FastQCFastQC Report
Wed 25 May 2016
SRR1294972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294972_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616543
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1259757.7928641551755815No Hit
GTACATGGGAAGCAGTGGTATCAAC1122566.944201298697282No Hit
GAGTACATGGGAAGCAGTGGTATCA579613.5854907664070796No Hit
CATGTACTCTGCGTTGATACCACTG493753.054357353933672No Hit
ACGCAGAGTACATGGGAAGCAGTGG419252.593497358251528No Hit
GCGTTGATACCACTGCTTCCCATGT390342.414658935766014No Hit
TATCAACGCAGAGTACATGGGAAGC354812.1948689270869997No Hit
ACTCTGCGTTGATACCACTGCTTCC334052.0664467323170492No Hit
GGTATCAACGCAGAGTACATGGGAA330192.042568617104525No Hit
GTATCAACGCAGAGTACATGGGAAG316171.955840333353335No Hit
GTATCAACGCAGAGTACTTTTTTTT285581.7666093633141835No Hit
GGTATCAACGCAGAGTACTTTTTTT255221.5788011825234465No Hit
TATCAACGCAGAGTACTTTTTTTTT227781.4090562391473656No Hit
GCTTCCCATGTACTCTGCGTTGATA214071.32424562786143No Hit
GTGGTATCAACGCAGAGTACATGGG173351.0723500704899283No Hit
GTACTTTTTTTTTTTTTTTTTTTTT162691.0064068818460135No Hit
ACATGGGAAGCAGTGGTATCAACGC161580.9995403772123599No Hit
CATGGGAAGCAGTGGTATCAACGCA160680.9939729410229112No Hit
GTACTCTGCGTTGATACCACTGCTT158060.9777655156714049No Hit
CAGTGGTATCAACGCAGAGTACATG157160.9721980794819562No Hit
GGGAAGCAGTGGTATCAACGCAGAG156540.9683627345514472No Hit
ATACCACTGCTTCCCATGTACTCTG156370.9673111077156623No Hit
GATACCACTGCTTCCCATGTACTCT149360.9239469658400673No Hit
ACGCAGAGTACTTTTTTTTTTTTTT137570.8510135517582892No Hit
GCAGTGGTATCAACGCAGAGTACAT136500.8443944887330557No Hit
CTGCTTCCCATGTACTCTGCGTTGA129950.80387592535429No Hit
CCATGTACTCTGCGTTGATACCACT129180.799112674392206No Hit
TACCACTGCTTCCCATGTACTCTGC128090.7923698905627626No Hit
GCAGAGTACATGGGAAGCAGTGGTA128030.7919987281501327No Hit
CTTCCCATGTACTCTGCGTTGATAC119620.7399741299798397No Hit
GTTGATACCACTGCTTCCCATGTAC112510.6959913840831948No Hit
CCACTGCTTCCCATGTACTCTGCGT109080.6747732661611847No Hit
AAGCAGTGGTATCAACGCAGAGTAC104420.6459463187802613No Hit
ATGGGAAGCAGTGGTATCAACGCAG98830.6113663540035743No Hit
GAGTACTTTTTTTTTTTTTTTTTTT89080.5510524619512132No Hit
GTACATGGTAAGCAGTGGTATCAAC84230.521050166930295No Hit
TTCCCATGTACTCTGCGTTGATACC81300.5029250691135343No Hit
ATCAACGCAGAGTACATGGGAAGCA80070.49531623965462096No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA79320.4906767094967471No Hit
TGATACCACTGCTTCCCATGTACTC75430.4666130130779076No Hit
ACCATGTACTCTGCGTTGATACCAC74430.460426972867409No Hit
GGAAGCAGTGGTATCAACGCAGAGT50820.3143745634975376No Hit
CCCCATGTACTCTGCGTTGATACCA48120.29767225492919147No Hit
GTGGTATCAACGCAGAGTACTTTTT48000.29692993010393165No Hit
GCAGAGTACTTTTTTTTTTTTTTTT46540.28789831139660377No Hit
