FastQCFastQC Report
Wed 25 May 2016
SRR1294969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2398367
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT106570.4443440057339014No Hit
GTACATGGGAAGCAGTGGTATCAAC78050.32542976116666045No Hit
CCCATGTACTCTGCGTTGATACCAC76320.3182165198236967No Hit
GGTATCAACGCAGAGTACTTTTTTT72240.3012049448645683No Hit
TATCAACGCAGAGTACTTTTTTTTT59970.2500451348771894No Hit
GAGTACATGGGAAGCAGTGGTATCA35990.15006043695564522No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35560.1482675503790704No Hit
CATGTACTCTGCGTTGATACCACTG26220.10932438613439895No Hit
GTATCAACGCAGAGTACATGGGAAG25270.10536335765126854No Hit
GCGTTGATACCACTGCTTCCCATGT25110.10469623706463606No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTA350.002171888516.2843387
TATGCCG456.764281E-414.7777615
ATAGACG701.09096414E-412.2147813
TTTGACG600.005875461311.0837833
TGTACCG600.005877285311.0833225
TAGACGT700.001491928510.8571314
TATTCCG909.519208E-510.5555445
ACACCGT750.002648493310.1333226
CAGGACC2450.010.0816224
GCTAGAC1356.4424967E-79.8522513
CTTACAC3800.09.7503963
TAGACAG4250.09.6117545
CTAGACA2800.09.49998954
CCTTGCG800.00452010949.4991979
GTATTAA4650.09.4048981
CTACACT3550.09.0985814
TTAGACA3050.09.0327774
GACTAGT950.00181180699.006751
CTTTGCG850.00744237478.9404219
GTATAGA3750.08.8733181