FastQCFastQC Report
Wed 25 May 2016
SRR1294971_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294971_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5356859
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT129700.24211949577168262No Hit
GGTATCAACGCAGAGTACTTTTTTT79070.14760515443844985No Hit
TATCAACGCAGAGTACTTTTTTTTT73310.13685258469562106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29050.013.0878461
AACCGTC752.0770807E-411.39913757
CGACCGC856.603146E-410.05806310
TCGACCG856.603146E-410.0580639
GTATCAA59700.09.9508461
CTAGACT4300.09.7214654
TTAGACG1201.7011977E-59.5005244
TAGCCCG800.0045164459.5005235
GGACCGC1502.650322E-79.5005236
GACCGCT1252.7594087E-59.119317
TGGACCG2106.693881E-109.0481175
ATCGACC1054.499417E-49.0469348
TACGCTG850.00743635468.94166855
TTAGACT3300.08.9247334
TAGACAG8000.08.9067415
GTCTTAG4500.08.8713781
GTATTAA10250.08.6240921
TTTAGAC4300.08.6169953
TATACTG10050.08.6024645
CCTATAC5250.08.5054683
Produced by FastQC (version 0.11.5)