GGTAAGCAGTGGTATCAACGCAGAG44530.2754643705735016No Hit
AAAAAGTACTCTGCGTTGATACCAC40390.24985416410203748No Hit
TCAACGCAGAGTACATGGGAAGCAG40230.24886439766835775No Hit
TCCATGTACTCTGCGTTGATACCAC38870.2404513829820797No Hit
ATCAACGCAGAGTACTTTTTTTTTT35400.21898582345164957No Hit
TGGGAAGCAGTGGTATCAACGCAGA35310.21842907983270474No Hit
CTGCGTTGATACCACTGCTTCCCAT35270.21818163822428477No Hit
CTCTGCGTTGATACCACTGCTTCCC35060.21688256978008008No Hit
GAGTACATGGTAAGCAGTGGTATCA33340.2062425806180225No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA33100.20475793096750286No Hit
TCCCATGTACTCTGCGTTGATACCA31420.19436538341386528No Hit
GTACATGGGTAAGCAGTGGTATCAA31250.1933137565780805No Hit
AAAGTACTCTGCGTTGATACCACTG30690.1898495740602013No Hit
AGTGGTATCAACGCAGAGTACATGG30270.1872514371717919No Hit
GCTTACCATGTACTCTGCGTTGATA29750.18403469626233265No Hit
AACGCAGAGTACATGGGAAGCAGTG28170.1742607527297449No Hit
GTACATGGAAAGCAGTGGTATCAAC26990.16696122528135657No Hit
ACTCTGCGTTGATACCACTGCTTAC25730.15916681461612836No Hit
ACGCAGAGTACATGGTAAGCAGTGG25130.15545519048982923No Hit
CAACGCAGAGTACATGGGAAGCAGT22620.1399282295614778No Hit
ATGTACTCTGCGTTGATACCACTGC22100.13671148865201854No Hit
GCGTTGATACCACTGCTTACCATGT22040.1363403262393886No Hit
GAGTACATGGAAAGCAGTGGTATCA21790.13479381618676398No Hit
CGCAGAGTACATGGGAAGCAGTGGT21110.13058730884362493No Hit
TTGATACCACTGCTTCCCATGTACT20820.12879335718258034No Hit
ACTGCTTCCCATGTACTCTGCGTTG20670.12786545115100556No Hit
TATCAACGCAGAGTACATGGTAAGC19950.12341150219944659No Hit
CTGCTTACCATGTACTCTGCGTTGA19760.12223615455945187No Hit
ATGGTAAGCAGTGGTATCAACGCAG18900.11691615997842308No Hit
CGTTGATACCACTGCTTCCCATGTA17900.11073011976792452No Hit
CAACGCAGAGTACTTTTTTTTTTTT17780.10998779494266468No Hit
GGTATCAACGCAGAGTACATGGTAA17460.10800826207530514No Hit
GAACAAAAAAAAAAAAAAAAAAAAA17120.10590500840373562No Hit
ACGCAGAGTACATGGAAAGCAGTGG17040.10541012518689573No Hit
GCTTTCCATGTACTCTGCGTTGATA16900.10454407955742594No Hit
GTATCAACGCAGAGTACATGGTAAG16690.10324501111322125No Hit
AACGCAGAGTACTTTTTTTTTTTTT16430.1016366406584916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGCA855.638867E-1116.7633698
GCCGAGT601.4681209E-615.8320719
GTACAAA3200.014.5677941
GGGACGC1006.6029315E-1014.2488637
CGTGTCG400.005282019314.24886310
TGGGACG1051.371518E-913.5703466
TACAAAA3500.013.0275332
GTCTTGG550.003035687612.1082951
CGCAAAA2050.012.0478192
GTAAAAA951.0219537E-612.0172571
AAAAAGT6100.011.85308551
AGAACAA2650.011.8292452
AAAAGTA6200.011.7974462
GTATCGA1052.722736E-711.76096618
TACATAA658.029099E-411.6913752
ACGTGTC658.029099E-411.6913758
ACGCAAA3200.011.2974731
GAACAAA5750.011.2509381
CGAGTAC855.327259E-511.1755811
ACTATGT700.001492451610.85627658
Produced by FastQC (version 0.11.5)