FastQCFastQC Report
Wed 25 May 2016
SRR1294970_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294970_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3945947
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT154000.3902738683515009No Hit
GTACATGGGAAGCAGTGGTATCAAC125420.3178451205756185No Hit
CCCATGTACTCTGCGTTGATACCAC120550.3055033430504768No Hit
GGTATCAACGCAGAGTACTTTTTTT93290.2364197998604644No Hit
TATCAACGCAGAGTACTTTTTTTTT85710.21721021595069576No Hit
GAGTACATGGGAAGCAGTGGTATCA56140.1422725647354108No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45770.11599243476914413No Hit
CATGTACTCTGCGTTGATACCACTG41660.10557668412677615No Hit
GCGTTGATACCACTGCTTCCCATGT40740.10324517790026069No Hit
GTATCAACGCAGAGTACATGGGAAG40690.10311846560534137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCTA400.005282924514.24922114
GTACCGT855.3290372E-511.1761436
GGCGCGT600.005880770311.0827278
AGTACCG1409.706673E-810.1782725
TACCCCG750.00264976210.1330365
TAACGCT750.00264976210.1330364
CGTCCGC750.002650319110.13277910
GAACCGT856.599517E-410.0585286
GTCCTAA3600.09.5058651
GTGTACG1002.7402167E-49.5058641
ACGCTAA800.0045191439.4997226
TAGCGGA900.00111487049.4994819
TATTCCG1651.0675285E-79.2118515
TAGACAG5400.08.9719585
GTATTAG6150.08.8103131
TATACAG8300.08.698545
CGTGCAA1107.152361E-48.63589210
TAACCCG1000.0029072268.5497495
GTCTAGG3600.08.4496571
GCCTTAC2605.820766E-118.4090341
Produced by FastQC (version 0.11.5)