FastQCFastQC Report
Wed 25 May 2016
SRR1294967_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294967_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4825775
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC79060.1638286078401915No Hit
CCCATGTACTCTGCGTTGATACCAC73310.1519134232325378No Hit
GTATCAACGCAGAGTACTTTTTTTT49650.10288502882956622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCG750.002649322910.1333235
CTAGGAC3850.09.6245643
TGCTACG800.0045137619.5011722
ATACCGT1002.7633167E-49.4980196
ACACCGC800.0045261369.4980186
TAGGACC2357.2759576E-129.2990194
TTACGGT1054.4902647E-49.0487364
CGAATAC950.00182285759.0011113
TAAAGCG850.0074395578.9411675
CTGTCGC4600.08.8785849
TAGGACA5250.08.8677614
GTCTTAG5150.08.6843971
GTTACAC3300.08.6374293
CGTACTG1107.1492704E-48.6363545
GTCTACG1000.00286307198.5642941
ATAGGAC4000.08.5510553
TCTGTCG4800.08.5087318
GTCCTAT3600.08.4585611
TGTCGCC4750.08.39816110
ACACCGT1701.618444E-68.3806056
Produced by FastQC (version 0.11.5)