FastQCFastQC Report
Wed 25 May 2016
SRR1294969_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294969_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2398367
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC79440.3312253712630302No Hit
CCCATGTACTCTGCGTTGATACCAC68680.28636151181199543No Hit
GTATCAACGCAGAGTACTTTTTTTT36870.15372960018212392No Hit
GAGTACATGGGAAGCAGTGGTATCA35490.1479756851224187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA26010.1084487903644438No Hit
GTATCAACGCAGAGTACATGGGAAG25380.1058220030545784No Hit
CATGTACTCTGCGTTGATACCACTG24930.1039457264046745No Hit
GGTATCAACGCAGAGTACTTTTTTT24390.10169419442478987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGCG807.708877E-915.4393992
GACAGCG853.956069E-612.2912687
TGGTCGG752.0779719E-411.39807110
AGACCGT855.338006E-511.1738816
CTGGTCG855.3391406E-511.1736489
TATTACG700.001490382610.8584792
ATACCGT1002.4060064E-510.4475786
GTCTCGC1105.9351314E-610.3800771
GTCTAGC1855.820766E-1110.2865641
GCCGCGG951.6426267E-410.001233
GTGTAGG3500.09.786931
TAGCACC1502.6439375E-79.5013674
GATAGCC1054.504132E-49.0455237
GTCCTAT1701.645094E-78.955361
CGTGCAC850.0074297148.9422763
GTGTTAC1502.6451726E-68.8807331
GTCTTAG2409.822543E-118.7221491
TTACTCC1752.6202542E-78.6869644
ATAAGAC3950.08.6592923
GTCCTAG2201.5588739E-98.6500641
Produced by FastQC (version 0.11.5)