	2	-0.11981381038372163
1110	3	-0.13164013902722616
1110	4	-1.0802443767583938
1110	5	-0.42064270265941417
1110	6	-0.28457365256677036
1110	7	-0.1765931407985164
1110	8	-0.0916943410086759
1110	9	-0.05126605122496386
1110	10	-0.1962001063572174
1110	11	-0.34119559906397967
1110	12	-0.07977586339348619
1110	13	-0.2102694382524959
1110	14	0.13842107714874885
1110	15	-0.0043821094726865795
1110	16	-0.12192423512297523
1110	17	0.058100042332647206
1110	18	0.16293264962391873
1110	19	0.05026987550125739
1110	20	0.0316077644547903
1110	21	0.24292591892012183
1110	22	-0.4501830823869284
1110	23	0.008330163250448663
1110	24	0.1521014280552464
1110	25	0.14067840830925604
1111	1	-0.1774367495339142
1111	2	-0.4327176341720502
1111	3	-0.19987081206523527
1111	4	-0.197495356003472
1111	5	-0.315101999801648
1111	6	-0.25345627692956185
1111	7	-0.22575703502911182
1111	8	-0.07678488419487195
1111	9	-0.20265816796202785
1111	10	-0.2565160504891395
1111	11	-0.1721487458992783
1111	12	-0.19866576531332925
1111	13	-0.29336175217068927
1111	14	-0.09326563061063808
1111	15	-0.22355394311137644
1111	16	-0.3303597791042421
1111	17	-0.1612251949171153
1111	18	-0.29354251119045927
1111	19	-0.21765191501895487
1111	20	-0.42441209332015006
1111	21	-0.38253285463063236
1111	22	-0.32415780921211734
1111	23	-0.21808389132477402
1111	24	-0.15603398330340923
1111	25	-0.044550702928553676
1112	1	-0.20144965817583582
1112	2	-0.49999984172425727
1112	3	-0.22622831821156097
1112	4	-0.45445010489005
1112	5	-0.23516518019010846
1112	6	-0.19562986763621382
1112	7	-0.3149359625433803
1112	8	-0.15224374607567626
1112	9	-0.310462226988534
1112	10	-0.03163829554618758
1112	11	-0.18746140432723735
1112	12	-0.15169340877751125
1112	13	-0.16083230160566586
1112	14	-0.515890097066162
1112	15	-0.2951740103802294
1112	16	-0.43352179673369307
1112	17	-0.44377781344167033
1112	18	-0.42044732631014625
1112	19	-0.4002187068834573
1112	20	-0.9238917682055359
1112	21	-1.4633113525836734
1112	22	-1.7386704916257258
1112	23	-0.7236147646750837
1112	24	-0.6141119693808292
1112	25	-1.779135560350202
1113	1	-0.5370253429784526
1113	2	-0.45239444267103934
1113	3	-0.4311534693302619
1113	4	-0.9330363097560586
1113	5	-0.5196501988927267
1113	6	-0.5658205037812962
1113	7	-0.4740151177635994
1113	8	-0.37406602894735386
1113	9	-0.5592861765823542
1113	10	-0.4116311012463214
1113	11	-0.32145971968336085
1113	12	-0.4189968520564733
1113	13	-0.6231067756664999
1113	14	-0.8074205371473724
1113	15	-0.8134111764917478
1113	16	-0.9221999913484638
1113	17	-0.9954802510548006
1113	18	-1.2766747481137344
1113	19	-1.3879617705286904
1113	20	-1.4333187332606983
1113	21	-1.4247456809151302
1113	22	-0.8959170205680635
1113	23	-2.1314178917632844
1113	24	-1.3748034414472272
1113	25	-0.9111674764149953
1114	1	-0.5118691091884813
1114	2	-0.6723088784262217
1114	3	-0.7035825898266523
1114	4	-0.8816450262429854
1114	5	-0.9227101580866872
1114	6	-0.7567843859871957
1114	7	-0.5487053672930458
1114	8	-0.6244602568189705
1114	9	-1.5322700814143637
1114	10	-0.7604986841552162
