FastQCFastQC Report
Wed 25 May 2016
SRR1294968_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294968_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5390925
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC99270.18414279553137913No Hit
CCCATGTACTCTGCGTTGATACCAC86300.16008384460922753No Hit
GTATCAACGCAGAGTACTTTTTTTT68010.12615645737976322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT550.003041871612.1058491
GTATTAA9750.08.975181
GTTCTAG6150.08.970421
TATACTG10750.08.7488335
TAGACAG8100.08.4444415
ACTGCGT1252.750536E-48.3585998
TCTGTCG3300.08.3470868
ACCGCTT1150.00111320528.2594858
TATTCCG2201.46683306E-88.2045415
TACACTG11700.08.1196545
GTATTAG9300.08.0798641
TAGACTG7550.08.0529775
CTGCGTA1304.1964755E-48.0371159
ACCGTAT1304.1964755E-48.0371158
GTCCTAG5350.08.0005271
GTATAGA10000.07.989861
ATAGCAC5950.07.98378233
TAGCACT7500.07.98058844
GTATAGG6800.07.9730751
CTGTGCG1553.910035E-57.9664079
Produced by FastQC (version 0.11.5)