FastQCFastQC Report
Wed 25 May 2016
SRR1294971_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294971_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5356859
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC653.3269753E-614.635941
ATACCGT1950.011.6902676
GGTATCA23200.011.0305771
GACCGTG1951.4551915E-1110.2289837
GTATCAA46250.09.7499091
TATCCCG1304.2462943E-69.4995845
GTCTACG1554.1809108E-79.2064781
GTACTAG3100.09.2064781
GTTCTAG5800.09.1853131
GTCTAAG3550.09.1113871
AGACCGT1901.0630174E-88.9984296
ATTAGAC2750.08.9824313
GTCTAGA6250.08.9806131
GTACCGA850.00745036838.9396166
CGGTACT1401.085268E-58.8221144
CGTAGAC1401.08539625E-58.8220323
GTATAGA8350.08.77279951
TATACTG10400.08.6775055
CGCTCAT1107.161945E-48.63485610
GATAGCG1107.161945E-48.6348567
Produced by FastQC (version 0.11.5)