1114	11	-0.8345237051394108
1114	12	-0.7230746157272421
1114	13	-0.6966604089357773
1114	14	-1.1172805566117532
1114	15	-1.70709957838681
1114	16	-1.552574433723514
1114	17	-1.7588595447051603
1114	18	-2.087911601009438
1114	19	-1.9242497544829291
1114	20	-1.2160013600632524
1114	21	-0.9320555697112596
1114	22	-0.749179898431521
1114	23	-0.5194074037210328
1114	24	-1.126772604725744
1114	25	-0.745206805338384
1115	1	-0.8843354673663057
1115	2	-0.7963730333051728
1115	3	-0.6492506673066281
1115	4	-0.8132847929525155
1115	5	-0.5124490226182346
1115	6	-0.6808222146847598
1115	7	-0.5507519747595566
1115	8	-0.4005758287552581
1115	9	-0.9705313890155409
1115	10	-0.5089153162265774
1115	11	-0.7567913512750337
1115	12	-0.9218096933388509
1115	13	-0.761024884791631
1115	14	-0.6376005715974316
1115	15	-1.6700930677196482
1115	16	-1.2664642789053175
1115	17	-0.7934936614111479
1115	18	-2.2572307148296673
1115	19	-1.4351806887585994
1115	20	-0.8520327894736468
1115	21	-1.1424911161314029
1115	22	-0.8269147836594968
1115	23	-0.5226807099856572
1115	24	-0.6317760980075064
1115	25	-0.7905984579137879
1116	1	-1.8152167113189464
1116	2	-1.184820168475106
1116	3	-1.9768284583530047
1116	4	-1.3604663998792823
1116	5	-1.33740446461767
1116	6	-0.6879914495891768
1116	7	-1.5190084332597777
1116	8	-3.1984044661871103
1116	9	-1.982253796374156
1116	10	-1.3399402578101203
1116	11	-1.155904047043144
1116	12	-1.1103538097409142
1116	13	-2.0757465855367734
1116	14	-2.2266228325761332
1116	15	-2.1704711017359983
1116	16	-1.451056640606481
1116	17	-2.126852187698759
1116	18	-2.8993903647696087
1116	19	-1.851802674021144
1116	20	-1.4929444801127403
1116	21	-1.1830859064147248
1116	22	-0.9565857599512029
1116	23	-0.8525244698957763
1116	24	-0.9625041725235093
1116	25	-0.8603859681504673
1201	1	0.6937586227202139
1201	2	0.5672855849054912
1201	3	0.5330310575749984
1201	4	0.6924382666176285
1201	5	0.6506264130643586
1201	6	0.44103008705796753
1201	7	0.3701508736005863
1201	8	0.39844527984931943
1201	9	0.5555395349042413
1201	10	0.5993301162333253
1201	11	0.5426071836415503
1201	12	0.6580630724709096
1201	13	0.7009597950467423
1201	14	0.9690174035630861
1201	15	0.8101053754742864
1201	16	0.7951246920656985
1201	17	0.7985044669509804
1201	18	0.810722444643794
1201	19	0.7427305757744804
1201	20	0.8032130644146491
1201	21	1.0197839154757276
1201	22	1.06260373439995
1201	23	1.0401846312473921
1201	24	0.9261535730304473
1201	25	1.1005617716407343
1202	1	0.6363389240172168
1202	2	0.5452472987921944
1202	3	0.5953119189754084
1202	4	0.720685046394479
1202	5	0.36042302273023097
1202	6	0.32008935104941116
1202	7	0.34476348112707456
1202	8	0.4631752936661897
1202	9	0.555265797365422
1202	10	0.6191359742909412
1202	11	0.3530179385183132
1202	12	0.35892606998982046
1202	13	0.33522118653970523
1202	14	0.774017663558034
1202	15	0.630724089167849
1202	16	0.45805888362998104
1202	17	0.7108566412515955
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2210	20	0.3202757621293628
2210	21	0.4776227706008882
2210	22	0.48236749403135804
