FastQCFastQC Report
Wed 25 May 2016
SRR1294968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294968_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5390925
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT193130.3582502075246827No Hit
GGTATCAACGCAGAGTACTTTTTTT118620.22003645014538323No Hit
TATCAACGCAGAGTACTTTTTTTTT108050.20042942537690656No Hit
GTACATGGGAAGCAGTGGTATCAAC98760.1831967612237232No Hit
CCCATGTACTCTGCGTTGATACCAC94990.17620352722399216No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57740.10710592338049592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA853.9512634E-612.2933258
GCACCGT1459.822543E-1111.7932196
ATCGTCC1104.9804476E-711.2265498
CTCGCGT951.3627843E-510.9992914
TCGCGTT803.776177E-410.68681115
TGTAGCG800.0045181389.50009255
ACTCGCG900.00111510959.49938813
TACACCG1951.6862032E-99.2565015
ACACCGT2501.8189894E-129.120096
CACCGTC1201.7665513E-48.7077727
GTCTTAC6050.08.6413371
GTCTAGG5300.08.6087281
GTATTAG9600.08.4163021
GTTCTAT5900.08.3777041
GTCTCGC2855.456968E-128.3381331
CCGTCCC1150.00111206898.2603379
AACACCG1502.5883846E-58.2334145
GTGCTAG5550.08.2209481
GTATCAA98400.08.1723861
TAGACAG7450.08.1611545
Produced by FastQC (version 0.11.5)