FastQCFastQC Report
Wed 25 May 2016
SRR1294970_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294970_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3945947
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC126380.3202779966380694No Hit
CCCATGTACTCTGCGTTGATACCAC112880.2860656770098534No Hit
GAGTACATGGGAAGCAGTGGTATCA55970.14184174293268512No Hit
GTATCAACGCAGAGTACTTTTTTTT51170.12967736262043053No Hit
GTATCAACGCAGAGTACATGGGAAG40310.10215545216395457No Hit
GCGTTGATACCACTGCTTCCCATGT39740.10071093200187432No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTT500.001502533613.2973287
GCAACGT550.003074506512.088488
GTATTAG5400.010.0438491
AACACCG900.00111468049.4996585
GTCCTAA2850.09.3482911
TTAGACA4600.09.2945594
TAGGACA4950.09.2131924
TACACCG1054.4969667E-49.0472935
GTCTAAC2002.584784E-99.0394641
TAGGACT4100.09.0376364
CTTAGAC2701.8189894E-128.7972073
GTCTTAG3700.08.7437211
CACACCG1107.1510306E-48.6360525
ACACCGA1107.1627676E-48.6346286
TTACGGT1000.0029033988.5509934
CCGTCCT1000.00291174288.5482839
GTATTAA8300.08.4834541
CTAGGAC3600.08.4453183
GCACCGT1701.617911E-68.380676
GTGTAGA6150.08.3548331
Produced by FastQC (version 0.11.5)