2210	23	0.34529567964401764
2210	24	0.4424889103104235
2210	25	0.6109123419779507
2211	1	0.12603099444513788
2211	2	-0.0879607768545334
2211	3	0.12922832445814336
2211	4	-0.08075460939956969
2211	5	0.137737775415971
2211	6	0.006358021641958089
2211	7	0.024739591441438336
2211	8	0.18427208385104876
2211	9	0.24653858837026377
2211	10	0.3152001715792707
2211	11	-0.01785842562397022
2211	12	0.07133574656086239
2211	13	-0.11010807932716915
2211	14	0.007102742803489548
2211	15	-0.015369863459810063
2211	16	-0.06492549395451874
2211	17	0.13839282195380775
2211	18	-0.06384027637846401
2211	19	0.0801283632513119
2211	20	0.07974214404181623
2211	21	0.075919789113442
2211	22	0.020147905985524517
2211	23	0.19433787772747024
2211	24	0.1989298755006388
2211	25	0.019614547483087108
2212	1	0.2068236421691374
2212	2	0.1215377705645686
2212	3	0.22437863052410734
2212	4	0.18647491940113525
2212	5	0.3003818944096466
2212	6	0.21346139044871393
2212	7	0.27698864078881513
2212	8	0.30673843332698425
2212	9	0.16829046357483435
2212	10	0.2970545605943897
2212	11	0.24445311187438534
2212	12	0.24319855831885206
2212	13	0.19125409294675677
2212	14	0.06740286126948547
2212	15	0.14658962246939922
2212	16	0.233246932632035
2212	17	0.17022238247635357
2212	18	0.2753155493428068
2212	19	0.2937594614948438
2212	20	0.21940148135960413
2212	21	0.23122266699224525
2212	22	-0.20712676351757864
2212	23	0.2743037387230345
2212	24	0.16983704957291934
2212	25	0.07342911245346073
2213	1	0.017271206768082692
2213	2	-0.672306358836412
2213	3	-0.1091540327957361
2213	4	-0.05519374251197462
2213	5	0.08569519352207067
2213	6	0.08892349277014233
2213	7	0.032775017700274134
2213	8	0.26614545257154276
2213	9	-0.01576235887159072
2213	10	-1.337892294475452
2213	11	-0.39098960249132375
2213	12	0.06483702225809651
2213	13	0.06923442338552377
2213	14	0.15422729191050166
2213	15	0.09619352549587745
2213	16	-0.007604164883282749
2213	17	0.0637865285141146
2213	18	0.02408605577203815
2213	19	0.1672100958534415
2213	20	0.14089349745394486
2213	21	0.0461417897586216
2213	22	0.24153856260301865
2213	23	-0.6677929765497765
2213	24	-1.2122786643392374
2213	25	-1.1819632113954768
2214	1	-0.35639091985543914
2214	2	-0.5923731061571402
2214	3	-0.2987335448176829
2214	4	-0.22215532944148464
2214	5	-0.18589716188286332
2214	6	-0.2152682943635611
2214	7	-0.21514117425303425
2214	8	-0.12075774618868707
2214	9	-0.6769200006341123
2214	10	-0.2662487015006647
2214	11	-0.3972679043246714
2214	12	-0.28283242365857575
2214	13	-0.3110036110053329
2214	14	-0.2552172903144623
2214	15	-0.2889621825834183
2214	16	-0.5004857935078348
2214	17	-0.3477921551461307
2214	18	-0.252498878368165
2214	19	-0.9256279745421949
2214	20	-0.8080026287591693
2214	21	-0.7286238959246916
2214	22	-0.8738582641925916
2214	23	-2.1484677262473255
2214	24	-1.6601764762306317
2214	25	-1.9880993824760829
2215	1	-0.8085637535126118
2215	2	-0.5758463364844815
2215	3	-1.2237010164793283
2215	4	-1.1396179162888096
2215	5	-0.4216322924388294
2215	6	-0.5965713862742348
2215	7	-0.4144930307297443
2215	8	-0.362138540152138
2215	9	-0.6708102118667867
2215	10	-1.514548103651812
2215	11	-0.7097721388194955
2215	12	-0.7130588288061332
2215	13	-0.751786951748521
2215	14	-0.9665597525180445
2215	15	-1.3852304861100322
2215	16	-1.167434677406277
2215	17	-0.8590842237704166
2215	18	-0.7517454473764644
2215	19	-1.4108100862557151
2215	20	-1.8039854840623448
2215	21	-1.1033632117688015
2215	22	-0.52887341441145
2215	23	-0.8940976039526447
2215	24	-1.9552340975053326
2215	25	-0.8994239918022089
2216	1	-0.6786324843578271
2216	2	-0.7436490793413313
2216	3	-1.5040152219168341
2216	4	-0.8937913159595041
2216	5	-0.5883935506380027
2216	6	-0.8301132886226128
2216	7	-0.6098854240400655
2216	8	-0.9080202373920656
2216	9	-0.6028542944968507
2216	10	-0.6828159817703039
2216	11	-0.7394027982096318
2216	12	-0.5401901035726411
2216	13	-1.3583868474214995
2216	14	-0.6711141753842824
2216	15	-1.1701577824204534
2216	16	-0.790515053850811
2216	17	-1.6556924477495656
2216	18	-1.0791369019986732
2216	19	-0.880704088472342
2216	20	-0.8073359374002322
2216	21	-0.6100935799870655
2216	22	-0.566683924402227
2216	23	-0.5778280729268346
2216	24	-0.6796994215249015
2216	25	-0.5654318762721502
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2172.0
3	262.0
4	263.0
5	220.0
6	160.0
7	123.0
8	172.0
9	321.0
10	681.0
11	1487.0
12	2996.0
13	5300.0
14	9252.0
15	15717.0
16	21883.0
17	23739.0
18	22529.0
19	21590.0
20	21113.0
21	21487.0
22	21586.0
23	20472.0
24	20005.0
25	20026.0
26	19233.0
27	19457.0
28	20321.0
29	21376.0
30	24986.0
31	31219.0
32	40917.0
33	55832.0
34	77857.0
35	123054.0
36	232569.0
37	696166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.54516345223025	21.084841263931292	10.472919303993844	21.897075979844615
2	10.546591033742919	29.374689743260806	30.64273731193251	29.435981911063763
3	22.75062713733424	36.88379534154888	23.278308889388903	17.087268631727973
4	13.264209128412553	27.74569699344428	23.195068390555893	35.79502548758728
5	9.283011285623388	47.033415318134516	31.095805627803884	12.587767768438207
6	30.17396554160768	31.511899773632805	18.277477084350913	20.036657600408603
7	19.199938018129696	41.14598280003099	20.36538312543581	19.288696056403502
8	27.451855626706045	38.80309706121402	19.51806960018301	14.226977711896929
9	26.32841705879071	25.329849163985347	16.20392929988035	32.1378044773436
10	17.76517497701301	39.665899710391855	25.69625927462181	16.872666037973325
11	22.42894024813188	35.739607191671496	13.31258852444282	28.518864035753804
12	23.822718802652673	41.58332124530272	22.12481542507095	12.469144526973665
13	29.196487469063243	27.711709097762633	17.601961105242015	25.48984232793211
14	19.995883834877308	38.23402878340045	20.381216401182282	21.388870980539963
15	37.10924715613254	25.641791710882565	17.46697444238379	19.781986690601105
16	14.241969486497158	33.51575977233578	33.23799800775204	19.004272733415032
17	26.617217341067693	37.386764722290756	22.52432071230832	13.471697224333234
18	26.9361160581729	28.308923121936587	26.460474922064403	18.29448589782611
19	21.991677581601664	39.46311537143116	27.052136920085317	11.493070126881857
20	23.718350438523903	31.769040565862888	23.653636472394595	20.858972523218615
21	16.705087334444528	36.0401566745037	28.90209455141417	18.352661439637608
22	21.025523408080087	35.012784949402594	15.055990329929179	28.90570131258814
23	14.309775058781524	35.86314458983732	25.05837859081799	24.76870176056316
24	20.026932135853528	44.96048527443313	17.838634099283112	17.173948490430227
25	20.512354752536076	29.09146366790802	16.086441420860087	34.30974015869582
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	43351.0
1	43351.0
2	39197.5
3	35044.0
4	35044.0
5	35044.0
6	35089.0
7	35134.0
8	35134.0
9	35134.0
10	31652.5
11	28171.0
12	28171.0
13	28171.0
14	25994.0
15	23817.0
16	23817.0
17	23817.0
18	23425.0
19	23033.0
20	23033.0
21	23033.0
22	24471.5
23	25910.0
24	25910.0
25	25910.0
26	28610.0
27	31310.0
28	31310.0
29	31310.0
30	33365.5
31	35421.0
32	35421.0
33	35421.0
34	36341.5
35	37262.0
36	37262.0
37	37262.0
38	40661.5
39	44061.0
40	44061.0
41	44061.0
42	74330.5
43	104600.0
44	104600.0
45	104600.0
46	345032.0
47	585464.0
48	585464.0
49	585464.0
50	483303.5
51	381143.0
52	381143.0
53	381143.0
54	246929.5
55	112716.0
56	112716.0
57	112716.0
58	71165.0
59	29614.0
60	29614.0
61	29614.0
62	24449.5
63	19285.0
64	19285.0
65	19285.0
66	15219.0
67	11153.0
68	11153.0
69	11153.0
70	8588.5
71	6024.0
72	6024.0
73	6024.0
74	4352.0
75	2680.0
76	2680.0
77	2680.0
78	1826.5
79	973.0
80	973.0
81	973.0
82	628.0
83	283.0
84	283.0
85	283.0
86	178.0
87	73.0
88	73.0
89	73.0
90	41.0
91	9.0
92	9.0
93	9.0
94	6.0
95	3.0
96	3.0
97	3.0
98	6.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31319921585754296
2	0.18310679023075785
3	0.2016649108622536
4	0.22275930798005372
5	0.25047276812308733
6	0.20011840080962892
7	0.19597375386859492
8	0.21836721943059972
9	0.21855280063691468
10	0.2272751173337177
11	0.2033970021211932
12	0.20890257790853692
13	0.2211509375253241
14	0.2097686235380067
15	0.20086072563488877
16	0.20352072292540319
17	0.20469607056539788
18	0.2073560678559123
19	0.20475793096750286
20	0.20352072292540319
21	0.20178863166646355
22	0.20525281418434277
23	0.20822211348538208
24	0.22269744757794874
25	0.21774861540954987
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1616543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.228415635958893
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.14076316076395	13.290131302087074
2	10.617033256788446	3.6582932353749924
3	4.393341280627667	2.2707092883980815
4	2.3329717952207596	1.6077363102012971
5	1.3378640528338415	1.1524638983314943
6	0.8845459532894302	0.914359519942547
7	0.6073699258735341	0.7324815069411552
8	0.40504162074413275	0.5582580313633878
9	0.3084981933419494	0.4783441588053759
>10	1.6146936290530618	5.134072658897389
>50	0.16077495316903834	1.9498964499118199
>100	0.14315085211876383	4.852526146550404
>500	0.017624099883968406	2.073519390055672
>1k	0.022659556993673665	8.666247707155756
>5k	0.003956430586196989	5.251713640147279
>10k+	0.009711238711574427	47.40924675583628
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	115271	7.130710411043814	No Hit
CCCATGTACTCTGCGTTGATACCAC	112377	6.9516864073519855	No Hit
GAGTACATGGGAAGCAGTGGTATCA	53492	3.3090366293998983	No Hit
CATGTACTCTGCGTTGATACCACTG	43983	2.720806065783589	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	36870	2.280793025610825	No Hit
GCGTTGATACCACTGCTTCCCATGT	36192	2.2388516729836447	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	32906	2.0355783916666614	No Hit
TATCAACGCAGAGTACATGGGAAGC	32290	1.9974723839699904	No Hit
GGTATCAACGCAGAGTACATGGGAA	30378	1.8791952951452573	No Hit
GTATCAACGCAGAGTACATGGGAAG	30249	1.8712153032737144	No Hit
ACTCTGCGTTGATACCACTGCTTCC	29413	1.8195000071139462	No Hit
GCTTCCCATGTACTCTGCGTTGATA	19468	1.2042983081798628	No Hit
GTACTCTGCGTTGATACCACTGCTT	16364	1.012283620045987	No Hit
GTGGTATCAACGCAGAGTACATGGG	16220	1.003375722142869	No Hit
ATACCACTGCTTCCCATGTACTCTG	14757	0.912873953863275	No Hit
GATACCACTGCTTCCCATGTACTCT	13905	0.8601688912698271	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	13413	0.829733573434174	No Hit
ACATGGGAAGCAGTGGTATCAACGC	12890	0.7973805831332664	No Hit
CATGGGAAGCAGTGGTATCAACGCA	12679	0.7843280382891146	No Hit
CAGTGGTATCAACGCAGAGTACATG	12580	0.7782038584807208	No Hit
TACCACTGCTTCCCATGTACTCTGC	12126	0.7501192359250574	No Hit
GTTGATACCACTGCTTCCCATGTAC	11761	0.7275401891567376	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	11630	0.7194364764809844	No Hit
GCAGTGGTATCAACGCAGAGTACAT	11471	0.7096006725462917	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	10974	0.6788560527001138	No Hit
CTTCCCATGTACTCTGCGTTGATAC	10763	0.6658035078559618	No Hit
CCATGTACTCTGCGTTGATACCACT	10658	0.6593081656349382	No Hit
CCACTGCTTCCCATGTACTCTGCGT	9693	0.599612877603627	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	8807	0.5448045613386095	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	8717	0.5392371251491609	No Hit
GTACATGGTAAGCAGTGGTATCAAC	8452	0.5228441185913397	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	8318	0.5145548247092716	No Hit
TTCCCATGTACTCTGCGTTGATACC	8282	0.5123278502334921	No Hit
TGATACCACTGCTTCCCATGTACTC	7457	0.4612930184968788	No Hit
ATCAACGCAGAGTACATGGGAAGCA	6784	0.41966096788022345	No Hit
ACCATGTACTCTGCGTTGATACCAC	6612	0.40902097871816584	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	5997	0.3709768314235996	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5632	0.3483977846552798	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	4966	0.3071987568533593	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4384	0.27119600282825757	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	4223	0.2612364780893549	No Hit
CCCCATGTACTCTGCGTTGATACCA	4166	0.2577104351693707	No Hit
AAAAAGTACTCTGCGTTGATACCAC	4054	0.2507820701336123	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3973	0.24577137756310843	No Hit
TCCATGTACTCTGCGTTGATACCAC	3858	0.23865743132103506	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	3838	0.23742022327893536	No Hit
AAAAACAAAAAAAAAAAAAAAAAAA	3542	0.21910954425585957	No Hit
TCAACGCAGAGTACATGGGAAGCAG	3381	0.20915001951695686	No Hit
CTCTGCGTTGATACCACTGCTTCCC	3186	0.19708724110648465	No Hit
CTGCGTTGATACCACTGCTTCCCAT	3153	0.19504584783702011	No Hit
TCCCATGTACTCTGCGTTGATACCA	3053	0.18885980762652152	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	2991	0.18502446269601242	No Hit
AAAGTACTCTGCGTTGATACCACTG	2991	0.18502446269601242	No Hit
GAGTACATGGTAAGCAGTGGTATCA	2972	0.18384911505601767	No Hit
GTACATGGGTAAGCAGTGGTATCAA	2959	0.1830449298286529	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2863	0.17710633122657424	No Hit
GTACATGGAAAGCAGTGGTATCAAC	2761	0.1707965702118657	No Hit
AGTGGTATCAACGCAGAGTACATGG	2745	0.1698068037781859	No Hit
GCTTACCATGTACTCTGCGTTGATA	2613	0.16164123070032782	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA	2393	0.14803194223723093	No Hit
AACGCAGAGTACATGGGAAGCAGTG	2270	0.14042311277831768	No Hit
ATGTACTCTGCGTTGATACCACTGC	2229	0.13788683629201326	No Hit
ACTCTGCGTTGATACCACTGCTTAC	2211	0.1367733490541235	No Hit
TTGATACCACTGCTTCCCATGTACT	2201	0.13615474503307368	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA	2132	0.13188637728782965	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2116	0.13089661085414986	No Hit
GCGTTGATACCACTGCTTACCATGT	2115	0.1308347504520449	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	2024	0.12520545386049117	No Hit
ACGCAGAGTACATGGTAAGCAGTGG	2010	0.12433940823102138	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	1954	0.12087522571314219	No Hit
GAGTACATGGAAAGCAGTGGTATCA	1931	0.1194524364647275	No Hit
ACACAAAAAAAAAAAAAAAAAAAAA	1931	0.1194524364647275	No Hit
TATCAACGCAGAGTACATGGTAAGC	1928	0.11926685525841255	No Hit
CTGCTTACCATGTACTCTGCGTTGA	1854	0.1146891855026436	No Hit
GGTATCAACGCAGAGTACATGGTAA	1803	0.11153430499528934	No Hit
GTATCAACGCAGAGTACATGGTAAG	1702	0.10528640438268577	No Hit
GAAAACAAAAAAAAAAAAAAAAAAA	1683	0.10411105674269104	No Hit
CAACGCAGAGTACATGGGAAGCAGT	1619	0.10015199100797195	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	6.186040210498576E-5	0.0	0.0	0.0	0.0
9	6.186040210498576E-5	0.0	0.0	0.0	0.0
10	1.2372080420997153E-4	0.0	0.0	3.711624126299146E-4	0.0
11	1.2372080420997153E-4	0.0	0.0	4.3302281473490034E-4	0.0
12	1.2372080420997153E-4	0.0	0.0	4.948832168398861E-4	0.0
13	1.2372080420997153E-4	0.0	0.0	5.567436189448719E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	635	0.0	16.924181	1
ACTTGTG	35	0.0021646004	16.292068	5
TACATAA	595	0.0	16.290552	2
GTACATA	795	0.0	14.833973	1
TACATGA	450	0.0	14.782167	2
AGAACAA	1020	0.0	14.7200575	2
TGCGCTA	40	0.0052807606	14.2493725	10
GCGCTAT	40	0.005287281	14.246722	11
TCGAAAC	40	0.005288368	14.246281	16
GCTATCG	55	1.9635362E-4	13.815433	13
TTGTGCG	50	0.0015004383	13.299003	7
TACAAAA	900	0.0	13.198363	2
ACATAAA	1055	0.0	12.700454	3
CCCGTGT	60	4.054252E-4	12.680654	1
CCCAAGT	60	4.054252E-4	12.680654	1
CCTCTGG	60	4.054252E-4	12.680654	1
GAGAACA	1385	0.0	12.291536	1
CCAAGTA	70	1.0879292E-4	12.217914	2
TTTGCGT	55	0.0030695437	12.090377	10
CGCTATC	55	0.003073109	12.088504	12
>>END_MODULE